Lactobacillus helveticus (CIP 104344, NCDO 99, NCIMB 700099)

Name: Lactobacillus helveticus (CIP 104344, NCDO 99, NCIMB 700099)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 139090

Type strain

Strain Designation B2

Culture col. no. CIP 104344 NCDO 99 NCIMB 700099 LMG 11446 JCM 1004 1 more

NCBI tax ID(s) 1587

Special Collections JCM CIP

History JCM <-- T. Mitsuoka S1-15 <-- M. E. Sharpe J-8 <-- NCDO 99. CIP <- 1995, F. Gasser, Inst. Pasteur, Paris, France: strain B2 <- NCDO <- 1954, M.E. Sharpe, NIRD <- J.M. Sherman, Cornell Univ., "Lactobacillus jugurt", Lactobacillus bulgaricus

Links

Lactobacillus helveticus B2 is a facultative anaerobe, Gram-positive, rod-shaped bacterium of the family Lactobacillaceae.

Gram-positive rod-shaped facultative anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 3305

LMG 11446,JCM 1004,NCFB 99,CIP 104344,NCDO 99,NCIMB 700099

@ref 20215

Last LPSN update 2026-05-29 08:39:24

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Lactobacillus

Species Lactobacillus helveticus

Full scientific name Lactobacillus helveticus (Orla-Jensen 1919) Bergey et al. 1925 (Approved Lists 1980)

Synonyms (2)

Lactobacillus suntoryeus
"Thermobacterium helveticum"

BacDive ID	Other strains from **Lactobacillus helveticus** (23)	Type strain
6471	L. helveticus 12, Lh12, DSM 20075, ATCC 15009, CCUG 30139, ... (type strain)
6525	L. helveticus CBP1, DSM 3748
134852	L. helveticus CIP 76.19, ATCC 521, JCM 1003, NCDO 87, NCIMB ...
136298	L. helveticus H2, CIP 104345, ATCC 10386, NCDO 261, NCIMB ...
136299	L. helveticus J2, CIP 104346
136381	L. helveticus 5, CIP 53.165, ATCC 7995, JCM 11039
137106	L. helveticus H-80, CIP 57.15, ATCC 12046, JCM 1554, NCDO ...
137173	L. helveticus J5, CIP 104343, ATCC 10812, IFO 3809, NCDO ...
139247	L. helveticus CIP 104436, ATCC 7993
153568	L. helveticus CCUG 50205, NCIMB 14005, JCM 30912, BCRC ...
154986	L. helveticus CCUG 55959
160849	L. helveticus JCM 1005, ATCC 8001, CECT 404, NCFB 103, ...
160853	L. helveticus JCM 1006, LMG 18174
160862	L. helveticus JCM 1007, ATCC 10797, LMG 11474
160865	L. helveticus JCM 1008, ATCC 8018, CECT 414, LMG 11449, ...
161004	L. helveticus JCM 1062, BCRC 14035, LMG 18182
161122	L. helveticus JCM 1103, LMG 18183
162696	L. helveticus JCM 20023, ATU L-21, IAM 1042
162913	L. helveticus JCM 20397, IAM 12090
172918	L. helveticus 61 BZ, CRBIP24.47
172919	L. helveticus 60 A, CRBIP24.54
172920	L. helveticus 60 B, CRBIP24.55
172921	L. helveticus 64 AB, CRBIP24.65

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
41132	positive	rod-shaped
125438			92
125438	positive		93.551

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition	Medium link
41132	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
41132	CIP Medium 40			Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
41132	positive	growth	37
41132	positive	growth	22-45
41132	negative	growth	10
41132	negative	growth	15
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
41132	facultative anaerobe
125439	facultative anaerobe	94.641

