Sporolactobacillus laevolacticus (DSM 442, ATCC 23492, NCIB 10269)

Name: Sporolactobacillus laevolacticus (DSM 442, ATCC 23492, NCIB 10269)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14385

Type strain

Strain Designation M-8

Culture col. no. DSM 442 ATCC 23492 NCIB 10269 CCUG 33623 LMG 16675 10 more

NCBI tax ID(s) 1395513 33018

Special Collections DSMZ CCUG JCM KCTC CIP

History <- O. Nakayama, M-8 IAM 12321 <-- O. Nakayama M-8. CIP <- 1995, JCM <- IAM <- O. Nakamaya: strain M-8

Links

Sporolactobacillus laevolacticus M-8 is an anaerobe, spore-forming, mesophilic prokaryote that produces lactate and was isolated from rhizosphere of ditch crowfoot.

lactate production spore-forming Gram-positive motile rod-shaped anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 92157

LMG 6329,ATCC 23492,CCRC 11598,CCUG 33623,CIP 104421,DSM 442,IAM 12321,JCM 2513,KCTC 3117,LMG 16675,NCIMB 10269,NCIB 10269,LMD 86.41,CECT 692,KCTC 3311,BCRC 11598,NCCB 86041,NCIM 2464,NBRC 102473

@ref 20215

Last LPSN update 2026-02-09 11:21:24

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Sporolactobacillaceae

Genus Sporolactobacillus

Species Sporolactobacillus laevolacticus

Full scientific name Sporolactobacillus laevolacticus (Andersch et al. 1994 ex Nakayama and Yanoshi 1967) Hatayama et al. 2006

Synonyms (1)

Bacillus laevolacticus

BacDive ID	Other strains from **Sporolactobacillus laevolacticus** (20)	Type strain
14386	S. laevolacticus M-14, DSM 444, ATCC 23496, NCIB 10274, JCM ...
14387	S. laevolacticus M-1, DSM 2310, ATCC 23493, NCIB 10270, JCM ...
14388	S. laevolacticus M-64, DSM 2315, NCIB 10277, JCM 3464, NCIMB ...
14389	S. laevolacticus M-7, DSM 2316, ATCC 23494, NCIB 10271, JCM ...
14390	S. laevolacticus M-40, DSM 2317, ATCC 23495, NCIB 10272, JCM ...
14391	S. laevolacticus M-4, DSM 2318, ATCC 23549, NCIB 10273
14392	S. laevolacticus M 71, DSM 6474, IAM 12329, JCM 3471
14393	S. laevolacticus M 104, DSM 6475, IAM 12331, JCM 3504
14394	S. laevolacticus M 105, DSM 6476, IAM 12326, JCM 3505
14395	S. laevolacticus M 81, DSM 6477, JCM 3481
24572	S. laevolacticus M 75, DSM 6478, IAM 12379, JCM 20530, JCM ...
24573	S. laevolacticus M89, DSM 6510, JCM 3489
24574	S. laevolacticus M121, DSM 6511, JCM 3521
24575	S. laevolacticus M 66, DSM 6547, IAM 12327, JCM 3466
24576	S. laevolacticus M 68, DSM 6548, IAM 12328, JCM 3468
24577	S. laevolacticus M 91, DSM 6549, IAM 12330, JCM 3491
24578	S. laevolacticus M 120, DSM 6763, JCM 3520
24579	S. laevolacticus M 95, DSM 6764, JCM 3495
24580	S. laevolacticus M 100, DSM 6771, JCM 3500
157158	S. laevolacticus CCUG 69853

