[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Stock solution 1 (in 100ml): (NH4)2SO4 0.5g K2HPO4 7.5g
Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g
Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g
Basal medium: Agar 1.5%
Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g
Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g
Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g
Basal medium: Agar 1.5%
Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5%
Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Casitone(Difco) 0.3% yeast extract 0.1% CaCl2 0.1% Agar 1.5%
Preparation / Remarks: pH 7.2 (KOH) autoclaved *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamine B12 0.5 µg/ml
Preparation / Remarks: pH 7.2 autoclaved Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416. *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Solution 1 Ingredients: Yeast extract (Difco) 0.05 % MgSO4 x 7H2O 0.1 % CaCl2 x 2H2O 0.1 % Vitamine B12 0.1 mg/L Agar 1.5 %
Preparation / Remarks: Solve in 90 % of the water. pH 7.2 autoclaved
Solution 2 Ingredients: Skim milk (Oxoid) 15g
Preparation / Remarks: Solve in 10 % of the the water pH 7.2 Autoclave exactly 15 min at 115 °C. Mix the two solutions and pour plates. *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Solution 1 Ingredients: Yeast extract (Difco) 0.05 % MgSO4 x 7H2O 0.1 % CaCl2 x 2H2O 0.1 % Vitamine B12 0.1 mg/L Agar 1.5 %
Preparation / Remarks: Solve in 90 % of the water. pH 7.2 autoclaved
Solution 2 Ingredients: Skim milk (Oxoid) 0.2 %
Preparation / Remarks: Solve in 10 % of the the water pH 7.2 Autoclave exactly 15 min at 115 °C. Mix the two solutions and pour plates. *
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[Ref.: #18281] |
Culture medium |
STAN 21 AGAR with Filter * | |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: 1. Agar 1% K2HPO4 0.1% Yeast extract (Difco) 0.002%
2. (NH4)2SO4 0.1% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% MnSO4 x H2O 0.01% FeCl3 x 6H2O 0.02% MnSO4 x 7H2O 0.01% FeCl3 0.02% Traceelement-solution 1ml to 100 ml
Preparation / Remarks: 1. in 2/3 of the volume after autoclaving 1 + 2 together and pour plates. Hang up a sterile-filter paper *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Agar (Difco) 1.5% CaCl2 0.1% Cycloheximide 2.5 mg per 100 ml
Preparation / Remarks: pH 7.2 (KOH) autoclaved w-agar with 2.5 mg of cycloheximide per 100 ml, the antibiotic is filter sterilized and added to the agar just before pouring Cycloheximide does not seem to affect growth of myxobakteria, whereas fungi and amoebae are often inhibited. Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) Pour the plates and streak autoclaved E.coli cells *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 %
Preparation / Remarks: pH 7.2 autoclaved *
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