Mycobacterium smegmatis (DSM 43756, ATCC 19420, JCM 5866)

Name: Mycobacterium smegmatis (DSM 43756, ATCC 19420, JCM 5866)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 8324

Type strain

Strain Designation GA 735, Cornell 3, 14 133 0010

Culture col. no. DSM 43756 ATCC 19420 JCM 5866 KCTC 9108 NCTC 8159 10 more

NCBI tax ID(s) 1772

Special Collections DSMZ CCUG JCM KCTC CIP

History <- NCTC; NCTC 8159 <- R. E. Gordon; Cornell 3 <- JCM <- NCTC <- R. Gordon <- Hagan <- Alvarez and Tavel (Cornell 3) NCTC 8159 <-- R. E. Gordon <-- Hagan <-- Alvarez and Tavel strain Cornell 3. CIP <- 1995, V. Vincent, Inst. Pasteur, Paris, France: strain 14 133 0010 <- 1981, ATCC <- NCTC <- R.E. Gordon <- Hagan <- Alvarez and Tavel: strain Cornell 3

Links

Mycobacterium smegmatis GA 735 is an aerobe, Gram-positive human pathogen of the family Mycobacteriaceae.

Gram-positive aerobe human pathogen genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 36980

ATCC 19420,NCTC 8159,CCUG 21002,CCUG 21815,JCM 5866,CIP 104444,DSM 43756,KCTC 9108,VKM Ac-1239,CECT 3020,JCM 6386,NRRL B-14616,CNCTC 7294

@ref 20215

Last LPSN update 2026-02-09 11:19:41

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Mycobacteriaceae

Genus Mycobacterium

Species Mycobacterium smegmatis

Full scientific name Mycobacterium smegmatis (Trevisan 1889) Lehmann and Neumann 1899 (Approved Lists 1980)

Synonyms (2)

"Bacillus smegmatis"
Mycolicibacterium smegmatis

BacDive ID	Other strains from **Mycobacterium smegmatis** (27)	Type strain
8256	M. smegmatis Minetti 41, DSM 43479, SN 220
8309	M. smegmatis DSM 43059, CCM 1889, IMRU N 525, NCTC 525, ...
8310	M. smegmatis DSM 43061, CCM 2228, NCTC 523
8311	M. smegmatis 52-L-1, DSM 43078, IMRU 672
8312	M. smegmatis DSM 43079, IMRU 853
8313	M. smegmatis DSM 43080, IMRU 580
8314	M. smegmatis 52-Q-1, DSM 43081, IMRU 595
8315	M. smegmatis DSM 43277, ATCC 101, ATCC 14468, NCIB 9953, ...
8316	M. smegmatis 74C, Schering 250, DSM 43286, ATCC 356, NCTC ...
8317	M. smegmatis 169, Schering 275, DSM 43299, ATCC 114, CCUG ...
8318	M. smegmatis DSM 43464, ATCC 27204, IMET 10646, SN 2
8319	M. smegmatis 607, DSM 43465, ATCC 607, NRRL B-692, SN ...
8320	M. smegmatis DSM 43466, IMRU 7992, SN 35
8321	M. smegmatis DSM 43467, IMRU 9822, SN 44
8322	M. smegmatis 114, DSM 43468, ATCC 27205, IMET 10647, IMRU ...
8323	M. smegmatis 7449, DSM 43469, IMET 10648, SN 46
8325	M. smegmatis DSM 44200, HKI 0056 (SG 987)
8326	M. smegmatis 987-M10, DSM 44201, HKI 0062
104243	M. smegmatis ST033288(HKI),
136747	M. smegmatis CIP 56.5, CCTM 170, NCTC 53
139485	M. smegmatis F21, CIP 73.26
143909	M. smegmatis CCUG 21821, ATCC 19979, NCTC 333
143910	M. smegmatis CCUG 21822, ATCC 23028
143911	M. smegmatis CCUG 21823, ATCC 23032
143912	M. smegmatis CCUG 21824, ATCC 23037
145167	M. smegmatis CCUG 27786
145276	M. smegmatis CCUG 28062, NCTC 3821

Morphology

Cell morphology

@ref	Cell shape	Gram stain	Confidence
67771		positive
122643	rod-shaped
125439		positive	95.5

Multimedia

@ref:	11178
multimedia content:	DSM_43756-1.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43756-1.jpg
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

@ref:	11178
multimedia content:	DSM_43756.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43756.jpg
caption:	Medium 65 37°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11178	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
41725	MEDIUM 55 - for Mycobacterium
11178	MIDDLEBROOK MEDIUM (DSMZ Medium 645)	Medium recipe at MediaDive	Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
122643	CIP Medium 55	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
11178	positive	growth	37
41725	positive	growth	37
67770	positive	growth	37
67771	positive	growth	37
122643	positive	growth	30-45
122643	negative	growth	10

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
67771	aerobe
122643	obligate aerobe
125439	obligate aerobe	98.9

