Lactococcus lactis (DSM 20661, NCDO 176, 2)

Name: Lactococcus lactis (DSM 20661, NCDO 176, 2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14703

Type strain

Strain Designation 2

Culture col. no. DSM 20661 NCDO 176 2

NCBI tax ID(s) 1358

Special Collections DSMZ

History DSMZ <- NCDO <- E. Pijanowski, 2

Links

Lactococcus lactis 2 is a bacterium of the family Streptococcaceae.

genome sequence Bacteria

Name and taxonomic classification

SI-ID 3418

LMG 7949,NCFB 176,NCDO 176,NCIMB 700176,CECT 4431,CCT 0330,DSM 20661

@ref 20215

Last LPSN update 2026-05-29 08:28:21

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Streptococcaceae

Genus Lactococcus

Species Lactococcus lactis

Full scientific name Lactococcus lactis (Lister 1873) Schleifer et al. 1986

Synonyms (4)

Lactobacillus xylosus
"Bacterium lactis"
Streptococcus lactis
Streptococcus lactis subsp. diacetilactis

BacDive ID	Other strains from **Lactococcus lactis** (42)	Type strain
14702	L. lactis OJ, N30, DSM 20481, ATCC 19435, CCM 1877, ... (type strain)
14694	L. lactis Citrate plasmid, F7/2, DSM 4366
14699	L. lactis 117, DSM 20175, ATCC 15577, CCUG 21962, NRRL ...
14700	L. lactis 54296, DSM 20384
14701	L. lactis 5, DSM 20250
14704	L. lactis Berridge X 13, DSM 20729, ATCC 11454, CCM ...
14705	L. lactis E9, DSM 30863
134825	L. lactis N34, RICE, CIP 70.57
134851	L. lactis CIP A216
135401	L. lactis H 214, N35, CIP 70.58
136206	L. lactis CIP 103449, JCM 7638
138955	L. lactis J4 conserves, CIP A217
138966	L. lactis J4 Alger, CIP A218
139482	L. lactis 129.86, CIP 102103
143933	L. lactis CCUG 21956, NCIMB 10493, ATCC 11007, LMG ...
145429	L. lactis CCUG 28474
146507	L. lactis CCUG 31342
146700	L. lactis CCUG 31972
146846	L. lactis CCUG 32225
148267	L. lactis CCUG 35127 B
149871	L. lactis CCUG 38585
150979	L. lactis CCUG 42927
151600	L. lactis CCUG 44423
152071	L. lactis CCUG 45692
152072	L. lactis CCUG 45693
152073	L. lactis CCUG 45695
152621	L. lactis CCUG 47216
152790	L. lactis CCUG 47618
154162	L. lactis CCUG 52621
154377	L. lactis CCUG 53296
155625	L. lactis CCUG 58504
155919	L. lactis CCUG 59643
156113	L. lactis CCUG 60423
156342	L. lactis CCUG 61221
160084	L. lactis SV21, DSM 27303
160085	L. lactis SB 17, DSM 111832
160086	L. lactis SB 352, DSM 111868
160087	L. lactis SB 150, DSM 111869
160088	L. lactis SB 261, DSM 111870
162165	L. lactis JCM 18008, FERM AP-21288
169254	L. lactis CLA-AV-14, DSM 110082
174486	L. lactis A537/21, DSM 117780

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125438		90.5
125438		93.175	positive

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
8881	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)		Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
8881	positive	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	95.579

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	+	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
8881	-	-	-	-	-	+	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	-	+	+	+	+	+	+	-	-	+	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Pathogenicity animal	Biosafety level	Biosafety level comment
8881	yes, in single cases	yes, in single cases	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
124043	ASM1602881v1 assembly for Lactococcus lactis FDAARGOS_866	complete	GCA_016028815	1358.1329	1358	97.92
66792	ASM966391v1 assembly for Lactococcus lactis subsp. lactis NCDO176	contig	GCA_009663915	1360.552	1360	54.79

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1602881v1 assembly for Lactococcus lactis FDAARGOS_866
BV-BRC: 1358.1329

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	gram_stain	BacteriaNetⓘ	positive	51.93	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	95.58	no
125439	motility	BacteriaNetⓘ	yes	43.00	no
125439	spore_formation	BacteriaNetⓘ	no	76.78	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactococcus lactis strain FDAARGOS_866 strain Not applicable
PATRIC: 1358.1329

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	93.18	no
125438	anaerobic	anaerobicⓘ	no	91.19	no
125438	spore-forming	spore-formingⓘ	no	83.58	no
125438	aerobic	aerobicⓘ	no	95.86	no
125438	thermophilic	thermophileⓘ	no	98.50	yes
125438	flagellated	motile2+ⓘ	no	90.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Identification of Species and Subspecies of Lactic Acid Bacteria Present in Spanish Cheeses Type "Torta" by MALDI-TOF MS and pheS gene Analyses.	Sanchez-Juanes F, Teixeira-Martin V, Gonzalez-Buitrago JM, Velazquez E, Flores-Felix JD.	Microorganisms	10.3390/microorganisms8020301	2020
	Identification of lactococci and enterococci by colony hybridization with 23S rRNA-targeted oligonucleotide probes.	Betzl D, Ludwig W, Schleifer KH.	Appl Environ Microbiol	10.1128/aem.56.9.2927-2929.1990	1990
	Characterization and antimicrobial activity of Pediococcus species isolated from South African farm-style cheese	Gurira OZ, Buys EM.	Food Microbiol	10.1016/j.fm.2004.08.001	2005
	Effect of the lactoperoxidase system on the activity of mesophilic cheese starter cultures in goat milk	Seifu E, Buys EM, Donkin EF.	Int Dairy J	10.1016/s0958-6946(03)00122-5	2003
Enzymology	Plasmid transformation by electroporation of Leuconostoc paramesenteroides and its use in molecular cloning.	David S, Simons G, De Vos WM.	Appl Environ Microbiol	10.1128/aem.55.6.1483-1489.1989	1989
Enzymology	Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris: isolation and characterization of the protein and its gene.	Holo H, Nilssen O, Nes IF.	J Bacteriol	10.1128/jb.173.12.3879-3887.1991	1991
	Autolysis of lactococci: detection of lytic enzymes by polyacrylamide gel electrophoresis and characterization in buffer systems.	Ostlie HM, Vegarud G, Langsrud T.	Appl Environ Microbiol	10.1128/aem.61.10.3598-3603.1995	1995
	Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing.	Gasson MJ.	J Bacteriol	10.1128/jb.154.1.1-9.1983	1983
Enzymology	Characterization of plasmid-encoded citrate permease (citP) genes from Leuconostoc species reveals high sequence conservation with the Lactococcus lactis citP gene.	Vaughan EE, David S, Harrington A, Daly C, Fitzgerald GF, De Vos WM	Appl Environ Microbiol	10.1128/aem.61.8.3172-3176.1995	1995
Enzymology	Nucleotide sequence and expression in Escherichia coli of the Lactococcus lactis citrate permease gene.	David S, van der Rest ME, Driessen AJ, Simons G, de Vos WM	J Bacteriol	10.1128/jb.172.10.5789-5794.1990	1990

References

#8881	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20661
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lactococcus lactis (DSM 20661, NCDO 176, 2)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

API 50CHac

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_016028815

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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