Rhodococcus opacus (DSM 43252, CIP 104626, Pellegrino 327)

Name: Rhodococcus opacus (DSM 43252, CIP 104626, Pellegrino 327)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11002

Type strain

Strain Designation Pellegrino 327

Culture col. no. DSM 43252 CIP 104626 Pellegrino 327

NCBI tax ID(s) 37919

Special Collections DSMZ CIP

History DSMZ <- P.R. Wallnöfer ("Rhodococcus rubrus", Nocardia sp.) CIP <- 1995, DSM, Rhodococcus ruber <- I. Tarnok: strain 5101, Nocardia pellegrino <- I. Szabo: strain Pellegrino 327

Links

Rhodococcus opacus Pellegrino 327 is an obligate aerobe, Gram-positive, rod-shaped bacterium that was isolated from soil.

Gram-positive rod-shaped obligate aerobe Bacteria

Name and taxonomic classification

SI-ID 50222

CIP 104626,DSM 43252

@ref 20215

Last LPSN update 2026-05-29 08:27:27

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Nocardiaceae

Genus Rhodococcus

Species Rhodococcus opacus

Full scientific name Rhodococcus opacus Klatte et al. 1995

Synonyms (2)

Rhodococcus percolatus
Rhodococcus imtechensis

BacDive ID	Other strains from **Rhodococcus opacus** (28)	Type strain
10998	R. opacus 1b, 18, DSM 43205, ATCC 51881, CIP 104549, ... (type strain)
10997	R. opacus C125, DSM 8531
10999	R. opacus DSM 43206, ATCC 21499, DSM 441, CIP 104550, ...
11000	R. opacus DSM 43250, ATCC 51882
11001	R. opacus DSM 43251
11003	R. opacus S676, 225, DSM 43280, ATCC 4275, IMET 7025, ...
11004	R. opacus 691-19, DSM 43943, IMET 7363
11005	R. opacus DSM 44072, CBS 330.61
11006	R. opacus DSM 44236
11007	R. opacus MoAcy1, DSM 44186
11008	R. opacus Tue Acy 1, DSM 44188
11010	R. opacus 305-97, L2aT3, PWD5, DSM 44304
11011	R. opacus 307-97, PWD7, S2aT5, S₂aT5, DSM ...
11012	R. opacus 310-97, L1aT1, PWD10, DSM 44307
11013	R. opacus GW-86a, DSM 44311
11014	R. opacus Bie-17, DSM 44312
11015	R. opacus 304-97, H1aT2, PWD4, DSM 44313
11016	R. opacus 315-97, PWD15, S1aT1, DSM 44314
11017	R. opacus 97-319, H3aT3, PWD19, DSM 44315
11018	R. opacus ISO-5, DSM 44600
11019	R. opacus ROL1, DSM 44853
11020	R. opacus ROL2, DSM 44854
11021	R. opacus R1L6, DSM 44855
11022	R. opacus DSM 46027, IMET 7365, NCIB 9702
136650	R. opacus R7, CIP 107348
160415	R. opacus 1CP, DSM 46757, VKM Ac-2638
173346	R. opacus GK 3, GK3, CIP 110889, CCMM B1295
178948	R. opacus IMET 07029, STI07029

Morphology

Cell morphology

@ref	Gram stain	Cell shape
121424	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
10908	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
39066	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
121424	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
10908	positive	growth	28
39066	positive	growth	30
121424	positive	growth	10-37
121424	negative	growth	41
121424	negative	growth	45

Physiology and metabolism

Oxygen tolerance

121424

Oxygen toleranceobligate aerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
121424	NaCl	positive	growth	0-6 %
121424	NaCl		growth	8 %
121424	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
121424	16947 ChEBI	citrate	+	carbon source
121424	17632 ChEBI	nitrate	+	reduction
121424	17632 ChEBI	nitrate	-	respiration
121424	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
121424	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
121424	15688 ChEBI	acetoin	-
121424	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
121424	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
121424	amylase	+
68382	beta-galactosidase	+	3.2.1.23	from API zym
121424	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121424	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
121424	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
121424	gamma-glutamyltransferase	+	2.3.2.2
121424	gelatinase	-
121424	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
121424	lipase	-
68382	lipase (C 14)	+		from API zym
121424	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121424	ornithine decarboxylase	-	4.1.1.17
121424	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
121424	urease	+	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121424	-	-	+	+	+	+	+	+	-	-	+	+	+	+	-	+	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
121424	not determinedn.d.	+	+	+	-	-	-	+	+	+	+	+	+	+	+	+	-	-	-	-	-	-	-	+	-	-	+	+	-	-	-	-	-	+	+	+	+	+	-	-	-	-	+	-	+	-	+	+	-	-	+	+	+	-	+	-	-	+	+	-	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	+	-	-	-	+	+	+	-	-	+	-	-	+	+	+	-	-	+	+	-	+	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type
10908	soil
121424	Environment, Soil

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
10908	1	Risk group (German classification) According to TRBA 466
121424	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome-based predictions

Literature

References

#10908	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43252
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#39066	; Curators of the CIP;
#68382	Automatically annotated from API zym .
#121424	Collection of Institut Pasteur ; Curators of the CIP; CIP 104626
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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