Archive BacDiveID:10.13145/bacdive159029.20240916.9.1

Strain identifier

BacDive ID: 159029

Type strain:

Species: Bullifex porci

Strain Designation: NM-380-WT-3C1

Strain history: B. Abt; DSMZ, Germany; DSM 105750 (=NM-380-WT-3C1) <-- T. Clavel; RWTH Aachen, Germany.

NCBI tax ID(s): 2606638 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 65852

BacDive-ID: 159029

DSM-Number: 105750

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, coccus-shaped

description: Bullifex porci NM-380-WT-3C1 is an anaerobe, coccus-shaped bacterium that was isolated from pig, WT, 20 months old, feces.

NCBI tax id

NCBI tax id: 2606638
Matching level: species

strain history

@ref	history
65852	<- T. Clavel , TU Munich, Freising-Weihenstephan, Germany; NM-380-WT-3C1
67770	B. Abt; DSMZ, Germany; DSM 105750 (=NM-380-WT-3C1) <-- T. Clavel; RWTH Aachen, Germany.

doi: 10.13145/bacdive159029.20240916.9.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/spirochaetota
domain: Bacteria
phylum: Spirochaetota
class: Spirochaetia
order: Spirochaetales
family: Spirochaetaceae
genus: Bullifex
species: Bullifex porci
full scientific name: Bullifex porci Wylensek et al. 2021

@ref: 65852

domain: Bacteria

phylum: Spirochaetes

class: Spirochaetia

order: Spirochaetales

family: Spirochaetaceae

genus: Bullifex

species: Bullifex porci

full scientific name: Bullifex porci

strain designation: NM-380-WT-3C1

type strain: yes

Morphology

cell morphology

@ref: 69645
cell length: 1-10 µm
cell shape: coccus-shaped

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
65852	WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)	yes	https://mediadive.dsmz.de/medium/339	Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water
69645	M9 minimal salts	yes		(Merck, ref. M6030)
69645	Brain Heart Infusion (BHI) agar	yes		supplemented with rumen fluid (10%)
69645	Wilkins-Chalgren Anaerobe broth	yes		supplemented with L-cysteine and DTT (0.05% and 0.02% (w/v), respectively)
69645	Brain Heart Infusion (BHI) agar	yes		supplemented with 10% rumen ?uid

culture temp

@ref	growth	type	temperature
65852	positive	growth	37
67770	positive	growth	37
69645	positive	growth	37

Physiology and metabolism

oxygen tolerance

@ref: 69645
oxygen tolerance: anaerobe

observation

@ref: 69645
observation: After several days of growth, they tend to form infiated round cells with an opaque, half-moon-shaped compartment on one side of the cell that stains positive with DAPI

antibiotic resistance

@ref	ChEBI	metabolite	is antibiotic	is resistant	resistance conc.
69645	28971	ampicillin	yes	yes	100 Unit (disc)
69645	17334	penicillin	yes	yes	100 Unit (disc)

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent	enrichment culture	enrichment culture duration	enrichment culture temperature	isolation procedure
65852	pig, WT, 20 months old, feces	Kranzberg (Bavaria)	Germany	DEU	Europe
67770	Feces of a German Landrace pig	Freising	Germany	DEU	Europe
69645	Pig feces	Thalhausen, Bavaria	Germany	DEU	Europe	M9 minimal salts	5-20 days	37	anaerobic

isolation source categories

Cat1	Cat2	Cat3
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Mammals	#Suidae (Pig,Swine)

taxonmaps

@ref: 69479
File name: preview.99_68749.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6715;96_29586;97_37332;98_48370;99_68749&stattab=map
Last taxonomy: Spirochaetaceae
16S sequence: MN537494
Sequence Identity:
Total samples: 17219
soil counts: 298
aquatic counts: 361
animal counts: 16394
plant counts: 166

Sequence information

16S sequences

@ref: 65852
description: Bullifex porci strain NM-380-WT-3C1 16S ribosomal RNA gene, partial sequence
accession: MN537494
length: 1499
database: nuccore
NCBI tax ID: 2652310

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Bullifex porci NM-380-WT-3C1	GCA_009695635	contig	ncbi	2606638
66792	Spirochaetales bacterium NM-380-WT-3C1	2606638.3	wgs	patric	2606638
69645	Whole genome shotgun	VUNN00000000		ncbi	2606638

GC content

@ref: 67770
GC-content: 35.9
method: genome sequence analysis

Genome-based predictions

predictions

@ref	trait	model	description	prediction	confidence	training_data
69481	spore-forming	spore-forming	Ability to form endo- or exospores	yes	58	no
69480	gram-positive	gram-positive	Positive reaction to Gram-staining	no	80.803	no
69480	anaerobic	anaerobic	Ability to grow under anoxygenic conditions (including facultative anaerobes)	yes	86.254	no
69480	spore-forming	spore-forming	Ability to form endo- or exospores	no	83.331	no
69480	aerobic	aerobic	Ability to grow under oxygenic conditions (including facultative aerobes)	no	96.932	yes
69480	thermophilic	thermophile	Ability to grow at temperatures above or equal to 45°C	no	86.624	yes
69480	flagellated	motile2+	Ability to perform flagellated movement	no	84.625	no

External links

@ref: 65852

culture collection no.: DSM 105750, JCM 34393

straininfo link

@ref: 111543
straininfo: 408066

Reference

@id	authors	title	doi/url	catalogue
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ	10.1099/ijsem.0.004332
65852	Curators of the DSMZ		https://www.dsmz.de/collection/catalogue/details/culture/DSM-105750	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105750)
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)	https://diaspora-project.de/progress.html#genomes
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.	MicrobeAtlas 1.0 beta	https://microbeatlas.org/
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)	https://diaspora-project.de/progress.html#genomes
69481	Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch	Predictions based on the model GenomeNet Sporulation v. 1	10.21203/rs.3.rs-2527258/v1
69645	David Wylensek, Thomas C. A. Hitch, Thomas Riedel, Afrizal Afrizal, Neeraj Kumar, Esther Wortmann, Tianzhe Liu, Saravanan Devendran, Till R. Lesker, Sara B. Hernández, Viktoria Heine, Eva M. Buhl, Paul M. D'Agostino, Fabio Cumbo, Thomas Fischöder, Marzena Wyschkon, Torey Looft, Valeria R. Parreira, Birte Abt, Heidi L. Doden, Lindsey Ly, João M. P. Alves, Markus Reichlin, Krzysztof Flisikowski, Laura Navarro Suarez, Anthony P. Neumann, Garret Suen, Tomas de Wouters, Sascha Rohn, Ilias Lagkouvardos, Emma Allen-Vercoe, Cathrin Spröer, Boyke Bunk, Anja J. Taverne-Thiele, Marcel Giesbers, Jerry M. Wells, Klaus Neuhaus, Angelika Schnieke, Felipe Cava, Nicola Segata, Lothar Elling, Till Strowig, Jason M. Ridlon, Tobias A. M. Gulder, Jörg Overmann, Thomas Clavel	A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity	10.1038/s41467-020-19929-w
111543	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.	StrainInfo: A central database for resolving microbial strain identifiers	10.60712/SI-ID408066.1