Strain identifier
BacDive ID: 159029
Type strain: 
Species: Bullifex porci
Strain Designation: NM-380-WT-3C1
Strain history: B. Abt; DSMZ, Germany; DSM 105750 (=NM-380-WT-3C1) <-- T. Clavel; RWTH Aachen, Germany.
NCBI tax ID(s): 2606638 (species)
version 9.3 (current version)
General
@ref: 65852
BacDive-ID: 159029
DSM-Number: 105750
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, coccus-shaped
description: Bullifex porci NM-380-WT-3C1 is an anaerobe, mesophilic, coccus-shaped bacterium that was isolated from pig, WT, 20 months old, feces.
NCBI tax id
- NCBI tax id: 2606638
- Matching level: species
strain history
| @ref | history |
|---|---|
| 65852 | <- T. Clavel , TU Munich, Freising-Weihenstephan, Germany; NM-380-WT-3C1 |
| 67770 | B. Abt; DSMZ, Germany; DSM 105750 (=NM-380-WT-3C1) <-- T. Clavel; RWTH Aachen, Germany. |
doi: 10.13145/bacdive159029.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/spirochaetota
- domain: Bacteria
- phylum: Spirochaetota
- class: Spirochaetia
- order: Spirochaetales
- family: Spirochaetaceae
- genus: Bullifex
- species: Bullifex porci
- full scientific name: Bullifex porci Wylensek et al. 2021
@ref: 65852
domain: Bacteria
phylum: Spirochaetes
class: Spirochaetia
order: Spirochaetales
family: Spirochaetaceae
genus: Bullifex
species: Bullifex porci
full scientific name: Bullifex porci
strain designation: NM-380-WT-3C1
type strain: yes
Morphology
cell morphology
| @ref | gram stain | confidence | cell length | cell shape |
|---|---|---|---|---|
| 125439 | negative | 98.4 | ||
| 69645 | 1-10 µm | coccus-shaped |
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 65852 | WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) | yes | https://mediadive.dsmz.de/medium/339 | Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water |
| 69645 | M9 minimal salts | yes | (Merck, ref. M6030) | |
| 69645 | Brain Heart Infusion (BHI) agar | yes | supplemented with rumen fluid (10%) | |
| 69645 | Wilkins-Chalgren Anaerobe broth | yes | supplemented with L-cysteine and DTT (0.05% and 0.02% (w/v), respectively) | |
| 69645 | Brain Heart Infusion (BHI) agar | yes | supplemented with 10% rumen ?uid |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 65852 | positive | growth | 37 |
| 67770 | positive | growth | 37 |
| 69645 | positive | growth | 37 |
Physiology and metabolism
oxygen tolerance
- @ref: 69645
- oxygen tolerance: anaerobe
observation
- @ref: 69645
- observation: After several days of growth, they tend to form infiated round cells with an opaque, half-moon-shaped compartment on one side of the cell that stains positive with DAPI
antibiotic resistance
| @ref | ChEBI | metabolite | is antibiotic | is resistant | resistance conc. |
|---|---|---|---|---|---|
| 69645 | 28971 | ampicillin | yes | yes | 100 Unit (disc) |
| 69645 | 17334 | penicillin | yes | yes | 100 Unit (disc) |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | enrichment culture | enrichment culture duration | enrichment culture temperature | isolation procedure |
|---|---|---|---|---|---|---|---|---|---|
| 65852 | pig, WT, 20 months old, feces | Kranzberg (Bavaria) | Germany | DEU | Europe | ||||
| 67770 | Feces of a German Landrace pig | Freising | Germany | DEU | Europe | ||||
| 69645 | Pig feces | Thalhausen, Bavaria | Germany | DEU | Europe | M9 minimal salts | 5-20 days | 37 | anaerobic |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
| #Host | #Mammals | #Suidae (Pig,Swine) |
taxonmaps
- @ref: 69479
- File name: preview.99_68749.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6715;96_29586;97_37332;98_48370;99_68749&stattab=map
- Last taxonomy: Spirochaetaceae
- 16S sequence: MN537494
- Sequence Identity:
- Total samples: 17219
- soil counts: 298
- aquatic counts: 361
- animal counts: 16394
- plant counts: 166
Sequence information
16S sequences
- @ref: 65852
- description: Bullifex porci strain NM-380-WT-3C1 16S ribosomal RNA gene, partial sequence
- accession: MN537494
- length: 1499
- database: nuccore
- NCBI tax ID: 2652310
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Bullifex porci NM-380-WT-3C1 | GCA_009695635 | contig | ncbi | 2606638 |
| 66792 | Spirochaetales bacterium NM-380-WT-3C1 | 2606638.3 | wgs | patric | 2606638 |
| 69645 | Whole genome shotgun | VUNN00000000 | ncbi | 2606638 |
GC content
- @ref: 67770
- GC-content: 35.9
- method: genome sequence analysis
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 80.803 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | yes | 86.254 | no |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 83.331 | no |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 96.932 | yes |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | no | 86.624 | yes |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | no | 84.625 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | no | 89.6 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | no | 59.9 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | negative | 98.4 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | obligate aerobe | 88.4 |
External links
@ref: 65852
culture collection no.: DSM 105750, JCM 34393
straininfo link
- @ref: 111543
- straininfo: 408066
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 65852 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-105750 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105750) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69645 | David Wylensek, Thomas C. A. Hitch, Thomas Riedel, Afrizal Afrizal, Neeraj Kumar, Esther Wortmann, Tianzhe Liu, Saravanan Devendran, Till R. Lesker, Sara B. Hernández, Viktoria Heine, Eva M. Buhl, Paul M. D'Agostino, Fabio Cumbo, Thomas Fischöder, Marzena Wyschkon, Torey Looft, Valeria R. Parreira, Birte Abt, Heidi L. Doden, Lindsey Ly, João M. P. Alves, Markus Reichlin, Krzysztof Flisikowski, Laura Navarro Suarez, Anthony P. Neumann, Garret Suen, Tomas de Wouters, Sascha Rohn, Ilias Lagkouvardos, Emma Allen-Vercoe, Cathrin Spröer, Boyke Bunk, Anja J. Taverne-Thiele, Marcel Giesbers, Jerry M. Wells, Klaus Neuhaus, Angelika Schnieke, Felipe Cava, Nicola Segata, Lothar Elling, Till Strowig, Jason M. Ridlon, Tobias A. M. Gulder, Jörg Overmann, Thomas Clavel | A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity | 10.1038/s41467-020-19929-w | |
| 111543 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID408066.1 | |
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | 10.1101/2024.08.12.607695 | |
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 | https://github.com/GenomeNet/deepG |