Name: Pseudomonas chlororaphis subsp. aurantiaca DSM 19603
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13159

Type strain:

Species: Pseudomonas chlororaphis subsp. aurantiaca

Culture col. no.: DSM 19603, ATCC 33663, CIP 106718, LMG 21630, NCIMB 10068

Strain history: CIP <- 2000, NCIMB <- CCM

NCBI tax ID(s): 86192 (subspecies)

SI-ID 323069

ATCC 33663, NCIB 10068, CIP 106718, NCIMB 10068, VKM B-876, LMG 21630, ICMP 6003, BCRC 15821, CCRC 15821, CFBP 4369, DSM 19603

Other strains from Pseudomonas chlororaphis subsp. aurantiaca

P. chlororaphis subsp. aurantiaca CCUG 3377 A
P. chlororaphis subsp. aurantiaca CCUG 3377 B

Section

Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 is an obligate aerobe, mesophilic, Gram-negative bacterium of the family Pseudomonadaceae.

Gram-negative
motile
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas chlororaphis subsp. aurantiaca**
	Full Scientific Name (LPSN)	Pseudomonas chlororaphis subsp. aurantiaca (Nakhimovskaya 1948) Peix et al. 2007

Synonym

Pseudomonas aurantiaca

Information on morphological and physiological properties Morphology

[Ref.: #118247]	Gram stain	negative

[Ref.: #118247]	Cell shape	rod-shaped

[Ref.: #118247]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8206]

Culture medium

REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)

[Ref.: #8206]

Culture medium growth

[Ref.: #8206]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8206]

Culture medium composition

expand / minimize

[Ref.: #37249]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37249]

Culture medium growth

[Ref.: #37249]

Culture medium composition

expand / minimize

[Ref.: #118247]

Culture medium

CIP Medium 72

[Ref.: #118247]

Culture medium growth

[Ref.: #118247]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #8206]		growth	28 °C
[Ref.: #37249]		growth	25 °C
[Ref.: #118247]		growth	5-37 °C
[Ref.: #118247]	no	growth	41 °C

[Ref.: #8206]	Temperature range	mesophilic
[Ref.: #37249]	Temperature range	mesophilic
[Ref.: #118247]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #118247]

Oxygen tolerance

obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #118247]	NaCl		growth	0-6	%
[Ref.: #118247]	NaCl	no	growth	8	%
[Ref.: #118247]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #118247]	citrate ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #118247]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #118247]	nitrate ChEBI	+	reduction
[Ref.: #118247]	nitrate ChEBI	-	respiration
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #118247]	nitrite ChEBI	-	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #118247]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #118247]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #118247]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #118247]	amylase	-
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #118247]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118247]	caseinase	+	3.4.21.50
[Ref.: #118247]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #118247]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #118247]	gelatinase	+
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #118247]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #118247]	lipase	+
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #118247]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118247]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #118247]	oxidase	+
[Ref.: #118247]	protease	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #118247]	tryptophan deaminase	-
[Ref.: #118247]	tween esterase	+
[Ref.: #118247]	urease	+	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #8206]	22898	-	-	-	+	-	-	+	-	+	+	+	+	+	-	+	+	-	+	+	+	+

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #118247]	1792	not determinedn.d.	+	+	+	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	+	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	+	+	+	-	+	-	-	+	-	-	+	+	+	+	+	+	-	-	+	-	-	-	+	+	+	+	+	+	+	-	+	+	+	-	-	+	-	-	+	+	+	+	+	+	+	+	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118247]	9749	-	+	+	+	+	+	+	-	+	-	+	+	-	-	-	-	-	-	-	-