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
120546	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
208	CASO AGAR (MERCK 105458) (DSMZ Medium 220)	Medium recipe at MediaDive	Name: CASO AGAR (MERCK 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
41166	MEDIUM 41- for Lactobacillus, Leuconostoc, Weissella, Pediococcus, Sporolactobacillus inulinus		Distilled water make up to (1000.000 ml);Man Rogosa Sharp broth (55.000 g)
208	BACILLUS "RACEMILACTICUS" MEDIUM (DSMZ Medium 8)	Medium recipe at MediaDive	Name: BACILLUS "RACEMILACTICUS" MEDIUM (DSMZ Medium 8) Composition: Agar 15.0 g/l CaCO3 5.0 g/l Yeast extract 5.0 g/l Peptone 5.0 g/l Glucose 5.0 g/l Distilled water
120546	CIP Medium 41	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
208	positive	growth	30	mesophilic
41166	positive	growth	30	mesophilic
51643	positive	growth	37	mesophilic
67770	positive	growth	30	mesophilic
120546	positive	growth	18-45
120546	negative	growth	15

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
51643	anaerobe
120546	facultative anaerobe
125439	obligate aerobe	97

Spore formation

120546

Spore formationyes

Compound production

208

CompoundD(-) lactic acid

Halophily

@ref	Salt	Growth	Tested relation	Concentration
120546	NaCl	positive	growth	0-2 %
120546	NaCl		growth	4 %
120546	NaCl		growth	6 %
120546	NaCl		growth	8 %
120546	NaCl		growth	10 %

