Stutzerimonas chloritidismutans (DSM 13592, KCTC 12116, CCUG 51509)

Name: Stutzerimonas chloritidismutans (DSM 13592, KCTC 12116, CCUG 51509)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13003

Type strain

Strain Designation AW-1

Culture col. no. DSM 13592 KCTC 12116 CCUG 51509 ATCC BAA-443 AW-1 4 more

NCBI tax ID(s) 203192

Special Collections DSMZ CCUG JCM KCTC CIP

History DSMZ <- A. Wolterink, Univ. Wageningen; AW-1 JCM <-- IAM 15183 <-- KCTC 12116 <-- DSM 13592 <-- A. Wolterink AW-1. CIP <- 2003, A. Wolterink, Wageningen Univ., Wageningen, The Netherlands, Pseudomonas chloritidismutans: strain AW-1

Links

Stutzerimonas chloritidismutans AW-1 is an aerobe, Gram-negative, motile bacterium that was isolated from airlift reactor.

Gram-negative motile rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 100885

ATCC BAA-443,DSM 13592,KCTC 12116,LMG 23064,CCUG 51509,NBRC 102227,IAM 15183,JCM 21638,CIP 107870

@ref 20215

Last LPSN update 2026-05-29 08:27:56

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Stutzerimonas

Species Stutzerimonas chloritidismutans

Full scientific name Stutzerimonas chloritidismutans (Wolterink et al. 2002) Lalucat et al. 2022

Synonyms (3)

Stutzerimonas kunmingensis
Pseudomonas kunmingensis
Pseudomonas chloritidismutans

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
123337	negative	rod-shaped
125438	negative			97

Pigmentation

@ref	Production	Name
123337		Pyocyanin

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5100	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
5100	PSEUDOMONAS CHLOROTIDISMUTANS MEDIUM (DSMZ Medium 944)	Medium recipe at MediaDive	Name: PSEUDOMONAS CHLOROTIDISMUTANS MEDIUM (DSMZ Medium 944) Composition: NaHCO3 3.73 g/l Na-acetate x 3 H2O 2.72 g/l NaClO3 1.06 g/l Na2HPO4 0.53 g/l Na2S x 9 H2O 0.5 g/l NH4HCO3 0.44 g/l KH2PO4 0.41 g/l CaCl2 0.11 g/l MgCl2 0.1 g/l NaOH 0.005 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l alpha-lipoic acid 5e-05 g/l Riboflavin 5e-05 g/l Pantothenic acid 5e-05 g/l Vitamin B12 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l Na2WO4 x 2 H2O 3e-05 g/l Na2SeO3 x 5 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l Nicotinic acid 2.5e-05 g/l Nicotine amide 2.5e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Biotin 2e-05 g/l Folic acid 2e-05 g/l Pyridoxamine hydrochloride 1e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
5100	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
34277	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123337	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
5100	positive	growth	30
5100	positive	growth	28
34277	positive	growth	30
59220	positive	growth	30
67770	positive	growth	30
123337	positive	growth	25-37
123337	negative	growth	5
123337	negative	growth	10
123337	negative	growth	41

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
5100	aerobe
5100	anaerobe
59220	aerobe
123337	obligate aerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
123337	NaCl	positive	growth	0-6 %
123337	NaCl		growth	8 %
123337	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
123337	16947 ChEBI	citrate	-	carbon source
123337	4853 ChEBI	esculin	-	hydrolysis
123337	17632 ChEBI	nitrate	+	reduction
123337	17632 ChEBI	nitrate	-	respiration
123337	16301 ChEBI	nitrite	+	reduction

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
123337	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123337	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
123337	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123337	amylase	+
68382	beta-galactosidase	-	3.2.1.23	from API zym
123337	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123337	caseinase	-	3.4.21.50
123337	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
123337	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
123337	gelatinase	-
123337	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
123337	lipase	-
68382	lipase (C 14)	-		from API zym
123337	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123337	ornithine decarboxylase	-	4.1.1.17
123337	oxidase	+
123337	protease	+
68382	trypsin	-	3.4.21.4	from API zym
123337	tryptophan deaminase	-
123337	tween esterase	+
123337	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123337	-	+	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
123337	not determinedn.d.	+	+	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	+	+	-	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-	-	-	-	-	+	-	+	+	+	-	+	+	+	+	-	-	+	+	+	+	-	+	+	+	+	-	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1
#Engineered

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
5100	airlift reactor	Botlek, area, Rotterdam	Netherlands	NLD	Europe
59220	Airlift reactor		Netherlands	NLD	Europe
67770	Air lift reactor
123337	Environment, Sludge		Netherlands	NLD	Europe	1999