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
122643	4853 ChEBI	esculin	+	hydrolysis
122643	17632 ChEBI	nitrate	+	reduction
122643	17632 ChEBI	nitrate	-	respiration
122643	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
122643	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
122643	amylase	-
68382	beta-galactosidase	-	3.2.1.23	from API zym
122643	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
122643	caseinase	-	3.4.21.50
122643	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
122643	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
122643	gelatinase	-
122643	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
122643	lipase	+
68382	lipase (C 14)	+		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
122643	oxidase	-
68382	trypsin	+	3.4.21.4	from API zym
122643	tween esterase	+
122643	urease	+	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
122643	-	+	+	+	+	+	+	+	+	-	+	+	-	-	-	+	+	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
122643	not determinedn.d.	+	+	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	+	-	-	+	+	+	-	+	+	+	-	-	-	-	+	-	+	+	-	-	+	-	-	-	+	+	+	-	-	-	-	+	+	+	+	-	-	+	-	-	-	-	-	-	+	-	-	+	-	+	-	-	+	-	-	-	+	+	-	+	-	+	-	-	-	+	-	+	+	-	+	-	+	+	-	-

Isolation, sampling and environmental information

Isolation

@ref	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
11178	Plant Industry Station, Beltsville, Maryland	USA	USA	North America	39.0358	-76.9175 39.0358/-76.9175

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Pathogenicity animal	Biosafety level	Biosafety level comment
11178			2	Risk group (German classification) According to TRBA 466
122643			1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	NCTC8159 assembly for Mycolicibacterium smegmatis NCTC8159	complete	GCA_001457595	1772.19	2657245626	1772	98.15
66792	ASM2922201v1 assembly for Mycolicibacterium smegmatis ATCC 19420	contig	GCA_029222015	1772.122	8117802130	1772	13.64

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Mycolicibacterium smegmatis strain ATCC 19420 16S ribosomal RNA (rrs), partial sequence	AF059846	175	1772
20218	Mycobacterium smegmatis 16S rRNA gene, strain ATCC 19420	AJ131761	1487	1772
20218	Mycobacterium smegmatis 16S rRNA gene	AJ536041	1461	1772
20218	Mycolicibacterium smegmatis strain ATCC 19420 16S ribosomal RNA gene, partial sequence	AY457078	1487	1772
20218	Mycobacterium smegmatis strain DSM 43756 16S ribosomal RNA gene, partial sequence	KF410356	1368	1772
20218	M.smegmatis (NCTC 8159) gene for 16S ribosomal RNA partial (207bp)	X76255	207	1772
20218	M.smegmatis (NCTC 8159) gene for 16S ribosomal RNA partial (238bp)	X76256	238	1772
20218	M.smegmatis (NCTC 8159) genes for 16S and 23s ribosomal RNA partial (381bp)	X76257	381	1772
124043	Mycobacterium smegmatis strain CIP 104444 16S ribosomal RNA gene, partial sequence.	AF547967	544	1772

GC content

@ref	GC-content (mol%)	Method
67770	67.3	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Mycobacterium smegmatis strain NCTC8159
PATRIC: 1772.19

