Name: Pseudomonas stutzeri JM300
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13001

Type strain:

Species: Pseudomonas stutzeri

Strain Designation: JM300

Culture col. no.: DSM 10701, JCM 21571, IAM 15110

Strain history: IAM 15110 <-- DSM 10701 <-- E. Moore <-- G. J. Stewart <-- B. A. Bryan.

NCBI tax ID(s): 1123519 (strain), 203192 (species), 316 (species)

SI-ID 48860

DSM 10701, JCM 21571, IAM 15110

Other strains from Pseudomonas stutzeri

Section

Pseudomonas stutzeri JM300 is a mesophilic, motile human pathogen that was isolated from anaerobic enrichment from soil.

motile
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas stutzeri**
	Full Scientific Name (LPSN)	Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946 (Approved Lists 1980)

	Synonym	Stutzerimonas chloritidismutans
	Synonym	"Pseudomonas* perfectomarinus"*
	Synonym	"Bacterium* stutzeri"*
	Synonym	Pseudomonas kunmingensis
	Synonym	Pseudomonas chloritidismutans
	Synonym	Stutzerimonas kunmingensis
	Synonym	Stutzerimonas stutzeri
	Synonym	Stutzerimonas perfectomarina
	Synonym	Pseudomonas perfectomarina

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #69480]	Motility	yes Confidence in %97.794

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4044]

Culture medium

TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)

[Ref.: #4044]

Culture medium growth

[Ref.: #4044]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4044]

Culture medium composition

expand / minimize

[Ref.: #4044]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #4044]

Culture medium growth

[Ref.: #4044]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4044]

Culture medium composition

expand / minimize

[Ref.: #4044]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #4044]

Culture medium growth

[Ref.: #4044]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4044]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #4044]	growth	28 °C
[Ref.: #4044]	growth	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #4044]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.991

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #4044]	24866	+	-	-	-	-	-	-	-	+	-	+	+	-	+	+	+	-	+	+	-	+
[Ref.: #4044]	23070	+	-	-	-	-	-	-	-	+	-	+	+	-	+	+	+	-	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4044]	Sample type/isolated from	anaerobic enrichment from soil
[Ref.: #4044]	Geographic location (country and/or sea, region)	California
[Ref.: #4044]	Country	USA
[Ref.: #4044]	Country ISO 3 Code	USA
[Ref.: #4044]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	CA
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered	#Laboratory	#Lab enrichment
#Environmental	#Terrestrial	#Soil
#Condition	#Anoxic (anaerobic)	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4044]	Pathogenicity (human)	yes, in single cases
[Ref.: #4044]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Pseudomonas stutzeri 16S rRNA gene, partial sequence	U58661	1344	ENA	316 tax ID	*
[Ref.: #20218]	P.stutzeri 16S ribosomal RNA gene	X98607	1492	ENA	316 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Stutzerimonas stutzeri DSM 10701	GCA_000279165	complete	GenBank	1123519 tax ID	*
[Ref.: #66792]	Pseudomonas stutzeri DSM 10701	1123519.7	complete	PATRIC	1123519 tax ID	*
[Ref.: #66792]	Pseudomonas stutzeri JM3000, DSM 10701	2519899778	complete	JGI IMG/M	1123519 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pseudomonas stutzeri DSM 10701 DSM 10701 NCBI: GCA_000279165.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas stutzeri DSM 10701 DSM 10701 NCBI: GCA_000279165.1
[Ref.: #69480]	motile	yes	89.935	no
[Ref.: #69480]	flagellated	yes	87.811	no
[Ref.: #69480]	gram-positive	no	98.898	no
[Ref.: #69480]	anaerobic	no	97.586	no
[Ref.: #69480]	aerobic	yes	86.89	no
[Ref.: #69480]	halophile	no	87.923	no
[Ref.: #69480]	spore-forming	no	95.726	no
[Ref.: #69480]	glucose-util	yes	86.701	yes
[Ref.: #69480]	thermophile	no	99.55	yes
[Ref.: #69480]	glucose-ferment	no	92.371	no

Availability in culture collections External links

[Ref.: #4044]

Culture collection no.

DSM 10701, JCM 21571, IAM 15110

[Ref.: #82211]

