Rhodococcus opacus DSM 1069 is a mesophilic prokaryote that was isolated from field soil.
mesophilic| @ref 20215 |
Last LPSN update
2026-02-09 11:20:25 |
| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Nocardiaceae |
| Genus Rhodococcus |
| Species Rhodococcus opacus |
| Full scientific name Rhodococcus opacus Klatte et al. 1995 |
| Synonyms (2) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 415 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | Medium recipe at MediaDive  | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water | ||
| 415 | BHI MEDIUM (DSMZ Medium 215) | Medium recipe at MediaDive | Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water | ||
| 415 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | Medium recipe at MediaDive | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
| @ref | Growth | Type | Temperature (°C) | Range | |
|---|---|---|---|---|---|
| 415 | positive | growth | 28 | mesophilic |
| 20216 | ObservationDegrades lignin |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68369 | 17128 ChEBI | adipate | + | assimilation | from API 20NE |
| 68369 | 29016 ChEBI | arginine | - | hydrolysis | from API 20NE |
| 68379 | 17634 ChEBI | D-glucose | - | fermentation | from API Coryne |
| 68369 | 17634 ChEBI | D-glucose | + | assimilation | from API 20NE |
| 68369 | 17634 ChEBI | D-glucose | - | fermentation | from API 20NE |
| 68379 | 16899 ChEBI | D-mannitol | - | fermentation | from API Coryne |
| 68369 | 16899 ChEBI | D-mannitol | + | assimilation | from API 20NE |
| 68379 | 16988 ChEBI | D-ribose | - | fermentation | from API Coryne |
| 68379 | 65327 ChEBI | D-xylose | - | fermentation | from API Coryne |
| 68369 | 27689 ChEBI | decanoate | - | assimilation | from API 20NE |
| 68379 | 4853 ChEBI | esculin | - | hydrolysis | from API Coryne |
| 68369 | 4853 ChEBI | esculin | - | hydrolysis | from API 20NE |
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 68369 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20NE |
| 68369 | 24265 ChEBI | gluconate | + | assimilation | from API 20NE |
| 68379 | 28087 ChEBI | glycogen | - | fermentation | from API Coryne |
| 68379 | 17716 ChEBI | lactose | - | fermentation | from API Coryne |
| 68369 | 25115 ChEBI | malate | + | assimilation | from API 20NE |
| 68379 | 17306 ChEBI | maltose | - | fermentation | from API Coryne |
| 68369 | 17306 ChEBI | maltose | + | assimilation | from API 20NE |
| 68369 | 59640 ChEBI | N-acetylglucosamine | + | assimilation | from API 20NE |
| 68379 | 17632 ChEBI | nitrate | - | reduction | from API Coryne |
| 68369 | 17632 ChEBI | nitrate | - | reduction | from API 20NE |
| 68379 | 17992 ChEBI | sucrose | - | fermentation | from API Coryne |
| 68369 | 27897 ChEBI | tryptophan | - | energy source | from API 20NE |
| 68379 | 16199 ChEBI | urea | - | hydrolysis | from API Coryne |
| 68369 | 16199 ChEBI | urea | - | hydrolysis | from API 20NE |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68379 | alpha-glucosidase | + | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 68369 | arginine dihydrolase | - | 3.5.3.6 | from API 20NE |
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 68379 | beta-galactosidase | - | 3.2.1.23 | from API Coryne |
| 68379 | beta-glucosidase | - | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucosidase | - | 3.2.1.21 | from API zym |
| 68369 | beta-glucosidase | - | 3.2.1.21 | from API 20NE |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 68382 | cystine arylamidase | - | 3.4.11.3 | from API zym |
| 68382 | esterase (C 4) | - | from API zym | |
| 68379 | gelatinase | - | from API Coryne | |
| 68369 | gelatinase | - | from API 20NE | |
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 68382 | lipase (C 14) | - | from API zym | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 68379 | pyrazinamidase | - | 3.5.1.B15 | from API Coryne |