Observation

67770

Observationquinones: MK-7

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68381	40585 ChEBI	alpha-cyclodextrin	-	builds acid from	from API rID32STR
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68368	27613 ChEBI	amygdalin	-	fermentation	from API 20E
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68381	29016 ChEBI	arginine	-	hydrolysis	from API rID32STR
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68381	18333 ChEBI	D-arabitol	-	builds acid from	from API rID32STR
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68368	17634 ChEBI	D-glucose	-	fermentation	from API 20E
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68381	16899 ChEBI	D-mannitol	-	builds acid from	from API rID32STR
68368	16899 ChEBI	D-mannitol	-	fermentation	from API 20E
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68381	16988 ChEBI	D-ribose	-	builds acid from	from API rID32STR
68381	16443 ChEBI	D-tagatose	-	builds acid from	from API rID32STR
68371	16443 ChEBI	D-tagatose	+	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68381	28087 ChEBI	glycogen	-	builds acid from	from API rID32STR
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68381	606565 ChEBI	hippurate	-	hydrolysis	from API rID32STR
68381	30849 ChEBI	L-arabinose	-	builds acid from	from API rID32STR
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68368	30849 ChEBI	L-arabinose	-	fermentation	from API 20E
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68368	62345 ChEBI	L-rhamnose	-	fermentation	from API 20E
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68381	17716 ChEBI	lactose	-	builds acid from	from API rID32STR
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68381	17306 ChEBI	maltose	-	builds acid from	from API rID32STR
68381	6731 ChEBI	melezitose	-	builds acid from	from API rID32STR
68371	6731 ChEBI	melezitose	+	builds acid from	from API 50CH acid
68381	28053 ChEBI	melibiose	-	builds acid from	from API rID32STR
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68368	28053 ChEBI	melibiose	-	fermentation	from API 20E
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68381	320055 ChEBI	methyl beta-D-glucopyranoside	-	builds acid from	from API rID32STR
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68368	17268 ChEBI	myo-inositol	-	fermentation	from API 20E
120546	17632 ChEBI	nitrate	-	reduction
120546	17632 ChEBI	nitrate	+	respiration
120546	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68381	27941 ChEBI	pullulan	-	builds acid from	from API rID32STR
68381	16634 ChEBI	raffinose	-	builds acid from	from API rID32STR
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68381	30911 ChEBI	sorbitol	-	builds acid from	from API rID32STR
68368	30911 ChEBI	sorbitol	-	fermentation	from API 20E
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68381	17992 ChEBI	sucrose	-	builds acid from	from API rID32STR
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68368	17992 ChEBI	sucrose	-	fermentation	from API 20E
68381	27082 ChEBI	trehalose	-	builds acid from	from API rID32STR
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68381	16199 ChEBI	urea	-	hydrolysis	from API rID32STR
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite
68381	15688 ChEBI	acetoin	from API rID32STR
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
120546	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68381	15688 ChEBI	acetoin	+		from API rID32STR
68368	15688 ChEBI	acetoin	-		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68381	Alanyl-Phenylalanyl-Proline arylamidase	-		from API rID32STR
120546	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68381	alkaline phosphatase	-	3.1.3.1	from API rID32STR
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68381	alpha-galactosidase	+	3.2.1.22	from API rID32STR
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120546	amylase	-
68381	arginine dihydrolase	-	3.5.3.6	from API rID32STR
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68381	beta-galactosidase	-	3.2.1.23	from API rID32STR
68382	beta-galactosidase	-	3.2.1.23	from API zym
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68381	beta-glucosidase	-	3.2.1.21	from API rID32STR
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68381	beta-glucuronidase	-	3.2.1.31	from API rID32STR
68381	beta-mannosidase	-	3.2.1.25	from API rID32STR
120546	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68368	gelatinase	-		from API 20E
68381	glycyl tryptophan arylamidase	-		from API rID32STR
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
120546	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68381	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32STR
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120546	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68381	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32STR
68382	trypsin	-	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68381	urease	-	3.5.1.5	from API rID32STR
