Rhodococcus opacus 1b, 18 | Type strain | DSM 43205, ATCC 51881, CIP 104549, DSM 427, JCM 9703, CGMCC 4.1820, IFO 16217, KCTC 9811, LMG 18000, NBRC 100624, NBRC 16217, NCIMB 10810, NCIMB 13353

Caption:

Medium 535 28°C

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

[Ref.: #35692]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #35692]

Culture medium growth

[Ref.: #35692]

Culture medium composition

[Ref.: #41630]

Culture medium

MEDIUM 29- Brain heart agar

[Ref.: #41630]

Culture medium growth

[Ref.: #41630]

Culture medium composition

Culture medium

CIP Medium 3

Culture medium growth

Medium recipe provided by DSMZ

Culture medium link

	Kind of temperature		Temperature
[Ref.: #10865]		growth	28 °C
[Ref.: #35692]		growth	30 °C
[Ref.: #41630]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #121422]		growth	10-37 °C
[Ref.: #121422]	no	growth	41 °C
[Ref.: #121422]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

What are isolation sources categories?

Oxygen tolerance

obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #18483]	NaCl		maximum	7.5	%
[Ref.: #121422]	NaCl		growth	0-6	%
[Ref.: #121422]	NaCl	no	growth	8	%
[Ref.: #121422]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: MK-8(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #121422]	esculin ChEBI	-	hydrolysis
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E, API Coryne
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #121422]	hippurate ChEBI	-	hydrolysis
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #121422]	nitrate ChEBI	+	reduction
[Ref.: #121422]	nitrate ChEBI	-	respiration
[Ref.: #121422]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #121422]	acetoin ChEBI	-
[Ref.: #68368]	acetoin ChEBI	-		* from API 20E
[Ref.: #68368]	indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #121422]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121422]	amylase	+
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #121422]	beta-galactosidase	+	3.2.1.23
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne, API 20E, API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #121422]	catalase	+	1.11.1.6
[Ref.: #121422]	DNase	-
[Ref.: #121422]	gelatinase	-
[Ref.: #68379]	gelatinase	-		* from API Coryne, API 20E
[Ref.: #121422]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121422]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #121422]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #121422]	oxidase	-
[Ref.: #121422]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #121422]	tween esterase	-
[Ref.: #68368]	urease	+	3.5.1.5	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #18483]	67	+	+	-	+	+	-	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121422]	708	not determinedn.d.	+	+	+	-	-	-	+	+	+	+	-	+	+	+	+	-	-	-	+	-	-	-	+	-	-	+	+	-	-	-	-	-	+	+	+	+	+	-	-	-	-	+	-	+	-	+	+	-	-	+	+	+	-	+	-	-	+	+	-	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	+	-	-	-	+	+	+	-	-	+	-	-	-	+	+	-	-	+	+	-	+	+	+	+

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18483]	136	-	-	-	-	-	+	+	-	-	+	-	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.
[Ref.: #10865]	4240	+	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18483]	183	not determinedn.d.	-	-	-	-	+	+	+	+	-	-	+	+	+	-	+	+	-	-	-
[Ref.: #10865]	14134	-	+	-	-	-	+	+/-	-	-	-	+/-	-	-	+	-	+/-	-	-	-	-
[Ref.: #121422]	8033	-	-	+	+	-	+	+	-	-	-	+	-	+	+	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #10865]	Sample type/isolated from	soil

[Ref.: #67770]	Sample type/isolated from	Soil from the surroundings of a defective town gas pipe
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18483]	Biosafety level	1	German classification
[Ref.: #10865]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121422]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Rhodococcus opacus partial 16S rRNA gene, strain DSM 43205, isolate Clone 1	LN827918	1510	GenBank ENA	37919 tax ID
[Ref.: #67770]	Rhodococcus opacus partial 16S rRNA gene, strain DSM 43205, isolate Clone 2	LN827919	1510	GenBank ENA	37919 tax ID
[Ref.: #67770]	Rhodococcus opacus partial 16S rRNA gene, strain DSM 43205, isolate Clone 3	LN827920	1509	GenBank ENA	37919 tax ID
[Ref.: #20218]	Rhodococcus opacus 16S rRNA gene, strain DSM43205T	X80630	1481	GenBank ENA	37919 tax ID	*
[Ref.: #124043]	Rhodococcus opacus partial 16S rRNA gene, strain DSM 43205, isolate Clone 4	LN827921	1510	GenBank ENA	37919 tax ID	*
[Ref.: #124043]	Rhodococcus opacus partial 16S rRNA gene, strain DSM 43205, isolate Clone 5	LN827922	1509	GenBank ENA	37919 tax ID	*
[Ref.: #124043]	Rhodococcus opacus partial 16S rRNA gene, strain DSM 43205, isolate Clone 6	LN827923	1510	GenBank ENA	37919 tax ID	*
[Ref.: #124043]	Rhodococcus opacus partial 16S rRNA gene, strain DSM 43205, isolate Clone 7	LN827924	1510	GenBank ENA	37919 tax ID	*
[Ref.: #20218]	Rhodococcus opacus strain DSM 43205 note copy 1 16S-23S ribosomal RNA intergenic spacer, complete sequence	AF536493	301	GenBank ENA	37919 tax ID	*
[Ref.: #20218]	Rhodococcus opacus strain DSM 43205 note copy 2 16S-23S ribosomal RNA intergenic spacer, complete sequence	AF536494	308	GenBank ENA	37919 tax ID	*
[Ref.: #20218]	Rhodococcus opacus strain DSM 43205 note copy 3 16S-23S ribosomal RNA intergenic spacer, complete sequence	AF536495	302	GenBank ENA	37919 tax ID	*
[Ref.: #20218]	Rhodococcus opacus strain DSM 43205 16S ribosomal RNA gene, partial sequence	KF410349	1481	GenBank ENA	37919 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Rhodococcus opacus ATCC 51881	GCA_012396235	contig	GenBank ENA	37919 tax ID
[Ref.: #67770]	Rhodococcus opacus DSM 43205	GCA_001646735	contig	GenBank ENA	37919 tax ID
[Ref.: #66792]	Rhodococcus opacus strain ATCC 51881	37919.38	wgs	PATRIC	37919 tax ID	*
[Ref.: #66792]	Rhodococcus opacus strain DSM 43205	37919.12	wgs	PATRIC	37919 tax ID	*
[Ref.: #66792]	Rhodococcus opacus DSM 43205	2744054613	draft	JGI IMG/M	37919 tax ID	*
[Ref.: #66792]	Rhodococcus opacus DSM 43205	GCA_910591545	contig	GenBank ENA	37919 tax ID	*

[Ref.: #67770]

GC-content

67.6 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Rhodococcus opacus strain DSM 43205 PATRIC: 37919.12
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	83	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Rhodococcus opacus strain ATCC 51881 PATRIC: 37919.38
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	85.999	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	97.589	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	62.117	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	89.933	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	98	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	91.5	no

Availability in culture collections External links