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 68379 | urease | - | 3.5.1.5 | from API Coryne |
| 68369 | urease | - | 3.5.1.5 | from API 20NE |
| @ref | Control | Alkaline phosphatase | Esterase (C 4) | 2-naphtyl caprylateEsterase Lipase (C 8) | Lipase (C 14) | L-leucyl-2-naphthylamideLeucine arylamidase | L-valyl-2-naphthylamideValine arylamidase | L-cystyl-2-naphthylamideCystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 415 | - | - | - | + | - | + | +/- | - | - | - | + | +/- | - | - | - | + | - | - | - | - | |
| 415 | - | + | - | +/- | - | + | +/- | - | - | - | +/- | - | - | - | - | +/- | - | - | - | - |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Recent Biotechnology Advances in Bio-Conversion of Lignin to Lipids by Bacterial Cultures. | Wang H, Peng X, Li H, Giannis A, He C. | Front Chem | 10.3389/fchem.2022.894593 | 2022 | | |
| Engineering of Rhodococcus jostii RHA1 for utilisation of carboxymethylcellulose. | Yasin R, Rashid GMM, Ali I, Bugg TDH. | Heliyon | 10.1016/j.heliyon.2023.e19511 | 2023 | | |
| Lignin-Degrading Enzymes and the Potential of Pseudomonas putida as a Cell Factory for Lignin Degradation and Valorization. | Zhou Q, Fransen A, de Winde H. | Microorganisms | 10.3390/microorganisms13040935 | 2025 | | |
| Microbial Lipid Based Biorefinery Concepts: A Review of Status and Prospects. | Silva JME, Martins LHDS, Moreira DKT, Silva LDP, Barbosa PPM, Komesu A, Ferreira NR, Oliveira JAR. | Foods | 10.3390/foods12102074 | 2023 | | |
| Innovations and developments in single cell protein: Bibliometric review and patents analysis. | Ribeiro GO, Rodrigues LAP, Dos Santos TBS, Alves JPS, Oliveira RS, Nery TBR, Barbosa JDV, Soares MBP. | Front Microbiol | 10.3389/fmicb.2022.1093464 | 2022 | | |
| Recent Advancements and Challenges in Lignin Valorization: Green Routes towards Sustainable Bioproducts. | Ullah M, Liu P, Xie S, Sun S. | Molecules | 10.3390/molecules27186055 | 2022 | | |
| Bacterial transformation of lignin: key enzymes and high-value products. | Gu J, Qiu Q, Yu Y, Sun X, Tian K, Chang M, Wang Y, Zhang F, Huo H. | Biotechnol Biofuels Bioprod | 10.1186/s13068-023-02447-4 | 2024 | | |
| Bacterial Transformation of Aromatic Monomers in Softwood Black Liquor. | Navas LE, Dexter G, Liu J, Levy-Booth D, Cho M, Jang SK, Mansfield SD, Renneckar S, Mohn WW, Eltis LD. | Front Microbiol | 10.3389/fmicb.2021.735000 | 2021 | | |
| Combinatorial pretreatment and fermentation optimization enabled a record yield on lignin bioconversion. | Liu ZH, Xie S, Lin F, Jin M, Yuan JS. | Biotechnol Biofuels | 10.1186/s13068-018-1021-3 | 2018 | | |
| Metabolism | Rhodococcus as Biofactories for Microbial Oil Production. | Alvarez HM, Hernandez MA, Lanfranconi MP, Silva RA, Villalba MS. | Molecules | 10.3390/molecules26164871 | 2021 | |
| Bacterial Valorization of Lignin: Strains, Enzymes, Conversion Pathways, Biosensors, and Perspectives. | Lee S, Kang M, Bae JH, Sohn JH, Sung BH. | Front Bioeng Biotechnol | 10.3389/fbioe.2019.00209 | 2019 | | |
| Metabolism | Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products. | Xu Z, Lei P, Zhai R, Wen Z, Jin M. | Biotechnol Biofuels | 10.1186/s13068-019-1376-0 | 2019 | |
| Depolymerization and conversion of lignin to value-added bioproducts by microbial and enzymatic catalysis. | Weng C, Peng X, Han Y. | Biotechnol Biofuels | 10.1186/s13068-021-01934-w | 2021 | | |
| Metabolism | Cultivation of lipid-producing bacteria with lignocellulosic biomass: effects of inhibitory compounds of lignocellulosic hydrolysates. | Wang B, Rezenom YH, Cho KC, Tran JL, Lee DG, Russell DH, Gill JJ, Young R, Chu KH. | Bioresour Technol | 10.1016/j.biortech.2014.02.133 | 2014 | |