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	62.90	no
125439	motility	BacteriaNetⓘ	no	74.50	no
125439	gram_stain	BacteriaNetⓘ	positive	95.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Mycolicibacterium smegmatis NCTC8159
NCBI: GCA_001457595.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	88.68	yes
125438	anaerobic	anaerobicⓘ	no	94.70	no
125438	aerobic	aerobicⓘ	yes	89.61	yes
125438	spore-forming	spore-formingⓘ	no	60.49	no
125438	thermophilic	thermophileⓘ	no	97.00	yes
125438	flagellated	motile2+ⓘ	no	87.37	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Investigations of microbial adaptation to singular, binary, and fully formulated quaternary ammonium compounds.	Willmott T, Kelly PP, Jadaan L, Gifford DR, Mercer SD, Humphreys GJ, Knight CG, Lu JR, McBain AJ.	Appl Environ Microbiol	10.1128/aem.00666-24	2024
	Structure Elucidation and Biosynthesis of Nannosterols A and B, Myxobacterial Sterols from Nannocystis sp. MNa10993.	Akone SH, Hug JJ, Kaur A, Garcia R, Muller R.	J Nat Prod	10.1021/acs.jnatprod.2c01143	2023
	Thermal Inactivation of Mycobacterium avium subsp. paratuberculosis in Artificially Contaminated Milk by Direct Steam Injection.	Peterz M, Butot S, Jagadeesan B, Bakker D, Donaghy J.	Appl Environ Microbiol	10.1128/aem.04042-15	2016
	Three New Stigmatellin Derivatives Reveal Biosynthetic Insights of Its Side Chain Decoration.	Okoth DA, Hug JJ, Garcia R, Muller R.	Molecules	10.3390/molecules27144656	2022
Pathogenicity	Application of a microcalorimetric method for determining drug susceptibility in mycobacterium species.	Howell M, Wirz D, Daniels AU, Braissant O.	J Clin Microbiol	10.1128/jcm.05556-11	2012
Metabolism	Structure and biosynthesis of sorangipyranone - a new gamma-dihydropyrone from the myxobacterial strain MSr12020.	Okoth DA, Hug JJ, Mandi A, Kurtan T, Garcia R, Muller R.	J Ind Microbiol Biotechnol	10.1093/jimb/kuab029	2021
	Ribosylative inactivation of rifampin by Mycobacterium smegmatis is a principal contributor to its low susceptibility to this antibiotic.	Quan S, Venter H, Dabbs ER.	Antimicrob Agents Chemother	10.1128/aac.41.11.2456	1997
Pathogenicity	2-Hydroxysorangiadenosine: Structure and Biosynthesis of a Myxobacterial Sesquiterpene-Nucleoside.	Okoth DA, Hug JJ, Garcia R, Sproer C, Overmann J, Muller R.	Molecules	10.3390/molecules25112676	2020
Transcriptome	Nitrogen starvation-induced transcriptome alterations and influence of transcription regulator mutants in Mycobacterium smegmatis.	Jessberger N, Lu Y, Amon J, Titgemeyer F, Sonnewald S, Reid S, Burkovski A.	BMC Res Notes	10.1186/1756-0500-6-482	2013
Metabolism	The antimicrobial compound xantholysin defines a new group of Pseudomonas cyclic lipopeptides.	Li W, Rokni-Zadeh H, De Vleeschouwer M, Ghequire MG, Sinnaeve D, Xie GL, Rozenski J, Madder A, Martins JC, De Mot R.	PLoS One	10.1371/journal.pone.0062946	2013
Phylogeny	Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera.	Gupta RS, Lo B, Son J.	Front Microbiol	10.3389/fmicb.2018.00067	2018
	Diosgenin ameliorates silica-induced tuberculosis in rats.	Adu WA, Ativui S, Ofori M, Owusu G, Danquah CA, Poku Sampene Ossei P.	Animal Model Exp Med	10.1002/ame2.70055	2025
	Evaluation of Anti-Inflammatory and Anti-Tubercular Activity of 4-Methyl-7-Substituted Coumarin Hybrids and Their Structure Activity Relationships.	Nibin Joy M, Guda MR, Zyryanov GV.	Pharmaceuticals (Basel)	10.3390/ph16091326	2023
	High-efficiency production of 5-hydroxyectoine using metabolically engineered Corynebacterium glutamicum.	Jungmann L, Hoffmann SL, Lang C, De Agazio R, Becker J, Kohlstedt M, Wittmann C.	Microb Cell Fact	10.1186/s12934-022-02003-z	2022
	NMI-SO₂Cl₂-Mediated Amide Bond Formation: Facile Synthesis of Some Dihydrotriazolopyrimidine Amide Derivatives as Potential Anti-Inflammatory and Anti-Tubercular Agents.	Babu A, Sunil K, Sajith AM, Reddy EK, Santra S, Zyryanov GV, Venkatesh T, Bhadrachari S, Nibin Joy M.	Pharmaceuticals (Basel)	10.3390/ph17050548	2024
	Sphingosine kills Mycobacteria and suppresses mycobacterial lung infections.	Wu Y, Schnitker F, Liu Y, Keitsch S, Caicci F, Schumacher F, Riehle A, Pollmeier B, Kehrmann J, Kleuser B, Kamler M, Szabo I, Grassme H, Gulbins E.	J Mol Med (Berl)	10.1007/s00109-025-02534-z	2025
	Investigation of the antimycobacterial activity of African medicinal plants combined with chemometric analysis to identify potential leads.	Moyo P, Ofori M, Bodede OS, Wooding M, Khorommbi NK, McGaw LJ, Danquah CA, Maharaj VJ.	Sci Rep	10.1038/s41598-024-65369-7	2024
