Rhodococcus opacus 1b is an obligate aerobe, mesophilic, Gram-positive prokaryote that was isolated from soil.
Gram-positive rod-shaped obligate aerobe mesophilic genome sequence 16S sequence| @ref 20215 |
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| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Nocardiaceae |
| Genus Rhodococcus |
| Species Rhodococcus opacus |
| Full scientific name Rhodococcus opacus Klatte et al. 1995 |
| Synonyms (2) |
| @ref: | 10865 |
| multimedia content: | DSM_43205.jpg |
| multimedia.multimedia content: | https://www.dsmz.de/microorganisms/photos/DSM_43205.jpg |
| caption: | Medium 535 28°C |
| intellectual property rights: | © Leibniz-Institut DSMZ |
| manual_annotation: | 1 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 10865 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | Medium recipe at MediaDive | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water | ||
| 10865 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | Medium recipe at MediaDive | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water | ||
| 35692 | MEDIUM 3 - Columbia agar | Columbia agar (39.000 g);distilled water (1000.000 ml) | |||
| 41630 | MEDIUM 29- Brain heart agar | Distilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g) | |||
| 121422 | CIP Medium 3 | Medium recipe at CIP |
| 67770 | Observationquinones: MK-8(H2) |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68369 | 17128 ChEBI | adipate | + | assimilation | from API 20NE |
| 68369 | 29016 ChEBI | arginine | - | hydrolysis | from API 20NE |
| 68368 | 29016 ChEBI | arginine | + | hydrolysis | from API 20E |
| 68368 | 16947 ChEBI | citrate | + | assimilation | from API 20E |
| 68379 | 17634 ChEBI | D-glucose | - | fermentation | from API Coryne |
| 68369 | 17634 ChEBI | D-glucose | + | assimilation | from API 20NE |
| 68369 | 17634 ChEBI | D-glucose | - | fermentation | from API 20NE |
| 68379 | 16899 ChEBI | D-mannitol | - | fermentation | from API Coryne |
| 68369 | 16899 ChEBI | D-mannitol | + | assimilation | from API 20NE |
| 68379 | 16988 ChEBI | D-ribose | - | fermentation | from API Coryne |
| 68379 | 65327 ChEBI | D-xylose | - | fermentation | from API Coryne |
| 68369 | 27689 ChEBI | decanoate | - | assimilation | from API 20NE |
| 68379 | 4853 ChEBI | esculin | - | hydrolysis | from API Coryne |
| 121422 | 4853 ChEBI | esculin | - | hydrolysis | |
| 68369 | 4853 ChEBI | esculin | - | hydrolysis | from API 20NE |
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 68369 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20NE |
| 68368 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20E |
| 68369 | 24265 ChEBI | gluconate | + | assimilation | from API 20NE |
| 68379 | 28087 ChEBI | glycogen | - | fermentation | from API Coryne |
| 121422 | 606565 ChEBI | hippurate | - | hydrolysis | |
| 68379 | 17716 ChEBI | lactose | - | fermentation | from API Coryne |
| 68368 | 25094 ChEBI | lysine | - | degradation | from API 20E |
| 68369 | 25115 ChEBI | malate | + | assimilation | from API 20NE |
| 68379 | 17306 ChEBI | maltose | - | fermentation | from API Coryne |
| 68369 | 59640 ChEBI | N-acetylglucosamine | + | assimilation | from API 20NE |
| 121422 | 17632 ChEBI | nitrate | + | reduction | |
| 121422 | 17632 ChEBI | nitrate | - | respiration | |
| 68369 | 17632 ChEBI | nitrate | + | reduction | from API 20NE |
| 121422 | 16301 ChEBI | nitrite | - | reduction | |
| 68368 | 18257 ChEBI | ornithine | + | degradation | from API 20E |
| 68369 | 27897 ChEBI | tryptophan | - | energy source | from API 20NE |
| 68368 | 27897 ChEBI | tryptophan | - | energy source | from API 20E |
| 68369 | 16199 ChEBI | urea | - | hydrolysis | from API 20NE |
| 68368 | 16199 ChEBI | urea | + | hydrolysis | from API 20E |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 121422 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 121422 | amylase | + | ||
| 68369 | arginine dihydrolase | - | 3.5.3.6 | from API 20NE |
| 68368 | arginine dihydrolase | + | 3.5.3.6 | from API 20E |
| 68382 | beta-galactosidase | + | 3.2.1.23 | from API zym |
| 121422 | beta-galactosidase | + | 3.2.1.23 | |
| 68379 | beta-galactosidase | + | 3.2.1.23 | from API Coryne |
| 68368 | beta-galactosidase | + | 3.2.1.23 | from API 20E |
| 68369 | beta-glucosidase | - | 3.2.1.21 | from API 20NE |
| 68379 | beta-glucosidase | - | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 121422 | catalase | + | 1.11.1.6 | |
| 121422 | DNase | - | ||
| 121422 | gelatinase | - | ||
