Strain identifier

BacDive ID: 139090

Type strain: No

Species: Lactobacillus helveticus

Strain Designation: B2

Strain history: CIP <- 1995, F. Gasser, Inst. Pasteur, Paris, France: strain B2 <- NCDO <- 1954, M.E. Sharpe, NIRD <- J.M. Sherman, Cornell Univ., "Lactobacillus jugurt", Lactobacillus bulgaricus

NCBI tax ID(s): 1587 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.3 (current version):
version 9.2:
version 9.1:
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 9.3 (current version)

General

@ref: 41132

BacDive-ID: 139090

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive

description: Lactobacillus helveticus B2 is a facultative anaerobe, mesophilic, Gram-positive bacterium of the family Lactobacillaceae.

NCBI tax id

  • NCBI tax id: 1587
  • Matching level: species

strain history

@refhistory
67770T. Mitsuoka S1-15 <-- M. E. Sharpe J-8 <-- NCDO 99.
41132CIP <- 1995, F. Gasser, Inst. Pasteur, Paris, France: strain B2 <- NCDO <- 1954, M.E. Sharpe, NIRD <- J.M. Sherman, Cornell Univ., "Lactobacillus jugurt", Lactobacillus bulgaricus

doi: 10.13145/bacdive139090.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus helveticus
  • full scientific name: Lactobacillus helveticus (Orla-Jensen 1919) Bergey et al. 1925 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Lactobacillus suntoryeus
    20215Thermobacterium helveticum

@ref: 41132

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus helveticus

strain designation: B2

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
125438no92
12543893.551positive
41132nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41132MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
41132CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
41132positivegrowth37
67770positivegrowth37
41132positivegrowth22-45
41132nogrowth10
41132nogrowth15

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
41132facultative anaerobe
125439microaerophile94.5

spore formation

  • @ref: 125438
  • spore formation: no
  • confidence: 91.352

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
41132nitrate-reduction17632
41132nitrite-reduction16301
41132nitrate+respiration17632

metabolite tests

  • @ref: 41132
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
41132oxidase-
41132alcohol dehydrogenase-1.1.1.1
41132catalase-1.11.1.6
41132lysine decarboxylase-4.1.1.18
41132ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
41132-----+-+--++-+-+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
41132---------++-+--------+-----+/-+--+-----------------

Safety information

risk assessment

  • @ref: 41132
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 124043
  • description: Lactobacillus helveticus gene for 16S rRNA, partial sequence, strain: JCM 1004.
  • accession: AB289148
  • length: 647
  • database: nuccore
  • NCBI tax ID: 1587

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus helveticus JCM1004GCA_018408455completencbi1587
66792Lactobacillus helveticus strain JCM 1004 strain JCM10041587.708completepatric1587

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes93.551no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no70.759no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no97.032yes
125438spore-formingspore-formingAbility to form endo- or exosporesno91.352no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno96no
125438motile2+flagellatedAbility to perform flagellated movementno92no
125439BacteriaNetspore_formationAbility to form endo- or exosporesyes54.7
125439BacteriaNetmotilityAbility to perform movementyes65
125439BacteriaNetgram_stainReaction to gram-stainingpositive69.4
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthmicroaerophile94.5

External links

@ref: 41132

culture collection no.: CIP 104344, NCDO 99, JCM 1004, LMG 11446, NCIMB 700099

straininfo link

  • @ref: 95671
  • straininfo: 3305

literature

  • topic: Genetics
  • Pubmed-ID: 34410159
  • title: Complete Genome Sequence of Lactobacillus helveticus JCM 1004, an Aminopeptidase-Producing Lactic Acid Bacterium.
  • authors: Morinaga K, Kusada H, Watanabe M, Tamaki H
  • journal: Microbiol Resour Announc
  • DOI: 10.1128/MRA.00641-21
  • year: 2021

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
41132Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104344Collection of Institut Pasteur (CIP 104344)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
95671Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID3305.1
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG