Name: Bullifex porci NM-380-WT-3C1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 159029

Type strain:

Species: Bullifex porci

Strain Designation: NM-380-WT-3C1

Culture col. no.: DSM 105750, JCM 34393

Strain history: B. Abt; DSMZ, Germany; DSM 105750 (=NM-380-WT-3C1) <-- T. Clavel; RWTH Aachen, Germany.

NCBI tax ID(s): 2606638 (species)

SI-ID 408066

DSM 105750

Special Collections

Section

Bullifex porci NM-380-WT-3C1 is an anaerobe, coccus-shaped bacterium that was isolated from pig, WT, 20 months old, feces.

coccus-shaped
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Spirochaetota*
	Class	*Spirochaetia*
	Order	*Spirochaetales*
	Family	*Spirochaetaceae*
	Genus	*Bullifex*
	Species	**Bullifex porci**
	Full Scientific Name (LPSN)	Bullifex porci Wylensek et al. 2021

Information on morphological and physiological properties Morphology

[Ref.: #69645]	Cell length	1-10 µm

[Ref.: #69645]	Cell shape	coccus-shaped

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65852]

Culture medium

WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)

[Ref.: #65852]

Culture medium growth

[Ref.: #65852]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65852]

Culture medium composition

expand / minimize

[Ref.: #69645]

Culture medium

M9 minimal salts

[Ref.: #69645]

Culture medium growth

[Ref.: #69645]

Culture medium composition

expand / minimize

[Ref.: #69645]

Culture medium

Brain Heart Infusion (BHI) agar

[Ref.: #69645]

Culture medium growth

[Ref.: #69645]

Culture medium composition

expand / minimize

[Ref.: #69645]

Culture medium

Wilkins-Chalgren Anaerobe broth

[Ref.: #69645]

Culture medium growth

[Ref.: #69645]

Culture medium composition

expand / minimize

[Ref.: #69645]

Culture medium

Brain Heart Infusion (BHI) agar

[Ref.: #69645]

Culture medium growth

[Ref.: #69645]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #65852]	growth	37 °C
[Ref.: #67770]	growth	37 °C
[Ref.: #69645]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69645]

Oxygen tolerance

anaerobe

[Ref.: #69645]

Observation

After several days of growth, they tend to form infiated round cells with an opaque, half-moon-shaped compartment on one side of the cell that stains positive with DAPI

	Antibiotica	Metabolite	Antibiotic resistance	Concentration
[Ref.: #69645]		ampicillin ChEBI	yes	100	Unit (disc)
[Ref.: #69645]		penicillin ChEBI	yes	100	Unit (disc)

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65852]	Sample type/isolated from	pig, WT, 20 months old, feces
[Ref.: #65852]	Geographic location (country and/or sea, region)	Kranzberg (Bavaria)
[Ref.: #65852]	Country	Germany
[Ref.: #65852]	Country ISO 3 Code	DEU
[Ref.: #65852]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Feces of a German Landrace pig
[Ref.: #67770]	Geographic location (country and/or sea, region)	Freising
[Ref.: #67770]	Country	Germany
[Ref.: #67770]	Country ISO 3 Code	DEU
[Ref.: #67770]	Continent	Europe

[Ref.: #69645]	Sample type/isolated from	Pig feces
[Ref.: #69645]	Geographic location (country and/or sea, region)	Thalhausen, Bavaria
[Ref.: #69645]	Country	Germany
[Ref.: #69645]	Country ISO 3 Code	DEU
[Ref.: #69645]	Continent	Europe
[Ref.: #69645]	Enrichment culture	M9 minimal salts
[Ref.: #69645]	Enrichment culture duration	5-20 days
[Ref.: #69645]	Enrichment culture temperature	37 ̊C
[Ref.: #69645]	Isolation/enrichment procedure	anaerobic
* marker position based on {}

Isolation sources categories

#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Mammals	#Suidae (Pig,Swine)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence MN537494 (>99% sequence identity) for Spirochaetaceae from Microbeatlas

Aquatic Samples	361
Soil Samples	298
Animal Samples	16394
Plant Samples	166
Total Samples	17219

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65852]	Bullifex porci strain NM-380-WT-3C1 16S ribosomal RNA gene, partial sequence	MN537494	1499	GenBank ENA	2652310 tax ID

[Ref.: #6585289]

Protologger flat file download

Protologger is an all-in-one genome description tool, aimed at simplifying the process of gathering the data required for writing protologues. This includes providing; taxonomic, functional and ecological insights. Protologger is freely accessible at www.protologger.de

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #69645]	Whole genome shotgun	VUNN00000000		GenBank ENA	2606638 tax ID
[Ref.: #66792]	Bullifex porci NM-380-WT-3C1	GCA_009695635	contig	GenBank ENA	2606638 tax ID	*
[Ref.: #66792]	Spirochaetales bacterium NM-380-WT-3C1	2606638.3	wgs	PATRIC	2606638 tax ID	*

[Ref.: #67770]

GC-content

35.9 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Spirochaetales bacterium NM-380-WT-3C1 PATRIC: 2606638.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	58	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Spirochaetales bacterium NM-380-WT-3C1 PATRIC: 2606638.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	80.803	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	86.254	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	83.331	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	96.932	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	86.624	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	84.625	no

Availability in culture collections External links

[Ref.: #65852]

Culture collection no.

DSM 105750, JCM 34393

[Ref.: #111543]

SI-ID 408066

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65852

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105750

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#69645

David Wylensek, Thomas C. A. Hitch, Thomas Riedel, Afrizal Afrizal, Neeraj Kumar, Esther Wortmann, Tianzhe Liu, Saravanan Devendran, Till R. Lesker, Sara B. Hernández, Viktoria Heine, Eva M. Buhl, Paul M. D'Agostino, Fabio Cumbo, Thomas Fischöder, Marzena Wyschkon, Torey Looft, Valeria R. Parreira, Birte Abt, Heidi L. Doden, Lindsey Ly, João M. P. Alves, Markus Reichlin, Krzysztof Flisikowski, Laura Navarro Suarez, Anthony P. Neumann, Garret Suen, Tomas de Wouters, Sascha Rohn, Ilias Lagkouvardos, Emma Allen-Vercoe, Cathrin Spröer, Boyke Bunk, Anja J. Taverne-Thiele, Marcel Giesbers, Jerry M. Wells, Klaus Neuhaus, Angelika Schnieke, Felipe Cava, Nicola Segata, Lothar Elling, Till Strowig, Jason M. Ridlon, Tobias A. M. Gulder, Jörg Overmann, Thomas Clavel: A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity. Nat. Commun. 11: 2020 ( DOI 10.1038/s41467-020-19929-w )

#111543

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID408066.1 )

* These data were automatically processed and therefore are not curated

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Spirochaetales bacterium NM-380-WT-3C1

Spirochaetales bacterium NM-380-WT-3C1

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