Lactococcus lactis (CIP 103449, JCM 7638)

Name: Lactococcus lactis (CIP 103449, JCM 7638)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 136206

Type strain

Culture col. no. CIP 103449 JCM 7638

NCBI tax ID(s) 1358

Special Collections JCM CIP

History A. Ishizaki IO-1. CIP <- 1990, JCM <- strain O-1, Lactobacillus xylosus

Links

Lactococcus lactis CIP 103449 is a facultative anaerobe, mesophilic, Gram-positive prokaryote of the family Streptococcaceae.

Gram-positive coccus-shaped facultative anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 65145

CIP 103449,JCM 7638

@ref 20215

Last LPSN update 2026-02-09 11:32:36

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Streptococcaceae

Genus Lactococcus

Species Lactococcus lactis

Full scientific name Lactococcus lactis (Lister 1873) Schleifer et al. 1986

Synonyms (4)

Streptococcus lactis subsp. diacetilactis
Lactobacillus xylosus
Streptococcus lactis
"Bacterium lactis"

BacDive ID	Other strains from **Lactococcus lactis** (46)	Type strain
14702	L. lactis OJ, N30, DSM 20481, ATCC 19435, CCM 1877, ... (type strain)
14694	L. lactis Citrate plasmid, F7/2, DSM 4366
14699	L. lactis 117, DSM 20175, ATCC 15577, CCUG 21962, NRRL ...
14700	L. lactis 54296, DSM 20384
14701	L. lactis 5, DSM 20250
14703	L. lactis 2, DSM 20661, NCDO 176
14704	L. lactis Berridge X 13, DSM 20729, ATCC 11454, CCM ...
14705	L. lactis E9, DSM 30863
100031	L. lactis STI07582(IMET), 1136-53,
100032	L. lactis STI07652(IMET), IMET 07690, Mer MER6468
100033	L. lactis STI07667(IMET), M18,
100034	L. lactis STI07670(IMET), RS/20,
134825	L. lactis N34, RICE, CIP 70.57
134851	L. lactis CIP A216
135401	L. lactis H 214, N35, CIP 70.58
138955	L. lactis J4 conserves, CIP A217
138966	L. lactis J4 Alger, CIP A218
139482	L. lactis 129.86, CIP 102103
143933	L. lactis CCUG 21956, NCIMB 10493, ATCC 11007, LMG ...
145429	L. lactis CCUG 28474
146507	L. lactis CCUG 31342
146700	L. lactis CCUG 31972
146846	L. lactis CCUG 32225
148267	L. lactis CCUG 35127 B
149871	L. lactis CCUG 38585
150979	L. lactis CCUG 42927
151600	L. lactis CCUG 44423
152071	L. lactis CCUG 45692
152072	L. lactis CCUG 45693
152073	L. lactis CCUG 45695
152621	L. lactis CCUG 47216
152790	L. lactis CCUG 47618
154162	L. lactis CCUG 52621
154377	L. lactis CCUG 53296
155625	L. lactis CCUG 58504
155919	L. lactis CCUG 59643
156113	L. lactis CCUG 60423
156342	L. lactis CCUG 61221
160084	L. lactis SV21, DSM 27303
160085	L. lactis SB 17, DSM 111832
160086	L. lactis SB 352, DSM 111868
160087	L. lactis SB 150, DSM 111869
160088	L. lactis SB 261, DSM 111870
162165	L. lactis JCM 18008, FERM AP-21288
169254	L. lactis CLA-AV-14, DSM 110082
174486	L. lactis A537/21, DSM 117780

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
125438			92.5
125438	positive		93.758
35548	positive	coccus-shaped

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition	Medium link
35548	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
35548	CIP Medium 40			Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
35548	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
35548	facultative anaerobe
125439	obligate aerobe	95.9

