Name: Mycobacterium smegmatis 607
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8319

Type strain:

Species: Mycobacterium smegmatis

Strain Designation: 607

Culture col. no.: DSM 43465, ATCC 607, NRRL B-692, SN 10, JCM 20379, IAM 12065, IFO 3207, NBRC 3207, CIP 110083

Strain history: CIP <- 2009, DSMZ <- I. Tarnok: strain SN10 <- Daddi, Milan, Italy (M. tuberculosis): strain 607 <- ATCC <- R.D. Herrold <- F. Novy

NCBI tax ID(s): 1772 (species)

SI-ID 3624

LMG 10267, ATCC 607, NRRL B-692, CCUG 21816, CCUG 21819, CCM 4622, NCTC 7017, CECT 3032, KCTC 2002, IAM 12065, CDBB 1096, CGMCC 1.0562, JCM 20379, DSM 43465, CIP 110083

Other strains from Mycobacterium smegmatis

Section

Mycobacterium smegmatis 607 is a facultative anaerobe, mesophilic, Gram-positive human pathogen that was isolated from Human.

Gram-positive
rod-shaped
facultative anaerobe
human pathogen
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium smegmatis**
	Full Scientific Name (LPSN)	Mycobacterium smegmatis (Trevisan 1889) Lehmann and Neumann 1899 (Approved Lists 1980)

	Synonym	"Bacillus* smegmatis"*
	Synonym	Mycolicibacterium smegmatis

Information on morphological and physiological properties Morphology

[Ref.: #122644]	Gram stain	positive

[Ref.: #122644]	Cell shape	rod-shaped

[Ref.: #122644]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11040]

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

[Ref.: #11040]

Culture medium growth

[Ref.: #11040]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11040]

Culture medium composition

expand / minimize

[Ref.: #36869]

Culture medium

MEDIUM 55 - for Mycobacterium

[Ref.: #36869]

Culture medium growth

[Ref.: #122644]

Culture medium

CIP Medium 72

[Ref.: #122644]

Culture medium growth

[Ref.: #122644]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #122644]

Culture medium

CIP Medium 55

[Ref.: #122644]

Culture medium growth

[Ref.: #122644]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #11040]	growth	37 °C
[Ref.: #36869]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #11040]	Temperature range	mesophilic
[Ref.: #36869]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #122644]

Oxygen tolerance

facultative anaerobe

[Ref.: #67770]

Observation

Assay of Antibiotics (Japanese Pharmacopoeia, 14 ed.)

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #122644]		nitrate ChEBI	+	reduction
[Ref.: #122644]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #122644]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122644]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122644]	oxidase	-
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #122644]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122644]	12348	-	-	+	+	+	+	+	+	+	-	+	+	-	-	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Human

[Ref.: #122644]	Sample type/isolated from	Original Koch strain of Bacillus tuberculosis as reported initially by Dr. Herrold. Avirulent and host for multiple mycobacteriophages.
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11040]	Pathogenicity (human)	yes
[Ref.: #11040]	Pathogenicity (animal)	yes
[Ref.: #11040]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #122644]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Mycobacterium smegmatis ATCC 607 16S rRNA gene, 5' untranslated region	U07954	204	ENA	1772 tax ID	*
[Ref.: #20218]	Mycobacterium smegmatis ATCC 607 intergenic region between 16S and 23S rRNA genes	U07955	561	ENA	1772 tax ID	*

Availability in culture collections External links

[Ref.: #11040]

Culture collection no.

DSM 43465, ATCC 607, NRRL B-692, SN 10, JCM 20379, IAM 12065, IFO 3207, NBRC 3207, CIP 110083

[Ref.: #77721]

