Rhodococcus opacus (DSM 43250, ATCC 51882)

Cell morphology

Multimedia

• @ref:	10906
  multimedia content:	DSM_43250.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43250.jpg
  caption:	Medium 83 28°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
10906	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)		Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
10906	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

Culture temp

Oxygen tolerance

Isolation source categories

Isolation

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score	IMG accession
66792	Rhodococcus opacus ATCC 51882	complete			37919	94.95	2681812806
66792	ASM436507v1 assembly for Rhodococcus opacus	contig	GCA_004365075	37919.36	37919	2.96

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	One-step biosynthesis of alpha-ketoisocaproate from L-leucine by an Escherichia coli whole-cell biocatalyst expressing an L-amino acid deaminase from Proteus vulgaris.	Song Y, Li J, Shin HD, Du G, Liu L, Chen J.	Sci Rep	10.1038/srep12614	2015
Enzymology	Crystallization and preliminary X-ray analysis of a bacterial L-amino-acid oxidase from Rhodococcus opacus.	Faust A, Geueke B, Niefind K, Hummel W, Schomburg D.	Acta Crystallogr Sect F Struct Biol Cryst Commun	10.1107/s1744309106005689	2006
Metabolism	Tuning the transcription and translation of L-amino acid deaminase in Escherichia coli improves alpha-ketoisocaproate production from L-leucine.	Song Y, Li J, Shin HD, Liu L, Du G, Chen J.	PLoS One	10.1371/journal.pone.0179229	2017
Metabolism	SO-LAAO, a novel L-amino acid oxidase that enables Streptococcus oligofermentans to outcompete Streptococcus mutans by generating H2O2 from peptone.	Tong H, Chen W, Shi W, Qi F, Dong X.	J Bacteriol	10.1128/jb.00363-08	2008
Genetics	Metagenomic analysis of soybean endosphere microbiome to reveal signatures of microbes for health and disease.	Chouhan U, Gamad U, Choudhari JK.	J Genet Eng Biotechnol	10.1186/s43141-023-00535-4	2023
Pathogenicity	Relationships between colony morphotypes and oil tolerance in Rhodococcus rhodochrous.	Iwabuchi N, Sunairi M, Anzai H, Nakajima M, Harayama S.	Appl Environ Microbiol	10.1128/aem.66.11.5073-5077.2000	2000
Phylogeny	Rapid identification of Nocardia farcinica clinical isolates by a PCR assay targeting a 314-base-pair species-specific DNA fragment.	Brown JM, Pham KN, McNeil MM, Lasker BA.	J Clin Microbiol	10.1128/jcm.42.8.3655-3660.2004	2004
Metabolism	Carbon and hydrogen isotopic fractionation during abiotic hydrolysis and aerobic biodegradation of phthalate esters.	Zhang D, Wu L, Yao J, Vogt C, Richnow HH	Sci Total Environ	10.1016/j.scitotenv.2019.01.003	2019
Metabolism	Production of alpha-ketoisocaproate via free-whole-cell biotransformation by Rhodococcus opacus DSM 43250 with L-leucine as the substrate.	Zhu Y, Li J, Liu L, Du G, Chen J	Enzyme Microb Technol	10.1016/j.enzmictec.2011.06.008	2011