Amycolatopsis orientalis M 43-05865, Pittinger M43-05865, CCTMLa 2924 | Type strain | DSM 40040, ATCC 19795, CBS 547.68, CCM 2778, IAM 14281, IFO 12360, IFO 12806, IMET 7510, IMET 7653, IMSNU 20058, ISP 5040, JCM 4235, JCM 4600, KCC S-0235, KCC S-0600, NBRC 12360, NBRC 12806, NIHJ 483, NRRL 2450, RIA 1074, BCRC 11615, CGMCC 4.1214, CIP 107113, HUT 6592, IFM 0528, KCCM 40595, KCTC 9412, NBIMCC 2297, NCIMB 12945, VKM Ac-866 | BacDiveID:13215

Strain identifier

BacDive ID: 13215

Type strain:

Species: Amycolatopsis orientalis

Strain Designation: M 43-05865, Pittinger M43-05865, CCTMLa 2924

Culture col. no.: DSM 40040, ATCC 19795, CBS 547.68, CCM 2778, IAM 14281, IFO 12360, IFO 12806, IMET 7510, IMET 7653, IMSNU 20058, ISP 5040, JCM 4235, JCM 4600, KCC S-0235, KCC S-0600, NBRC 12360, NBRC 12806, NIHJ 483, NRRL 2450, RIA 1074, BCRC 11615, CGMCC 4.1214, CIP 107113, HUT 6592, IFM 0528, KCCM 40595, KCTC 9412, NBIMCC 2297, NCIMB 12945, VKM Ac-866

Strain history: CIP <- 2001, CCM, Amycolatopsis orientalis subsp. orientalis <- R. E. Gordon <- E. B. Shirling <- R. C. Pittenger: strain M 43-05865

NCBI tax ID(s): 1125971 (strain), 31958 (species)

SI-ID 34716

ATCC 53630, NRRL 2450, ATCC 19795, IFO 12806, IMET 7653, DSM 40040, CCM 2778, IAM 14281, IFO 12360, IMET 7510, JCM 4235, JCM 4600, CCRC 11615, HUT 6592, NCIMB 12945, VKM Ac-866, NRRL B-2450, CBS 547.68, KCTC 9412, CIP 107113, NBRC 12806, NBRC 12360, BCRC 11615, NBIMCC 2297, CGMCC 4.1286, CGMCC 4.1214

Other strains from Amycolatopsis orientalis

A. orientalis N858, W-113/66, DSM 43388, CIP ...
A. orientalis F 719, F7-19, Kunz F-719, DSM 46075, ...
A. orientalis STH00299(ZIMET), HKI 0299
A. orientalis STH00300(ZIMET), HKI 0300
A. orientalis JCM 3128, NRRL B-3891

Section

Amycolatopsis orientalis M 43-05865 is an obligate aerobe, spore-forming, mesophilic human pathogen that builds an aerial mycelium and produces antibiotic compounds.

antibiotic compound production
Gram-positive
rod-shaped
spore-forming
human pathogen
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Amycolatopsis*
	Species	**Amycolatopsis orientalis**
	Full Scientific Name (LPSN)	Amycolatopsis orientalis (Pittenger and Brigham 1956) Lechevalier et al. 1986

	Synonym	"Streptomyces* orientalis"*
	Synonym	Nocardia orientalis

Information on morphological and physiological properties Morphology

[Ref.: #121436]	Gram stain	positive

[Ref.: #121436]	Cell shape	rod-shaped

[Ref.: #121436]	Motility	no

[Ref.: #19392]	Colony color	Yellow
[Ref.: #19392]	Incubation period	10-14 days
[Ref.: #19392]	Cultivation medium used	ISP 2

[Ref.: #19392]	Colony color	Beige
[Ref.: #19392]	Incubation period	10-14 days
[Ref.: #19392]	Cultivation medium used	ISP 3