SI-ID 3624

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Pathogenicity	Application of Mycobacterium smegmatis as a surrogate to evaluate drug leads against Mycobacterium tuberculosis.	Lelovic N, Mitachi K, Yang J, Lemieux MR, Ji Y, Kurosu M	J Antibiot (Tokyo)	10.1038/s41429-020-0320-7	2020	*
Enzymology	A New Citrinin Derivative from the Indonesian Marine Sponge-Associated Fungus Penicillium citrinum.	Sabdaningsih A, Liu Y, Mettal U, Heep J, Riyanti, Wang L, Cristianawati O, Nuryadi H, Triandala Sibero M, Marner M, Radjasa OK, Sabdono A, Trianto A, Schaberle TF	Mar Drugs	10.3390/md18040227	2020	*
Pathogenicity	Mycelial Growth and Antimicrobial Activity of Pleurotus Species (Agaricomycetes).	Castillo TA, Lemos RA, Pereira JRG, Alves JMA, Teixeira MFS	Int J Med Mushrooms	10.1615/IntJMedMushrooms.2018025477	2018	*
Pathogenicity	Mycelial Growth and Antimicrobial Activity of Species of Genus Lentinus (Agaricomycetes) from Brazil.	Castillo TA, Pereira JRG, Alves JMA, Teixeira MFS	Int J Med Mushrooms	10.1615/IntJMedMushrooms.2017024708	2017	*
Pathogenicity	Antimycobacterial evaluation and preliminary phytochemical investigation of selected medicinal plants traditionally used in Mozambique.	Luo X, Pires D, Ainsa JA, Gracia B, Mulhovo S, Duarte A, Anes E, Ferreira MJ	J Ethnopharmacol	10.1016/j.jep.2011.04.062	2011	*
Pathogenicity	IS1096-mediated DNA rearrangements play a key role in genome evolution of Mycobacterium smegmatis.	Wang XM, Galamba A, Warner DF, Soetaert K, Merkel JS, Kalai M, Bifani P, Lefevre P, Mizrahi V, Content J	Tuberculosis (Edinb)	10.1016/j.tube.2008.02.003	2008	*
Metabolism	Spontaneous transposition of IS1096 or ISMsm3 leads to glycopeptidolipid overproduction and affects surface properties in Mycobacterium smegmatis.	Kocincova D, Singh AK, Beretti JL, Ren H, Euphrasie D, Liu J, Daffe M, Etienne G, Reyrat JM	Tuberculosis (Edinb)	10.1016/j.tube.2008.02.005	2008	*
Pathogenicity	Synthesis of caprazamycin analogues and their structure--activity relationship for antibacterial activity.	Hirano S, Ichikawa S, Matsuda A	J Org Chem	10.1021/jo702264e	2007	*
Metabolism	The cell envelope structure and properties of Mycobacterium smegmatis mc(2)155: is there a clue for the unique transformability of the strain?	Etienne G, Laval F, Villeneuve C, Dinadayala P, Abouwarda A, Zerbib D, Galamba A, Daffe M	Microbiology (Reading)	10.1099/mic.0.27869-0	2005	*
Enzymology	Glycine and alanine dehydrogenase activities are catalyzed by the same protein in Mycobacterium smegmatis: upregulation of both activities under microaerophilic adaptation.	Usha V, Jayaraman R, Toro JC, Hoffner SE, Das KS	Can J Microbiol	10.1139/w01-126	2002	*
Phylogeny	[Properties of MTPN11 mycobacteriophage].	Zhilenkov EL, Shemiakin IG, Stepanshina VN, Korobova OV, Oborotov MV, Dorozhkova IR	Mikrobiologiia		1998	*
Metabolism	Exochelin genes in Mycobacterium smegmatis: identification of an ABC transporter and two non-ribosomal peptide synthetase genes.	Zhu W, Arceneaux JE, Beggs ML, Byers BR, Eisenach KD, Lundrigan MD	Mol Microbiol	10.1046/j.1365-2958.1998.00961.x	1998	*
Enzymology	Ca2+/calmodulin dependent protein kinase from Mycobacterium smegmatis ATCC 607.	Sharma S, Giri S, Khuller GK	Mol Cell Biochem	10.1023/a:1006883006402	1998	*
Metabolism	Enhanced hydrogen peroxide sensitivity and altered stress protein expression in iron-starved Mycobacterium smegmatis.	Lundrigan MD, Arceneaux JE, Zhu W, Byers BR	Biometals	10.1023/a:1018355928990	1997	*
