Mycobacterium smegmatis (CIP 56.5, CCTM 170, NCTC 53)

Name: Mycobacterium smegmatis (CIP 56.5, CCTM 170, NCTC 53)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 136747

Type strain

Culture col. no. CIP 56.5 CCTM 170 NCTC 53

NCBI tax ID(s) 1772

Special Collections CIP

Links

Mycobacterium smegmatis CIP 56.5 is an obligate aerobe, mesophilic prokaryote of the family Mycobacteriaceae.

obligate aerobe mesophilic

Name and taxonomic classification

SI-ID 69396

NCTC 53,CIP 56.5

@ref 20215

Last LPSN update 2025-12-16 10:02:39

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Mycobacteriaceae

Genus Mycobacterium

Species Mycobacterium smegmatis

Full scientific name Mycobacterium smegmatis (Trevisan 1889) Lehmann and Neumann 1899 (Approved Lists 1980)

Synonyms (2)

"Bacillus smegmatis"
Mycolicibacterium smegmatis

BacDive ID	Other strains from **Mycobacterium smegmatis** (27)	Type strain
8324	M. smegmatis GA 735, Cornell 3, 14 133 0010, DSM 43756, ... (type strain)
8256	M. smegmatis Minetti 41, DSM 43479, SN 220
8309	M. smegmatis DSM 43059, CCM 1889, IMRU N 525, NCTC 525, ...
8310	M. smegmatis DSM 43061, CCM 2228, NCTC 523
8311	M. smegmatis 52-L-1, DSM 43078, IMRU 672
8312	M. smegmatis DSM 43079, IMRU 853
8313	M. smegmatis DSM 43080, IMRU 580
8314	M. smegmatis 52-Q-1, DSM 43081, IMRU 595
8315	M. smegmatis DSM 43277, ATCC 101, ATCC 14468, NCIB 9953, ...
8316	M. smegmatis 74C, Schering 250, DSM 43286, ATCC 356, NCTC ...
8317	M. smegmatis 169, Schering 275, DSM 43299, ATCC 114, CCUG ...
8318	M. smegmatis DSM 43464, ATCC 27204, IMET 10646, SN 2
8319	M. smegmatis 607, DSM 43465, ATCC 607, NRRL B-692, SN ...
8320	M. smegmatis DSM 43466, IMRU 7992, SN 35
8321	M. smegmatis DSM 43467, IMRU 9822, SN 44
8322	M. smegmatis 114, DSM 43468, ATCC 27205, IMET 10647, IMRU ...
8323	M. smegmatis 7449, DSM 43469, IMET 10648, SN 46
8325	M. smegmatis DSM 44200, HKI 0056 (SG 987)
8326	M. smegmatis 987-M10, DSM 44201, HKI 0062
104243	M. smegmatis ST033288(HKI),
139485	M. smegmatis F21, CIP 73.26
143909	M. smegmatis CCUG 21821, ATCC 19979, NCTC 333
143910	M. smegmatis CCUG 21822, ATCC 23028
143911	M. smegmatis CCUG 21823, ATCC 23032
143912	M. smegmatis CCUG 21824, ATCC 23037
145167	M. smegmatis CCUG 27786
145276	M. smegmatis CCUG 28062, NCTC 3821

Morphology

Cell morphology

36287

Motilityno

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link
36287	MEDIUM 55 - for Mycobacterium
36287	CIP Medium 55		Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
36287	positive	growth	37	mesophilic
36287	positive	growth	25-45
36287	negative	growth	10

Physiology and metabolism

Oxygen tolerance

36287

Oxygen toleranceobligate aerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
36287	NaCl	positive	growth	0-6 %
36287	NaCl		growth	8 %
36287	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
36287	16947 ChEBI	citrate	-	carbon source
36287	4853 ChEBI	esculin	-	hydrolysis
36287	606565 ChEBI	hippurate	-	hydrolysis
36287	17632 ChEBI	nitrate	+	reduction
36287	17632 ChEBI	nitrate	-	respiration
36287	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
36287	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
36287	15688 ChEBI	acetoin	-
36287	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
36287	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
36287	amylase	-
36287	beta-galactosidase	-	3.2.1.23
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
36287	caseinase	-	3.4.21.50
36287	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
36287	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
36287	gamma-glutamyltransferase	-	2.3.2.2
36287	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
36287	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
36287	ornithine decarboxylase	-	4.1.1.17
36287	oxidase	-
36287	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	+	3.4.21.4	from API zym
36287	tryptophan deaminase	-
36287	tween esterase	+
36287	urease	+	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
36287	-	-	+	+	-	+	+	+	+	-	+	+	-	-	-	-	+	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
36287	not determinedn.d.	-	+	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	+	-	+	-	+	+	+	-	-	+	+	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	+	-	-	-	-	-	-	+	-	-	+	-	+	-	-	+	-	-	-	-	-	-	+	-	-	-	-	-	+	-	+	-	-	-	-	+	+	-	-

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
36287	1	Risk group (French classification)

Sequence information

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Characterization of mutations in Mycobacterium smegmatis involved in resistance to fluoroquinolones.	Revel V, Cambau E, Jarlier V, Sougakoff W.	Antimicrob Agents Chemother	10.1128/aac.38.9.1991	1994

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#36287	Collection of Institut Pasteur ; Curators of the CIP; CIP 56.5
#68382	Automatically annotated from API zym .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mycobacterium smegmatis (CIP 56.5, CCTM 170, NCTC 53)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

Genome-based predictions

Literature

Literature

References

BacDive

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