Bifidobacterium breve (DSM 20091, ATCC 15698, S50 (Variant a))

Name: Bifidobacterium breve (DSM 20091, ATCC 15698, S50 (Variant a))
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1696

Type strain

Strain Designation S50 (Variant a), S50

Culture col. no. DSM 20091 ATCC 15698 S50 (Variant a) NCFB 2258 LMG 10737 7 more

NCBI tax ID(s) 1385941 1685

Special Collections DSMZ CCUG JCM KCTC CIP

History <- G. Reuter, S50 (Bifidobacterium parvulorum) (Variant a) DSM 20091 <-- G. Reuter S50 ("Bifidobacterium parvulorum") (Variant a). CIP <- 1964, Lab. Ident. Inst. Pasteur, Paris, France <- M. Sebald, Inst. Pasteur, Paris, France <- H. Werner <- G. Reuter: strain S50

Links

Bifidobacterium breve S50 is an anaerobe, Gram-positive, rod-shaped bacterium that was isolated from intestine of infant.

Gram-positive rod-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 125611

LMG 13194,ATCC 15698,CCUG 24611,DSM 20091,JCM 1273,LMG 10737,NCFB 2258,NCDO 2258,CCUG 45220,NCIMB 702258,CIP 64.66,KCTC 3461,BCRC 14601,CCRC 14601

@ref 20215

Last LPSN update 2026-02-09 11:18:09

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Bifidobacteriales

Family Bifidobacteriaceae

Genus Bifidobacterium

Species Bifidobacterium breve

Full scientific name Bifidobacterium breve Reuter 1963 (Approved Lists 1980)

BacDive ID	Other strains from **Bifidobacterium breve** (35)	Type strain
1695	B. breve S1, DSM 20213, ATCC 15700, NCTC 11815, JCM ... (type strain)
134790	B. breve 17c, CIP 64.68, ATCC 15699, CCUG 45221
137497	B. breve S46, CIP 64.70, ATCC 15701, JCM 7016, BCRC ...
138672	B. breve 755-84, CIP 101158
141735	B. breve CCUG 5862, NCTC 10471
143052	B. breve CCUG 16568
143053	B. breve CCUG 16569
144929	B. breve CCUG 27171 A
146282	B. breve CCUG 30785
146947	B. breve CCUG 32526 B
147417	B. breve CCUG 33558
147864	B. breve CCUG 34405
148335	B. breve CCUG 35244
148336	B. breve CCUG 35245
150885	B. breve CCUG 42620
150897	B. breve CCUG 42657
151165	B. breve CCUG 43395 A
151166	B. breve CCUG 43395 B
151681	B. breve CCUG 44623
152514	B. breve CCUG 46983
152771	B. breve CCUG 47591
152812	B. breve CCUG 47672
152865	B. breve CCUG 47820
152875	B. breve CCUG 47844
153826	B. breve CCUG 51291
154347	B. breve CCUG 53196
154385	B. breve CCUG 53338
154927	B. breve CCUG 55738
154963	B. breve CCUG 55883
155699	B. breve CCUG 59045
155780	B. breve CCUG 59290
165454	B. breve JCM 7017
165455	B. breve JCM 7019
165456	B. breve JCM 7020
176408	B. breve HUN447, RP2, DSM 118819

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
117002	positive	rod-shaped
125438				91

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8517	BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)	Medium recipe at MediaDive	Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water
33435	MEDIUM 20 - for Anaerobic bacteria		Agar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
117002	CIP Medium 20	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
8517	positive	growth	37
33435	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
8517	anaerobe
117002	anaerobe