Pathogenicity	Role of cyclic adenosine monophosphate in phospholipid synthesis in Mycobacterium smegmatis ATCC 607.	Kaur H, Khuller GK	Lipids	10.1007/BF02536043	1995	*
Pathogenicity	Synthesis of some hydroxamic acid derivatives of benzimidazole and their antibacterial and antifungal activities.	Gunes HS, Cosar G	Arzneimittelforschung		1992	*
Metabolism	Effect of ethambutol on the phospholipids of ethambutol susceptible and resistant strains of Mycobacterium smegmatis ATCC 607.	Sareen M, Khuller GK	Indian J Biochem Biophys		1990	*
Pathogenicity	Mode of action of deoxypheganomycin D on Mycobacterium smegmatis ATCC 607.	Suzukake-Tsuchiya K, Hori M, Shimada N, Hamada M	J Antibiot (Tokyo)	10.7164/antibiotics.41.675	1988	*
Pathogenicity	On the possibility of cross-resistance between rifampicin and clofazimine in mycobacteria--a laboratory study.	Urbanczik R	Zentralbl Bakteriol Mikrobiol Hyg A	10.1016/s0176-6724(85)80106-1	1985	*
Metabolism	Cerulenin inhibition of lipid synthesis and its reversal by exogenous fatty acids in Mycobacterium smegmatis ATCC 607.	Mahajan S, Khuller GK	Can J Biochem Cell Biol	10.1139/o85-012	1985	*
Metabolism	Cerulenin effect on phospholipid metabolism in Mycobacterium smegmatis ATCC 607.	Mahajan S, Khuller GK	Biochim Biophys Acta	10.1016/0005-2760(84)90178-4	1984	*
Metabolism	Macromolecular biosynthesis in Mycobacterium smegmatis ATCC 607 in the presence of antibodies to mannophosphoinositides.	Khuller GK, Dogra A, Malik U, Asotra S	J Gen Microbiol	10.1099/00221287-130-7-1795	1984	*
Phylogeny	Isolation of mycobacterial phage from the laboratory strain Mycobacterium leprae murium "Douglas".	Sula L, Sulova J, Matejka M, Malkova J	Czech Med		1984	*
Metabolism	Subcellular distribution of mannophosphoinositides in Mycobacterium smegmatis during growth.	Penumarti N, Khuller GK	Experientia	10.1007/BF01990417	1983	*
Enzymology	Structural elucidation by field desorption and electron-impact mass spectrometry of the C-mycosides isolated from Mycobacterium smegmatis.	Daffe M, Laneelle MA, Puzo G	Biochim Biophys Acta	10.1016/0005-2760(83)90304-1	1983	*
Cultivation	Effect of fatty acid supplementation on the lipid composition of Mycobacterium smegmatis ATCC 607, grown at 27 degrees and 37 degrees C.	Khuller GK, Taneja R, Nath N	J Appl Bacteriol	10.1111/j.1365-2672.1983.tb01301.x	1983	*
Metabolism	Inhibitory activity and mode of action of diaminodiphenylsulfone in cell-free folate-synthesizing systems prepared from Mycobacterium lufu and Mycobacterium leprae. A comparison.	Kulkarni VM, Seydel JK	Chemotherapy	10.1159/000238174	1983	*
Metabolism	Influence of antibodies to mannophosphoinositides on phospholipid synthesis in Mycobacterium smegmatis ATCC 607.	Penumarti N, Khuller GK	Infect Immun	10.1128/iai.37.3.884-890.1982	1982	*
Metabolism	Biosynthesis of hexamannophosphoinositides in Mycobacterium smegmatis ATCC 607.	Nalini P, Khuller GK	Arch Microbiol	10.1007/BF00690824	1982	*
Pathogenicity	Correlations between structure and antimycobacterial activity in a series of 2-acetylpyridine thiosemicarbazones.	Collins FM, Klayman DL, Morrison NE	J Gen Microbiol	10.1099/00221287-128-6-1349	1982	*
Metabolism	Evidence for plasmid-mediated restriction-modification in Mycobacterium avium intracellulare.	Crawford JT, Cave MD, Bates JH	J Gen Microbiol	10.1099/00221287-127-2-333	1981	*