120546	urease	-	3.5.1.5
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	teichoic acid biosynthesis	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	gluconeogenesis	100	8 of 8
66794	aspartate and asparagine metabolism	100	9 of 9
66794	ethanol fermentation	100	2 of 2
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	pentose phosphate pathway	100	11 of 11
66794	cyanate degradation	100	3 of 3
66794	vitamin K metabolism	100	5 of 5
66794	lipoate biosynthesis	100	5 of 5
66794	aerobactin biosynthesis	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	acetate fermentation	100	4 of 4
66794	folate polyglutamylation	100	1 of 1
66794	ribulose monophosphate pathway	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	acetoin degradation	100	3 of 3
66794	methylglyoxal degradation	100	5 of 5
66794	palmitate biosynthesis	95.45	21 of 22
66794	heme metabolism	92.86	13 of 14
66794	pyrimidine metabolism	91.11	41 of 45
66794	threonine metabolism	90	9 of 10
66794	starch degradation	90	9 of 10
66794	valine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	degradation of sugar alcohols	87.5	14 of 16
66794	methionine metabolism	80.77	21 of 26
66794	flavin biosynthesis	80	12 of 15
66794	peptidoglycan biosynthesis	80	12 of 15
66794	glycine betaine biosynthesis	80	4 of 5
66794	cellulose degradation	80	4 of 5
66794	Entner Doudoroff pathway	80	8 of 10
66794	glycogen metabolism	80	4 of 5
66794	photosynthesis	78.57	11 of 14
66794	allantoin degradation	77.78	7 of 9
66794	serine metabolism	77.78	7 of 9
66794	purine metabolism	77.66	73 of 94
66794	phenylalanine metabolism	76.92	10 of 13
66794	alanine metabolism	75.86	22 of 29
66794	isoleucine metabolism	75	6 of 8
66794	glycogen biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	NAD metabolism	72.22	13 of 18
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	glutamate and glutamine metabolism	71.43	20 of 28
66794	glycolysis	70.59	12 of 17
66794	vitamin B1 metabolism	69.23	9 of 13
66794	leucine metabolism	69.23	9 of 13
66794	non-pathway related	68.42	26 of 38
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	methane metabolism	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	d-mannose degradation	66.67	6 of 9
66794	enterobactin biosynthesis	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	histidine metabolism	65.52	19 of 29
66794	glutathione metabolism	64.29	9 of 14
66794	lysine metabolism	64.29	27 of 42
66794	ketogluconate metabolism	62.5	5 of 8
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	isoprenoid biosynthesis	61.54	16 of 26
66794	3-chlorocatechol degradation	60	3 of 5
66794	oxidative phosphorylation	59.34	54 of 91
66794	arginine metabolism	58.33	14 of 24
66794	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
66794	citric acid cycle	57.14	8 of 14
66794	tetrahydrofolate metabolism	57.14	8 of 14
66794	propanol degradation	57.14	4 of 7
66794	polyamine pathway	56.52	13 of 23
66794	cysteine metabolism	55.56	10 of 18
66794	tryptophan metabolism	55.26	21 of 38
66794	proline metabolism	54.55	6 of 11
66794	urea cycle	53.85	7 of 13
66794	suberin monomers biosynthesis	50	1 of 2
66794	biotin biosynthesis	50	2 of 4
66794	CMP-KDO biosynthesis	50	2 of 4
66794	arachidonic acid metabolism	50	9 of 18
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	kanosamine biosynthesis II	50	1 of 2
66794	adipate degradation	50	1 of 2
66794	sulfopterin metabolism	50	2 of 4
66794	lactate fermentation	50	2 of 4
66794	glycine metabolism	50	5 of 10
66794	phenylmercury acetate degradation	50	1 of 2
66794	butanoate fermentation	50	2 of 4
66794	tyrosine metabolism	50	7 of 14
66794	phosphatidylethanolamine bioynthesis	46.15	6 of 13
66794	metabolism of disaccharids	45.45	5 of 11
66794	CO2 fixation in Crenarchaeota	44.44	4 of 9
66794	degradation of hexoses	44.44	8 of 18
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	gallate degradation	40	2 of 5
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	hydrogen production	40	2 of 5
66794	degradation of pentoses	39.29	11 of 28
66794	lipid metabolism	38.71	12 of 31
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	vitamin B6 metabolism	36.36	4 of 11
66794	phenol degradation	35	7 of 20
66794	pantothenate biosynthesis	33.33	2 of 6
66794	sulfoquinovose degradation	33.33	1 of 3
66794	nitrate assimilation	33.33	3 of 9
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	lipid A biosynthesis	33.33	3 of 9
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	molybdenum cofactor biosynthesis	33.33	3 of 9
66794	coenzyme M biosynthesis	30	3 of 10
66794	4-hydroxyphenylacetate degradation	30	3 of 10
66794	propionate fermentation	30	3 of 10
66794	myo-inositol biosynthesis	30	3 of 10
66794	degradation of sugar acids	28	7 of 25
66794	ascorbate metabolism	27.27	6 of 22
66794	d-xylose degradation	27.27	3 of 11
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	androgen and estrogen metabolism	25	4 of 16
66794	sulfate reduction	23.08	3 of 13
66794	4-hydroxymandelate degradation	22.22	2 of 9