| 68379 | gelatinase | - | from API Coryne | |
| 68369 | gelatinase | - | from API 20NE | |
| 68368 | gelatinase | - | from API 20E | |
| 121422 | lecithinase | - | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 68382 | lipase (C 14) | - | from API zym | |
| 121422 | lysine decarboxylase | - | 4.1.1.18 | |
| 68368 | lysine decarboxylase | - | 4.1.1.18 | from API 20E |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 121422 | ornithine decarboxylase | - | 4.1.1.17 | |
| 68368 | ornithine decarboxylase | + | 4.1.1.17 | from API 20E |
| 121422 | oxidase | - | ||
| 121422 | phenylalanine ammonia-lyase | - | 4.3.1.24 | |
| 68379 | pyrazinamidase | - | 3.5.1.B15 | from API Coryne |
| 68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 | from API Coryne |
| 68368 | tryptophan deaminase | - | 4.1.99.1 | from API 20E |
| 121422 | tween esterase | - | ||
| 68369 | urease | - | 3.5.1.5 | from API 20NE |
| 68368 | urease | + | 3.5.1.5 | from API 20E |
| @ref | Reduction of nitrateNIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | Control fermentationControl | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG | CAT | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 18483 | - | - | - | - | - | + | + | - | - | + | - | not determinedn.d. | - | - | - | - | - | - | + | - | not determinedn.d. | |
| 10865 | + | - | - | + | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | not determinedn.d. |
| @ref | Control | Alkaline phosphatase | Esterase (C 4) | 2-naphtyl caprylateEsterase Lipase (C 8) | Lipase (C 14) | L-leucyl-2-naphthylamideLeucine arylamidase | L-valyl-2-naphthylamideValine arylamidase | L-cystyl-2-naphthylamideCystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 18483 | not determinedn.d. | - | - | - | - | + | + | + | + | - | - | + | + | + | - | + | + | - | - | - | |
| 10865 | - | + | - | - | - | + | +/- | - | - | - | +/- | - | - | + | - | +/- | - | - | - | - | |
| 121422 | - | - | + | + | - | + | + | - | - | - | + | - | + | + | - | + | - | - | - | - | |
| 10865 | - | + | - | - | - | + | + | +/- | - | - | + | - | + | + | - | + | - | - | - | - |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 124043 | ASM4828834v1 assembly for Rhodococcus opacus DSM 43205 | complete | 37919 | 95.11 | ||||
| 66792 | ASM91059154v1 assembly for Rhodococcus opacus DSM 43205 | contig | 37919 | 69.8 | ||||
| 67770 | ASM1239623v1 assembly for Rhodococcus opacus ATCC 51881 | contig | 37919 | 56.66 | ||||
| 67770 | ASM164673v1 assembly for Rhodococcus opacus DSM 43205 | contig | 37919 | 1.08 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Rhodococcus opacus strain DSM 43205 note copy 1 16S-23S ribosomal RNA intergenic spacer, complete sequence | AF536493 | 301 | 37919 | ||
| 20218 | Rhodococcus opacus strain DSM 43205 note copy 2 16S-23S ribosomal RNA intergenic spacer, complete sequence | AF536494 | 308 | 37919 | ||
| 20218 | Rhodococcus opacus strain DSM 43205 note copy 3 16S-23S ribosomal RNA intergenic spacer, complete sequence | AF536495 | 302 | 37919 | ||
| 20218 | Rhodococcus opacus strain DSM 43205 16S ribosomal RNA gene, partial sequence | KF410349 | 1481 | 37919 | ||
| 20218 | Rhodococcus opacus 16S rRNA gene, strain DSM43205T | X80630 | 1481 | 37919 | ||
| 67770 | Rhodococcus opacus partial 16S rRNA gene, strain DSM 43205, isolate Clone 1 | LN827918 | 1510 | 37919 | ||
| 67770 | Rhodococcus opacus partial 16S rRNA gene, strain DSM 43205, isolate Clone 2 | LN827919 | 1510 | 37919 | ||
| 67770 | Rhodococcus opacus partial 16S rRNA gene, strain DSM 43205, isolate Clone 3 | LN827920 | 1509 | 37919 |
| @ref | GC-content (mol%) | Method | |
|---|---|---|---|
| 67770 | 67.6 | high performance liquid chromatography (HPLC) |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | spore_formation | BacteriaNetⓘ | no | 50.30 | no |
| 125439 | motility | BacteriaNetⓘ | no | 87.40 | no |
| 125439 | gram_stain | BacteriaNetⓘ | positive | 98.30 | no |
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 95.60 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 86.00 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 97.59 | no |
| 125438 | spore-forming | spore-formingⓘ | yes | 62.12 | no |
| 125438 | aerobic | aerobicⓘ | yes | 89.93 | yes |
| 125438 | thermophilic | thermophileⓘ | no | 98.00 | yes |
| 125438 | flagellated | motile2+ⓘ | no | 91.50 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Exploring New Strategies for Optimizing the Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from Methane and VFAs in Synthetic Cocultures and Mixed Methanotrophic Consortia. | Amabile C, Abate T, Marcos E, Chianese S, Musmarra D, Munoz R. | ACS Sustain Chem Eng | 10.1021/acssuschemeng.3c08570 | 2024 | ||