Spore formation

@ref	Spore formation	Confidence
125439		92.1

Murein

@ref	Murein short key	Type
8517	A11.01	A3alpha L-Lys-Gly

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	+	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	+	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	+	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	+	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
117002	17632 ChEBI	nitrate	-	reduction
117002	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
117002	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
117002	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
117002	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
117002	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
117002	-	-	+	-	-	+	-	-	-	-	-	-	-	+	-	-	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
8517	-	-	-	+	-	+	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	+	+	+/-	+/-	+	+	+	+	+	+	-	-	-	-	+	+	+	-	-	-	-	-	-	+	-	-	-	-	-
8517	-	-	-	+	-	+	-	-	-	-	+	+	+	+	-	-	-	-	-	+	-	+	+	+	+	+	+	+	+	+	+	+	-	-	-	+	+	+	-	+	+	-	-	-	+	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Human	#Child
#Host Body-Site	#Gastrointestinal tract	-

Isolation

@ref	Sample type
8517	intestine of infant
67770	Intestine of infant
117002	Human, Intestine of infant

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
8517	yes, in single cases	1	Risk group (German classification) According to TRBA 466
117002		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM56903v1 assembly for Bifidobacterium breve NCFB 2258	complete	GCA_000569035	1385941.3	2565956583	1385941	99.11

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Bifidobacterium breve ATCC15698 16S-23S intergenic spacer	U09518	488	1685
20218	B.breve (ATCC 15698) DNA sequence for 16S rRNA	X70972	1464	1685
124043	Bifidobacterium breve gene for 16S rRNA, partial sequence, strain: JCM 1273.	AB116289	479	1685
124043	Bifidobacterium breve gene for 16S rRNA, partial sequence, strain: JCM 1273.	AB507085	607	1685
124043	Bifidobacterium breve strain JCM 1273 16S ribosomal RNA gene, partial sequence.	AF491832	1454	1685

GC content

@ref	GC-content (mol%)	Method
8517	59.4
67770	59.4	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Bifidobacterium breve NCFB 2258
NCBI: GCA_000569035