[Ref.: #19392]	Colony color	Beige
[Ref.: #19392]	Incubation period	10-14 days
[Ref.: #19392]	Cultivation medium used	ISP 4

[Ref.: #19392]	Colony color	Beige
[Ref.: #19392]	Incubation period	10-14 days
[Ref.: #19392]	Cultivation medium used	ISP 5

[Ref.: #19392]	Colony color	Beige
[Ref.: #19392]	Incubation period	10-14 days
[Ref.: #19392]	Cultivation medium used	ISP 6

[Ref.: #19392]	Colony color	Beige
[Ref.: #19392]	Incubation period	10-14 days
[Ref.: #19392]	Cultivation medium used	ISP 7

[Ref.: #19392]	Multicellular complex forming ability	yes
[Ref.: #19392]	Multicellular complex name	Aerial Mycelium
[Ref.: #19392]	Multicellular complex color	White
[Ref.: #19392]	Medium Name (multicellularity)	ISP 2

[Ref.: #19392]	Multicellular complex forming ability	yes
[Ref.: #19392]	Multicellular complex name	Aerial Mycelium
[Ref.: #19392]	Multicellular complex color	White
[Ref.: #19392]	Medium Name (multicellularity)	ISP 3

[Ref.: #19392]	Multicellular complex forming ability	yes
[Ref.: #19392]	Multicellular complex name	Aerial Mycelium
[Ref.: #19392]	Multicellular complex color	White
[Ref.: #19392]	Medium Name (multicellularity)	ISP 4

[Ref.: #19392]	Multicellular complex forming ability	yes
[Ref.: #19392]	Multicellular complex name	Aerial Mycelium
[Ref.: #19392]	Multicellular complex color	White
[Ref.: #19392]	Medium Name (multicellularity)	ISP 5

[Ref.: #19392]	Multicellular complex forming ability	yes
[Ref.: #19392]	Multicellular complex name	Aerial Mycelium
[Ref.: #19392]	Multicellular complex color	White
[Ref.: #19392]	Medium Name (multicellularity)	ISP 6

[Ref.: #19392]	Multicellular complex forming ability	yes
[Ref.: #19392]	Multicellular complex name	Aerial Mycelium
[Ref.: #19392]	Multicellular complex color	White
[Ref.: #19392]	Medium Name (multicellularity)	ISP 7

Multimedia content

Caption:

Medium 65 28°C

Intellectual property rights:

© Leibniz-Institut DSMZ

Information on culture and growth conditions Culture and growth conditions

Culture medium

ISP 2

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 3

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 4

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 5

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 6

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 7

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

[Ref.: #121436]

Culture medium

CIP Medium 57

[Ref.: #121436]

Culture medium growth

[Ref.: #121436]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #9119]			growth	28 °C
[Ref.: #19392]			optimum	30 °C
[Ref.: #38186]			growth	30 °C
[Ref.: #67770]			growth	28 °C
[Ref.: #121436]			growth	10-41 °C
[Ref.: #121436]		no	growth	45 °C

[Ref.: #9119]	Temperature range	mesophilic
[Ref.: #19392]	Temperature range	mesophilic
[Ref.: #38186]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #121436]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #121436]

Oxygen tolerance

obligate aerobe

[Ref.: #19392]	Spore description	spore surface: smooth, Fragmentation: +
[Ref.: #19392]	Ability of spore formation	yes

[Ref.: #9119]	Name of produced compound	vancomycin
[Ref.: #20216]	Name of produced compound	Vancomycin

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #19392]		NaCl		maximum	5	%
[Ref.: #121436]		NaCl		growth	0-2	%
[Ref.: #121436]		NaCl	no	growth	4	%
[Ref.: #121436]		NaCl	no	growth	6	%
[Ref.: #121436]		NaCl	no	growth	8	%
[Ref.: #121436]		NaCl	no	growth	10	%

Observation

quinones: MK-9(H₄), MK-9(H₂)