SI-ID 48860

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Expression of nirK and nirS genes in two strains of Pseudomonas stutzeri harbouring both types of NO-forming nitrite reductases.	Wittorf L, Jones CM, Bonilla-Rosso G, Hallin S	Res Microbiol	10.1016/j.resmic.2018.04.010	2018	*
Genetics	Genome sequence of Pseudomonas stutzeri strain JM300 (DSM 10701), a soil isolate and model organism for natural transformation.	Busquets A, Pena A, Gomila M, Bosch R, Nogales B, Garcia-Valdes E, Lalucat J, Bennasar A	J Bacteriol	10.1128/JB.01257-12	2012	*
Metabolism	Posttranslational modification of myxobacterial carrier protein domains in Pseudomonas sp. by an intrinsic phosphopantetheinyl transferase.	Gross F, Gottschalk D, Muller R	Appl Microbiol Biotechnol	10.1007/s00253-004-1836-7	2005	*
Metabolism	DNA restriction is a barrier to natural transformation in Pseudomonas stutzeri JM300.	Berndt C, Meier P, Wackernagel W	Microbiology (Reading)	10.1099/mic.0.26033-0	2003	*
Enzymology	Cyclohexadienyl dehydrogenase from Pseudomonas stutzeri exemplifies a widespread type of tyrosine-pathway dehydrogenase in the TyrA protein family.	Xie G, Bonner CA, Jensen RA	Comp Biochem Physiol C Toxicol Pharmacol	10.1016/s0742-8413(99)00090-0	2000	*
Genetics	The potential for intraspecific horizontal gene exchange by natural genetic transformation: sexual isolation among genomovars of Pseudomonas stutzeri.	Lorenz MG, Sikorski J	Microbiology (Reading)	10.1099/00221287-146-12-3081	2000	*
Genetics	Type IV pilus genes pilA and pilC of Pseudomonas stutzeri are required for natural genetic transformation, and pilA can be replaced by corresponding genes from nontransformable species.	Graupner S, Frey V, Hashemi R, Lorenz MG, Brandes G, Wackernagel W	J Bacteriol	10.1128/JB.182.8.2184-2190.2000	2000	*
Metabolism	Natural genetic transformation of Pseudomonas stutzeri in a non-sterile soil.	Sikorski J, Graupner S, Lorenz MG, Wackernagel W	Microbiology (Reading)	10.1099/00221287-144-2-569	1998	*
Phylogeny	The naturally transformable marine bacterium WJT-1C formally identified as "Vibrio" is a pseudomonad.	Frischer ME, Williams HG, Bennison B, Drake GR, Balkwill DL, Paul JH	Curr Microbiol	10.1007/s002849900115	1996	*
Enzymology	Structure and regulation of the carAB operon in Pseudomonas aeruginosa and Pseudomonas stutzeri: no untranslated region exists.	Kwon DH, Lu CD, Walthall DA, Brown TM, Houghton JE, Abdelal AT	J Bacteriol	10.1128/jb.176.9.2532-2542.1994	1994	*
Enzymology	Isolation, sequencing and mutational analysis of a gene cluster involved in nitrite reduction in Paracoccus denitrificans.	de Boer AP, Reijnders WN, Kuenen JG, Stouthamer AH, van Spanning RJ	Antonie Van Leeuwenhoek	10.1007/BF00871635	1994	*
Enzymology	Characterization of the structural gene encoding a copper-containing nitrite reductase and homology of this gene to DNA of other denitrifiers.	Ye RW, Fries MR, Bezborodnikov SG, Averill BA, Tiedje JM	Appl Environ Microbiol	10.1128/aem.59.1.250-254.1993	1993	*
Enzymology	Isolation and characterization of a nitrite reductase gene and its use as a probe for denitrifying bacteria.	Smith GB, Tiedje JM	Appl Environ Microbiol	10.1128/aem.58.1.376-384.1992	1992	*
Enzymology	Purification of cytochrome cd1 nitrite reductase from Pseudomonas stutzeri JM300 and reconstitution with native and synthetic heme d1.	Weeg-Aerssens E, Wu WS, Ye RW, Tiedje JM, Chang CK	J Biol Chem	S0021-9258(20)89474-5	1991	*
Metabolism	Exchange of chromosomal markers by natural transformation between the soil isolate, Pseudomonas stutzeri JM300, and the marine isolate, Pseudomonas stutzeri strain ZoBell.	Stewart GJ, Sinigalliano CD	Antonie Van Leeuwenhoek	10.1007/BF00582115	1991	*
Metabolism	Steady-state nitric oxide concentrations during denitrification.	Goretski J, Zafiriou OC, Hollocher TC	J Biol Chem	S0021-9258(19)38430-3	1990	*
Phylogeny	Genome organization of Pseudomonas stutzeri and resulting taxonomic and evolutionary considerations.	Ginard M, Lalucat J, Tummler B, Romling U	Int J Syst Bacteriol	10.1099/00207713-47-1-132	1997	*
	Impact of mutS inactivation on foreign DNA acquisition by natural transformation in Pseudomonas stutzeri.	Meier P, Wackernagel W	J Bacteriol	10.1128/JB.187.1.143-154.2005	2005	*
	Inducible cell lysis system for the study of natural transformation and environmental fate of DNA released by cell death.	Kloos DU, Stratz M, Guttler A, Steffan RJ, Timmis KN	J Bacteriol	10.1128/jb.176.23.7352-7361.1994	1994	*

References

#4044

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10701

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#82211

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID48860.1 )

* These data were automatically processed and therefore are not curated

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Pseudomonas stutzeri DSM 10701 DSM 10701

Pseudomonas stutzeri DSM 10701 DSM 10701

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