Compound production

67770

CompoundNisin Z

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	+	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	+	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
35548	17632 ChEBI	nitrate	-	reduction
35548	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	+	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
35548	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
35548	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
35548	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
35548	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
35548	-	-	-	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
35548	not determinedn.d.	+/-	-	-	-	+	+	-	-	-	+	+	+	+	-	-	-	-	+	-	+/-	+/-	+	+	+	+	+	+	+	+	-	+	+	-	-	-	+	-	-	+	-	-	-	-	-	-	-	+	-	+/-

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
35548		Fukuoaka-shi	Japan	JPN	Asia
67770	Water in the drain pit of a kitchen sink	Fukuoka	Japan	JPN	Asia
35548	Environment, Water in a drain pit of a kitchen sink	Fukuoka	Japan	JPN	Asia	1986

Taxonmaps

69479

Global distribution of 16S sequence LC096207 (>99% sequence identity) for Lactococcus from Microbeatlas

Aquatic Samples	13841
Soil Samples	7135
Animal Samples	132033
Plant Samples	4677
Total Samples	157686

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
35548	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM34457v1 assembly for Lactococcus lactis subsp. lactis IO-1	complete	GCA_000344575	1046624.4	2563366570	1046624	99.54
66792	ASM131131v1 assembly for Lactococcus lactis subsp. lactis JCM 7638	contig	GCA_001311315	1291762.3	2728369680	1291762	2.31

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
67770	Lactococcus lactis subsp. lactis gene for 16S ribosomal RNA, partial sequence, strain: JCM 7638	LC096207	1486		1360
124043	Lactococcus lactis gene for 16S rRNA, partial sequence, strain:JCM7638.	AB118037	1532		1358

GC content

@ref	GC-content (mol%)	Method
67770	38	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lactococcus lactis subsp. lactis JCM 7638
NCBI: GCA_001311315