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]		adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68371]		amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19392]		arabinose ChEBI	+
[Ref.: #68371]		arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]		arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68371]		cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19392]		cellulose ChEBI	-
[Ref.: #121436]		citrate ChEBI	-	carbon source
[Ref.: #68368]		citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68371]		D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-fructose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]		D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68371]		D-glucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]		D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68371]		D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]		D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68371]		D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]		D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68371]		D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]		decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68371]		erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121436]		esculin ChEBI	+	hydrolysis
[Ref.: #68371]		esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68369]		esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #19392]		fructose ChEBI	-
[Ref.: #68371]		galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]		gelatin ChEBI	+	hydrolysis	* from API 20NE, API 20E
[Ref.: #68371]		gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]		gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68371]		gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19392]		glucose ChEBI	+
[Ref.: #68371]		glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121436]		hippurate ChEBI	+	hydrolysis
[Ref.: #68371]		inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]		L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68371]		L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]		lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68369]		malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68371]		maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19392]		mannitol ChEBI	+
[Ref.: #68371]		melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19392]		myo-inositol ChEBI	+
[Ref.: #68371]		myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]		N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68371]		N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121436]		nitrate ChEBI	+	reduction
[Ref.: #121436]		nitrate ChEBI	-	respiration
[Ref.: #68369]		nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #121436]		nitrite ChEBI	-	reduction
[Ref.: #68368]		ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68371]		Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19392]		raffinose ChEBI	-
[Ref.: #68371]		raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19392]		rhamnose ChEBI	-
[Ref.: #68371]		ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19392]		sucrose ChEBI	-
[Ref.: #68371]		sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]		tryptophan ChEBI	-	energy source	* from API 20NE, API 20E
[Ref.: #68371]		turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]		urea ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68368]		urea ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68371]		xylitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19392]		xylose ChEBI	-
		Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #121436]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #121436]		indole ChEBI	no
[Ref.: #67770]		vancomycin ChEBI	yes
[Ref.: #68368]		acetoin ChEBI	no	* from API 20E
[Ref.: #68368]		hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68369]		indole ChEBI	no	* from API 20NE, API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68369]		indole ChEBI		-	* from API 20NE, API 20E

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #121436]		alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]		alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]		alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]		alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]		alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121436]		amylase	+
[Ref.: #68368]		arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #121436]		beta-galactosidase	+	3.2.1.23
[Ref.: #68368]		beta-galactosidase	-	3.2.1.23	* from API 20E
[Ref.: #68369]		beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #68382]		beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121436]		caseinase	+	3.4.21.50
[Ref.: #121436]		catalase	+	1.11.1.6
[Ref.: #68382]		cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #121436]		DNase	-
[Ref.: #68382]		esterase (C 4)	+		* from API zym
[Ref.: #68382]		esterase lipase (C 8)	+		* from API zym
[Ref.: #121436]		gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #121436]		gelatinase	+
[Ref.: #68369]		gelatinase	+		* from API 20NE, API 20E
[Ref.: #121436]		lecithinase	+
[Ref.: #121436]		lipase	-
[Ref.: #68382]		lipase (C 14)	-		* from API zym
[Ref.: #121436]		lysine decarboxylase	-	4.1.1.18
[Ref.: #68368]		lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]		N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #121436]		ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]		ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #121436]		oxidase	-
[Ref.: #121436]		phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68368]		tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #121436]		tween esterase	+
[Ref.: #121436]		urease	+	3.5.1.5
[Ref.: #68369]		urease	+	3.5.1.5	* from API 20NE
[Ref.: #68368]		urease	-	3.5.1.5	* from API 20E
		Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #19392]	258	-	+	-	-	+	-	-	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #9119]	25086	+	-	-	+/-	+	+	+	+	+	+	+	+	+	+/-	+	-	+	+	+/-	+	not determinedn.d.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #121436]	10843	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121436]	2008	not determinedn.d.	+	+	+	-	+	-	-	-	-	-	-	-	-	-	-	+	+	-	+	+	+	+	-	+	+	-	+	-	-	-	-	+	+	+	-	-	-	+	-	-	+	-	-	-	-	-	-	-	+	+	-	+	-	-	+	-	-	-	-	+	+	-	+	+	+	+	-	-	-	+	-	-	-	+	-	+	-	-	-	+	+	-	-	-	+	-	+	-	+	+	+	+	+	+	+	-	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19392]	344	not determinedn.d.	+	+	+	-	-	-	-	-	+	+	+	-	-	-	-	-	+	-	-
[Ref.: #9119]	14233	-	+	+	+	-	+	-	-	-	+/-	+/-	-	+/-	-	-	-	-	+	-	-
[Ref.: #121436]	10075	-	+	+	+	-	+	+	-	+	+	+	+	+	+	-	-	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #9119]	Sample type/isolated from	soil
[Ref.: #9119]	Geographic location (country and/or sea, region)	Asia

