[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Stock solution 1 (in 100ml): (NH4)2SO4 0.5g K2HPO4 7.5g
Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g
Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g
Basal medium: Agar 1.5%
Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5%
Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Na-glutamate 0.5% Yeast extract (Difco) 0.1% MgSO4 x 7H2O 0.1% Agar 1.5%
Preparation / Remarks: pH 7.2 after autoclaving add: Glucose 0.2% (autoclaved separately) Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamine B12 0.5 µg/ml
Preparation / Remarks: pH 7.2 autoclaved Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
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Ingredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416. *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml
Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on cy-plates. *
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[Ref.: #18281] |
Culture medium |
STAN 21 AGAR with Filter * | |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: 1. Agar 1% K2HPO4 0.1% Yeast extract (Difco) 0.002%
2. (NH4)2SO4 0.1% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% MnSO4 x H2O 0.01% FeCl3 x 6H2O 0.02% MnSO4 x 7H2O 0.01% FeCl3 0.02% Traceelement-solution 1ml to 100 ml
Preparation / Remarks: 1. in 2/3 of the volume after autoclaving 1 + 2 together and pour plates. Hang up a sterile-filter paper *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Agar (Difco) 1.5% CaCl2 0.1% Cycloheximide 2.5 mg per 100 ml
Preparation / Remarks: pH 7.2 (KOH) autoclaved w-agar with 2.5 mg of cycloheximide per 100 ml, the antibiotic is filter sterilized and added to the agar just before pouring Cycloheximide does not seem to affect growth of myxobakteria, whereas fungi and amoebae are often inhibited. Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) Pour the plates and streak autoclaved E.coli cells *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 %
Preparation / Remarks: pH 7.2 autoclaved *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 %
Preparation / Remarks: pH 7.2 autoclaved Combine with 250 mg/L Kanamycin-sulfate and 125 mg/L Streptomycin-sulfate (each sterilized by filtration) *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 %
Preparation / Remarks: pH 7.2 autoclaved Combine with 250 mg/L Kanamycin-sulfate, 250 mg/L Neomycin-sulfate, 250 mg/L Cycloheximide (each sterilized by filtration) and 250 mg/L Trimethoprim . Solve the Trimethoprim in 30 mL Methanol (p.a.) in a sterile vessel and add without sterilization. *
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