Bifidobacterium breve (CIP 64.68, ATCC 15699, CCUG 45221)

Name: Bifidobacterium breve (CIP 64.68, ATCC 15699, CCUG 45221)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 134790

Type strain

Strain Designation 17c

Culture col. no. CIP 64.68 ATCC 15699 CCUG 45221 17c

NCBI tax ID(s) 1685

Special Collections CCUG CIP

History CIP <- 1964, Lab. Ident. Inst. Pasteur, Paris, France <- M. Sebald, Inst. Pasteur, Paris, France <- H. Werner <- G. Reuter: strain 17c

Links

Bifidobacterium breve 17c is an anaerobe, Gram-positive, rod-shaped bacterium that was isolated from Human, Faeces.

Gram-positive rod-shaped anaerobe Bacteria

Name and taxonomic classification

SI-ID 67577

ATCC 15699,CCUG 45221,CIP 64.68,KCTC 3372,VTT E-991418

@ref 20215

Last LPSN update 2026-02-09 11:32:17

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Bifidobacteriales

Family Bifidobacteriaceae

Genus Bifidobacterium

Species Bifidobacterium breve

Full scientific name Bifidobacterium breve Reuter 1963 (Approved Lists 1980)

BacDive ID	Other strains from **Bifidobacterium breve** (35)	Type strain
1695	B. breve S1, DSM 20213, ATCC 15700, NCTC 11815, JCM ... (type strain)
1696	B. breve S50 (Variant a), S50, DSM 20091, ATCC 15698, ...
137497	B. breve S46, CIP 64.70, ATCC 15701, JCM 7016, BCRC ...
138672	B. breve 755-84, CIP 101158
141735	B. breve CCUG 5862, NCTC 10471
143052	B. breve CCUG 16568
143053	B. breve CCUG 16569
144929	B. breve CCUG 27171 A
146282	B. breve CCUG 30785
146947	B. breve CCUG 32526 B
147417	B. breve CCUG 33558
147864	B. breve CCUG 34405
148335	B. breve CCUG 35244
148336	B. breve CCUG 35245
150885	B. breve CCUG 42620
150897	B. breve CCUG 42657
151165	B. breve CCUG 43395 A
151166	B. breve CCUG 43395 B
151681	B. breve CCUG 44623
152514	B. breve CCUG 46983
152771	B. breve CCUG 47591
152812	B. breve CCUG 47672
152865	B. breve CCUG 47820
152875	B. breve CCUG 47844
153826	B. breve CCUG 51291
154347	B. breve CCUG 53196
154385	B. breve CCUG 53338
154927	B. breve CCUG 55738
154963	B. breve CCUG 55883
155699	B. breve CCUG 59045
155780	B. breve CCUG 59290
165454	B. breve JCM 7017
165455	B. breve JCM 7019
165456	B. breve JCM 7020
176408	B. breve HUN447, RP2, DSM 118819

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
33436	positive	rod-shaped

Colony morphology

@ref	Incubation period
56698	2 days
33436

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition	Medium link
33436	MEDIUM 20 - for Anaerobic bacteria		Agar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
33436	CIP Medium 20			Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
33436	positive	growth	37
56698	positive	growth	37

Physiology and metabolism

Oxygen tolerance

56698

Oxygen toleranceanaerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68381	40585 ChEBI	alpha-cyclodextrin	-	builds acid from	from API rID32STR
68381	29016 ChEBI	arginine	-	hydrolysis	from API rID32STR
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
33436	17057 ChEBI	cellobiose	-	degradation
68381	18333 ChEBI	D-arabitol	-	builds acid from	from API rID32STR
33436	15824 ChEBI	D-fructose	-	degradation
33436	17634 ChEBI	D-glucose	+	degradation
68381	16899 ChEBI	D-mannitol	-	builds acid from	from API rID32STR
68380	16024 ChEBI	D-mannose	+	fermentation	from API rID32A
68381	16988 ChEBI	D-ribose	+	builds acid from	from API rID32STR
68381	16443 ChEBI	D-tagatose	-	builds acid from	from API rID32STR
33436	4853 ChEBI	esculin	-	hydrolysis
68381	28087 ChEBI	glycogen	+	builds acid from	from API rID32STR
68381	606565 ChEBI	hippurate	-	hydrolysis	from API rID32STR
68381	30849 ChEBI	L-arabinose	-	builds acid from	from API rID32STR
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
68381	17716 ChEBI	lactose	+	builds acid from	from API rID32STR
33436	17716 ChEBI	lactose	-	degradation
68381	17306 ChEBI	maltose	+	builds acid from	from API rID32STR
33436	17306 ChEBI	maltose	-	degradation
68381	6731 ChEBI	melezitose	-	builds acid from	from API rID32STR
68381	28053 ChEBI	melibiose	+	builds acid from	from API rID32STR
68381	320055 ChEBI	methyl beta-D-glucopyranoside	+	builds acid from	from API rID32STR
33436	17632 ChEBI	nitrate	-	reduction
33436	17632 ChEBI	nitrate	+	respiration
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
33436	16301 ChEBI	nitrite	-	reduction
68381	27941 ChEBI	pullulan	+	builds acid from	from API rID32STR
68381	16634 ChEBI	raffinose	+	builds acid from	from API rID32STR
68380	16634 ChEBI	raffinose	+	fermentation	from API rID32A
33436	17814 ChEBI	salicin	+	degradation
68381	30911 ChEBI	sorbitol	-	builds acid from	from API rID32STR
68381	17992 ChEBI	sucrose	+	builds acid from	from API rID32STR
33436	17992 ChEBI	sucrose	-	degradation
68381	27082 ChEBI	trehalose	+	builds acid from	from API rID32STR
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
68381	16199 ChEBI	urea	-	hydrolysis	from API rID32STR
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A