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	92.10	no
125439	motility	BacteriaNetⓘ	no	89.30	no
125439	gram_stain	BacteriaNetⓘ	positive	88.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	71.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Bifidobacterium breve NCFB 2258 NCFB 2258
NCBI: GCA_000569035.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.08	no
125438	anaerobic	anaerobicⓘ	yes	82.59	no
125438	aerobic	aerobicⓘ	no	92.16	yes
125438	spore-forming	spore-formingⓘ	no	86.77	no
125438	thermophilic	thermophileⓘ	no	92.97	yes
125438	flagellated	motile2+ⓘ	no	91.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Investigating the potential of yacon (Smallanthus sonchifolius) juice in the development of organic apple-based snacks.	Braschi G, Njieukam JA, Gottardi D, Genovese J, Tylewicz U, Patrignani F, Rocculi P.	Heliyon	10.1016/j.heliyon.2024.e32342	2024
	Microbial Conjugated Linolenic Acid-Enriched Fermented Milk Using Lipase-Hydrolyzed Flaxseed Oil: Biochemical, Organoleptic and Storage Traits.	Fontes AL, Pimentel LL, Monteiro MJP, Domingues MR, Rodriguez-Alcala LM, Gomes AM.	Foods	10.3390/foods13010021	2023
	Short-communication: Study of fatty acid metabolites in microbial conjugated fatty acids-enrichment of milk and discovery of additional undescribed conjugated linolenic acid isomers.	Fontes AL, Neves B, Conde T, Couto D, Pimentel LL, Rodriguez-Alcala LM, Domingues MR, Gomes AM.	Food Chem	10.1016/j.foodchem.2023.137276	2024
	Study of the viability of using lipase-hydrolyzed commercial vegetable oils to produce microbially conjugated linolenic acid-enriched milk.	Fontes AL, Pimentel LL, Soares AMS, Domingues MDR, Rodriguez-Alcala LM, Gomes AM.	Food Chem	10.1016/j.foodchem.2023.135665	2023
Phylogeny	Comparison of various molecular methods for rapid differentiation of intestinal bifidobacteria at the species, subspecies and strain level.	Jarocki P, Podlesny M, Komon-Janczara E, Kucharska J, Glibowska A, Targonski Z.	BMC Microbiol	10.1186/s12866-016-0779-3	2016
Enzymology	A new insight into the physiological role of bile salt hydrolase among intestinal bacteria from the genus Bifidobacterium.	Jarocki P, Podlesny M, Glibowski P, Targonski Z.	PLoS One	10.1371/journal.pone.0114379	2014
Enzymology	Genetic diversity of bile salt hydrolases among human intestinal bifidobacteria.	Jarocki P, Targonski Z.	Curr Microbiol	10.1007/s00284-013-0362-1	2013
	Cross-feeding of bifidobacteria promotes intestinal homeostasis: a lifelong perspective on the host health.	Xiao M, Zhang C, Duan H, Narbad A, Zhao J, Chen W, Zhai Q, Yu L, Tian F.	NPJ Biofilms Microbiomes	10.1038/s41522-024-00524-6	2024
	DNA enrichment and tagmentation method for species-level identification and strain-level differentiation using ON-rep-seq.	Krych L, Castro-Mejia JL, Forero-Junco LM, Moesby DN, Mikkelsen MB, Rasmussen MA, Sykulski M, Nielsen DS.	Commun Biol	10.1038/s42003-019-0617-x	2019
Enzymology	Quantitative real-time PCR assays to identify and quantify fecal Bifidobacterium species in infants receiving a prebiotic infant formula.	Haarman M, Knol J.	Appl Environ Microbiol	10.1128/aem.71.5.2318-2324.2005	2005
Metabolism	Intestinal integrity and Akkermansia muciniphila, a mucin-degrading member of the intestinal microbiota present in infants, adults, and the elderly.	Collado MC, Derrien M, Isolauri E, de Vos WM, Salminen S.	Appl Environ Microbiol	10.1128/aem.01477-07	2007
