Name: Lactococcus lactis CIP 103449
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 136206

Type strain:

Species: Lactococcus lactis

Culture col. no.: CIP 103449, JCM 7638

Strain history: CIP <- 1990, JCM <- strain O-1, Lactobacillus xylosus

NCBI tax ID(s): 1358 (species)

Other strains from Lactococcus lactis

L. lactis OJ, N30, DSM 20481, ATCC 19435, ... (type strain)
L. lactis Citrate plasmid, F7/2, DSM 4366
L. lactis 117, DSM 20175, ATCC 15577, CCUG ...
L. lactis 54296, DSM 20384
L. lactis 5, DSM 20250
L. lactis 2, DSM 20661, NCDO 176
L. lactis Berridge X 13, DSM 20729, ATCC ...
L. lactis E9, DSM 30863
L. lactis STI07582(IMET), 1136-53,
L. lactis STI07652(IMET), IMET 07690, Mer ...
L. lactis STI07667(IMET), M18,
L. lactis STI07670(IMET), RS/20,
L. lactis N34, RICE, CIP 70.57
L. lactis CIP A216
L. lactis H 214, N35, CIP 70.58
L. lactis J4 conserves, CIP A217
L. lactis J4 Alger, CIP A218
L. lactis 129.86, CIP 102103
L. lactis CCUG 21956, NCIMB 10493, ATCC 11007, ...
L. lactis CCUG 28474
L. lactis CCUG 31342
L. lactis CCUG 31972
L. lactis CCUG 32225
L. lactis CCUG 35127 B
L. lactis CCUG 38585
L. lactis CCUG 42927
L. lactis CCUG 44423
L. lactis CCUG 45692
L. lactis CCUG 45693
L. lactis CCUG 45695
L. lactis CCUG 47216
L. lactis CCUG 47618
L. lactis CCUG 52621
L. lactis CCUG 53296
L. lactis CCUG 58504
L. lactis CCUG 59643
L. lactis CCUG 60423
L. lactis CCUG 61221
L. lactis SV21, DSM 27303
L. lactis SB 17, DSM 111832
L. lactis SB 352, DSM 111868
L. lactis SB 150, DSM 111869
L. lactis SB 261, DSM 111870
L. lactis JCM 18008, FERM AP-21288
L. lactis CLA-AV-14, DSM 110082

Section

Lactococcus lactis CIP 103449 is a facultative anaerobe, mesophilic bacterium of the family Streptococcaceae.

facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Streptococcaceae*
	Genus	*Lactococcus*
	Species	**Lactococcus lactis**
	Full Scientific Name (LPSN)	Lactococcus lactis (Lister 1873) Schleifer et al. 1986

	Synonym	Streptococcus lactis
	Synonym	"Bacterium* lactis"*
	Synonym	Lactobacillus xylosus
	Synonym	Streptococcus lactis subsp. diacetilactis

Information on morphological and physiological properties Morphology

[Ref.: #35548]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #35548]	Cell shape	coccus-shaped

[Ref.: #35548]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %95.384

Information on culture and growth conditions Culture and growth conditions

[Ref.: #35548]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #35548]

Culture medium growth

[Ref.: #35548]

Culture medium composition

expand / minimize

[Ref.: #35548]

Culture medium

CIP Medium 40

[Ref.: #35548]

Culture medium growth

[Ref.: #35548]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #35548]			growth	37 °C
[Ref.: #67770]			growth	37 °C

[Ref.: #35548]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #35548]

Oxygen tolerance

facultative anaerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

[Ref.: #67770]

Name of produced compound

Nisin Z

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #35548]	nitrate ChEBI	-	reduction
[Ref.: #35548]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #35548]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #35548]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #35548]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #35548]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #35548]	9274	not determinedn.d.	+/-	-	-	-	+	+	-	-	-	+	+	+	+	-	-	-	-	+	-	+/-	+/-	+	+	+	+	+	+	+	+	-	+	+	-	-	-	+	-	-	+	-	-	-	-	-	-	-	+	-	+/-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #35548]	7542	-	-	-	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Water in the drain pit of a kitchen sink
[Ref.: #67770]	Geographic location (country and/or sea, region)	Fukuoka
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #35548]	Sample type/isolated from	Environment, Water in a drain pit of a kitchen sink
[Ref.: #35548]	Geographic location (country and/or sea, region)	Fukuoka
[Ref.: #35548]	Country	Japan
[Ref.: #35548]	Country ISO 3 Code	JPN
[Ref.: #35548]	Continent	Asia
[Ref.: #35548]	Isolation date	1986