Metabolite production

@ref	Chebi-ID	Metabolite
68381	15688 ChEBI	acetoin	from API rID32STR
68380	35581 ChEBI	indole	from API rID32A
33436	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68381	15688 ChEBI	acetoin	-		from API rID32STR
68380	35581 ChEBI	indole		-	from API rID32A

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68380	alanine arylamidase	-	3.4.11.2	from API rID32A
68381	Alanyl-Phenylalanyl-Proline arylamidase	+		from API rID32STR
68381	alkaline phosphatase	-	3.1.3.1	from API rID32STR
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68380	alkaline phosphatase	-	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	+	3.2.1.55	from API rID32A
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68381	alpha-galactosidase	+	3.2.1.22	from API rID32STR
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68380	alpha-galactosidase	+	3.2.1.22	from API rID32A
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68380	alpha-glucosidase	+	3.2.1.20	from API rID32A
68382	alpha-mannosidase	-	3.2.1.24	from API zym
33436	amylase	-
68381	arginine dihydrolase	-	3.5.3.6	from API rID32STR
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
68381	beta-galactosidase	+	3.2.1.23	from API rID32STR
68382	beta-galactosidase	-	3.2.1.23	from API zym
33436	beta-galactosidase	+	3.2.1.23
68380	beta-galactosidase	+	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68381	beta-glucosidase	+	3.2.1.21	from API rID32STR
68382	beta-glucosidase	-	3.2.1.21	from API zym
68380	beta-glucosidase	+	3.2.1.21	from API rID32A
68381	beta-glucuronidase	-	3.2.1.31	from API rID32STR
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
68381	beta-mannosidase	-	3.2.1.25	from API rID32STR
33436	caseinase	-	3.4.21.50
33436	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
33436	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
33436	gelatinase	-
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	+		from API rID32A
68381	glycyl tryptophan arylamidase	-		from API rID32STR
68380	histidine arylamidase	+		from API rID32A
68380	L-arginine arylamidase	+		from API rID32A
33436	lecithinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
68380	leucine arylamidase	+	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
33436	lipase	-
68382	lipase (C 14)	-		from API zym
68381	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32STR
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68380	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API rID32A
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
33436	oxidase	-
68380	phenylalanine arylamidase	+		from API rID32A
68380	proline-arylamidase	+	3.4.11.5	from API rID32A
68381	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32STR
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	+		from API rID32A
68382	trypsin	-	3.4.21.4	from API zym
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
33436	tween esterase	-
68380	tyrosine arylamidase	+		from API rID32A
68381	urease	-	3.5.1.5	from API rID32STR
33436	urease	-	3.5.1.5
68380	urease	-	3.5.1.5	from API rID32A
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
33436	-	+	+	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
56698	-	-	+	+	-	+	+	+	-	+	+	+	-	-	-	-	-	+	+	-	+	+	-	+	-	+	+	-	+

API rID32STR

@ref	ADH (Arg)	beta GLU	beta GAR	beta GUR	alpha GAL	PAL	RIB	MAN	SOR	LAC	TRE	RAF	SAC	LARA	DARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	HIP	GLYG	PUL	MAL	MEL	MLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	TAG	beta MAN	URE
56698	-	+	+	-	+	-	+	-	-	+	+	+	+	-	-	-	-	+	+	-	-	-	-	+	+	+	+	-	+	-	-	-

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
33436	Human, Faeces	Germany	DEU	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
33436	1	Risk group (French classification)

Sequence information

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Adhesion of bifidobacteria to granular starch and its implications in probiotic technologies.	Crittenden R, Laitila A, Forssell P, Matto J, Saarela M, Mattila-Sandholm T, Myllarinen P.	Appl Environ Microbiol	10.1128/aem.67.8.3469-3475.2001	2001
	Exploring the Potential Probiotic Properties of Bifidobacterium breve DSM 32583-A Novel Strain Isolated from Human Milk.	Kujawska M, Neuhaus K, Huptas C, Jimenez E, Arboleya S, Schaubeck M, Hall LJ.	Probiotics Antimicrob Proteins	10.1007/s12602-024-10346-9	2025
Enzymology	Safety Evaluation of Bifidobacterium breve IDCC4401 Isolated from Infant Feces for Use as a Commercial Probiotic.	Choi IY, Kim J, Kim SH, Ban OH, Yang J, Park MK.	J Microbiol Biotechnol	10.4014/jmb.2103.03041	2021
Phylogeny	Identification of Bifidobacterium strains by rRNA gene restriction patterns.	Mangin I, Bourget N, Bouhnik Y, Bisetti N, Simonet JM, Decaris B.	Appl Environ Microbiol	10.1128/aem.60.5.1451-1458.1994	1994

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#33436	Collection of Institut Pasteur ; Curators of the CIP; CIP 64.68
#56698	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 45221
#68380	Automatically annotated from API rID32A .
#68381	Automatically annotated from API rID32STR .
#68382	Automatically annotated from API zym .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Bifidobacterium breve (CIP 64.68, ATCC 15699, CCUG 45221)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API rID32A

API rID32STR

Isolation, sampling and environmental information

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome-based predictions

Literature

Literature

References

BacDive

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