Name: Mycobacterium smegmatis GA 735
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8324

Type strain:

Species: Mycobacterium smegmatis

Strain Designation: GA 735, Cornell 3

Culture col. no.: DSM 43756, ATCC 19420, JCM 5866, KCTC 9108, NCTC 8159, CCUG 21002, CCUG 21815, CECT 3020, CIP 104444, JCM 6386, NRRL B-14616, VKM Ac-1239

Strain history: NCTC 8159 <-- R. E. Gordon <-- Hagan <-- Alvarez and Tavel strain Cornell 3.

NCBI tax ID(s): 1772 (species)

Other strains from Mycobacterium smegmatis

Section

Mycobacterium smegmatis GA 735 is an aerobe, mesophilic, Gram-positive human pathogen of the family Mycobacteriaceae.

Gram-positive
aerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium smegmatis**
	Full Scientific Name (PNU)	Mycobacterium smegmatis (Trevisan 1889) Lehmann and Neumann 1899 (Approved Lists 1980)

	Synonym	"Bacillus* smegmatis"*
	Synonym	Mycolicibacterium smegmatis

Information on morphological and physiological properties Morphology

[Ref.: #67771]

Gram stain

positive

Multimedia content

[Ref.: #11178]

Intellectual property rights:

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Caption:

Medium 65 37°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium composition

expand / minimize

[Ref.: #41725]

Culture medium

MEDIUM 55 - for Mycobacterium

[Ref.: #41725]

Culture medium growth

[Ref.: #11178]

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

[Ref.: #11178]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #11178]	growth	37 °C
[Ref.: #41725]	growth	37 °C
[Ref.: #67770]	growth	37 °C
[Ref.: #67771]	growth	37 °C

[Ref.: #11178]	Temperature range	mesophilic
[Ref.: #41725]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

aerobe

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11178]	Geographic location (country and/or sea, region)	Plant Industry Station, Beltsville, Maryland
[Ref.: #11178]	Country	USA
[Ref.: #11178]	Country ISO 3 Code	USA
[Ref.: #11178]	Continent	North America
[Ref.: #11178]	Geographic location	39.0358°/-76.9175°
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11178]	Pathogenicity (human)	yes
[Ref.: #11178]	Pathogenicity (animal)	yes
[Ref.: #11178]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Mycobacterium smegmatis strain ATCC19420 16S ribosomal RNA (rrs) gene, partial sequence	AF059846	175	ENA	1772 tax ID	*
[Ref.: #20218]	Mycobacterium smegmatis 16S rRNA gene, strain ATCC 19420	AJ131761	1487	ENA	1772 tax ID	*
[Ref.: #20218]	Mycobacterium smegmatis 16S rRNA gene	AJ536041	1461	ENA	1772 tax ID	*
[Ref.: #20218]	Mycobacterium smegmatis strain ATCC 19420 16S ribosomal RNA gene, partial sequence	AY457078	1487	ENA	1772 tax ID	*
[Ref.: #20218]	Mycobacterium smegmatis strain DSM 43756 16S ribosomal RNA gene, partial sequence	KF410356	1368	ENA	1772 tax ID	*
[Ref.: #20218]	M.smegmatis (NCTC 8159) gene for 16S ribosomal RNA partial (207bp)	X76255	207	ENA	1772 tax ID	*
[Ref.: #20218]	M.smegmatis (NCTC 8159) gene for 16S ribosomal RNA partial (238bp)	X76256	238	ENA	1772 tax ID	*
[Ref.: #20218]	M.smegmatis (NCTC 8159) genes for 16S and 23s ribosomal RNA partial (381bp)	X76257	381	ENA	1772 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mycolicibacterium smegmatis NCTC8159	GCA_001457595	complete	GenBank	1772 tax ID	*
[Ref.: #66792]	Mycobacterium smegmatis strain NCTC8159	1772.19	complete	PATRIC	1772 tax ID	*
[Ref.: #66792]	Mycolicibacterium smegmatis NCTC8159	2657245626	complete	JGI IMG/M	1772 tax ID	*
[Ref.: #67770]	Mycobacterium smegmatis genome assembly NCTC8159, chromosome : 1	LN831039		ENA	1772 tax ID
[Ref.: #66792]	Streptococcus pneumoniae GPSC19 substr. ST433	GCA_900059275	scaffold	PATRIC	1313 tax ID	*

[Ref.: #67770]

GC-content

67.3 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #11178]

Culture collection no.

