Strain identifier
BacDive ID: 14387
Type strain:
Species: Sporolactobacillus laevolacticus
Strain Designation: M-1
Strain history: CIP <- 1995, JCM <- IAM <- O. Nakamaya: strain M-1
NCBI tax ID(s): 33018 (species)
General
@ref: 1038
BacDive-ID: 14387
DSM-Number: 2310
keywords: 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, lactate production
description: Sporolactobacillus laevolacticus M-1 is a facultative anaerobe, spore-forming, mesophilic bacterium that produces lactate and was isolated from rhizosphere of Trifolium repens.
NCBI tax id
- NCBI tax id: 33018
- Matching level: species
strain history
@ref | history |
---|---|
1038 | <- NCIB <- O. Nakayama, M-1 |
67770 | IAM 12322 <-- O. Nakayama M-1. |
120547 | CIP <- 1995, JCM <- IAM <- O. Nakamaya: strain M-1 |
doi: 10.13145/bacdive14387.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Sporolactobacillaceae
- genus: Sporolactobacillus
- species: Sporolactobacillus laevolacticus
- full scientific name: Sporolactobacillus laevolacticus (Andersch et al. 1994 ex Nakayama and Yanoshi 1967) Hatayama et al. 2006
synonyms
- @ref: 20215
- synonym: Bacillus laevolacticus
@ref: 1038
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Sporolactobacillaceae
genus: Sporolactobacillus
species: Sporolactobacillus laevolacticus
full scientific name: Sporolactobacillus laevolacticus (Andersch et al. 1994) Hatayama et al. 2006
strain designation: M-1
type strain: no
Morphology
cell morphology
- @ref: 120547
- gram stain: positive
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
37933 | MEDIUM 41- for Lactobacillus, Leuconostoc, Weissella, Pediococcus, Sporolactobacillus inulinus | yes | Distilled water make up to (1000.000 ml);Man Rogosa Sharp broth (55.000 g) | |
1038 | BACILLUS "RACEMILACTICUS" MEDIUM (DSMZ Medium 8) | yes | Name: BACILLUS "RACEMILACTICUS" MEDIUM (DSMZ Medium 8) Composition: Agar 15.0 g/l CaCO3 5.0 g/l Yeast extract 5.0 g/l Peptone 5.0 g/l Glucose 5.0 g/l Distilled water | https://mediadive.dsmz.de/medium/8 |
1038 | R2A MEDIUM (DSMZ Medium 830) | yes | Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water | https://mediadive.dsmz.de/medium/830 |
120547 | CIP Medium 41 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=41 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
1038 | positive | growth | 30 | mesophilic |
37933 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
120547 | positive | growth | 30-37 | mesophilic |
120547 | no | growth | 10 | psychrophilic |
120547 | no | growth | 15 | psychrophilic |
120547 | no | growth | 45 | thermophilic |
120547 | no | growth | 55 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 120547
- oxygen tolerance: facultative anaerobe
spore formation
- @ref: 120547
- spore formation: yes
compound production
- @ref: 1038
- compound: D(-) lactic acid
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
120547 | NaCl | positive | growth | 0-2 % |
120547 | NaCl | no | growth | 4 % |
120547 | NaCl | no | growth | 6 % |
120547 | NaCl | no | growth | 8 % |
120547 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | + | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | + | builds acid from | 32528 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | raffinose | + | builds acid from | 16634 |
68371 | melezitose | + | builds acid from | 6731 |
68371 | inulin | + | builds acid from | 15443 |
68371 | trehalose | - | builds acid from | 27082 |
68371 | sucrose | + | builds acid from | 17992 |
68371 | melibiose | + | builds acid from | 28053 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | - | builds acid from | 17057 |
68371 | salicin | - | builds acid from | 17814 |
68371 | esculin | - | builds acid from | 4853 |
68371 | arbutin | - | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | + | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | + | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | + | builds acid from | 17266 |
68371 | D-mannose | + | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | + | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | D-ribose | - | builds acid from | 16988 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | - | builds acid from | 17754 |
120547 | citrate | - | carbon source | 16947 |
120547 | nitrate | - | reduction | 17632 |
120547 | nitrite | - | reduction | 16301 |
120547 | nitrate | + | respiration | 17632 |
metabolite production
- @ref: 120547
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
120547 | oxidase | - | |
120547 | alcohol dehydrogenase | - | 1.1.1.1 |
120547 | gelatinase | - | |
120547 | amylase | + | |
120547 | DNase | - | |
120547 | caseinase | + | 3.4.21.50 |
120547 | catalase | + | 1.11.1.6 |
120547 | lecithinase | - | |
120547 | lysine decarboxylase | - | 4.1.1.18 |
120547 | ornithine decarboxylase | - | 4.1.1.17 |
120547 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | - | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | + | 3.2.1.22 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120547 | - | + | + | - | - | - | - | - | - | - | + | + | + | + | - | - | - | + | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120547 | - | - | - | - | - | - | - | - | - | + | + | + | + | + | - | - | - | + | - | - | - | + | - | - | - | - | - | + | +/- | + | + | - | + | + | + | +/- | +/- | - | - | + | - | + | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | geographic location | country | origin.country | continent |
---|---|---|---|---|---|---|
1038 | rhizosphere of Trifolium repens | Trifolium repens | Kofu | Japan | JPN | Asia |
67770 | Rhizosphere of clover (Trifolium repens) | Trifolium repens | ||||
120547 | Environment, Rhizosphere of clover, Trifolium repens |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
#Host Body-Site | #Plant | #Rhizosphere |
taxonmaps
- @ref: 69479
- File name: preview.99_7487.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_414;96_3495;97_4284;98_5485;99_7487&stattab=map
- Last taxonomy: Sporolactobacillus
- 16S sequence: D16269
- Sequence Identity:
- Total samples: 1822
- soil counts: 181
- aquatic counts: 140
- animal counts: 1421
- plant counts: 80
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
1038 | 1 | Risk group (German classification) |
120547 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 67770
- description: Sporolactobacillus laevolacticus gene for 16S rRNA, strain: IAM 12322
- accession: D16269
- length: 1484
- database: ena
- NCBI tax ID: 33018
GC content
@ref | GC-content | method |
---|---|---|
1038 | 36.7 | |
67770 | 43.5 | thermal denaturation, midpoint method (Tm) |
67770 | 44 | thermal denaturation, midpoint method (Tm) |
External links
@ref: 1038
culture collection no.: DSM 2310, ATCC 23493, NCIB 10270, JCM 2514, IAM 12322, NCIMB 10270, CIP 104422
straininfo link
- @ref: 83530
- straininfo: 46618
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
1038 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2310) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2310 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
37933 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/16329 | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
68371 | Automatically annotated from API 50CH acid | |||
68382 | Automatically annotated from API zym | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
83530 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID46618.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
120547 | Curators of the CIP | Collection of Institut Pasteur (CIP 104422) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104422 |