Name: Bifidobacterium breve S50 (Variant a)
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1696

Type strain:

Species: Bifidobacterium breve

Strain Designation: S50 (Variant a), S50

Culture col. no.: DSM 20091, ATCC 15698, JCM 1273, CCUG 24611, CIP 64.66, KCTC 3461, LMG 10737, NCFB 2258, NCDO 2258, CCUG 45220

Strain history: CIP <- 1964, Lab. Ident. Inst. Pasteur, Paris, France <- M. Sebald, Inst. Pasteur, Paris, France <- H. Werner <- G. Reuter: strain S50

NCBI tax ID(s): 1385941 (strain), 1685 (species)

SI-ID 125611

LMG 13194, ATCC 15698, CCUG 24611, DSM 20091, JCM 1273, LMG 10737, NCFB 2258, NCDO 2258, CCUG 45220, NCIMB 702258, CIP 64.66, KCTC 3461, BCRC 14601, CCRC 14601

Special Collections

Other strains from Bifidobacterium breve

Section

Bifidobacterium breve S50 is an anaerobe, Gram-positive human pathogen that was isolated from intestine of infant.

Gram-positive
anaerobe
human pathogen
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Bifidobacteriales*
	Family	*Bifidobacteriaceae*
	Genus	*Bifidobacterium*
	Species	**Bifidobacterium breve**
	Full Scientific Name (LPSN)	Bifidobacterium breve Reuter 1963 (Approved Lists 1980)

Information on morphological and physiological properties Morphology

[Ref.: #117002]	Gram stain	positive

[Ref.: #117002]	Cell shape	rod-shaped

[Ref.: #117002]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %91

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8517]

Culture medium

BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)

[Ref.: #8517]

Culture medium growth

[Ref.: #8517]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8517]

Culture medium composition

expand / minimize

[Ref.: #33435]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #33435]

Culture medium growth

[Ref.: #33435]

Culture medium composition

expand / minimize

[Ref.: #117002]

Culture medium

CIP Medium 20

[Ref.: #117002]

Culture medium growth

[Ref.: #117002]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8517]	growth	37 °C
[Ref.: #33435]	growth	37 °C
[Ref.: #67770]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8517]	Oxygen tolerance	anaerobe
[Ref.: #117002]	Oxygen tolerance	anaerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

[Ref.: #8517]	Murein short key	A11.01
[Ref.: #8517]	Murein types	A3alpha L-Lys-Gly

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #117002]	nitrate ChEBI	-	reduction
[Ref.: #117002]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #117002]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #117002]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #117002]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #117002]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #8517]	14519	-	-	-	+	-	+	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	+	+	+/-	+/-	+	+	+	+	+	+	-	-	-	-	+	+	+	-	-	-	-	-	-	+	-	-	-	-	-
[Ref.: #8517]	14518	-	-	-	+	-	+	-	-	-	-	+	+	+	+	-	-	-	-	-	+	-	+	+	+	+	+	+	+	+	+	+	+	-	-	-	+	+	+	-	+	+	-	-	-	+	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #117002]	13064	-	-	+	-	-	+	-	-	-	-	-	-	-	+	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8517]	Sample type/isolated from	intestine of infant

[Ref.: #67770]	Sample type/isolated from	Intestine of infant

[Ref.: #117002]	Sample type/isolated from	Human, Intestine of infant
* marker position based on {}

Isolation sources categories

#Host	#Human	#Child
#Host Body-Site	#Gastrointestinal tract	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8517]	Pathogenicity (human)	yes, in single cases
[Ref.: #8517]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #117002]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	B.breve (ATCC 15698) DNA sequence for 16S rRNA	X70972	1464	GenBank ENA	1685 tax ID	*
[Ref.: #124043]	Bifidobacterium breve gene for 16S rRNA, partial sequence, strain: JCM 1273.	AB507085	607	GenBank ENA	1685 tax ID	*
[Ref.: #20218]	Bifidobacterium breve ATCC15698 16S-23S intergenic spacer	U09518	488	GenBank ENA	1685 tax ID	*
[Ref.: #124043]	Bifidobacterium breve strain JCM 1273 16S ribosomal RNA gene, partial sequence.	AF491832	1454	GenBank ENA	1685 tax ID	*
[Ref.: #124043]	Bifidobacterium breve gene for 16S rRNA, partial sequence, strain: JCM 1273.	AB116289	479	GenBank ENA	1685 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bifidobacterium breve NCFB 2258	GCA_000569035	complete	GenBank ENA	1385941 tax ID	*
[Ref.: #66792]	Bifidobacterium breve NCFB 2258	1385941.3	complete	PATRIC	1385941 tax ID	*
[Ref.: #66792]	Bifidobacterium breve strain NCFB 2258	1685.70	plasmid	PATRIC	1685 tax ID	*
[Ref.: #66792]	Bifidobacterium breve NCFB 2258	2565956583	complete	JGI IMG/M	1385941 tax ID	*