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
51643	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120546	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
51643	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	+	+	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-
51643	-	-	-	-	-	-	-	-	-	-	+	+	+	+	+	-	-	-	+	-	-	-	+	-	-	-	-	-	+	+	+	+	+	+	+	+	-	-	-	-	+	-	+	-	-	-	-	-	-	-
120546	not determinedn.d.	-	-	-	-	-	-	-	-	-	+	+	+	+	+	-	-	-	+	+	-	-	+	-	-	-	-	-	+	+/-	+	+	+	+	+	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-

API rID32STR

@ref	ADH (Arg)	beta GLU	beta GAR	beta GUR	alpha GAL	PAL	RIB	MAN	SOR	LAC	TRE	RAF	SAC	LARA	DARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	HIP	GLYG	PUL	MAL	MEL	MLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	TAG	beta MAN	URE
51643	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
120546	not determinedn.d.	+	+	+	+	+	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Host species
208	rhizosphere of ditch crowfoot	Kofu	Japan	JPN	Asia
51643	Rhizosphere of ditch crowfoot
67770	Rhizosphere of ditch crowfoot (Ranunculus sceleratus)					Ranunculus sceleratus
120546	Environment, Ditch crowfoot, rhizosphere: Ranunculus

Taxonmaps

69479

Global distribution of 16S sequence AB374516 (>99% sequence identity) for Sporolactobacillus from Microbeatlas

Aquatic Samples	140
Soil Samples	181
Animal Samples	1421
Plant Samples	80
Total Samples	1822

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
208	1	Risk group (German classification) According to TRBA 466
120546	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	DSM442V1 assembly for Sporolactobacillus laevolacticus DSM 442	contig	GCA_000497245	1395513.3	2554235469	1395513	59.6

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Sporolactobacillus laevolacticus gene for 16S rRNA, partial sequence, strain: NRIC 0361 (= ATCC 23492, = JCM 2513)	AB362642	1545	33018
20218	Sporolactobacillus laevolacticus gene for 16S ribosomal RNA, partial sequence	AB374516	1503	33018
20218	Sporolactobacillus laevolacticus gene for 16S rRNA, strain: IAM 12321	D16270	1484	33018
20218	Sporolactobacillus laevolacticus gene for 16S rRNA, partial sequence	AB371636	1476	33018

GC content

@ref	GC-content (mol%)	Method
208	41.5
67770	44.7	thermal denaturation, midpoint method (Tm)
67770	43	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Sporolactobacillus laevolacticus DSM 442
NCBI: GCA_000497245

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	83.20	no
125439	motility	BacteriaNetⓘ	yes	76.40	no
125439	gram_stain	BacteriaNetⓘ	variable	80.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Sporolactobacillus laevolacticus DSM 442
PATRIC: 1395513.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	77.05	no
125438	anaerobic	anaerobicⓘ	no	80.31	no
125438	spore-forming	spore-formingⓘ	yes	80.24	no
125438	aerobic	aerobicⓘ	no	76.66	yes
125438	thermophilic	thermophileⓘ	no	89.31	no
125438	flagellated	motile2+ⓘ	yes	78.74	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Who is who in necromass formation and stabilization in soil? The role of fungi and bacteria as complementary players of biogeochemical functioning.	Lepori S, Rohner N, Li X, Feng X, Wagai R, Loaiza V, Sebag D, Verrecchia E, Nelson DB, Kahmen A, Chenu C, Niklaus PA, Laine AL, Domeignoz-Horta LA.	ISME Commun	10.1093/ismeco/ycaf186	2025
Genetics	Genome Sequence of Sporolactobacillus laevolacticus DSM442, an Efficient Polymer-Grade D-Lactate Producer from Agricultural Waste Cottonseed as a Nitrogen Source.	Wang H, Wang L, Ju J, Yu B, Ma Y	Genome Announc	10.1128/genomeA.01100-13	2013
Biotechnology	Efficient production of polymer-grade D-lactate by Sporolactobacillus laevolacticus DSM442 with agricultural waste cottonseed as the sole nitrogen source.	Li Y, Wang L, Ju J, Yu B, Ma Y	Bioresour Technol	10.1016/j.biortech.2013.04.124	2013
	Sporolactobacillus mangiferae sp. nov., a spore-forming lactic acid bacterium isolated from tree bark in Thailand.	Phuengjayaem S, Poothong S, Sitdhipol J, Chaiyawan N, Thitiprasert S, Thongchul N, Tanasupawat S.	Int J Syst Evol Microbiol	10.1099/ijsem.0.005993	2023

References

#208	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 442
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#41166	; Curators of the CIP;
#51643	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 33623
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68371	Automatically annotated from API 50CH acid .
#68381	Automatically annotated from API rID32STR .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120546	Collection of Institut Pasteur ; Curators of the CIP; CIP 104421
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Content

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Sporolactobacillus laevolacticus (DSM 442, ATCC 23492, NCIB 10269)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

API 20E

API zym

API 50CHac

API rID32STR

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000497245

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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