Name: Sporolactobacillus laevolacticus M-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14387

Type strain:

Species: Sporolactobacillus laevolacticus

Strain Designation: M-1

Culture col. no.: DSM 2310, ATCC 23493, NCIB 10270, JCM 2514, IAM 12322, NCIMB 10270, CIP 104422

Strain history: CIP <- 1995, JCM <- IAM <- O. Nakamaya: strain M-1

NCBI tax ID(s): 33018 (species)

SI-ID 46618

ATCC 23493, CIP 104422, DSM 2310, IAM 12322, JCM 2514, NCIMB 10270, NCIB 10270

Other strains from Sporolactobacillus laevolacticus

Section

Sporolactobacillus laevolacticus M-1 is a facultative anaerobe, spore-forming, mesophilic bacterium that produces lactate and was isolated from rhizosphere of Trifolium repens.

lactate production
Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Sporolactobacillaceae*
	Genus	*Sporolactobacillus*
	Species	**Sporolactobacillus laevolacticus**
	Full Scientific Name (LPSN)	Sporolactobacillus laevolacticus (Andersch et al. 1994 ex Nakayama and Yanoshi 1967) Hatayama et al. 2006

Synonym

Bacillus laevolacticus

Information on morphological and physiological properties Morphology

[Ref.: #120547]	Gram stain	positive

[Ref.: #120547]	Cell shape	rod-shaped

[Ref.: #120547]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #37933]

Culture medium

MEDIUM 41- for Lactobacillus, Leuconostoc, Weissella, Pediococcus, Sporolactobacillus inulinus

[Ref.: #37933]

Culture medium growth

[Ref.: #37933]

Culture medium composition

expand / minimize

[Ref.: #1038]

Culture medium

BACILLUS "RACEMILACTICUS" MEDIUM (DSMZ Medium 8)

[Ref.: #1038]

Culture medium growth

[Ref.: #1038]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1038]

Culture medium composition

expand / minimize

[Ref.: #1038]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #1038]

Culture medium growth

[Ref.: #1038]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1038]

Culture medium composition

expand / minimize

[Ref.: #120547]

Culture medium

CIP Medium 41

[Ref.: #120547]

Culture medium growth

[Ref.: #120547]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #1038]		growth	30 °C
[Ref.: #37933]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #120547]		growth	30-37 °C
[Ref.: #120547]	no	growth	10 °C
[Ref.: #120547]	no	growth	15 °C
[Ref.: #120547]	no	growth	45 °C
[Ref.: #120547]	no	growth	55 °C

[Ref.: #1038]	Temperature range	mesophilic
[Ref.: #37933]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #120547]	Temperature range	mesophilic
[Ref.: #120547]	Temperature range	psychrophilic
[Ref.: #120547]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #120547]

Oxygen tolerance

facultative anaerobe

[Ref.: #120547]

Ability of spore formation

yes

[Ref.: #1038]

Name of produced compound

D(-) lactic acid

	Salt	Tested relation		Salt conc.
[Ref.: #120547]	NaCl		growth	0-2	%
[Ref.: #120547]	NaCl	no	growth	4	%
[Ref.: #120547]	NaCl	no	growth	6	%
[Ref.: #120547]	NaCl	no	growth	8	%
[Ref.: #120547]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #120547]	citrate ChEBI	-	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #120547]	nitrate ChEBI	-	reduction
[Ref.: #120547]	nitrate ChEBI	+	respiration
[Ref.: #120547]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120547]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120547]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120547]	amylase	+
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120547]	caseinase	+	3.4.21.50
[Ref.: #120547]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #120547]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #120547]	gelatinase	-
[Ref.: #120547]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120547]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120547]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120547]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120547]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #120547]	9600	not determinedn.d.	-	-	-	-	-	-	-	-	-	+	+	+	+	+	-	-	-	+	-	-	-	+	-	-	-	-	-	+	+/-	+	+	-	+	+	+	+/-	+/-	-	-	+	-	+	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120547]	7940	-	+	+	-	-	-	-	-	-	-	+	+	+	+	-	-	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1038]	Sample type/isolated from	rhizosphere of Trifolium repens
[Ref.: #1038]	Host species	Trifolium repens
[Ref.: #1038]	Geographic location (country and/or sea, region)	Kofu
[Ref.: #1038]	Country	Japan
[Ref.: #1038]	Country ISO 3 Code	JPN
[Ref.: #1038]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Rhizosphere of clover (Trifolium repens)
[Ref.: #67770]	Host species	Trifolium repens

[Ref.: #120547]	Sample type/isolated from	Environment, Rhizosphere of clover, Trifolium repens
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence D16269 (>99% sequence identity) for Sporolactobacillus from Microbeatlas

Aquatic Samples	140
Soil Samples	181
Animal Samples	1421
Plant Samples	80
Total Samples	1822

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1038]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120547]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Sporolactobacillus laevolacticus gene for 16S rRNA, strain: IAM 12322	D16269	1484	ENA	33018 tax ID

[Ref.: #1038]	GC-content	36.7 mol%
[Ref.: #67770]	GC-content	43.5 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	44 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #1038]

Culture collection no.

DSM 2310, ATCC 23493, NCIB 10270, JCM 2514, IAM 12322, NCIMB 10270, CIP 104422

[Ref.: #83530]

SI-ID 46618

References

#1038

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2310

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#37933 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#83530

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID46618.1 )

#120547 Collection of Institut Pasteur ; Curators of the CIP; CIP 104422
* These data were automatically processed and therefore are not curated

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