	Identification of a mycobacterial hydrazidase, an isoniazid-hydrolyzing enzyme.	Sakiyama A, Saren C, Kaneko Y, Oinuma KI.	Sci Rep	10.1038/s41598-023-35213-5	2023
Pathogenicity	Mycobacterium tuberculosis protein Rv2652c enhances intracellular survival by inhibiting host immune responses.	Li J, Dou Y.	Immun Inflamm Dis	10.1002/iid3.70012	2024
	A Novel Oxazolidinone, Contezolid (MRX-I), Expresses Anti-Mycobacterium abscessus Activity In Vitro.	Guo Q, Xu L, Tan F, Zhang Y, Fan J, Wang X, Zhang Z, Li B, Chu H.	Antimicrob Agents Chemother	10.1128/aac.00889-21	2021
	Structural and mechanistic analysis of ATPase inhibitors targeting mycobacterial DNA gyrase.	Henderson SR, Stevenson CEM, Malone B, Zholnerovych Y, Mitchenall LA, Pichowicz M, McGarry DH, Cooper IR, Charrier C, Salisbury AM, Lawson DM, Maxwell A.	J Antimicrob Chemother	10.1093/jac/dkaa286	2020
	Investigating the Antituberculosis Activity of Selected Commercial Essential Oils and Identification of Active Constituents Using a Biochemometrics Approach and In Silico Modeling.	Boussamba-Digombou KJ, Sandasi M, Kamatou GP, van Vuuren S, Sawicki R, Fakhar Z, Viljoen AM.	Antibiotics (Basel)	10.3390/antibiotics11070948	2022
Metabolism	Genome-guided and mass spectrometry investigation of natural products produced by a potential new actinobacterial strain isolated from a mangrove ecosystem in Futian, Shenzhen, China.	Hu D, Gao C, Sun C, Jin T, Fan G, Mok KM, Lee SM.	Sci Rep	10.1038/s41598-018-37475-w	2019
	Nigella damascena L. Essential Oil-A Valuable Source of beta-Elemene for Antimicrobial Testing.	Sieniawska E, Sawicki R, Golus J, Swatko-Ossor M, Ginalska G, Skalicka-Wozniak K.	Molecules	10.3390/molecules23020256	2018
	Human Neutrophil Granule Exocytosis in Response to Mycobacterium smegmatis.	Miralda I, Klaes CK, Graham JE, Uriarte SM.	Pathogens	10.3390/pathogens9020123	2020
	Development of 2nd generation aminomethyl spectinomycins that overcome native efflux in Mycobacterium abscessus.	Phelps GA, Cheramie MN, Fernando DM, Selchow P, Meyer CJ, Waidyarachchi SL, Dharuman S, Liu J, Meuli M, Molin MD, Killam BY, Murphy PA, Reeve SM, Wilt LA, Anderson SM, Yang L, Lee RB, Temrikar ZH, Lukka PB, Meibohm B, Polikanov YS, Hobbie SN, Bottger EC, Sander P, Lee RE.	Proc Natl Acad Sci U S A	10.1073/pnas.2314101120	2024
Pathogenicity	Untargeted metabolomics reveals a new mode of action of pretomanid (PA-824).	Baptista R, Fazakerley DM, Beckmann M, Baillie L, Mur LAJ.	Sci Rep	10.1038/s41598-018-23110-1	2018
Enzymology	Phylogenetic and Biochemical Analyses of Mycobacterial l,d-Transpeptidases Reveal a Distinct Enzyme Class That Is Preferentially Acylated by Meropenem.	Zandi TA, Marshburn RL, Stateler PK, Brammer Basta LA.	ACS Infect Dis	10.1021/acsinfecdis.9b00234	2019
Pathogenicity	In Vitro Susceptibility Testing of Bedaquiline against Mycobacterium abscessus Complex.	Brown-Elliott BA, Wallace RJ.	Antimicrob Agents Chemother	10.1128/aac.01919-18	2019
	Tea Tree Oil: Properties and the Therapeutic Approach to Acne-A Review.	Nascimento T, Gomes D, Simoes R, da Graca Miguel M.	Antioxidants (Basel)	10.3390/antiox12061264	2023
	In Vitro Activity of the Sudapyridine (WX-081) against Non-Tuberculous Mycobacteria Isolated in Beijing, China.	Zhu R, Shang Y, Chen S, Xiao H, Ren R, Wang F, Xue Y, Li L, Li Y, Chu N, Huang H.	Microbiol Spectr	10.1128/spectrum.01372-22	2022
Phylogeny	Gene and whole genome analyses reveal that the mycobacterial strain JS623 is not a member of the species Mycobacterium smegmatis.	Garcia MJ, Gola S.	Microb Biotechnol	10.1111/1751-7915.12336	2016
	Mycolicibacterium smegmatis, Basonym Mycobacterium smegmatis, Expresses Morphological Phenotypes Much More Similar to Escherichia coli Than Mycobacterium tuberculosis in Quantitative Structome Analysis and CryoTEM Examination.	Yamada H, Yamaguchi M, Igarashi Y, Chikamatsu K, Aono A, Murase Y, Morishige Y, Takaki A, Chibana H, Mitarai S.	Front Microbiol	10.3389/fmicb.2018.01992	2018
	Investigating Ghanaian Allium Species for Anti-Infective and Resistance-Reversal Natural Product Leads to Mitigate Multidrug-Resistance in Tuberculosis.	Danquah CA, Tetteh M, Amponsah IK, Mensah AY, Buabeng KO, Gibbons S, Bhakta S.	Antibiotics (Basel)	10.3390/antibiotics10080902	2021
	In Vitro and In Vivo Properties of CUO246, a Novel Bacterial DNA Gyrase/Topoisomerase IV Inhibitor.	Blais J, Dean CR, Lapointe G, Leeds JA, Ma S, Morris L, Moser HE, Osborne CS, Prosen KR, Richie D, Skepper C, Thompson K, Vo J, Yue Q, Rivkin A.	Antimicrob Agents Chemother	10.1128/aac.00921-22	2022
