Name: Rhodococcus opacus 1CP
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 160415

Type strain:

Species: Rhodococcus opacus

Strain Designation: 1CP

Culture col. no.: DSM 46757, VKM Ac-2638

Strain history: <- D. Tischler, TU Bergakademie Freiberg, Germany; 1CP <- S.N. Gorlatov

NCBI tax ID(s): 37919 (species)

SI-ID 396785

DSM 46757

Other strains from Rhodococcus opacus

R. opacus 1b, 18, DSM 43205, ATCC 51881, ... (type strain)
R. opacus DSM 1069
R. opacus C125, DSM 8531
R. opacus DSM 43206, ATCC 21499, DSM 441, ...
R. opacus DSM 43250, ATCC 51882
R. opacus DSM 43251
R. opacus Pellegrino 327, DSM 43252, CIP ...
R. opacus S676, 225, DSM 43280, ATCC 4275, ...
R. opacus 691-19, DSM 43943, IMET 7363
R. opacus DSM 44072, CBS 330.61
R. opacus DSM 44236
R. opacus MoAcy1, DSM 44186
R. opacus Tue Acy 1, DSM 44188
R. opacus 305-97, L2aT3, PWD5, DSM 44304
R. opacus 307-97, PWD7, S2aT5, S₂aT5, ...
R. opacus 310-97, L1aT1, PWD10, DSM 44307
R. opacus GW-86a, DSM 44311
R. opacus Bie-17, DSM 44312
R. opacus 304-97, H1aT2, PWD4, DSM 44313
R. opacus 315-97, PWD15, S1aT1, DSM 44314
R. opacus 97-319, H3aT3, PWD19, DSM 44315
R. opacus ISO-5, DSM 44600
R. opacus ROL1, DSM 44853
R. opacus ROL2, DSM 44854
R. opacus R1L6, DSM 44855
R. opacus DSM 46027, IMET 7365, NCIB 9702
R. opacus ST033313(HKI),
R. opacus R7, CIP 107348
R. opacus GK 3, GK3, CIP 110889, CCMM B1295

Section

Rhodococcus opacus 1CP is a mesophilic prokaryote that was isolated from enrichment culture.

mesophilic
16S sequence
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Genus	*Rhodococcus*
	Species	**Rhodococcus opacus**
	Full Scientific Name (LPSN)	Rhodococcus opacus Klatte et al. 1995

	Synonym	Rhodococcus imtechensis
	Synonym	Rhodococcus percolatus

Information on culture and growth conditions Culture and growth conditions

[Ref.: #67321]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #67321]

Culture medium growth

[Ref.: #67321]

Culture medium link

Medium recipe at MediaDive

[Ref.: #67321]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #67321]			growth	28 °C

[Ref.: #67321]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #67321]	24941	+	-	-	-	-	-	-	+	+	+/-	+/-	+	+	+/-	+	-	+	+	+	+	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #67321]	14216	-	+	+/-	+/-	-	+	+	+/-	-	-	+	+/-	+/-	+	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67321]	Sample type/isolated from	enrichment culture
[Ref.: #67321]	Geographic location (country and/or sea, region)	Moscow Region
[Ref.: #67321]	Country	Russia
[Ref.: #67321]	Country ISO 3 Code	RUS
[Ref.: #67321]	Continent	Europe
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence Y11893 (>99% sequence identity) for Rhodococcus from Microbeatlas

Aquatic Samples	116
Soil Samples	425
Animal Samples	152
Plant Samples	113
Total Samples	806

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67321]	R.opacus 16S rRNA gene, strain 1CP	Y11893	1485	ENA	37919 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Rhodococcus opacus 1CP	GCA_001685605	complete	GenBank	37919 tax ID	*
[Ref.: #66792]	Rhodococcus opacus 1CP	2856586077	complete	JGI IMG/M	37919 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Rhodococcus opacus 1CP NCBI: GCA_001685605.1
[Ref.: #69480]	flagellated	no	98.235	no
[Ref.: #69480]	gram-positive	yes	80.629	no
[Ref.: #69480]	anaerobic	no	99.062	no
[Ref.: #69480]	aerobic	yes	88.072	no
[Ref.: #69480]	halophile	no	87.554	no
[Ref.: #69480]	spore-forming	no	61.473	no
[Ref.: #69480]	glucose-util	yes	90.378	yes
[Ref.: #69480]	thermophile	no	99.462	no
[Ref.: #69480]	motile	no	92.754	no
[Ref.: #69480]	glucose-ferment	no	90.154	no

Availability in culture collections External links

[Ref.: #67321]

Culture collection no.

DSM 46757, VKM Ac-2638

[Ref.: #112662]

SI-ID 396785

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67321

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 46757

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#112662

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID396785.1 )

* These data were automatically processed and therefore are not curated

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