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	95.90	no
125439	gram_stain	BacteriaNetⓘ	negative	77.50	no
125439	motility	BacteriaNetⓘ	no	66.40	no
125439	spore_formation	BacteriaNetⓘ	no	85.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactococcus lactis subsp. lactis JCM 7638
PATRIC: 1291762.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	93.76	no
125438	anaerobic	anaerobicⓘ	no	89.75	no
125438	spore-forming	spore-formingⓘ	no	87.07	no
125438	aerobic	aerobicⓘ	no	96.15	no
125438	thermophilic	thermophileⓘ	no	98.50	yes
125438	flagellated	motile2+ⓘ	no	92.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	A Novel C-Terminal Truncated Bacteriocin Found by Comparison between Leuconostoc mesenteroides 406 and 213M0 Isolated from Mongolian Traditional Fermented Milk, Airag.	Hasiqimuge, Hano C, Arakawa K, Yoshida S, Zhao J, Toh H, Morita H, Miyamoto T.	Microorganisms	10.3390/microorganisms12091781	2024
	Isolation, Characterization, and Comparative Genomics of the Novel Potential Probiotics from Canine Feces.	Foongsawat N, Sunthornthummas S, Nantavisai K, Surachat K, Rangsiruji A, Sarawaneeyaruk S, Insian K, Sukontasing S, Suwannasai N, Pringsulaka O.	Food Sci Anim Resour	10.5851/kosfa.2023.e28	2023
Genetics	From Genome to Phenotype: An Integrative Approach to Evaluate the Biodiversity of Lactococcus lactis.	Laroute V, Tormo H, Couderc C, Mercier-Bonin M, Le Bourgeois P, Cocaign-Bousquet M, Daveran-Mingot ML.	Microorganisms	10.3390/microorganisms5020027	2017
Biotechnology	Lactic acid production - producing microorganisms and substrates sources-state of art.	Abedi E, Hashemi SMB.	Heliyon	10.1016/j.heliyon.2020.e04974	2020
Enzymology	Purification, identification, and effective production of a peptide antibiotic produced by Lactococcus lactis IO-1 (JCM 7638).	Matsusaki H, Chinachoti N, Sonomoto K, Ishizaki A.	Ann N Y Acad Sci	10.1111/j.1749-6632.1998.tb10351.x	1998
Metabolism	Lactococcin Q, a novel two-peptide bacteriocin produced by Lactococcus lactis QU 4.	Zendo T, Koga S, Shigeri Y, Nakayama J, Sonomoto K.	Appl Environ Microbiol	10.1128/aem.72.5.3383-3389.2006	2006
	Efficient production of polymer-grade L-lactic acid from corn stover hydrolyzate by thermophilic Bacillus sp. strain XZL4.	Xue Z, Wang L, Ju J, Yu B, Xu P, Ma Y.	Springerplus	10.1186/2193-1801-1-43	2012
Metabolism	Efficient homofermentative L-(+)-lactic acid production from xylose by a novel lactic acid bacterium, Enterococcus mundtii QU 25.	Abdel-Rahman MA, Tashiro Y, Zendo T, Hanada K, Shibata K, Sonomoto K.	Appl Environ Microbiol	10.1128/aem.02076-10	2011
Pathogenicity	Identification and characterization of lactocyclicin Q, a novel cyclic bacteriocin produced by Lactococcus sp. strain QU 12.	Sawa N, Zendo T, Kiyofuji J, Fujita K, Himeno K, Nakayama J, Sonomoto K.	Appl Environ Microbiol	10.1128/aem.02299-08	2009
Metabolism	Identification and characterization of leucocyclicin Q, a novel cyclic bacteriocin produced by Leuconostoc mesenteroides TK41401.	Masuda Y, Ono H, Kitagawa H, Ito H, Mu F, Sawa N, Zendo T, Sonomoto K.	Appl Environ Microbiol	10.1128/aem.06348-11	2011
Metabolism	Isolation and characterization of enterocin W, a novel two-peptide lantibiotic produced by Enterococcus faecalis NKR-4-1.	Sawa N, Wilaipun P, Kinoshita S, Zendo T, Leelawatcharamas V, Nakayama J, Sonomoto K.	Appl Environ Microbiol	10.1128/aem.06497-11	2012
Metabolism	Comparison of Different Lactobacilli Regarding Substrate Utilization and Their Tolerance Towards Lignocellulose Degradation Products.	Gubelt A, Blaschke L, Hahn T, Rupp S, Hirth T, Zibek S	Curr Microbiol	10.1007/s00284-020-02131-y	2020
Metabolism	Suppression of lactate production in fed-batch culture of some lactic acid bacteria with sucrose as the carbon source.	Kawai M, Tsuchiya A, Ishida J, Yoda N, Yashiki-Yamasaki S, Katakura Y	J Biosci Bioeng	10.1016/j.jbiosc.2019.11.009	2019
Genetics	Genomic features of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes xylose and produces high levels of L-lactic acid.	Shimizu-Kadota M, Kato H, Shiwa Y, Oshima K, Machii M, Araya-Kojima T, Zendo T, Hattori M, Sonomoto K, Yoshikawa H	Biosci Biotechnol Biochem	10.1271/bbb.130080	2013
Genetics	Complete genome sequence of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes xylose and produces high levels of L-lactic acid.	Kato H, Shiwa Y, Oshima K, Machii M, Araya-Kojima T, Zendo T, Shimizu-Kadota M, Hattori M, Sonomoto K, Yoshikawa H	J Bacteriol	10.1128/JB.00074-12	2012
Metabolism	Two different pathways for D-xylose metabolism and the effect of xylose concentration on the yield coefficient of L-lactate in mixed-acid fermentation by the lactic acid bacterium Lactococcus lactis IO-1.	Tanaka K, Komiyama A, Sonomoto K, Ishizaki A, Hall SJ, Stanbury PF	Appl Microbiol Biotechnol	10.1007/s00253-002-1078-5	2002

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#35548	Collection of Institut Pasteur ; Curators of the CIP; CIP 103449
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lactococcus lactis (CIP 103449, JCM 7638)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Compound production

Metabolite utilization

Metabolite production

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000344575

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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