	In Vitro Antimicrobial Activities of Tigecycline, Eravacycline, Omadacycline, and Sarecycline against Rapidly Growing Mycobacteria.	Zhang T, Du J, Dong L, Wang F, Zhao L, Jia J, Wang C, Cheng M, Yu X, Huang H.	Microbiol Spectr	10.1128/spectrum.03238-22	2023
	Essential Oil Blends: The Potential of Combined Use for Respiratory Tract Infections.	Leigh-de Rapper S, Viljoen A, van Vuuren S.	Antibiotics (Basel)	10.3390/antibiotics10121517	2021
	In vitro and intracellular inhibitory activities of nosiheptide against Mycobacterium abscessus.	Zhu R, Yu X, Zhang T, Kong Y, Wang F, Jia J, Xue Y, Huang H.	Front Microbiol	10.3389/fmicb.2022.926361	2022
	Antimicrobial Activity and Mode of Action of Celastrol, a Nortriterpen Quinone Isolated from Natural Sources.	Padilla-Montano N, de Leon Guerra L, Moujir L.	Foods	10.3390/foods10030591	2021
	Identification of D-arabinan-degrading enzymes in mycobacteria.	Al-Jourani O, Benedict ST, Ross J, Layton AJ, van der Peet P, Marando VM, Bailey NP, Heunis T, Manion J, Mensitieri F, Franklin A, Abellon-Ruiz J, Oram SL, Parsons L, Cartmell A, Wright GSA, Basle A, Trost M, Henrissat B, Munoz-Munoz J, Hirt RP, Kiessling LL, Lovering AL, Williams SJ, Lowe EC, Moynihan PJ.	Nat Commun	10.1038/s41467-023-37839-5	2023
Pathogenicity	In Vitro Susceptibility Testing of Tedizolid against Nontuberculous Mycobacteria.	Brown-Elliott BA, Wallace RJ.	J Clin Microbiol	10.1128/jcm.00274-17	2017
Metabolism	Structural Basis of Mycobacterium tuberculosis Transcription and Transcription Inhibition.	Lin W, Mandal S, Degen D, Liu Y, Ebright YW, Li S, Feng Y, Zhang Y, Mandal S, Jiang Y, Liu S, Gigliotti M, Talaue M, Connell N, Das K, Arnold E, Ebright RH.	Mol Cell	10.1016/j.molcel.2017.03.001	2017
	Novel Naja atra cardiotoxin 1 (CTX-1) derived antimicrobial peptides with broad spectrum activity.	Sala A, Cabassi CS, Santospirito D, Polverini E, Flisi S, Cavirani S, Taddei S.	PLoS One	10.1371/journal.pone.0190778	2018
	Development and validation of technologies suitable for the decontamination and re-use of contaminated N95 filtering facepiece respirators in response to the COVID-19 pandemic.	Alt J, Eveland R, Fiorello A, Haas B, Meszaros J, McEvoy B, Ridenour C, Shaffer D, Yirava W, Ward L.	J Hosp Infect	10.1016/j.jhin.2021.10.004	2022
Pathogenicity	Magnesium corrosion particles do not interfere with the immune function of primary human and murine macrophages.	Roth I, Schumacher S, Basler T, Baumert K, Seitz JM, Evertz F, Muller PP, Baumer W, Kietzmann M.	Prog Biomater	10.1007/s40204-014-0032-9	2015
	Natural Products-Pyrazine Hybrids: A Review of Developments in Medicinal Chemistry.	Chen GQ, Guo HY, Quan ZS, Shen QK, Li X, Luan T.	Molecules	10.3390/molecules28217440	2023
Metabolism	Reconstitution of Protein Translation of Mycobacterium Reveals Functional Conservation and Divergence with the Gram-Negative Bacterium Escherichia coli.	Srivastava A, Asahara H, Zhang M, Zhang W, Liu H, Cui S, Jin Q, Chong S.	PLoS One	10.1371/journal.pone.0162020	2016
	Bioactive Constituents, Radical Scavenging, and Antibacterial Properties of the Leaves and Stem Essential Oils from Peperomia pellucida (L.) Kunth.	Okoh SO, Iweriebor BC, Okoh OO, Okoh AI.	Pharmacogn Mag	10.4103/pm.pm_106_17	2017
Phylogeny	Mycobacterium goodii endocarditis following mitral valve ring annuloplasty.	Parikh RB, Grant M.	Ann Clin Microbiol Antimicrob	10.1186/s12941-017-0190-4	2017
Pathogenicity	Characterization of Volatile Organic Compounds Emitted from Endophytic Burkholderia cenocepacia ETR-B22 by SPME-GC-MS and Their Inhibitory Activity against Various Plant Fungal Pathogens.	Chen JH, Xiang W, Cao KX, Lu X, Yao SC, Hung D, Huang RS, Li LB.	Molecules	10.3390/molecules25173765	2020
Phylogeny	Performance of Vitek MS v3.0 for Identification of Mycobacterium Species from Patient Samples by Use of Automated Liquid Medium Systems.	Miller E, Cantrell C, Beard M, Derylak A, Babady NE, McMillen T, Miranda E, Body B, Tang YW, Vasireddy R, Vasireddy S, Smith T, Iakhiaeva E, Wallace RJ, Brown-Elliott BA, Moreno E, Totty H, Deol P.	J Clin Microbiol	10.1128/jcm.00219-18	2018
Pathogenicity	Efficacy of carvacrol against resistant rapidly growing mycobacteria in the planktonic and biofilm growth mode.	Marini E, Di Giulio M, Ginestra G, Magi G, Di Lodovico S, Marino A, Facinelli B, Cellini L, Nostro A.	PLoS One	10.1371/journal.pone.0219038	2019
	Fundamental Cell Morphologies Examined With Cryo-TEM of the Species in the Novel Five Genera Robustly Correlate With New Classification in Family Mycobacteriaceae.	Yamada H, Chikamatsu K, Aono A, Murata K, Miyazaki N, Kayama Y, Bhatt A, Fujiwara N, Maeda S, Mitarai S.	Front Microbiol	10.3389/fmicb.2020.562395	2020
