Strain identifier

BacDive ID: 159029

Type strain: Yes

Species: Bullifex porci

Strain Designation: NM-380-WT-3C1

Strain history: B. Abt; DSMZ, Germany; DSM 105750 (=NM-380-WT-3C1) <-- T. Clavel; RWTH Aachen, Germany.

NCBI tax ID(s): 2606638 (species)

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General

@ref: 65852

BacDive-ID: 159029

DSM-Number: 105750

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative

description: Bullifex porci NM-380-WT-3C1 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from pig, WT, 20 months old, feces.

NCBI tax id

  • NCBI tax id: 2606638
  • Matching level: species

strain history

@refhistory
65852<- T. Clavel , TU Munich, Freising-Weihenstephan, Germany; NM-380-WT-3C1
67770B. Abt; DSMZ, Germany; DSM 105750 (=NM-380-WT-3C1) <-- T. Clavel; RWTH Aachen, Germany.

doi: 10.13145/bacdive159029.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/spirochaetota
  • domain: Bacteria
  • phylum: Spirochaetota
  • class: Spirochaetia
  • order: Spirochaetales
  • family: Spirochaetaceae
  • genus: Bullifex
  • species: Bullifex porci
  • full scientific name: Bullifex porci Wylensek et al. 2021

@ref: 65852

domain: Bacteria

phylum: Spirochaetes

class: Spirochaetia

order: Spirochaetales

family: Spirochaetaceae

genus: Bullifex

species: Bullifex porci

full scientific name: Bullifex porci

strain designation: NM-380-WT-3C1

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell lengthcell shape
69480negative93.356
696451-10 µmcoccus-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65852WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)yeshttps://mediadive.dsmz.de/medium/339Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water
69645M9 minimal saltsyes(Merck, ref. M6030)
69645Brain Heart Infusion (BHI) agaryessupplemented with rumen fluid (10%)
69645Wilkins-Chalgren Anaerobe brothyessupplemented with L-cysteine and DTT (0.05% and 0.02% (w/v), respectively)
69645Brain Heart Infusion (BHI) agaryessupplemented with 10% rumen ?uid

culture temp

@refgrowthtypetemperaturerange
65852positivegrowth37mesophilic
67770positivegrowth37mesophilic
69645positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
69480anaerobe98.698
69645anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.997

observation

  • @ref: 69645
  • observation: After several days of growth, they tend to form infiated round cells with an opaque, half-moon-shaped compartment on one side of the cell that stains positive with DAPI

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.
6964528971ampicillinyesyes100 Unit (disc)
6964517334penicillinyesyes100 Unit (disc)

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
65852pig, WT, 20 months old, fecesKranzberg (Bavaria)GermanyDEUEurope
67770Feces of a German Landrace pigFreisingGermanyDEUEurope
69645Pig fecesThalhausen, BavariaGermanyDEUEuropeM9 minimal salts5-20 days37anaerobic

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Mammals#Suidae (Pig,Swine)

taxonmaps

  • @ref: 69479
  • File name: preview.99_68749.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6715;96_29586;97_37332;98_48370;99_68749&stattab=map
  • Last taxonomy: Spirochaetaceae
  • 16S sequence: MN537494
  • Sequence Identity:
  • Total samples: 17219
  • soil counts: 298
  • aquatic counts: 361
  • animal counts: 16394
  • plant counts: 166

Sequence information

16S sequences

  • @ref: 65852
  • description: Spirochaetaceae bacterium DSM 105750 16S ribosomal RNA gene, partial sequence
  • accession: MN537494
  • length: 1499
  • database: ena
  • NCBI tax ID: 2652310

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bullifex porci NM-380-WT-3C1GCA_009695635contigncbi2606638
66792Spirochaetales bacterium NM-380-WT-3C12606638.3wgspatric2606638
69645Whole genome shotgunVUNN00000000ncbi2606638

GC content

  • @ref: 67770
  • GC-content: 35.9
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes58no
motileno87.864no
gram-positiveno81.696no
anaerobicyes98.874no
aerobicno98.057no
halophileno73.608no
spore-formingno96.167no
glucose-utilyes87.428no
flagellatedno97.584no
thermophileno97.86yes
glucose-fermentyes64.995no

External links

@ref: 65852

culture collection no.: DSM 105750, JCM 34393

straininfo link

  • @ref: 111543
  • straininfo: 408066

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65852Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-105750Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105750)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69645David Wylensek, Thomas C. A. Hitch, Thomas Riedel, Afrizal Afrizal, Neeraj Kumar, Esther Wortmann, Tianzhe Liu, Saravanan Devendran, Till R. Lesker, Sara B. Hernández, Viktoria Heine, Eva M. Buhl, Paul M. D'Agostino, Fabio Cumbo, Thomas Fischöder, Marzena Wyschkon, Torey Looft, Valeria R. Parreira, Birte Abt, Heidi L. Doden, Lindsey Ly, João M. P. Alves, Markus Reichlin, Krzysztof Flisikowski, Laura Navarro Suarez, Anthony P. Neumann, Garret Suen, Tomas de Wouters, Sascha Rohn, Ilias Lagkouvardos, Emma Allen-Vercoe, Cathrin Spröer, Boyke Bunk, Anja J. Taverne-Thiele, Marcel Giesbers, Jerry M. Wells, Klaus Neuhaus, Angelika Schnieke, Felipe Cava, Nicola Segata, Lothar Elling, Till Strowig, Jason M. Ridlon, Tobias A. M. Gulder, Jörg Overmann, Thomas ClavelA collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity10.1038/s41467-020-19929-w
111543Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID408066.1