Taxonmaps

69479

Global distribution of 16S sequence AY017341 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	64
Soil Samples	62
Animal Samples	92
Plant Samples	25
Total Samples	243

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
5100	yes, in single cases	1	Risk group (German classification) According to TRBA 466
123337		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	PseChl assembly for Stutzerimonas chloritidismutans AW-1	contig	GCA_000495915	1263865.4	2568526533	1263865	58.54

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
67770	Pseudomonas chloritidismutans 16S ribosomal RNA gene, partial sequence	AY017341	1501		203192
124043	Pseudomonas stutzeri gene for 16S rRNA, partial sequence, strain: NBRC 102227.	AB681741	1462		316

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Stutzerimonas chloritidismutans AW-1 AW-1
NCBI: GCA_000495915.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.00	no
125438	anaerobic	anaerobicⓘ	no	97.29	no
125438	aerobic	aerobicⓘ	yes	89.53	no
125438	spore-forming	spore-formingⓘ	no	86.80	no
125438	thermophilic	thermophileⓘ	no	98.33	no
125438	flagellated	motile2+ⓘ	yes	87.48	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Comparative Genomic Analysis of Three Pseudomonas Species Isolated from the Eastern Oyster (Crassostrea virginica) Tissues, Mantle Fluid, and the Overlying Estuarine Water Column.	Pathak A, Stothard P, Chauhan A.	Microorganisms	10.3390/microorganisms9030490	2021
Enzymology	Characterization of the chlorate reductase from Pseudomonas chloritidismutans.	Wolterink AF, Schiltz E, Hagedoorn PL, Hagen WR, Kengen SW, Stams AJ.	J Bacteriol	10.1128/jb.185.10.3210-3213.2003	2003
	Biology of Pseudomonas stutzeri.	Lalucat J, Bennasar A, Bosch R, Garcia-Valdes E, Palleroni NJ.	Microbiol Mol Biol Rev	10.1128/mmbr.00047-05	2006
Metabolism	Concurrent Haloalkanoate Degradation and Chlorate Reduction by Pseudomonas chloritidismutans AW-1(T).	Peng P, Zheng Y, Koehorst JJ, Schaap PJ, Stams AJM, Smidt H, Atashgahi S	Appl Environ Microbiol	10.1128/AEM.00325-17	2017
Metabolism	Genome and proteome analysis of Pseudomonas chloritidismutans AW-1(T) that grows on n-decane with chlorate or oxygen as electron acceptor.	Mehboob F, Oosterkamp MJ, Koehorst JJ, Farrakh S, Veuskens T, Plugge CM, Boeren S, de Vos WM, Schraa G, Stams AJ, Schaap PJ	Environ Microbiol	10.1111/1462-2920.12880	2015
Metabolism	Structure and evolution of chlorate reduction composite transposons.	Clark IC, Melnyk RA, Engelbrektson A, Coates JD	mBio	10.1128/mBio.00379-13	2013
Metabolism	Growth of Pseudomonas chloritidismutans AW-1(T) on n-alkanes with chlorate as electron acceptor.	Mehboob F, Junca H, Schraa G, Stams AJ	Appl Microbiol Biotechnol	10.1007/s00253-009-1985-9	2009
Genetics	Genotype versus phenotype in the circumscription of bacterial species: the case of Pseudomonas stutzeri and Pseudomonas chloritidismutans.	Cladera AM, Garcia-Valdes E, Lalucat J	Arch Microbiol	10.1007/s00203-005-0052-x	2005
Genetics	Genome-Based Taxonomy of the Genus Stutzerimonas and Proposal of S. frequens sp. nov. and S. degradans sp. nov. and Emended Descriptions of S. perfectomarina and S. chloritidismutans.	Gomila M, Mulet M, Garcia-Valdes E, Lalucat J.	Microorganisms	10.3390/microorganisms10071363	2022
Phylogeny	Dechloromonas hortensis sp. nov. and strain ASK-1, two novel (per)chlorate-reducing bacteria, and taxonomic description of strain GR-1.	Wolterink A, Kim S, Muusse M, Kim IS, Roholl PJM, van Ginkel CG, Stams AJM, Kengen SWM	Int J Syst Evol Microbiol	10.1099/ijs.0.63404-0	2005
Phylogeny	Pseudomonas chloritidismutans sp. nov., a non-denitrifying, chlorate-reducing bacterium.	Wolterink AFWM, Jonker AB, Kengen SWM, Stams AJM	Int J Syst Evol Microbiol	10.1099/00207713-52-6-2183	2002

References

#5100	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13592
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#34277	; Curators of the CIP;
#59220	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 51509
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123337	Collection of Institut Pasteur ; Curators of the CIP; CIP 107870
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Stutzerimonas chloritidismutans (DSM 13592, KCTC 12116, CCUG 51509)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000495915

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

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