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Orient
[Ref.: #67770]	Country	Asia

[Ref.: #121436]	Sample type/isolated from	Environment, Soil
[Ref.: #121436]	Geographic location (country and/or sea, region)	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

Global distribution of 16S sequence AJ400711 (>99% sequence identity) for Amycolatopsis from Microbeatlas

Aquatic Samples	14
Soil Samples	213
Animal Samples	67
Plant Samples	25
Total Samples	319

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #9119]	Pathogenicity (human)	yes, in single cases
[Ref.: #9119]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19392]	Biosafety level	1	Risk group (German classification)
[Ref.: #121436]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Amycolatopsis orientalis gene for 16S ribosomal RNA, partial sequence	D86935	1467	ENA	31958 tax ID	*
[Ref.: #20218]	A.orientalis (ssp. orientalis DSM 40040) 16S ribosomal RNA (partial)	X76958	1444	ENA	31958 tax ID	*
[Ref.: #20218]	Amycolatopsis orientalis gene for 16S ribosomal RNA, partial sequence, strain: JCM 4235	D44036	120	ENA	31958 tax ID	*
[Ref.: #20218]	Amycolatopsis orientalis partial 16S rRNA gene, strain IMSNU 20058T	AJ400711	1458	ENA	31958 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Amycolatopsis orientalis DSM 40040 = KCTC 9412 KCTC 9412T	GCA_000478275	contig	GenBank	1125971 tax ID
[Ref.: #66792]	Amycolatopsis orientalis DSM 40040 = KCTC 9412	1125971.3	wgs	PATRIC	1125971 tax ID	*
[Ref.: #66792]	Amycolatopsis orientalis DSM 40040	2554235102	draft	JGI IMG/M	1125971 tax ID	*

[Ref.: #67770]	GC-content	66 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	69 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Amycolatopsis orientalis DSM 40040 = KCTC 9412 PATRIC: 1125971.3
[Ref.: #69480]	motile	no	93.163	no
[Ref.: #69480]	flagellated	no	97.005	no
[Ref.: #69480]	gram-positive	yes	84.444	no
[Ref.: #69480]	anaerobic	no	99.328	no
[Ref.: #69480]	aerobic	yes	94.291	no
[Ref.: #69480]	halophile	no	91.299	no
[Ref.: #69480]	spore-forming	yes	92.312	yes
[Ref.: #69480]	thermophile	no	97.47	yes
[Ref.: #69480]	glucose-util	yes	89.105	no
[Ref.: #69480]	glucose-ferment	no	93.845	yes

Availability in culture collections External links

Culture collection no.