	Exploring the Potential Probiotic Properties of Bifidobacterium breve DSM 32583-A Novel Strain Isolated from Human Milk.	Kujawska M, Neuhaus K, Huptas C, Jimenez E, Arboleya S, Schaubeck M, Hall LJ.	Probiotics Antimicrob Proteins	10.1007/s12602-024-10346-9	2025
	Bacterial metabolites influence the autofluorescence of Clostridioides difficile.	Ticer TD, Tingler AM, Glover JS, Dooley SA, Kendrick J, Zackular JP, Devkota S, Wu GD, Mahalak K, Engevik A, Engevik MA.	Front Microbiol	10.3389/fmicb.2024.1459795	2024
Enzymology	Safety Evaluation of Bifidobacterium breve IDCC4401 Isolated from Infant Feces for Use as a Commercial Probiotic.	Choi IY, Kim J, Kim SH, Ban OH, Yang J, Park MK.	J Microbiol Biotechnol	10.4014/jmb.2103.03041	2021
	Growth and survival of Bifidobacterium breve and Bifidobacterium longum in various sugar systems with fructooligosaccharide supplementation.	Parhi P, Song KP, Choo WS.	J Food Sci Technol	10.1007/s13197-022-05361-z	2022
	Evaluation of the microbiota-sparing properties of the anti-staphylococcal antibiotic afabicin.	Nowakowska J, Cameron DR, De Martino A, Kuhn J, Le Fresne-Languille S, Leuillet S, Amouzou Y, Wittke F, Carton T, Le Vacon F, Chaves RL, Nicolas-Metral V, Vuagniaux G.	J Antimicrob Chemother	10.1093/jac/dkad181	2023
	Identifying single-strain growth patterns of human gut microbes in response to preterm human milk and formula.	Engevik MA, Stripe LK, Baatz JE, Wagner CL, Chetta KE.	Food Funct	10.1039/d2fo00447j	2022
Metabolism	Bifidobacterium dentium Fortifies the Intestinal Mucus Layer via Autophagy and Calcium Signaling Pathways.	Engevik MA, Luk B, Chang-Graham AL, Hall A, Herrmann B, Ruan W, Endres BT, Shi Z, Garey KW, Hyser JM, Versalovic J.	mBio	10.1128/mbio.01087-19	2019
	Mechanisms involved in alleviation of intestinal inflammation by bifidobacterium breve soluble factors.	Heuvelin E, Lebreton C, Grangette C, Pot B, Cerf-Bensussan N, Heyman M.	PLoS One	10.1371/journal.pone.0005184	2009
Metabolism	Bifidobacteria shape host neural circuits during postnatal development by promoting synapse formation and microglial function.	Luck B, Engevik MA, Ganesh BP, Lackey EP, Lin T, Balderas M, Major A, Runge J, Luna RA, Sillitoe RV, Versalovic J.	Sci Rep	10.1038/s41598-020-64173-3	2020
	Breast milk urea as a nitrogen source for urease positive Bifidobacterium infantis.	Schimmel P, Kleinjans L, Bongers RS, Knol J, Belzer C.	FEMS Microbiol Ecol	10.1093/femsec/fiab019	2021
	Design, Synthesis, and Characterization of TNP-2198, a Dual-Targeted Rifamycin-Nitroimidazole Conjugate with Potent Activity against Microaerophilic and Anaerobic Bacterial Pathogens.	Ma Z, He S, Yuan Y, Zhuang Z, Liu Y, Wang H, Chen J, Xu X, Ding C, Molodtsov V, Lin W, Robertson GT, Weiss WJ, Pulse M, Nguyen P, Duncan L, Doyle T, Ebright RH, Lynch AS.	J Med Chem	10.1021/acs.jmedchem.1c02045	2022
Genetics	Technological advances in bifidobacterial molecular genetics: application to functional genomics and medical treatments.	Fukiya S, Hirayama Y, Sakanaka M, Kano Y, Yokota A.	Biosci Microbiota Food Health	10.12938/bmfh.31.15	2012
	Probiotics: progress toward novel therapies for intestinal diseases.	Yan F, Polk DB.	Curr Opin Gastroenterol	10.1097/mog.0b013e328335239a	2010
Phylogeny	Identification of Bifidobacterium strains by rRNA gene restriction patterns.	Mangin I, Bourget N, Bouhnik Y, Bisetti N, Simonet JM, Decaris B.	Appl Environ Microbiol	10.1128/aem.60.5.1451-1458.1994	1994
	Postnatal colonization with human "infant-type" Bifidobacterium species alters behavior of adult gnotobiotic mice.	Luk B, Veeraragavan S, Engevik M, Balderas M, Major A, Runge J, Luna RA, Versalovic J.	PLoS One	10.1371/journal.pone.0196510	2018