Pathogenicity	[Inactivation of mycobacteria by disinfectants with a tuberculocidal label].	Bello T, Rivera-Olivero IA, de Waard JH.	Enferm Infecc Microbiol Clin	10.1157/13089667	2006
Pathogenicity	In vitro activities of tigecycline and 10 other antimicrobials against nonpigmented rapidly growing mycobacteria.	Fernandez-Roblas R, Martin-de-Hijas NZ, Fernandez-Martinez AI, Garcia-Almeida D, Gadea I, Esteban J.	Antimicrob Agents Chemother	10.1128/aac.00695-08	2008
Phylogeny	Non-tuberculous Mycobacteria in South African Wildlife: Neglected Pathogens and Potential Impediments for Bovine Tuberculosis Diagnosis.	Gcebe N, Hlokwe TM.	Front Cell Infect Microbiol	10.3389/fcimb.2017.00015	2017
Pathogenicity	In Vitro Activities of Bedaquiline and Delamanid against Nontuberculous Mycobacteria Isolated in Beijing, China.	Yu X, Gao X, Li C, Luo J, Wen S, Zhang T, Ma Y, Dong L, Wang F, Huang H.	Antimicrob Agents Chemother	10.1128/aac.00031-19	2019
	Gran1: A Granulysin-Derived Peptide with Potent Activity against Intracellular Mycobacterium tuberculosis.	Noschka R, Wondany F, Kizilsavas G, Weil T, Weidinger G, Walther P, Michaelis J, Stenger S.	Int J Mol Sci	10.3390/ijms22168392	2021
	Identification of 2-Aryl-Quinolone Inhibitors of Cytochrome bd and Chemical Validation of Combination Strategies for Respiratory Inhibitors against Mycobacterium tuberculosis.	Jeffreys LN, Ardrey A, Hafiz TA, Dyer LA, Warman AJ, Mosallam N, Nixon GL, Fisher NE, Hong WD, Leung SC, Aljayyoussi G, Bibby J, Almeida DV, Converse PJ, Fotouhi N, Berry NG, Nuermberger EL, Upton AM, O'Neill PM, Ward SA, Biagini GA.	ACS Infect Dis	10.1021/acsinfecdis.2c00283	2023
	Therapeutic Uses and Pharmacological Properties of Shallot (Allium ascalonicum): A Systematic Review.	Moldovan C, Frumuzachi O, Babota M, Barros L, Mocan A, Carradori S, Crisan G.	Front Nutr	10.3389/fnut.2022.903686	2022
Metabolism	cGAS-STING-TBK1-IRF3/7 induced interferon-beta contributes to the clearing of non tuberculous mycobacterial infection in mice.	Ruangkiattikul N, Nerlich A, Abdissa K, Lienenklaus S, Suwandi A, Janze N, Laarmann K, Spanier J, Kalinke U, Weiss S, Goethe R.	Virulence	10.1080/21505594.2017.1321191	2017
	Bacterial diversity obtained by culturable approaches in the gut of Glossina pallidipes population from a non sleeping sickness focus in Tanzania: preliminary results.	Malele I, Nyingilili H, Lyaruu E, Tauzin M, Bernard Ollivier B, Cayol JL, Fardeau ML, Geiger A.	BMC Microbiol	10.1186/s12866-018-1288-3	2018
Pathogenicity	Broad-spectrum in vitro antibacterial activities of clay minerals against antibiotic-susceptible and antibiotic-resistant bacterial pathogens.	Haydel SE, Remenih CM, Williams LB.	J Antimicrob Chemother	10.1093/jac/dkm468	2008
	Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa.	Igwaran A, Iweriebor BC, Ofuzim Okoh S, Nwodo UU, Obi LC, Okoh AI.	BMC Complement Altern Med	10.1186/s12906-017-1861-6	2017
	Acid-fast staining and Petroff-Hausser chamber counting of mycobacterial cells in liquid suspension.	Treuer R, Haydel SE.	Curr Protoc Microbiol	10.1002/9780471729259.mc10a06s20	2011
Genetics	Comparative genomic hybridizations reveal genetic regions within the Mycobacterium avium complex that are divergent from Mycobacterium avium subsp. paratuberculosis isolates.	Paustian ML, Kapur V, Bannantine JP.	J Bacteriol	10.1128/jb.187.7.2406-2415.2005	2005
Enzymology	Development of a novel PCR restriction analysis of the hsp65 gene as a rapid method to screen for the Mycobacterium tuberculosis complex and nontuberculous mycobacteria in high-burden countries.	Varma-Basil M, Garima K, Pathak R, Dwivedi SK, Narang A, Bhatnagar A, Bose M.	J Clin Microbiol	10.1128/jcm.03067-12	2013
Pathogenicity	In vitro antimycobacterial activity of 5-chloropyrazinamide.	Cynamon MH, Speirs RJ, Welch JT.	Antimicrob Agents Chemother	10.1128/aac.42.2.462	1998
	Biofilm development by potentially pathogenic non-pigmented rapidly growing mycobacteria.	Esteban J, Martin-de-Hijas NZ, Kinnari TJ, Ayala G, Fernandez-Roblas R, Gadea I.	BMC Microbiol	10.1186/1471-2180-8-184	2008
Metabolism	Mycobacterium tuberculosis Is a Natural Ornithine Aminotransferase (rocD) Mutant and Depends on Rv2323c for Growth on Arginine.	Hampel A, Huber C, Geffers R, Spona-Friedl M, Eisenreich W, Bange FC.	PLoS One	10.1371/journal.pone.0136914	2015