DSM 40040, ATCC 19795, CBS 547.68, CCM 2778, IAM 14281, IFO 12360, IFO 12806, IMET 7510, IMET 7653, IMSNU 20058, ISP 5040, JCM 4235, JCM 4600, KCC S-0235, KCC S-0600, NBRC 12360, NBRC 12806, NIHJ 483, NRRL 2450, RIA 1074, BCRC 11615, CGMCC 4.1214, CIP 107113, HUT 6592, IFM 0528, KCCM 40595, KCTC 9412, NBIMCC 2297, NCIMB 12945, VKM Ac-866

SI-ID 34716

*

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Degradation of carbonyl sulfide by Actinomycetes and detection of clade D of beta-class carbonic anhydrase.	Ogawa T, Kato H, Higashide M, Nishimiya M, Katayama Y	FEMS Microbiol Lett	10.1093/femsle/fnw223	2016	*
Genetics	Genome Sequence of the Vancomycin-Producing Amycolatopsis orientalis subsp. orientalis Strain KCTC 9412T.	Jeong H, Sim YM, Kim HJ, Lee DW, Lim SK, Lee SJ	Genome Announc	10.1128/genomeA.00408-13	2013	*
Genetics	Nucleotide sequence of plasmid pA387 of Amycolatopsis benzoatilytica and construction of a conjugative shuttle vector.	Malhotra S, Majumdar S, Kumar M, Bhasin VK, Gartemann KH, Lal R	J Basic Microbiol	10.1002/jobm.200700326	2008	*
Phylogeny	Reclassification of Amycolatopsis orientalis DSM 43387 as Amycolatopsis benzoatilytica sp. nov.	Majumdar S, Prabhagaran SR, Shivaji S, Lal R	Int J Syst Evol Microbiol	10.1099/ijs.0.63766-0	2006	*
Phylogeny	Reclassification of Amycolatopsis orientalis subsp. lurida Lechevalier et al. 1986 as Amycolatopsis lurida sp. nov., comb. nov.	Stackebrandt E, Kroppenstedt RM, Wink J, Schumann P	Int J Syst Evol Microbiol	10.1099/ijs.0.02937-0	2004	*
Biotechnology	Vancomycin production is enhanced in chemostat culture with biomass-recycle.	McIntyre JJ, Bunch AW, Bull AT	Biotechnol Bioeng	10.1002/(sici)1097-0290(19990305)62:5<576::aid-bit9>3.0.co;2-p	1999	*
Biotechnology	Vancomycin production in batch and continuous culture.	McIntyre JJ, Bull AT, Bunch AW	Biotechnol Bioeng	10.1002/(SICI)1097-0290(19960220)49:4<412::AID-BIT8>3.0.CO;2-S	1996	*
Metabolism	Microbial de-mannosylation and mannosylation of teicoplanin derivatives.	Borghi A, Ferrari P, Gallo GG, Zanol M, Zerilli LF, Lancini GC	J Antibiot (Tokyo)	10.7164/antibiotics.44.1444	1991	*
Enzymology	Purification and characterization of an exo-beta-D-glucosaminidase, a novel type of enzyme, from Nocardia orientalis.	Nanjo F, Katsumi R, Sakai K	J Biol Chem	S0021-9258(19)38783-6	1990	*
Enzymology	Transglycosylation reaction of a chitinase purified from Nocardia orientalis.	Usui T, Hayashi Y, Nanjo F, Sakai K, Ishido Y	Biochim Biophys Acta	10.1016/0304-4165(87)90017-1	1987	*
	The Vancomycin biosynthetic pathway is induced in oxygen-limited Amycolatopsis orientalis (ATCC 19795) cultures that do not produce antibiotic.	Dunstan GH, Avignone-Rossa C, Langley D, Bushell ME	Enzyme Microb Technol	10.1016/s0141-0229(00)00238-6	2000	*

References

#9119

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40040

#19392

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20216

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38186 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68369

Automatically annotated from API 20NE .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#82423

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID34716.1 )

#121436 Collection of Institut Pasteur ; Curators of the CIP; CIP 107113
* These data were automatically processed and therefore are not curated

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