Enzymology	Detection of Bifidobacterium animalis subsp. lactis (Bb12) in the intestine after feeding of sows and their piglets.	Solano-Aguilar G, Dawson H, Restrepo M, Andrews K, Vinyard B, Urban JF.	Appl Environ Microbiol	10.1128/aem.00309-08	2008
Enzymology	A simple method for quantification of uncultured microorganisms in the environment based on in vitro transcription of 16S rRNA.	Polz MF, Polz MF, Cavanaugh CM.	Appl Environ Microbiol	10.1128/aem.63.3.1028-1033.1997	1997
Enzymology	Identification, detection, and enumeration of human bifidobacterium species by PCR targeting the transaldolase gene.	Requena T, Burton J, Matsuki T, Munro K, Simon MA, Tanaka R, Watanabe K, Tannock GW.	Appl Environ Microbiol	10.1128/aem.68.5.2420-2427.2002	2002
Pathogenicity	Novel riboswitch-binding flavin analog that protects mice against Clostridium difficile infection without inhibiting cecal flora.	Blount KF, Megyola C, Plummer M, Osterman D, O'Connell T, Aristoff P, Quinn C, Chrusciel RA, Poel TJ, Schostarez HJ, Stewart CA, Walker DP, Wuts PG, Breaker RR.	Antimicrob Agents Chemother	10.1128/aac.01282-15	2015
Enzymology	Species-specific oligonucleotide probes for five Bifidobacterium species detected in human intestinal microflora.	Yamamoto T, Morotomi M, Tanaka R.	Appl Environ Microbiol	10.1128/aem.58.12.4076-4079.1992	1992
Enzymology	Cloning and characterization of the bile salt hydrolase genes (bsh) from Bifidobacterium bifidum strains.	Kim GB, Miyamoto CM, Meighen EA, Lee BH.	Appl Environ Microbiol	10.1128/aem.70.9.5603-5612.2004	2004
Pathogenicity	Bifidobacterium breve Exopolysaccharide Blocks Dendritic Cell Maturation and Activation of CD4⁺ T Cells.	Hickey A, Stamou P, Udayan S, Ramon-Vazquez A, Esteban-Torres M, Bottacini F, Woznicki JA, Hughes O, Melgar S, Ventura M, Van Sinderen D, Rossini V, Nally K.	Front Microbiol	10.3389/fmicb.2021.653587	2021
Pathogenicity	Lactobacilli Cell-Free Supernatants Modulate Inflammation and Oxidative Stress in Human Microglia via NRF2-SOD1 Signaling.	Di Chiano M, Rocchetti MT, Spano G, Russo P, Allegretta C, Milior G, Gadaleta RM, Sallustio F, Pontrelli P, Gesualdo L, Avolio C, Fiocco D, Gallone A.	Cell Mol Neurobiol	10.1007/s10571-024-01494-1	2024
	Evaluating the Impact of Probiotic Therapy on the Endocannabinoid System, Pain, Sleep and Fatigue: A Randomized, Double-Blind, Placebo-Controlled Trial in Dancers.	Wiacek J, Podgorski T, Kusy K, Loniewski I, Skonieczna-Zydecka K, Karolkiewicz J.	Int J Mol Sci	10.3390/ijms25115611	2024
	Immunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteria.	Morrin ST, McCarthy G, Kennedy D, Marotta M, Irwin JA, Hickey RM.	AMB Express	10.1186/s13568-020-01048-w	2020
	Effects of Linoleic Acid on Gut-Derived Bifidobacterium breve DSM 20213: A Transcriptomic Approach.	Senizza A, Callegari ML, Senizza B, Minuti A, Rocchetti G, Morelli L, Patrone V.	Microorganisms	10.3390/microorganisms7120710	2019
	Maximum depth sequencing reveals an ON/OFF replication slippage switch and apparent in vivo selection for bifidobacterial pilus expression.	Penno C, Motherway MO, Fu Y, Sharma V, Crispie F, Cotter PD, Houeix B, Joshi L, Bottacini F, O'Dwyer A, Loughran G, Atkins JF, van Sinderen D.	Sci Rep	10.1038/s41598-022-13668-2	2022
	Discovery of a conjugative megaplasmid in Bifidobacterium breve.	Bottacini F, O'Connell Motherway M, Casey E, McDonnell B, Mahony J, Ventura M, van Sinderen D.	Appl Environ Microbiol	10.1128/aem.02871-14	2015