Pathogenicity	Biochemical study of R-75-1, a new semi-synthetic rifamycin.	Xiao-Chang F, Uehara Y, Abe Y, Hori M, Hamada M, Umezawa H	J Antibiot (Tokyo)	10.7164/antibiotics.34.713	1981	*
Metabolism	Effect of glycine on phospholipids of Mycobacterium smegmatis ATCC 607.	Subramoniam A, Subrahmanyam D	J Gen Microbiol	10.1099/00221287-124-1-203	1981	*
Metabolism	Phospholipid metabolism in Mycobacterium smegmatis ATCC 607 grown at 37 degrees and 27 C degrees C.	Taneja R, Khuller GK	Arch Microbiol	10.1007/BF00417185	1981	*
Metabolism	Effect of growth temperature on the lipid composition of Mycobacterium smegmatis ATCC 607.	Taneja R, Malik U, Khuller GK	J Gen Microbiol	10.1099/00221287-113-2-413	1979	*
Pathogenicity	Resistance relationships in Mycobacterium smegmatis ATCC 607 to phages sensitive or resistant to both chloroform and streptomycin sulphate.	Jones WD Jr, Greenberg J	J Gen Virol	10.1099/0022-1317-39-3-555	1978	*
Pathogenicity	Antitubercular 2,8-bis(alkylaminomethyl)phenazines.	Murdock KC, Lin Y, Thomas JP, Lang SA Jr	J Med Chem	10.1021/jm00202a020	1978	*
Pathogenicity	In vitro cytotoxicity of peritoneal macrophages activated with Mycobacterium smegmatis.	Yamamoto S, Tokunaga T	Microbiol Immunol	10.1111/j.1348-0421.1978.tb00345.x	1978	*
Metabolism	Permeability barrier to rifampin in mycobacteria.	Hui J, Gordon N, Kajioka R	Antimicrob Agents Chemother	10.1128/AAC.11.5.773	1977	*
Enzymology	Enzymatic synthesis of 2-O-alpha-D-mannopyranosyl-methyl-alpha-D-mannopyranoside by a cell-free particulate system of Mycobacterium smegmatis.	Schultz JC, Takayama K	Biochim Biophys Acta	10.1016/0304-4165(76)90184-7	1976	*
Metabolism	Evidence for host-dependent modification and restriction of bacteriophage DNA in Mycobacterium tuberculosis.	Rado TA, Bates JH, Fitzhugh JK	J Gen Virol	10.1099/0022-1317-30-1-91	1976	*
Phylogeny	Nature of the receptor substance of Mycobacterium smegmatis for D4 bacteriophage adsorption.	Furuchi A, Tokunaga T	J Bacteriol	10.1128/jb.111.2.404-411.1972	1972	*
Phylogeny	Seven New Alkaloids Isolated from Marine Flavobacterium Tenacibaculum discolor sv11.	Wang L, Marner M, Mettal U, Liu Y, Schaberle TF	Mar Drugs	10.3390/md20100620	2022	*
	Discovery and Biosynthesis of Antimicrobial Phenethylamine Alkaloids from the Marine Flavobacterium Tenacibaculum discolor sv11.	Wang L, Linares-Otoya V, Liu Y, Mettal U, Marner M, Armas-Mantilla L, Willbold S, Kurtan T, Linares-Otoya L, Schaberle TF	J Nat Prod	10.1021/acs.jnatprod.1c01173	2022	*
	Dehydroabietic Acid Microencapsulation Potential as Biofilm-Mediated Infections Treatment.	Neto I, Dominguez-Martin EM, Ntungwe E, Reis CP, Pesic M, Faustino C, Rijo P	Pharmaceutics	10.3390/pharmaceutics13060825	2021	*
	In vitro evaluation of antimicrobial features of vasopressors.	Bostan H, Tomak Y, Karaoglu SA, Erdivanli B, Hanci V	Braz J Anesthesiol	10.1016/j.bjane.2013.02.002	2013	*
	Light-induced changes in the phospholipid composition of Mycobacterium smegmatis ATCC 607.	Subramoniam A, Subrahmanyam D	J Gen Microbiol	10.1099/00221287-128-2-419	1982	*

References

#11040

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43465

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36869 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#77721

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID3624.1 )

#122644 Collection of Institut Pasteur ; Curators of the CIP; CIP 110083
* These data were automatically processed and therefore are not curated

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