Strain identifier

BacDive ID: 14387

Type strain: No

Species: Sporolactobacillus laevolacticus

Strain Designation: M-1

Strain history: CIP <- 1995, JCM <- IAM <- O. Nakamaya: strain M-1

NCBI tax ID(s): 33018 (species)

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General

@ref: 1038

BacDive-ID: 14387

DSM-Number: 2310

keywords: 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, lactate production

description: Sporolactobacillus laevolacticus M-1 is a facultative anaerobe, spore-forming, mesophilic bacterium that produces lactate and was isolated from rhizosphere of Trifolium repens.

NCBI tax id

  • NCBI tax id: 33018
  • Matching level: species

strain history

@refhistory
1038<- NCIB <- O. Nakayama, M-1
67770IAM 12322 <-- O. Nakayama M-1.
120547CIP <- 1995, JCM <- IAM <- O. Nakamaya: strain M-1

doi: 10.13145/bacdive14387.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Sporolactobacillaceae
  • genus: Sporolactobacillus
  • species: Sporolactobacillus laevolacticus
  • full scientific name: Sporolactobacillus laevolacticus (Andersch et al. 1994 ex Nakayama and Yanoshi 1967) Hatayama et al. 2006
  • synonyms

    • @ref: 20215
    • synonym: Bacillus laevolacticus

@ref: 1038

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Sporolactobacillaceae

genus: Sporolactobacillus

species: Sporolactobacillus laevolacticus

full scientific name: Sporolactobacillus laevolacticus (Andersch et al. 1994) Hatayama et al. 2006

strain designation: M-1

type strain: no

Morphology

cell morphology

  • @ref: 120547
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37933MEDIUM 41- for Lactobacillus, Leuconostoc, Weissella, Pediococcus, Sporolactobacillus inulinusyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp broth (55.000 g)
1038BACILLUS "RACEMILACTICUS" MEDIUM (DSMZ Medium 8)yesName: BACILLUS "RACEMILACTICUS" MEDIUM (DSMZ Medium 8) Composition: Agar 15.0 g/l CaCO3 5.0 g/l Yeast extract 5.0 g/l Peptone 5.0 g/l Glucose 5.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/8
1038R2A MEDIUM (DSMZ Medium 830)yesName: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled waterhttps://mediadive.dsmz.de/medium/830
120547CIP Medium 41yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=41

culture temp

@refgrowthtypetemperaturerange
1038positivegrowth30mesophilic
37933positivegrowth30mesophilic
67770positivegrowth30mesophilic
120547positivegrowth30-37mesophilic
120547nogrowth10psychrophilic
120547nogrowth15psychrophilic
120547nogrowth45thermophilic
120547nogrowth55thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120547
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 120547
  • spore formation: yes

compound production

  • @ref: 1038
  • compound: D(-) lactic acid

halophily

@refsaltgrowthtested relationconcentration
120547NaClpositivegrowth0-2 %
120547NaClnogrowth4 %
120547NaClnogrowth6 %
120547NaClnogrowth8 %
120547NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371raffinose+builds acid from16634
68371melezitose+builds acid from6731
68371inulin+builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose+builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120547citrate-carbon source16947
120547nitrate-reduction17632
120547nitrite-reduction16301
120547nitrate+respiration17632

metabolite production

  • @ref: 120547
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
120547oxidase-
120547alcohol dehydrogenase-1.1.1.1
120547gelatinase-
120547amylase+
120547DNase-
120547caseinase+3.4.21.50
120547catalase+1.11.1.6
120547lecithinase-
120547lysine decarboxylase-4.1.1.18
120547ornithine decarboxylase-4.1.1.17
120547urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120547-++-------++++---+--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120547---------+++++---+---+-----++/-++-++++/-+/---+-+-------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
1038rhizosphere of Trifolium repensTrifolium repensKofuJapanJPNAsia
67770Rhizosphere of clover (Trifolium repens)Trifolium repens
120547Environment, Rhizosphere of clover, Trifolium repens

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere

taxonmaps

  • @ref: 69479
  • File name: preview.99_7487.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_414;96_3495;97_4284;98_5485;99_7487&stattab=map
  • Last taxonomy: Sporolactobacillus
  • 16S sequence: D16269
  • Sequence Identity:
  • Total samples: 1822
  • soil counts: 181
  • aquatic counts: 140
  • animal counts: 1421
  • plant counts: 80

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
10381Risk group (German classification)
1205471Risk group (French classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Sporolactobacillus laevolacticus gene for 16S rRNA, strain: IAM 12322
  • accession: D16269
  • length: 1484
  • database: ena
  • NCBI tax ID: 33018

GC content

@refGC-contentmethod
103836.7
6777043.5thermal denaturation, midpoint method (Tm)
6777044thermal denaturation, midpoint method (Tm)

External links

@ref: 1038

culture collection no.: DSM 2310, ATCC 23493, NCIB 10270, JCM 2514, IAM 12322, NCIMB 10270, CIP 104422

straininfo link

  • @ref: 83530
  • straininfo: 46618

Reference

@idauthorscataloguedoi/urltitle
1038Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2310)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2310
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37933Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16329
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83530Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46618.1StrainInfo: A central database for resolving microbial strain identifiers
120547Curators of the CIPCollection of Institut Pasteur (CIP 104422)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104422