Genetics	Comparative genomics of the Bifidobacterium breve taxon.	Bottacini F, O'Connell Motherway M, Kuczynski J, O'Connell KJ, Serafini F, Duranti S, Milani C, Turroni F, Lugli GA, Zomer A, Zhurina D, Riedel C, Ventura M, van Sinderen D.	BMC Genomics	10.1186/1471-2164-15-170	2014
Metabolism	Linoleic acid induces metabolic stress in the intestinal microorganism Bifidobacterium breve DSM 20213.	Senizza A, Rocchetti G, Callegari ML, Lucini L, Morelli L.	Sci Rep	10.1038/s41598-020-62897-w	2020
Enzymology	Microbial Production of Conjugated Linoleic Acid and Conjugated Linolenic Acid Relies on a Multienzymatic System.	Salsinha AS, Pimentel LL, Fontes AL, Gomes AM, Rodriguez-Alcala LM.	Microbiol Mol Biol Rev	10.1128/mmbr.00019-18	2018
Enzymology	Bifidobacteria isolated from vaginal and gut microbiomes are indistinguishable by comparative genomics.	Freitas AC, Hill JE.	PLoS One	10.1371/journal.pone.0196290	2018
	Immunostimulatory activity of potential probiotic yeast strains in the dorsal air pouch system and the gut mucosa.	Kourelis A, Kotzamanidis C, Litopoulou-Tzanetaki E, Papaconstantinou J, Tzanetakis N, Yiangou M.	J Appl Microbiol	10.1111/j.1365-2672.2009.04651.x	2010
	Validation of the dorsal air pouch model to predict and examine immunostimulatory responses in the gut.	Kourelis A, Zinonos I, Kakagianni M, Christidou A, Christoglou N, Yiannaki E, Testa T, Kotzamanidis C, Litopoulou-Tzanetaki E, Tzanetakis N, Yiangou M.	J Appl Microbiol	10.1111/j.1365-2672.2009.04421.x	2010
Genetics	Genome-Wide Assessment of Stress-Associated Genes in Bifidobacteria.	Schopping M, Vesth T, Jensen K, Franzen CJ, Zeidan AA.	Appl Environ Microbiol	10.1128/aem.02251-21	2022
	Intestinal survival and persistence of probiotic Lactobacillus and Bifidobacterium strains administered in triple-strain yoghurt	Matto J, Fonden R, Tolvanen T, Wright Av, Vilpponen-Salmela T, Satokari R, Saarela M.	Int Dairy J	10.1016/j.idairyj.2005.10.007	2006
Metabolism	Administration of Lactobacillus evokes coordinated changes in the intestinal expression profile of genes regulating energy homeostasis and immune phenotype in mice.	Nerstedt A, Nilsson EC, Ohlson K, Hakansson J, Thomas Svensson L, Lowenadler B, Svensson UK, Mahlapuu M.	Br J Nutr	10.1017/s0007114507682907	2007
Enzymology	Probiotic lactobacilli and bacteraemia in Stockholm.	Sullivan A, Nord CE.	Scand J Infect Dis	10.1080/00365540500449826	2006
Metabolism	Metabolism of four alpha-glycosidic linkage-containing oligosaccharides by Bifidobacterium breve UCC2003.	O'Connell KJ, O'Connell Motherway M, O'Callaghan J, Fitzgerald GF, Ross RP, Ventura M, Stanton C, van Sinderen D.	Appl Environ Microbiol	10.1128/aem.01775-13	2013
Metabolism	Influence of controlled lactic fermentation on growth and sporulation of Bacillus cereus in milk.	Rossland E, Langsrud T, Sorhaug T.	Int J Food Microbiol	10.1016/j.ijfoodmicro.2004.11.027	2005
Metabolism	Screening for and identification of starch-, amylopectin-, and pullulan-degrading activities in bifidobacterial strains.	Ryan SM, Fitzgerald GF, van Sinderen D.	Appl Environ Microbiol	10.1128/aem.00257-06	2006
Enzymology	Evaluation of bifidobacteria for the production of antimicrobial compounds and assessment of performance in cottage cheese at refrigeration temperature.	O'Riordan K, Fitzgerald GF.	J Appl Microbiol	10.1046/j.1365-2672.1998.00474.x	1998
Enzymology	Mining the microbiota of the neonatal gastrointestinal tract for conjugated linoleic acid-producing bifidobacteria.	Rosberg-Cody E, Ross RP, Hussey S, Ryan CA, Murphy BP, Fitzgerald GF, Devery R, Stanton C.	Appl Environ Microbiol	10.1128/aem.70.8.4635-4641.2004	2004
