Name: Pseudomonas chlororaphis subsp. aureofaciens EIII.9.25.1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13158

Type strain:

Species: Pseudomonas chlororaphis subsp. aureofaciens

Strain Designation: EIII.9.25.1, L8

Culture col. no.: CCUG 712, CIP 103295, NCTC 10686, LMG 1245, NRRL B-1576, IFO 3521, DSM 6698, ATCC 13985, NCIMB 9030, JCM 20509, CCEB 518, IAM 12353, NBRC 3521

Strain history: CIP <- 1989, NCIMB, Pseudomonas aureofaciens <- Tech. Hoogeschool, Delft, The Netherlands: strain EIII.9.25.1 (= strain L8) <- A. Kluyver, Pseudomonas polychromogenes <- 1936, H. Bouman

NCBI tax ID(s): 1215095 (strain), 587851 (subspecies)

SI-ID 3935

LMG 1245, ATCC 13985, CCUG 712, IAM 12353, IFO 3521, NRRL B-1576, LMG 16909, DSM 6698, NCIMB 9030, NCTC 10686, CIP 103295, NCIB 9030, LMD 37.26, LMD 37.27, ICMP 13610, CECT 462, CCRC 11057, CFBP 2133, CCM 4973, NCCB 37026, NCCB 37027, NBRC 3521, BCRC 11057, KCTC 12349, NCIM 2026, CGMCC 1.3351, JCM 20509

Other strains from Pseudomonas chlororaphis subsp. aureofaciens

P. chlororaphis subsp. aureofaciens 305-STR-1-4, DSM 6508
P. chlororaphis subsp. aureofaciens RW71, DSM 11437, DSM 12972
P. chlororaphis subsp. aureofaciens DSM 50082, ATCC 13986, ICPB 2393, ...
P. chlororaphis subsp. aureofaciens DSM 50135, ATCC 17810, ICPB 2941-389, ...
P. chlororaphis subsp. aureofaciens DSM 50136, ATCC 17811, ICPB 2942-390, ...
P. chlororaphis subsp. aureofaciens DSM 50139, ATCC 17415, ICPB 2717-36, ...
P. chlororaphis subsp. aureofaciens PT02, DSM 29578

Section

Pseudomonas chlororaphis subsp. aureofaciens EIII.9.25.1 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from Clay suspended in kerosene for three weeks.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas chlororaphis subsp. aureofaciens**
	Full Scientific Name (LPSN)	Pseudomonas chlororaphis subsp. aureofaciens (Kluyver 1956) Peix et al. 2007

Synonym

Pseudomonas aureofaciens

Information on morphological and physiological properties Morphology

[Ref.: #118246]	Gram stain	negative

[Ref.: #118246]	Cell shape	rod-shaped

[Ref.: #118246]	Motility	yes

[Ref.: #118246]	Pigment production	no
[Ref.: #118246]	Pigment name	Pyocyanin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2775]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #2775]

Culture medium growth

[Ref.: #2775]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2775]

Culture medium composition

expand / minimize

[Ref.: #38123]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #38123]

Culture medium growth

[Ref.: #38123]

Culture medium composition

expand / minimize

[Ref.: #118246]

Culture medium

CIP Medium 3

[Ref.: #118246]

Culture medium growth

[Ref.: #118246]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2775]		growth	25 °C
[Ref.: #38123]		growth	30 °C
[Ref.: #44248]		growth	28 °C
[Ref.: #67770]		growth	25 °C
[Ref.: #118246]		growth	5-37 °C
[Ref.: #118246]	no	growth	41 °C

[Ref.: #2775]	Temperature range	mesophilic
[Ref.: #38123]	Temperature range	mesophilic
[Ref.: #44248]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #118246]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44248]	Oxygen tolerance	aerobe
[Ref.: #118246]	Oxygen tolerance	obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #118246]	NaCl		growth	0-6	%
[Ref.: #118246]	NaCl	no	growth	8	%
[Ref.: #118246]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #118246]	citrate ChEBI	+	carbon source
[Ref.: #118246]	esculin ChEBI	-	hydrolysis
[Ref.: #118246]	nitrate ChEBI	+	reduction
[Ref.: #118246]	nitrate ChEBI	-	respiration
[Ref.: #118246]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #118246]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #118246]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #118246]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #118246]	amylase	-
[Ref.: #118246]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118246]	caseinase	+	3.4.21.50
[Ref.: #118246]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #118246]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #118246]	gelatinase	+/-
[Ref.: #118246]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #118246]	lipase	+
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #118246]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118246]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #118246]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118246]	tryptophan deaminase	-
[Ref.: #118246]	tween esterase	+
[Ref.: #118246]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #44248]

Fatty acid	Percentage	ECL
C16:0	28.5	16
C17:0 CYCLO	18.5	16.888
C16:1 ω7c	15.6	15.819
C18:1 ω7c /12t/9t	7.9	17.824
C10:0 3OH	6.7	11.423
C12:0 3OH	6.2	13.455
C12:0 2OH	5.7	13.178
C12:0	2.2	12
C14:0 3OH/C16:1 ISO I	1.2	15.485
C12:1 3OH	1.1	13.289
Unidentified	0.7	15.265
Unidentified	0.7	12.342
C18:1 ω9c	0.7	17.769
C18:2 ω6,9c/C18:0 ANTE	0.7	17.724
Unidentified	0.7	10.46
unknown 12.486	0.6	12.486
C14:0	0.6	14
Unidentified	0.5	9.969
C15:0	0.4	15
C19:0 CYCLO ω8c	0.4	18.9
Unidentified	0.3	11.183

