Strain identifier

BacDive ID: 1998

Type strain: No

Species: Cupriavidus necator

Strain Designation: H 1

Strain history: <- IMG, H 1 <- R. Bartha

NCBI tax ID(s): 106590 (species)

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General

@ref: 127

BacDive-ID: 1998

DSM-Number: 529

keywords: Bacteria, aerobe, mesophilic

description: Cupriavidus necator H 1 is an aerobe, mesophilic bacterium that was isolated from sludge from a garden pond.

NCBI tax id

  • NCBI tax id: 106590
  • Matching level: species

strain history

  • @ref: 127
  • history: <- IMG, H 1 <- R. Bartha

doi: 10.13145/bacdive1998.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Cupriavidus
  • species: Cupriavidus necator
  • full scientific name: Cupriavidus necator Makkar and Casida 1987

@ref: 127

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Cupriavidus

species: Cupriavidus necator

full scientific name: Cupriavidus necator Makkar and Casida 1987

strain designation: H 1

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
127MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81)yeshttps://mediadive.dsmz.de/medium/81Name: MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81) Composition: Agar 20.1005 g/l Na2HPO4 x 2 H2O 2.91457 g/l KH2PO4 2.31156 g/l NH4Cl 1.00503 g/l MgSO4 x 7 H2O 0.502512 g/l Ferric ammonium citrate 0.0502513 g/l CaCl2 x 2 H2O 0.0100503 g/l NaVO3 x H2O 0.00502512 g/l Calcium pantothenate 0.00251256 g/l Pyridoxine hydrochloride 0.00251256 g/l Nicotinic acid 0.00251256 g/l Thiamine-HCl x 2 H2O 0.00251256 g/l H3BO3 0.00150754 g/l CoCl2 x 6 H2O 0.00100503 g/l Riboflavin 0.000502513 g/l ZnSO4 x 7 H2O 0.000502513 g/l MnCl2 x 4 H2O 0.000150754 g/l Na2MoO4 x 2 H2O 0.000150754 g/l NiCl2 x 6 H2O 0.000100503 g/l CuCl2 x 2 H2O 5.02513e-05 g/l Vitamin B12 5.02513e-05 g/l Folic acid 1.00503e-05 g/l Biotin 5.02513e-06 g/l Distilled water
127NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
127positivegrowth30mesophilic
59753positivegrowth30-37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 59753
  • oxygen tolerance: aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    59753C14:04.514
    59753C15:00.415
    59753C16:031.416
    59753C18:00.218
    59753C12:0 ALDE ?2.510.915
    59753C14:0 2OH2.315.205
    59753C14:0 3OH/C16:1 ISO I5.215.485
    59753C16:0 2OH0.517.233
    59753C16:1 2OH0.717.047
    59753C16:1 ω5c0.415.908
    59753C16:1 ω7c27.315.819
    59753C17:0 CYCLO12.216.888
    59753C18:1 2OH0.619.088
    59753C18:1 ω7c /12t/9t11.417.824
    59753C18:2 ω6,9c/C18:0 ANTE0.317.724
    59753Unidentified0.218.135
    59753unknown 14.5030.214.503
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
59753-++--+----++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
59753+-----------+-++-++++

Isolation, sampling and environmental information

isolation

@refsample typesampling datecountryorigin.countrycontinent
127sludge from a garden pond
59753Sludge,garden pond1962GermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Garden
#Environmental#Terrestrial#Mud (Sludge)
#Environmental#Aquatic#Pond (small)

Safety information

risk assessment

  • @ref: 127
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 127

culture collection no.: CCUG 52966, LMG 1200, ATCC 23442, DSM 529, ATCC 17698

straininfo link

  • @ref: 71645
  • straininfo: 699

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1334068Molecular and genetic characterization of an Alcaligenes eutrophus insertion element.Kung SS, Chen J, Chow WYJ Bacteriol10.1128/jb.174.24.8023-8029.19921992Alcaligenes/*genetics, Bacterial Proteins/*genetics, Base Sequence, Blotting, Southern, Cloning, Molecular, *DNA Transposable Elements/*genetics, DNA, Bacterial, Molecular Sequence Data, Mutation, Phenotype, Restriction MappingGenetics
Metabolism8495435Microbial synthesis of 3-deoxy-D-erythro-hex-2-ulosonic acid 6-phosphate.Knappmann BR, el-Nawawy MA, Schlegel HG, Kula MRCarbohydr Res10.1016/0008-6215(93)80029-e1993Alcaligenes/*metabolism, Aldehyde-Lyases/genetics, Escherichia coli/*metabolism, Gluconates/*chemical synthesis/chemistry/isolation & purification/metabolism, Kinetics, Magnetic Resonance Spectroscopy, Pyruvates/metabolism, Pyruvic AcidEnzymology

Reference

@idauthorscataloguedoi/urltitle
127Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 529)https://www.dsmz.de/collection/catalogue/details/culture/DSM-529
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
59753Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 52966)https://www.ccug.se/strain?id=52966
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
71645Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID699.1StrainInfo: A central database for resolving microbial strain identifiers