| Metabolism | Characterisation of the Paenarthrobacter nicotinovorans ATCC 49919 genome and identification of several strains harbouring a highly syntenic nic-genes cluster. | El-Sabeh A, Mlesnita AM, Munteanu IT, Honceriu I, Kallabi F, Boiangiu RS, Mihasan M. | BMC Genomics | 10.1186/s12864-023-09644-3 | 2023 | |
| Continuous Valorization of Carbon Dioxide into the Fine Chemical Ectoine by Hydrogenovibrio marinus: A New Strategy for Pharmaceutical Production. | Huang-Lin E, Lebrero R, Cantera S. | Environ Sci Technol | 10.1021/acs.est.4c12259 | 2025 | ||
| Enrichment of halotolerant hydrogen-oxidizing bacteria and production of high-value-added chemical hydroxyectoine using a hybrid biological-inorganic system. | Feng X, Kazama D, He S, Nakayama H, Hayashi T, Tokunaga T, Sato K, Kobayashi H. | Front Microbiol | 10.3389/fmicb.2023.1254451 | 2023 | ||
| Simultaneous heavy metal removal and anthracene biodegradation by the oleaginous bacteria Rhodococcus opacus. | Goswami L, Arul Manikandan N, Pakshirajan K, Pugazhenthi G. | 3 Biotech | 10.1007/s13205-016-0597-1 | 2017 | ||
| Metabolism | Rhodococcus as Biofactories for Microbial Oil Production. | Alvarez HM, Hernandez MA, Lanfranconi MP, Silva RA, Villalba MS. | Molecules | 10.3390/molecules26164871 | 2021 | |
| Targeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs. | Cho JS, Yang D, Prabowo CPS, Ghiffary MR, Han T, Choi KR, Moon CW, Zhou H, Ryu JY, Kim HU, Lee SY. | Nat Commun | 10.1038/s41467-023-38119-y | 2023 | ||
| Phylogeny | Identification and characterization of inorganic-phosphate-solubilizing bacteria from agricultural fields with a rapid isolation method. | Zheng BX, Ibrahim M, Zhang DP, Bi QF, Li HZ, Zhou GW, Ding K, Penuelas J, Zhu YG, Yang XR. | AMB Express | 10.1186/s13568-018-0575-6 | 2018 | |
| Metabolism | Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products. | Xu Z, Lei P, Zhai R, Wen Z, Jin M. | Biotechnol Biofuels | 10.1186/s13068-019-1376-0 | 2019 | |
| An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries. | Patel A, Karageorgou D, Rova E, Katapodis P, Rova U, Christakopoulos P, Matsakas L. | Microorganisms | 10.3390/microorganisms8030434 | 2020 | ||
| Depolymerization and conversion of lignin to value-added bioproducts by microbial and enzymatic catalysis. | Weng C, Peng X, Han Y. | Biotechnol Biofuels | 10.1186/s13068-021-01934-w | 2021 | ||
| Metabolism | Desulfonation and degradation of the disulfodiphenylethercarboxylates from linear alkyldiphenyletherdisulfonate surfactants. | Schleheck D, Lechner M, Schonenberger R, Suter MJ, Cook AM. | Appl Environ Microbiol | 10.1128/aem.69.2.938-944.2003 | 2003 | |
| Pathogenicity | Relationships between colony morphotypes and oil tolerance in Rhodococcus rhodochrous. | Iwabuchi N, Sunairi M, Anzai H, Nakajima M, Harayama S. | Appl Environ Microbiol | 10.1128/aem.66.11.5073-5077.2000 | 2000 | |
| Metabolism | Microcosm enrichment of biphenyl-degrading microbial communities from soils and sediments. | Wagner-Dobler I, Bennasar A, Vancanneyt M, Strompl C, Brummer I, Eichner C, Grammel I, Moore ER. | Appl Environ Microbiol | 10.1128/aem.64.8.3014-3022.1998 | 1998 | |
| Biotechnology | Production of biopolymer precursors beta-alanine and L-lactic acid from CO(2) with metabolically versatile Rhodococcus opacus DSM 43205. | Salusjarvi L, Ojala L, Peddinti G, Lienemann M, Jouhten P, Pitkanen JP, Toivari M | Front Bioeng Biotechnol | 10.3389/fbioe.2022.989481 | 2022 | |
| Phylogeny | Rhodococcus imtechensis sp. nov., a nitrophenol-degrading actinomycete. | Ghosh A, Paul D, Prakash D, Mayilraj S, Jain RK | Int J Syst Evol Microbiol | 10.1099/ijs.0.63939-0 | 2006 |
| #10865 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43205 |
| #18483 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #35692 | ; Curators of the CIP; |
| #41630 | ; Curators of the CIP; |
| #66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) . |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68368 | Automatically annotated from API 20E . |
| #68369 | Automatically annotated from API 20NE . |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #121422 | Collection of Institut Pasteur ; Curators of the CIP; CIP 104549 |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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