Sporolactobacillus laevolacticus (DSM 2310, ATCC 23493, NCIB 10270)

Name: Sporolactobacillus laevolacticus (DSM 2310, ATCC 23493, NCIB 10270)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14387

Type strain

Strain Designation M-1

Culture col. no. DSM 2310 ATCC 23493 NCIB 10270 M-1 NCIMB 10270 3 more

NCBI tax ID(s) 33018

Special Collections DSMZ JCM CIP

History <- NCIB <- O. Nakayama, M-1 IAM 12322 <-- O. Nakayama M-1. CIP <- 1995, JCM <- IAM <- O. Nakamaya: strain M-1

Links

Sporolactobacillus laevolacticus M-1 is a facultative anaerobe, spore-forming, mesophilic prokaryote that produces lactate and was isolated from rhizosphere of Trifolium repens.

lactate production spore-forming Gram-positive motile rod-shaped facultative anaerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 46618

ATCC 23493,CIP 104422,DSM 2310,IAM 12322,JCM 2514,NCIMB 10270,NCIB 10270

@ref 20215

Last LPSN update 2025-12-16 09:50:20

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Sporolactobacillaceae

Genus Sporolactobacillus

Species Sporolactobacillus laevolacticus

Full scientific name Sporolactobacillus laevolacticus (Andersch et al. 1994 ex Nakayama and Yanoshi 1967) Hatayama et al. 2006

Synonyms (1)

Bacillus laevolacticus

BacDive ID	Other strains from **Sporolactobacillus laevolacticus** (20)	Type strain
14385	S. laevolacticus M-8, DSM 442, ATCC 23492, NCIB 10269, CCUG ... (type strain)
14386	S. laevolacticus M-14, DSM 444, ATCC 23496, NCIB 10274, JCM ...
14388	S. laevolacticus M-64, DSM 2315, NCIB 10277, JCM 3464, NCIMB ...
14389	S. laevolacticus M-7, DSM 2316, ATCC 23494, NCIB 10271, JCM ...
14390	S. laevolacticus M-40, DSM 2317, ATCC 23495, NCIB 10272, JCM ...
14391	S. laevolacticus M-4, DSM 2318, ATCC 23549, NCIB 10273
14392	S. laevolacticus M 71, DSM 6474, IAM 12329, JCM 3471
14393	S. laevolacticus M 104, DSM 6475, IAM 12331, JCM 3504
14394	S. laevolacticus M 105, DSM 6476, IAM 12326, JCM 3505
14395	S. laevolacticus M 81, DSM 6477, JCM 3481
24572	S. laevolacticus M 75, DSM 6478, IAM 12379, JCM 20530, JCM ...
24573	S. laevolacticus M89, DSM 6510, JCM 3489
24574	S. laevolacticus M121, DSM 6511, JCM 3521
24575	S. laevolacticus M 66, DSM 6547, IAM 12327, JCM 3466
24576	S. laevolacticus M 68, DSM 6548, IAM 12328, JCM 3468
24577	S. laevolacticus M 91, DSM 6549, IAM 12330, JCM 3491
24578	S. laevolacticus M 120, DSM 6763, JCM 3520
24579	S. laevolacticus M 95, DSM 6764, JCM 3495
24580	S. laevolacticus M 100, DSM 6771, JCM 3500
157158	S. laevolacticus CCUG 69853

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
120547	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
37933	MEDIUM 41- for Lactobacillus, Leuconostoc, Weissella, Pediococcus, Sporolactobacillus inulinus		Distilled water make up to (1000.000 ml);Man Rogosa Sharp broth (55.000 g)
1038	BACILLUS "RACEMILACTICUS" MEDIUM (DSMZ Medium 8)	Medium recipe at MediaDive	Name: BACILLUS "RACEMILACTICUS" MEDIUM (DSMZ Medium 8) Composition: Agar 15.0 g/l CaCO3 5.0 g/l Yeast extract 5.0 g/l Peptone 5.0 g/l Glucose 5.0 g/l Distilled water
1038	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
120547	CIP Medium 41	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
1038	positive	growth	30	mesophilic
37933	positive	growth	30	mesophilic
67770	positive	growth	30	mesophilic
120547	positive	growth	30-37	mesophilic
120547	negative	growth	10
120547	negative	growth	15
120547	negative	growth	45
120547	negative	growth	55

Physiology and metabolism

Oxygen tolerance

120547

Oxygen tolerancefacultative anaerobe

Spore formation

120547

Spore formationyes

Compound production

1038

CompoundD(-) lactic acid

Halophily

@ref	Salt	Growth	Tested relation	Concentration
120547	NaCl	positive	growth	0-2 %
120547	NaCl		growth	4 %
120547	NaCl		growth	6 %
120547	NaCl		growth	8 %
120547	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
120547	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	+	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	+	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	+	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	+	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
120547	17632 ChEBI	nitrate	-	reduction
120547	17632 ChEBI	nitrate	+	respiration
120547	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120547	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120547	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120547	amylase	+
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120547	caseinase	+	3.4.21.50
120547	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
120547	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
120547	gelatinase	-
120547	lecithinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
120547	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120547	ornithine decarboxylase	-	4.1.1.17
120547	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
120547	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120547	-	+	+	-	-	-	-	-	-	-	+	+	+	+	-	-	-	+	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
120547	not determinedn.d.	-	-	-	-	-	-	-	-	-	+	+	+	+	+	-	-	-	+	-	-	-	+	-	-	-	-	-	+	+/-	+	+	-	+	+	+	+/-	+/-	-	-	+	-	+	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
1038	rhizosphere of Trifolium repens	Trifolium repens	Kofu	Japan	JPN	Asia
67770	Rhizosphere of clover (Trifolium repens)	Trifolium repens
120547	Environment, Rhizosphere of clover, Trifolium repens

Taxonmaps

69479

Global distribution of 16S sequence D16269 (>99% sequence identity) for Sporolactobacillus from Microbeatlas

Aquatic Samples	140
Soil Samples	181
Animal Samples	1421
Plant Samples	80
Total Samples	1822

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1038	1	Risk group (German classification) According to TRBA 466
120547	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
67770	Sporolactobacillus laevolacticus gene for 16S rRNA, strain: IAM 12322	D16269	1484		33018

GC content

@ref	GC-content (mol%)	Method
1038	36.7
67770	43.5	thermal denaturation, midpoint method (Tm)
67770	44	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Title	Authors	Journal	DOI	Year
Antioxidant and Antibacterial Activities of Nano-probiotics Versus Free Probiotics Against Gastrointestinal Pathogenic Bacteria.	Al-Hazmi NE, Naguib DM.	Indian J Microbiol	10.1007/s12088-023-01140-2	2024

References

#1038	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2310
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#37933	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120547	Collection of Institut Pasteur ; Curators of the CIP; CIP 104422
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Sporolactobacillus laevolacticus (DSM 2310, ATCC 23493, NCIB 10270)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Metabolite utilization

Metabolite production

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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