Pathogenicity	Structure-activity relationships of quinolone agents against mycobacteria: effect of structural modifications at the 8 position.	Renau TE, Gage JW, Dever JA, Roland GE, Joannides ET, Shapiro MA, Sanchez JP, Gracheck SJ, Domagala JM, Jacobs MR, Reynolds RC.	Antimicrob Agents Chemother	10.1128/aac.40.10.2363	1996
Enzymology	Evaluation of methods for the extraction of DNA from drinking water distribution system biofilms.	Hwang C, Ling F, Andersen GL, LeChevallier MW, Liu WT.	Microbes Environ	10.1264/jsme2.me11132	2012
Phylogeny	Biochip system for rapid and accurate identification of mycobacterial species from isolates and sputum.	Zhu L, Jiang G, Wang S, Wang C, Li Q, Yu H, Zhou Y, Zhao B, Huang H, Xing W, Mitchelson K, Cheng J, Zhao Y, Guo Y.	J Clin Microbiol	10.1128/jcm.00158-10	2010
Enzymology	High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis.	Butler WR, Kilburn JO.	J Clin Microbiol	10.1128/jcm.28.9.2094-2098.1990	1990
Pathogenicity	Antibiotic susceptibility pattern of Mycobacterium marinum.	Aubry A, Jarlier V, Escolano S, Truffot-Pernot C, Cambau E.	Antimicrob Agents Chemother	10.1128/aac.44.11.3133-3136.2000	2000
Phylogeny	Characterization of a trinucleotide repeat sequence (CGG)5 and potential use in restriction fragment length polymorphism typing of Mycobacterium tuberculosis.	Otsuka Y, Parniewski P, Zwolska Z, Kai M, Fujino T, Kirikae F, Toyota E, Kudo K, Kuratsuji T, Kirikae T.	J Clin Microbiol	10.1128/jcm.42.8.3538-3548.2004	2004
Metabolism	The glycan-rich outer layer of the cell wall of Mycobacterium tuberculosis acts as an antiphagocytic capsule limiting the association of the bacterium with macrophages.	Stokes RW, Norris-Jones R, Brooks DE, Beveridge TJ, Doxsee D, Thorson LM.	Infect Immun	10.1128/iai.72.10.5676-5686.2004	2004
	Nonopsonic binding of Mycobacterium tuberculosis to complement receptor type 3 is mediated by capsular polysaccharides and is strain dependent.	Cywes C, Hoppe HC, Daffe M, Ehlers MR.	Infect Immun	10.1128/iai.65.10.4258-4266.1997	1997
	Identification of phosphatidylinositol mannoside as a mycobacterial adhesin mediating both direct and opsonic binding to nonphagocytic mammalian cells.	Hoppe HC, de Wet BJ, Cywes C, Daffe M, Ehlers MR.	Infect Immun	10.1128/iai.65.9.3896-3905.1997	1997
Enzymology	Group-specific small-subunit rRNA hybridization probes to characterize filamentous foaming in activated sludge systems.	de los Reyes FL, Ritter W, Raskin L.	Appl Environ Microbiol	10.1128/aem.63.3.1107-1117.1997	1997
Phylogeny	Multiprimer PCR system for differential identification of mycobacteria in clinical samples.	Del Portillo P, Thomas MC, Martinez E, Maranon C, Valladares B, Patarroyo ME, Carlos Lopez M.	J Clin Microbiol	10.1128/jcm.34.2.324-328.1996	1996
	Genotypic variation and stability of four variable-number tandem repeats and their suitability for discriminating strains of Mycobacterium leprae.	Truman R, Fontes AB, De Miranda AB, Suffys P, Gillis T.	J Clin Microbiol	10.1128/jcm.42.6.2558-2565.2004	2004
Metabolism	Nonopsonic binding of Mycobacterium tuberculosis to human complement receptor type 3 expressed in Chinese hamster ovary cells.	Cywes C, Godenir NL, Hoppe HC, Scholle RR, Steyn LM, Kirsch RE, Ehlers MR.	Infect Immun	10.1128/iai.64.12.5373-5383.1996	1996
Pathogenicity	Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. The Tuberculosis Working Group in Peru.	Caviedes L, Lee TS, Gilman RH, Sheen P, Spellman E, Lee EH, Berg DE, Montenegro-James S.	J Clin Microbiol	10.1128/jcm.38.3.1203-1208.2000	2000
	Macrophage activation by bacterial cell walls and related synthetic compounds.	Takada H, Tsujimoto M, Kato K, Kotani S, Kusumoto S, Inage M, Shiba T, Yano I, Kawata S, Yokogawa K.	Infect Immun	10.1128/iai.25.1.48-53.1979	1979
	Serological expression cloning and immunological evaluation of MTB48, a novel Mycobacterium tuberculosis antigen.	Lodes MJ, Dillon DC, Mohamath R, Day CH, Benson DR, Reynolds LD, McNeill P, Sampaio DP, Skeiky YA, Badaro R, Persing DH, Reed SG, Houghton RL.	J Clin Microbiol	10.1128/jcm.39.7.2485-2493.2001	2001
	Cloning, expression, and immunological evaluation of two putative secreted serine protease antigens of Mycobacterium tuberculosis.	Skeiky YA, Lodes MJ, Guderian JA, Mohamath R, Bement T, Alderson MR, Reed SG.	Infect Immun	10.1128/iai.67.8.3998-4007.1999	1999
	Serological response of patients with leprosy to a 28- to 30-kilodalton protein doublet from early cultures of Mycobacterium bovis BCG.	Pessolani MC, Rumjanek FD, Marques MA, de Melo FS, Sarno EN.	J Clin Microbiol	10.1128/jcm.27.10.2184-2189.1989	1989