| Metabolism | A Review on the Utilization of Lignin as a Fermentation Substrate to Produce Lignin-Modifying Enzymes and Other Value-Added Products. | Iram A, Berenjian A, Demirci A. | Molecules | 10.3390/molecules26102960 | 2021 | |
| Endophytes in Lignin Valorization: A Novel Approach. | Mattoo AJ, Nonzom S. | Front Bioeng Biotechnol | 10.3389/fbioe.2022.895414 | 2022 | | |
| Metabolism | Metabolism of aromatics by Trichosporon oleaginosus while remaining oleaginous. | Yaguchi A, Robinson A, Mihealsick E, Blenner M. | Microb Cell Fact | 10.1186/s12934-017-0820-8 | 2017 | |
| Metabolism | Microbial bioprospecting for lignocellulose degradation at a unique Greek environment. | Georgiadou DN, Avramidis P, Ioannou E, Hatzinikolaou DG. | Heliyon | 10.1016/j.heliyon.2021.e07122 | 2021 | |
| Biotechnology | New biofuel alternatives: integrating waste management and single cell oil production. | Martinez EJ, Raghavan V, Gonzalez-Andres F, Gomez X. | Int J Mol Sci | 10.3390/ijms16059385 | 2015 | |
| Metabolism | Gene probing reveals the widespread distribution, diversity and abundance of isoprene-degrading bacteria in the environment. | Carrion O, Larke-Mejia NL, Gibson L, Farhan Ul Haque M, Ramiro-Garcia J, McGenity TJ, Murrell JC. | Microbiome | 10.1186/s40168-018-0607-0 | 2018 | |
| Pathogenicity | Relationships between colony morphotypes and oil tolerance in Rhodococcus rhodochrous. | Iwabuchi N, Sunairi M, Anzai H, Nakajima M, Harayama S. | Appl Environ Microbiol | 10.1128/aem.66.11.5073-5077.2000 | 2000 | |
| An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries. | Patel A, Karageorgou D, Rova E, Katapodis P, Rova U, Christakopoulos P, Matsakas L. | Microorganisms | 10.3390/microorganisms8030434 | 2020 | | |
| Metabolism | Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost. | Ravi K, Garcia-Hidalgo J, Gorwa-Grauslund MF, Liden G. | Appl Microbiol Biotechnol | 10.1007/s00253-017-8211-y | 2017 | |
| Synthesis and biological characterization of arylomycin B antibiotics. | Roberts TC, Smith PA, Romesberg FE. | J Nat Prod | 10.1021/np200163g | 2011 | | |
| Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains. | Li X, He Y, Zhang L, Xu Z, Ben H, Gaffrey MJ, Yang Y, Yang S, Yuan JS, Qian WJ, Yang B. | Biotechnol Biofuels | 10.1186/s13068-019-1395-x | 2019 | | |
| Transposition of the IS21-related element IS1415 in Rhodococcus erythropolis. | Nagy I, Schoofs G, Vanderleyden J, De Mot R. | J Bacteriol | 10.1128/jb.179.14.4635-4638.1997 | 1997 | | |
| Bacterial conversion of depolymerized Kraft lignin. | Ravi K, Abdelaziz OY, Nobel M, Garcia-Hidalgo J, Gorwa-Grauslund MF, Hulteberg CP, Liden G | Biotechnol Biofuels | 10.1186/s13068-019-1397-8 | 2019 | | |
| Metabolism | Production of single cell protein from agro-waste using Rhodococcus opacus. | Mahan KM, Le RK, Wells T Jr, Anderson S, Yuan JS, Stoklosa RJ, Bhalla A, Hodge DB, Ragauskas AJ | J Ind Microbiol Biotechnol | 10.1007/s10295-018-2043-3 | 2018 | |
| Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus. | Le RK, Das P, Mahan KM, Anderson SA, Wells T Jr, Yuan JS, Ragauskas AJ | AMB Express | 10.1186/s13568-017-0484-0 | 2017 | | |
| Metabolism | Microbial lipid production by oleaginous Rhodococci cultured in lignocellulosic autohydrolysates. | Wei Z, Zeng G, Huang F, Kosa M, Sun Q, Meng X, Huang D, Ragauskas AJ | Appl Microbiol Biotechnol | 10.1007/s00253-015-6752-5 | 2015 | |
| Metabolism | Pyrolysis oil-based lipid production as biodiesel feedstock by Rhodococcus opacus. | Wei Z, Zeng G, Kosa M, Huang D, Ragauskas AJ | Appl Biochem Biotechnol | 10.1007/s12010-014-1305-4 | 2014 | |
| Metabolism | Bioconversion of lignin model compounds with oleaginous Rhodococci. | Kosa M, Ragauskas AJ | Appl Microbiol Biotechnol | 10.1007/s00253-011-3743-z | 2011 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive10996.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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