Name: Rhodococcus opacus R7
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 136650

Type strain:

Species: Rhodococcus opacus

Strain Designation: R7

Culture col. no.: CIP 107348

Strain history: CIP <- 2002, G. Bestetti, Milano Univ., Milano, Italy: strain R7

NCBI tax ID(s): 37919 (species)

SI-ID 362149

CIP 107348

Other strains from Rhodococcus opacus

R. opacus 1b, 18, DSM 43205, ATCC 51881, ... (type strain)
R. opacus DSM 1069
R. opacus C125, DSM 8531
R. opacus DSM 43206, ATCC 21499, DSM 441, ...
R. opacus DSM 43250, ATCC 51882
R. opacus DSM 43251
R. opacus Pellegrino 327, DSM 43252, CIP ...
R. opacus S676, 225, DSM 43280, ATCC 4275, ...
R. opacus 691-19, DSM 43943, IMET 7363
R. opacus DSM 44072, CBS 330.61
R. opacus DSM 44236
R. opacus MoAcy1, DSM 44186
R. opacus Tue Acy 1, DSM 44188
R. opacus 305-97, L2aT3, PWD5, DSM 44304
R. opacus 307-97, PWD7, S2aT5, S₂aT5, ...
R. opacus 310-97, L1aT1, PWD10, DSM 44307
R. opacus GW-86a, DSM 44311
R. opacus Bie-17, DSM 44312
R. opacus 304-97, H1aT2, PWD4, DSM 44313
R. opacus 315-97, PWD15, S1aT1, DSM 44314
R. opacus 97-319, H3aT3, PWD19, DSM 44315
R. opacus ISO-5, DSM 44600
R. opacus ROL1, DSM 44853
R. opacus ROL2, DSM 44854
R. opacus R1L6, DSM 44855
R. opacus DSM 46027, IMET 7365, NCIB 9702
R. opacus ST033313(HKI),
R. opacus 1CP, DSM 46757, VKM Ac-2638
R. opacus GK 3, GK3, CIP 110889, CCMM B1295

Section

Rhodococcus opacus R7 is an obligate aerobe, mesophilic, Gram-positive prokaryote.

Gram-positive
rod-shaped
mesophilic
obligate aerobe
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Genus	*Rhodococcus*
	Species	**Rhodococcus opacus**
	Full Scientific Name (LPSN)	Rhodococcus opacus Klatte et al. 1995

	Synonym	Rhodococcus imtechensis
	Synonym	Rhodococcus percolatus

Information on morphological and physiological properties Morphology

[Ref.: #36128]	Gram stain	positive

[Ref.: #36128]	Cell shape	rod-shaped

[Ref.: #36128]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #36128]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #36128]

Culture medium growth

[Ref.: #36128]

Culture medium composition

expand / minimize

[Ref.: #36128]

Culture medium

CIP Medium 72

[Ref.: #36128]

Culture medium growth

[Ref.: #36128]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #36128]		growth	30 °C
[Ref.: #36128]		growth	10-30 °C
[Ref.: #36128]	no	growth	37 °C
[Ref.: #36128]	no	growth	41 °C
[Ref.: #36128]	no	growth	45 °C

[Ref.: #36128]	Temperature range	mesophilic
[Ref.: #36128]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #36128]

Oxygen tolerance

obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #36128]	NaCl		growth	0-6	%
[Ref.: #36128]	NaCl	no	growth	8	%
[Ref.: #36128]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #36128]	citrate ChEBI	-	carbon source
[Ref.: #36128]	esculin ChEBI	-	hydrolysis
[Ref.: #36128]	hippurate ChEBI	+	hydrolysis
[Ref.: #36128]	nitrate ChEBI	-	reduction
[Ref.: #36128]	nitrate ChEBI	-	respiration
[Ref.: #36128]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #36128]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #36128]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #36128]		acetoin ChEBI	-
[Ref.: #36128]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #36128]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #36128]	amylase	-
[Ref.: #36128]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #36128]	caseinase	-	3.4.21.50
[Ref.: #36128]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #36128]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #36128]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #36128]	gelatinase	-
[Ref.: #36128]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #36128]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #36128]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #36128]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #36128]	oxidase	-
[Ref.: #36128]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #36128]	tryptophan deaminase	-
[Ref.: #36128]	tween esterase	+
[Ref.: #36128]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #36128]	2168	not determinedn.d.	+	+	-	+	-	-	-	+	-	+	+	-	-	-	-	-	-	-	+	+	-	-	+	+	-	+	+	-	-	-	-	-	+	+	+	+	+	-	-	-	-	-	-	+	-	+	-	+	-	+	+	+	-	+	-	-	+	+	-	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	+	-	-	-	+	+	+	-	+	+	-	+	+	+	+	-	+	+	-	-	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #36128]	10285	-	-	+	+	-	+	+	+	-	-	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #36128]	Sample type/isolated from	Environment, Contaminated soil
[Ref.: #36128]	Geographic location (country and/or sea, region)	Crespiatica Lodi
[Ref.: #36128]	Country	Italy
[Ref.: #36128]	Country ISO 3 Code	ITA
[Ref.: #36128]	Continent	Europe
[Ref.: #36128]	Isolation date	2000

[Ref.: #36128]	Geographic location (country and/or sea, region)	Crespiatica Lodi
[Ref.: #36128]	Country	Italy
[Ref.: #36128]	Country ISO 3 Code	ITA
[Ref.: #36128]	Continent	Europe
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #36128]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Rhodococcus opacus strain R7	37919.10	complete	PATRIC	37919 tax ID	*
[Ref.: #66792]	Rhodococcus opacus strain R7	37919.22	plasmid	PATRIC	37919 tax ID	*
[Ref.: #66792]	Rhodococcus opacus strain R7	37919.23	plasmid	PATRIC	37919 tax ID	*
[Ref.: #66792]	Rhodococcus opacus strain R7	37919.21	plasmid	PATRIC	37919 tax ID	*
[Ref.: #66792]	Rhodococcus opacus strain R7	37919.24	plasmid	PATRIC	37919 tax ID	*
[Ref.: #66792]	Rhodococcus opacus strain R7	37919.20	plasmid	PATRIC	37919 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Rhodococcus opacus strain R7 PATRIC: 37919.10
[Ref.: #69480]	gram-positive	yes	81.12	no
[Ref.: #69480]	anaerobic	no	98.837	no
[Ref.: #69480]	halophile	no	90.495	no
[Ref.: #69480]	spore-forming	no	57.846	no
[Ref.: #69480]	glucose-util	yes	92.234	no
[Ref.: #69480]	aerobic	yes	86.403	no
[Ref.: #69480]	motile	no	92.448	no
[Ref.: #69480]	flagellated	no	98.072	no
[Ref.: #69480]	thermophile	no	99.624	yes
[Ref.: #69480]	glucose-ferment	no	89.867	no

Availability in culture collections External links

[Ref.: #36128]

Culture collection no.

CIP 107348

[Ref.: #93755]

SI-ID 362149

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome Sequence of Rhodococcus opacus Strain R7, a Biodegrader of Mono- and Polycyclic Aromatic Hydrocarbons.	Di Gennaro P, Zampolli J, Presti I, Cappelletti M, D'Ursi P, Orro A, Mezzelani A, Milanesi L	Genome Announc	10.1128/genomeA.00827-14	2014	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#36128 Collection of Institut Pasteur ; Curators of the CIP; CIP 107348

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#93755

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID362149.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Rhodococcus opacus in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Rhodococcus opacus strain R7

Change proposal

Change proposal