[Ref.: #8517]	GC-content	59.4 mol%
[Ref.: #67770]	GC-content	59.4 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bifidobacterium breve NCFB 2258 NCFB 2258 NCBI: GCA_000569035.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bifidobacterium breve NCFB 2258 NCFB 2258 NCBI: GCA_000569035.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	87.079	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	82.585	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	92.158	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	86.766	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	92.965	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	91	no

Availability in culture collections External links

[Ref.: #8517]

Culture collection no.

DSM 20091, ATCC 15698, JCM 1273, CCUG 24611, CIP 64.66, KCTC 3461, LMG 10737, NCFB 2258, NCDO 2258, CCUG 45220

[Ref.: #71343]

SI-ID 125611

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Structural Identity of Galactooligosaccharide Molecules Selectively Utilized by Single Cultures of Probiotic Bacterial Strains.	Boger M, van Leeuwen SS, Lammerts van Bueren A, Dijkhuizen L	J Agric Food Chem	10.1021/acs.jafc.9b05968	2019	*
Metabolism	Identification and characterization of an oleate hydratase-encoding gene from Bifidobacterium breve.	O'Connell KJ, Motherway MO, Hennessey AA, Brodhun F, Ross RP, Feussner I, Stanton C, Fitzgerald GF, van Sinderen D	Bioengineered	10.4161/bioe.24159	2013	*
Pathogenicity	Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.	Ruiz L, Motherway MO, Lanigan N, van Sinderen D	PLoS One	10.1371/journal.pone.0064699	2013	*
Pathogenicity	Prebiotic-non-digestible oligosaccharides preference of probiotic bifidobacteria and antimicrobial activity against Clostridium difficile.	Kondepudi KK, Ambalam P, Nilsson I, Wadstrom T, Ljungh A	Anaerobe	10.1016/j.anaerobe.2012.08.005	2012	*
Metabolism	Mechanism of conjugated linoleic acid and vaccenic acid formation in human faecal suspensions and pure cultures of intestinal bacteria.	McIntosh FM, Shingfield KJ, Devillard E, Russell WR, Wallace RJ	Microbiology (Reading)	10.1099/mic.0.022921-0	2009	*
Genetics	Characterization of plasmid pASV479 from Bifidobacterium pseudolongum subsp. globosum and its use for expression vector construction.	Sangrador-Vegas A, Stanton C, van Sinderen D, Fitzgerald GF, Ross RP	Plasmid	10.1016/j.plasmid.2007.02.004	2007	*
Genetics	Molecular characterisation of a 5.75-kb cryptic plasmid from Bifidobacterium breve NCFB 2258 and determination of mode of replication.	O'Riordan K, Fitzgerald GF	FEMS Microbiol Lett	10.1111/j.1574-6968.1999.tb13581.x	1999	*
Enzymology	Viability and enzymatic activity of bifidobacteria in milk.	Hughes DB, Hoover DG	J Dairy Sci	10.3168/jds.S0022-0302(95)76634-6	1995	*
Enzymology	Analysis of the genome of the five Bifidobacterium breve strains: plasmid content, pulsed-field gel electrophoresis genome size estimation and rrn loci number.	Bourget N, Simonet JM, Decaris B	FEMS Microbiol Lett	10.1111/j.1574-6968.1993.tb06288.x	1993	*

References

#8517

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20091

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#33435 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71343

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID125611.1 )

#117002 Collection of Institut Pasteur ; Curators of the CIP; CIP 64.66

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

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Bifidobacterium breve NCFB 2258 NCFB 2258

Bifidobacterium breve NCFB 2258 NCFB 2258

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Change proposal