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8206]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118247]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8206]	Pseudomonas chlororaphis subsp. aurantiaca gene for 16S rRNA, strain: ATCC 33663	AB021412	1504	ENA	86192 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas chlororaphis subsp. aurantiaca DSM 19603	GCA_003851835	complete	GenBank	86192 tax ID	*
[Ref.: #66792]	Pseudomonas chlororaphis LMG 21630	GCA_900104985	chromosome	GenBank	587753 tax ID	*
[Ref.: #66792]	Pseudomonas chlororaphis strain LMG 21630	587753.25	complete	PATRIC	587753 tax ID	*
[Ref.: #66792]	Pseudomonas chlororaphis subsp. aurantiaca strain DSM 19603	86192.21	complete	PATRIC	86192 tax ID	*
[Ref.: #66792]	Pseudomonas chlororaphis aurantiaca DSM 19603	2908486269	complete	JGI IMG/M	86192 tax ID	*
[Ref.: #66792]	Pseudomonas chlororaphis aurantiaca LMG 21630	2636416019	draft	JGI IMG/M	86192 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 NCBI: GCA_003851835.1
[Ref.: #69480]	motile	yes	92.26	no
[Ref.: #69480]	flagellated	yes	83.307	no
[Ref.: #69480]	gram-positive	no	98.268	no
[Ref.: #69480]	anaerobic	no	98.649	no
[Ref.: #69480]	aerobic	yes	95.335	no
[Ref.: #69480]	halophile	no	89.696	no
[Ref.: #69480]	spore-forming	no	94.044	no
[Ref.: #69480]	thermophile	no	99.855	yes
[Ref.: #69480]	glucose-util	yes	94.994	yes
[Ref.: #69480]	glucose-ferment	no	90.955	yes

Availability in culture collections External links

[Ref.: #8206]

Culture collection no.

DSM 19603, ATCC 33663, CIP 106718, LMG 21630, NCIMB 10068

[Ref.: #82367]

SI-ID 323069

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Biotechnology	Enhanced co-production of medium-chain-length polyhydroxyalkanoates and phenazines from crude glycerol by high cell density cultivation of Pseudomonas chlororaphis in membrane bioreactor.	Aloui H, Khomlaem C, Torres CAV, Freitas F, Reis MAM, Kim BS	Int J Biol Macromol	10.1016/j.ijbiomac.2022.05.089	2022	*
Enzymology	Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization.	Pereira JR, Araujo D, Freitas P, Marques AC, Alves VD, Sevrin C, Grandfils C, Fortunato E, Reis MAM, Freitas F	Int J Biol Macromol	10.1016/j.ijbiomac.2020.11.162	2020	*
Metabolism	Control of pyrimidine nucleotide formation in Pseudomonas aurantiaca.	Domakonda A, West TP	Arch Microbiol	10.1007/s00203-020-01842-x	2020	*
Metabolism	Pseudomonas chlororaphis as a multiproduct platform: Conversion of glycerol into high-value biopolymers and phenazines.	de Meneses L, Pereira JR, Sevrin C, Grandfils C, Paiva A, Reis MAM, Freitas F	N Biotechnol	10.1016/j.nbt.2019.10.002	2019	*
Pathogenicity	Demonstration of the adhesive properties of the medium-chain-length polyhydroxyalkanoate produced by Pseudomonas chlororaphis subsp. aurantiaca from glycerol.	Pereira JR, Araujo D, Marques AC, Neves LA, Grandfils C, Sevrin C, Alves VD, Fortunato E, Reis MAM, Freitas F	Int J Biol Macromol	10.1016/j.ijbiomac.2018.09.064	2018	*
Phylogeny	Luteibacter rhizovicinus MIMR1 promotes root development in barley (Hordeum vulgare L.) under laboratory conditions.	Guglielmetti S, Basilico R, Taverniti V, Arioli S, Piagnani C, Bernacchi A	World J Microbiol Biotechnol	10.1007/s11274-013-1365-6	2013	*
Phylogeny	Reclassification of Pseudomonas aurantiaca as a synonym of Pseudomonas chlororaphis and proposal of three subspecies, P. chlororaphis subsp. chlororaphis subsp. nov., P. chlororaphis subsp. aureofaciens subsp. nov., comb. nov. and P. chlororaphis subsp. aurantiaca subsp. nov., comb. nov.	Peix A, Valverde A, Rivas R, Igual JM, Ramirez-Bahena MH, Mateos PF, Santa-Regina I, Rodriguez-Barrueco C, Martinez-Molina E, Velazquez E	Int J Syst Evol Microbiol	10.1099/ijs.0.64621-0	2007	*

References

#8206

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19603

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#37249 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#82367

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID323069.1 )

#118247 Collection of Institut Pasteur ; Curators of the CIP; CIP 106718
* These data were automatically processed and therefore are not curated

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