Spore formation

@ref	Spore formation	Confidence
125438		91.352

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
41132	17632 ChEBI	nitrate	-	reduction
41132	17632 ChEBI	nitrate	+	respiration
41132	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
41132	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
41132	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
41132	catalase	-	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
41132	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
41132	ornithine decarboxylase	-	4.1.1.17
41132	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
41132	-	-	-	-	-	+	-	+	-	-	+	+	-	+	-	+	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
41132	not determinedn.d.	-	-	-	-	-	-	-	-	-	+	+	-	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+/-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
41132	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM1840845v1 assembly for Lactobacillus helveticus JCM 1004	complete	GCA_018408455	1587.708	1587	96.64

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
124043	Lactobacillus helveticus gene for 16S rRNA, partial sequence, strain: JCM 1004.	AB289148	647		1587

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1840845v1 assembly for Lactobacillus helveticus JCM 1004
BV-BRC: 1587.708

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	94.64	no
125439	motility	BacteriaNetⓘ	yes	55.87	no
125439	gram_stain	BacteriaNetⓘ	positive	50.69	no
125439	spore_formation	BacteriaNetⓘ	yes	40.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus helveticus JCM 1004
NCBI: GCA_018408455.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	93.55	no
125438	anaerobic	anaerobicⓘ	no	70.76	no
125438	aerobic	aerobicⓘ	no	97.03	yes
125438	spore-forming	spore-formingⓘ	no	91.35	no
125438	thermophilic	thermophileⓘ	no	96.00	no
125438	flagellated	motile2+ⓘ	no	92.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	A Lactobacillus helveticus-Specific DNA Probe Detects Restriction Fragment Length Polymorphisms in This Species.	de Los Reyes-Gavilan CG, Limsowtin GK, Tailliez P, Sechaud L, Accolas JP.	Appl Environ Microbiol	10.1128/aem.58.10.3429-3432.1992	1992
	Zymogram and Preliminary Characterization of Lactobacillus helveticus Autolysins.	Valence F, Lortal S.	Appl Environ Microbiol	10.1128/aem.61.9.3391-3399.1995	1995
	Comparative Study of 35 Bacteriophages of Lactobacillus helveticus: Morphology and Host Range.	Sechaud L, Rousseau M, Fayard B, Callegari ML, Quenee P, Accolas JP.	Appl Environ Microbiol	10.1128/aem.58.3.1011-1018.1992	1992
	Genetic diversity in the lactose operons of Lactobacillus helveticus strains and its relationship to the role of these strains as commercial starter cultures.	Callanan MJ, Beresford TP, Ross RP.	Appl Environ Microbiol	10.1128/aem.71.3.1655-1658.2005	2005
	Identification and cultivation of anaerobic bacterial scavengers of dead cells.	Hirakata Y, Mei R, Morinaga K, Katayama T, Tamaki H, Meng XY, Watari T, Yamaguchi T, Hatamoto M, Nobu MK.	ISME J	10.1038/s41396-023-01538-2	2023
Metabolism	Characterization of the recombinant exopeptidases PepX and PepN from Lactobacillus helveticus ATCC 12046 important for food protein hydrolysis.	Stressler T, Eisele T, Schlayer M, Lutz-Wahl S, Fischer L.	PLoS One	10.1371/journal.pone.0070055	2013
	Purification and characterization of an aminopeptidase from Lactobacillus helveticus JCM 1004	Pan D, Tanokura M.	Food Chem	10.1016/j.foodchem.2004.01.082	2004
Genetics	Genome sequence and analysis of Lactobacillus helveticus.	Cremonesi P, Chessa S, Castiglioni B.	Front Microbiol	10.3389/fmicb.2012.00435	2012
Genetics	Complete Genome Sequence of Lactobacillus helveticus JCM 1004, an Aminopeptidase-Producing Lactic Acid Bacterium.	Morinaga K, Kusada H, Watanabe M, Tamaki H	Microbiol Resour Announc	10.1128/MRA.00641-21	2021

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#41132	Collection of Institut Pasteur ; Curators of the CIP; CIP 104344
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lactobacillus helveticus (CIP 104344, NCDO 99, NCIMB 700099)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_018408455

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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