DSM 43756, ATCC 19420, JCM 5866, KCTC 9108, NCTC 8159, CCUG 21002, CCUG 21815, CECT 3020, CIP 104444, JCM 6386, NRRL B-14616, VKM Ac-1239

[Ref.: #20218]

Associated Passport(s) in StrainInfo

153068, 100120, 139668, 168635, 100121

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Cloning and sequencing of the gene which encodes the highly inducible acetamidase of Mycobacterium smegmatis.	Mahenthiralingam E, Draper P, Davis EO, Colston MJ	J Gen Microbiol	10.1099/00221287-139-3-575	1993	*
Metabolism	Structure determination of ribosylated rifampicin and its derivative: new inactivated metabolites of rifampicin by mycobacterial strains.	Morisaki N, Kobayashi H, Iwasaki S, Furihata K, Dabbs ER, Yazawa K, Mikami Y	J Antibiot (Tokyo)	10.7164/antibiotics.48.1299	1995	*
Genetics	Regulation of the inducible acetamidase gene of Mycobacterium smegmatis.	Parish T, Mahenthiralingam E, Draper P, Davis EO, Colston EO	Microbiology (Reading)	10.1099/00221287-143-7-2267	1997	*
Metabolism	ADP-ribosylation as an intermediate step in inactivation of rifampin by a mycobacterial gene.	Quan S, Imai T, Mikami Y, Yazawa K, Dabbs ER, Morisaki N, Iwasaki S, Hashimoto Y, Furihata K	Antimicrob Agents Chemother	10.1128/AAC.43.1.181	1999	*
Enzymology	Pathogenicity of Mycobacterium fortuitum and Mycobacterium smegmatis to goldfish, Carassius auratus.	Talaat AM, Trucksis M, Kane AS, Reimschuessel R	Vet Microbiol	10.1016/s0378-1135(99)00002-4	1999	*
Phylogeny	Mycobacterium wolinskyi sp. nov. and Mycobacterium goodii sp. nov., two new rapidly growing species related to Mycobacterium smegmatis and associated with human wound infections: a cooperative study from the International Working Group on Mycobacterial Taxonomy.	Brown BA, Springer B, Steingrube VA, Wilson RW, Pfyffer GE, Garcia MJ, Menendez MC, Rodriguez-Salgado B, Jost KC Jr, Chiu SH, Onyi GO, Bottger EC, Wallace RJ Jr	Int J Syst Bacteriol	10.1099/00207713-49-4-1493	1999	*
Metabolism	Identification and characterization of a new intermediate in the ribosylative inactivation pathway of rifampin by Mycobacterium smegmatis.	Imai T, Watanabe K, Mikami Y, Yazawa K, Ando A, Nagata Y, Morisaki N, Hashimoto Y, Furihata K, Dabbs ER	Microb Drug Resist	10.1089/mdr.1999.5.259	1999	*
Metabolism	Structures of ADP-ribosylated rifampicin and its metabolite: intermediates of rifampicin-ribosylation by Mycobacterium smegmatis DSM43756.	Morisaki N, Hashimoto Y, Furihata K, Imai T, Watanabe K, Mikami Y, Yazawa K, Ando A, Nagata Y, Dabbs ER	J Antibiot (Tokyo)	10.7164/antibiotics.53.269	2000	*
Pathogenicity	Characterization of In53, a class 1 plasmid- and composite transposon-located integron of Escherichia coli which carries an unusual array of gene cassettes.	Naas T, Mikami Y, Imai T, Poirel L, Nordmann P	J Bacteriol	10.1128/JB.183.1.235-249.2001	2001	*
Enzymology	Panniculitis, due to Mycobacterium smegmatis, in two Finnish cats.	Alander-Damsten YK, Brander EE, Paulin LG	J Feline Med Surg	10.1053/jfms.2001.0189	2003	*
Genetics	A rifampin-hypersensitive mutant reveals differences between strains of Mycobacterium smegmatis and presence of a novel transposon, IS1623.	Alexander DC, Jones JR, Liu J	Antimicrob Agents Chemother	10.1128/AAC.47.10.3208-3213.2003	2003	*
Pathogenicity	New quinolin-4-yl-1,2,3-triazoles carrying amides, sulphonamides and amidopiperazines as potential antitubercular agents.	Thomas KD, Adhikari AV, Chowdhury IH, Sumesh E, Pal NK	Eur J Med Chem	10.1016/j.ejmech.2011.03.039	2011	*
Pathogenicity	Acanthamoeba polyphaga-enhanced growth of Mycobacterium smegmatis.	Lamrabet O, Mba Medie F, Drancourt M	PLoS One	10.1371/journal.pone.0029833	2012	*
Enzymology	Crystal structure and biochemical characterization of O-acetylhomoserine acetyltransferase from Mycobacterium smegmatis ATCC 19420.	Sagong HY, Hong J, Kim KJ	Biochem Biophys Res Commun	10.1016/j.bbrc.2019.07.117	2019	*

References

#11178

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43756

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#41725 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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