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #118246]	381	not determinedn.d.	+	+	+	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	+	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	+	+	-	-	-	+	+	+	+	-	+	-	-	+	+	+	+	+	+	+	+	+	-	-	+	-	-	-	+	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+	+	+	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118246]	7480	-	+	+	+	+	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #44248]	Sample type/isolated from	Clay suspended in kerosene for three weeks
[Ref.: #44248]	Sampling date	1937
[Ref.: #44248]	Geographic location (country and/or sea, region)	Maas river
[Ref.: #44248]	Country	Netherlands
[Ref.: #44248]	Country ISO 3 Code	NLD
[Ref.: #44248]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Clay of Maas River by kerosene enrichment

[Ref.: #118246]	Sample type/isolated from	Environment, Maas River Clay suspended in kerosene
* marker position based on {}

Isolation sources categories

#Engineered	#Laboratory	#Lab enrichment
#Engineered	#Contamination	#Oil (Fuel)
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FJ652608 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	1
Soil Samples	3
Animal Samples	6
Plant Samples	3
Total Samples	13

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2775]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118246]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #2775]	Pseudomonas chlororaphis subsp. aureofaciens strain DSM 6698 16S ribosomal RNA gene, complete sequence	AY509898	1530	ENA	587851 tax ID
[Ref.: #67770]	Pseudomonas chlororaphis gene for 16S rRNA, partial sequence	D84008	1408	ENA	587753 tax ID
[Ref.: #20218]	Pseudomonas chlororaphis subsp. aureofaciens strain ATCC 13985 16S ribosomal RNA gene, partial sequence	AF094722	1458	ENA	587851 tax ID	*
[Ref.: #20218]	Pseudomonas chlororaphis strain ATCC 13985 16S ribosomal RNA (rrsA) gene, partial sequence	FJ652608	1459	ENA	587753 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Pseudomonas chlororaphis subsp. aureofaciens NBRC 3521	GCA_000813225	contig	GenBank	1215095 tax ID
[Ref.: #67770]	Pseudomonas chlororaphis subsp. aureofaciens LMG 1245	GCA_001269575	contig	GenBank	587851 tax ID
[Ref.: #67770]	Pseudomonas chlororaphis NCTC10686	GCA_900455495	contig	GenBank	587753 tax ID
[Ref.: #66792]	Pseudomonas chlororaphis subsp. aureofaciens CCUG 712	GCA_008801625	contig	GenBank	587851 tax ID	*
[Ref.: #66792]	Pseudomonas chlororaphis strain ATCC 13985	587753.24	complete	PATRIC	587753 tax ID	*
[Ref.: #66792]	Pseudomonas chlororaphis strain NCTC10686	587753.54	wgs	PATRIC	587753 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas chlororaphis subsp. aureofaciens DSM 6698 NCBI: GCA_003851905.1
[Ref.: #69480]	motile	yes	92.177	no
[Ref.: #69480]	flagellated	yes	79.564	no
[Ref.: #69480]	gram-positive	no	98.307	no
[Ref.: #69480]	anaerobic	no	98.338	yes
[Ref.: #69480]	aerobic	yes	94.701	no
[Ref.: #69480]	halophile	no	92.104	no
[Ref.: #69480]	spore-forming	no	93.605	no
[Ref.: #69480]	thermophile	no	99.833	yes
[Ref.: #69480]	glucose-util	yes	95.131	no
[Ref.: #69480]	glucose-ferment	no	91.077	no

Availability in culture collections External links

[Ref.: #2775]

Culture collection no.

CCUG 712, CIP 103295, NCTC 10686, LMG 1245, NRRL B-1576, IFO 3521, DSM 6698, ATCC 13985, NCIMB 9030, JCM 20509, CCEB 518, IAM 12353, NBRC 3521

[Ref.: #82366]

SI-ID 3935

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	A simple and rapid GC/MS method for the simultaneous determination of gaseous metabolites.	Isobe K, Koba K, Ueda S, Senoo K, Harayama S, Suwa Y	J Microbiol Methods	10.1016/j.mimet.2010.10.009	2010	*
Phylogeny	Reclassification of Pseudomonas aurantiaca as a synonym of Pseudomonas chlororaphis and proposal of three subspecies, P. chlororaphis subsp. chlororaphis subsp. nov., P. chlororaphis subsp. aureofaciens subsp. nov., comb. nov. and P. chlororaphis subsp. aurantiaca subsp. nov., comb. nov.	Peix A, Valverde A, Rivas R, Igual JM, Ramirez-Bahena MH, Mateos PF, Santa-Regina I, Rodriguez-Barrueco C, Martinez-Molina E, Velazquez E	Int J Syst Evol Microbiol	10.1099/ijs.0.64621-0	2007	*

References

#2775

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6698

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38123 ; Curators of the CIP;
#44248 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 712

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#82366

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID3935.1 )

#118246 Collection of Institut Pasteur ; Curators of the CIP; CIP 103295
* These data were automatically processed and therefore are not curated

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Pseudomonas chlororaphis subsp. aureofaciens DSM 6698

Change proposal

Change proposal