	Characterization and induction of prophages in human gut-associated Bifidobacterium hosts.	Mavrich TN, Casey E, Oliveira J, Bottacini F, James K, Franz CMAP, Lugli GA, Neve H, Ventura M, Hatfull GF, Mahony J, van Sinderen D.	Sci Rep	10.1038/s41598-018-31181-3	2018
Enzymology	Metabolism of linoleic acid by human gut bacteria: different routes for biosynthesis of conjugated linoleic acid.	Devillard E, McIntosh FM, Duncan SH, Wallace RJ.	J Bacteriol	10.1128/jb.01359-06	2007
Enzymology	Growth, viability, and proteolytic activity of bifidobacteria in whole camel milk.	Abu-Taraboush HM, al-Dagal MM, al-Royli MA.	J Dairy Sci	10.3168/jds.s0022-0302(98)75584-5	1998
Enzymology	Overcoming the restriction barrier to plasmid transformation and targeted mutagenesis in Bifidobacterium breve UCC2003.	O'Connell Motherway M, O'Driscoll J, Fitzgerald GF, Van Sinderen D.	Microb Biotechnol	10.1111/j.1751-7915.2008.00071.x	2009
Metabolism	Diversity, ecology and intestinal function of bifidobacteria.	Bottacini F, Ventura M, van Sinderen D, O'Connell Motherway M.	Microb Cell Fact	10.1186/1475-2859-13-s1-s4	2014
Metabolism	Metabolism of a plant derived galactose-containing polysaccharide by Bifidobacterium breve UCC2003.	O'Connell Motherway M, Fitzgerald GF, van Sinderen D.	Microb Biotechnol	10.1111/j.1751-7915.2010.00218.x	2011
	Characterization of GH2 and GH42 beta-galactosidases derived from bifidobacterial infant isolates.	Ambrogi V, Bottacini F, O'Sullivan J, O'Connell Motherway M, Linqiu C, Schoemaker B, Schoterman M, van Sinderen D.	AMB Express	10.1186/s13568-019-0735-3	2019
	Effect of supplement with lactic-acid producing bacteria on fatigue and physical activity in patients with chronic fatigue syndrome.	Sullivan A, Nord CE, Evengard B.	Nutr J	10.1186/1475-2891-8-4	2009
Metabolism	Effects of rice bran oil on the intestinal microbiota and metabolism of isoflavones in adult mice.	Tamura M, Hori S, Hoshi C, Nakagawa H.	Int J Mol Sci	10.3390/ijms130810336	2012
Pathogenicity	Effects of antibiotics on bacterial species composition and metabolic activities in chemostats containing defined populations of human gut microorganisms.	Newton DF, Macfarlane S, Macfarlane GT.	Antimicrob Agents Chemother	10.1128/aac.00079-13	2013
Metabolism	Induction of sucrose utilization genes from Bifidobacterium lactis by sucrose and raffinose.	Trindade MI, Abratt VR, Reid SJ.	Appl Environ Microbiol	10.1128/aem.69.1.24-32.2003	2003
	Monitoring of antibiotic-induced alterations in the human intestinal microflora and detection of probiotic strains by use of terminal restriction fragment length polymorphism.	Jernberg C, Sullivan A, Edlund C, Jansson JK.	Appl Environ Microbiol	10.1128/aem.71.1.501-506.2005	2005
Metabolism	Contribution of surface beta-glucan polysaccharide to physicochemical and immunomodulatory properties of Propionibacterium freudenreichii.	Deutsch SM, Parayre S, Bouchoux A, Guyomarc'h F, Dewulf J, Dols-Lafargue M, Bagliniere F, Cousin FJ, Falentin H, Jan G, Foligne B.	Appl Environ Microbiol	10.1128/aem.07027-11	2012
Metabolism	Regulation of exopolysaccharide production by Lactococcus lactis subsp. cremoris By the sugar source.	Looijesteijn PJ, Boels IC, Kleerebezem M, Hugenholtz J.	Appl Environ Microbiol	10.1128/aem.65.11.5003-5008.1999	1999
Metabolism	Effect of Pufa Substrates on Fatty Acid Profile of Bifidobacterium breve Ncimb 702258 and CLA/CLNA Production in Commercial Semi-Skimmed Milk.	Fontes AL, Pimentel L, Rodriguez-Alcala LM, Gomes A.	Sci Rep	10.1038/s41598-018-33970-2	2018