[Ref.: #35548]	Geographic location (country and/or sea, region)	Fukuoaka-shi
[Ref.: #35548]	Country	Japan
[Ref.: #35548]	Country ISO 3 Code	JPN
[Ref.: #35548]	Continent	Asia
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence LC096207 (>99% sequence identity) for Lactococcus from Microbeatlas

Aquatic Samples	13841
Soil Samples	7135
Animal Samples	132033
Plant Samples	4677
Total Samples	157686

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #35548]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactococcus lactis subsp. lactis gene for 16S ribosomal RNA, partial sequence, strain: JCM 7638	LC096207	1486	ENA	1360 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactococcus lactis subsp. lactis IO-1	GCA_000344575	complete	GenBank	1046624 tax ID
[Ref.: #66792]	Lactococcus lactis subsp. lactis JCM 7638	GCA_001311315	contig	GenBank	1291762 tax ID	*
[Ref.: #66792]	Lactococcus lactis subsp. lactis JCM 7638	1291762.3	wgs	PATRIC	1291762 tax ID	*
[Ref.: #66792]	Lactococcus lactis lactis JCM 7638	2728369680	draft	JGI IMG/M	1291762 tax ID	*

[Ref.: #67770]

GC-content

38 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lactococcus lactis subsp. lactis JCM 7638 PATRIC: 1291762.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactococcus lactis subsp. lactis JCM 7638 PATRIC: 1291762.3
[Ref.: #69480]	motile	no	95.308	no
[Ref.: #69480]	gram-positive	yes	95.801	no
[Ref.: #69480]	anaerobic	no	96.264	no
[Ref.: #69480]	aerobic	no	96.959	no
[Ref.: #69480]	halophile	yes	64.831	no
[Ref.: #69480]	spore-forming	no	92.673	no
[Ref.: #69480]	glucose-util	yes	89.93	no
[Ref.: #69480]	flagellated	no	97.499	no
[Ref.: #69480]	thermophile	no	99.869	yes
[Ref.: #69480]	glucose-ferment	yes	87.117	no

Availability in culture collections External links

[Ref.: #35548]

Culture collection no.

CIP 103449, JCM 7638

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Comparison of Different Lactobacilli Regarding Substrate Utilization and Their Tolerance Towards Lignocellulose Degradation Products.	Gubelt A, Blaschke L, Hahn T, Rupp S, Hirth T, Zibek S	Curr Microbiol	10.1007/s00284-020-02131-y	2020	*
Metabolism	Suppression of lactate production in fed-batch culture of some lactic acid bacteria with sucrose as the carbon source.	Kawai M, Tsuchiya A, Ishida J, Yoda N, Yashiki-Yamasaki S, Katakura Y	J Biosci Bioeng	10.1016/j.jbiosc.2019.11.009	2019	*
Genetics	Genomic features of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes xylose and produces high levels of L-lactic acid.	Shimizu-Kadota M, Kato H, Shiwa Y, Oshima K, Machii M, Araya-Kojima T, Zendo T, Hattori M, Sonomoto K, Yoshikawa H	Biosci Biotechnol Biochem	10.1271/bbb.130080	2013	*
Genetics	Complete genome sequence of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes xylose and produces high levels of L-lactic acid.	Kato H, Shiwa Y, Oshima K, Machii M, Araya-Kojima T, Zendo T, Shimizu-Kadota M, Hattori M, Sonomoto K, Yoshikawa H	J Bacteriol	10.1128/JB.00074-12	2012	*
Metabolism	Two different pathways for D-xylose metabolism and the effect of xylose concentration on the yield coefficient of L-lactate in mixed-acid fermentation by the lactic acid bacterium Lactococcus lactis IO-1.	Tanaka K, Komiyama A, Sonomoto K, Ishizaki A, Hall SJ, Stanbury PF	Appl Microbiol Biotechnol	10.1007/s00253-002-1078-5	2002	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#35548 Collection of Institut Pasteur ; Curators of the CIP; CIP 103449

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Lactococcus lactis subsp. lactis JCM 7638

Lactococcus lactis subsp. lactis JCM 7638

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Change proposal