	Fructooligosaccharides for Relieving Functional Constipation: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.	Zhen H, Qian H, Liu X, Tan C.	Foods	10.3390/foods13243993	2024
	Novel Dietary Approach with Probiotics, Prebiotics, and Synbiotics to Mitigate Antimicrobial Resistance and Subsequent Out Marketplace of Antimicrobial Agents: A Review.	Habteweld HA, Asfaw T.	Infect Drug Resist	10.2147/idr.s413416	2023
	Comparison of Yacon (Smallanthus sonchifolius) Tuber with Commercialized Fructo-oligosaccharides (FOS) in Terms of Physiology, Fermentation Products and Intestinal Microbial Communities in Rats.	Utami NW, Sone T, Tanaka M, Nakatsu CH, Saito A, Asano K.	Biosci Microbiota Food Health	10.12938/bmfh.32.167	2013
Metabolism	Structural Identity of Galactooligosaccharide Molecules Selectively Utilized by Single Cultures of Probiotic Bacterial Strains.	Boger M, van Leeuwen SS, Lammerts van Bueren A, Dijkhuizen L	J Agric Food Chem	10.1021/acs.jafc.9b05968	2019
Metabolism	Identification and characterization of an oleate hydratase-encoding gene from Bifidobacterium breve.	O'Connell KJ, Motherway MO, Hennessey AA, Brodhun F, Ross RP, Feussner I, Stanton C, Fitzgerald GF, van Sinderen D	Bioengineered	10.4161/bioe.24159	2013
Pathogenicity	Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.	Ruiz L, Motherway MO, Lanigan N, van Sinderen D	PLoS One	10.1371/journal.pone.0064699	2013
Pathogenicity	Prebiotic-non-digestible oligosaccharides preference of probiotic bifidobacteria and antimicrobial activity against Clostridium difficile.	Kondepudi KK, Ambalam P, Nilsson I, Wadstrom T, Ljungh A	Anaerobe	10.1016/j.anaerobe.2012.08.005	2012
Metabolism	Mechanism of conjugated linoleic acid and vaccenic acid formation in human faecal suspensions and pure cultures of intestinal bacteria.	McIntosh FM, Shingfield KJ, Devillard E, Russell WR, Wallace RJ	Microbiology (Reading)	10.1099/mic.0.022921-0	2009
Genetics	Characterization of plasmid pASV479 from Bifidobacterium pseudolongum subsp. globosum and its use for expression vector construction.	Sangrador-Vegas A, Stanton C, van Sinderen D, Fitzgerald GF, Ross RP	Plasmid	10.1016/j.plasmid.2007.02.004	2007
Genetics	Molecular characterisation of a 5.75-kb cryptic plasmid from Bifidobacterium breve NCFB 2258 and determination of mode of replication.	O'Riordan K, Fitzgerald GF	FEMS Microbiol Lett	10.1111/j.1574-6968.1999.tb13581.x	1999
Enzymology	Viability and enzymatic activity of bifidobacteria in milk.	Hughes DB, Hoover DG	J Dairy Sci	10.3168/jds.S0022-0302(95)76634-6	1995
Enzymology	Analysis of the genome of the five Bifidobacterium breve strains: plasmid content, pulsed-field gel electrophoresis genome size estimation and rrn loci number.	Bourget N, Simonet JM, Decaris B	FEMS Microbiol Lett	10.1111/j.1574-6968.1993.tb06288.x	1993

References

#8517	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20091
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#33435	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#117002	Collection of Institut Pasteur ; Curators of the CIP; CIP 64.66
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Bifidobacterium breve (DSM 20091, ATCC 15698, S50 (Variant a))

Name and taxonomic classification

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Physiology and metabolism

Oxygen tolerance

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Murein

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