	Evolution and expansion of the Mycobacterium tuberculosis PE and PPE multigene families and their association with the duplication of the ESAT-6 (esx) gene cluster regions.	Gey van Pittius NC, Sampson SL, Lee H, Kim Y, van Helden PD, Warren RM.	BMC Evol Biol	10.1186/1471-2148-6-95	2006
	Evaluation of EZplex MTBC/NTM Real-Time PCR kit: diagnostic accuracy and efficacy in vaccination.	Lee S, Hwang KA, Ahn JH, Nam JH.	Clin Exp Vaccine Res	10.7774/cevr.2018.7.2.111	2018
	A novel class of antimicrobial drugs selectively targets a Mycobacterium tuberculosis PE-PGRS protein.	Seo H, Kim S, Mahmud HA, Islam MI, Yoon Y, Cho HD, Nam KW, Choi J, Gil YS, Lee BE, Song HY.	PLoS Biol	10.1371/journal.pbio.3001648	2022
	Enrichment of LDPE-degrading bacterial consortia: Community succession and enhanced degradation efficiency through various pretreatment methods.	Muangchinda C, Pinyakong O.	Sci Rep	10.1038/s41598-024-80306-4	2024
	Application of Monoclonal Anti-Mycolate Antibodies in Serological Diagnosis of Tuberculosis.	Truyts A, Du Preez I, Maesela EM, Scriba MR, Baillie L, Jones AT, Land KJ, Verschoor JA, Lemmer Y.	Trop Med Infect Dis	10.3390/tropicalmed9110269	2024
Metabolism	Carbamate C-N Hydrolase Gene ameH Responsible for the Detoxification Step of Methomyl Degradation in Aminobacter aminovorans Strain MDW-2.	Jiang W, Zhang C, Gao Q, Zhang M, Qiu J, Yan X, Hong Q.	Appl Environ Microbiol	10.1128/aem.02005-20	2020
Enzymology	Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform.	Baron VO, Chen M, Hammarstrom B, Hammond RJH, Glynne-Jones P, Gillespie SH, Dholakia K.	Commun Biol	10.1038/s42003-020-0915-3	2020
Enzymology	Characterisation of methionine adenosyltransferase from Mycobacterium smegmatis and M. tuberculosis.	Berger BJ, Knodel MH.	BMC Microbiol	10.1186/1471-2180-3-12	2003
	Improved green fluorescent protein reporter gene-based microplate screening for antituberculosis compounds by utilizing an acetamidase promoter.	Changsen C, Franzblau SG, Palittapongarnpim P.	Antimicrob Agents Chemother	10.1128/aac.47.12.3682-3687.2003	2003
	Characterization of an rRNA operon (rrnB) of Mycobacterium fortuitum and other mycobacterial species: implications for the classification of mycobacteria.	Menendez MC, Garcia MJ, Navarro MC, Gonzalez-y-Merchand JA, Rivera-Gutierrez S, Garcia-Sanchez L, Cox RA.	J Bacteriol	10.1128/jb.184.4.1078-1088.2002	2002
	Effects of a substituted diphenylthiourea (Ciba 1906) on Mycobacterium smegmatis NCTC 8159.	Draper P.	Tubercle	10.1016/s0041-3879(67)80011-4	1967
Enzymology	Cloning and heterologous expression of an enantioselective amidase from Rhodococcus erythropolis strain MP50.	Trott S, Burger S, Calaminus C, Stolz A.	Appl Environ Microbiol	10.1128/aem.68.7.3279-3286.2002	2002
	Roles of multiple promoters in transcription of ribosomal DNA: effects of growth conditions on precursor rRNA synthesis in mycobacteria.	Gonzalez-y-Merchand JA, Colston MJ, Cox RA.	J Bacteriol	10.1128/jb.180.21.5756-5761.1998	1998
	Effects of growth conditions on expression of mycobacterial murA and tyrS genes and contributions of their transcripts to precursor rRNA synthesis.	Gonzalez-y-Merchand JA, Colston MJ, Cox RA.	J Bacteriol	10.1128/jb.181.15.4617-4627.1999	1999
Phylogeny	Identification of Brevibacterium from clinical sources.	Pitcher DG, Malnick H.	J Clin Pathol	10.1136/jcp.37.12.1395	1984
Enzymology	L-selective amidase with extremely broad substrate specificity from Ochrobactrum anthropi NCIMB 40321.	Sonke T, Ernste S, Tandler RF, Kaptein B, Peeters WP, van Assema FB, Wubbolts MG, Schoemaker HE.	Appl Environ Microbiol	10.1128/aem.71.12.7961-7973.2005	2005
Metabolism	Strategies used by pathogenic and nonpathogenic mycobacteria to synthesize rRNA.	Gonzalez-y-Merchand JA, Garcia MJ, Gonzalez-Rico S, Colston MJ, Cox RA.	J Bacteriol	10.1128/jb.179.22.6949-6958.1997	1997
Pathogenicity	PHACOS, a functionalized bacterial polyester with bactericidal activity against methicillin-resistant Staphylococcus aureus.	Dinjaski N, Fernandez-Gutierrez M, Selvam S, Parra-Ruiz FJ, Lehman SM, San Roman J, Garcia E, Garcia JL, Garcia AJ, Prieto MA.	Biomaterials	10.1016/j.biomaterials.2013.09.059	2014
	Genomics Insights into Mycolicibacterium Hassiacum Causing Infection in a Cat with Pyogranulomatous Dermatitis and Panniculitis.	Smedile D, Iurescia M, Carfora V, Cocumelli C, Palmerini T, Diaconu EL, Congiu I, Donati V, Stravino F, Sorbara L, Romano E, Caprioli A, Battisti A.	Pathogens	10.3390/pathogens13090785	2024
Genetics	Discovery of Antimicrobial Phosphonopeptide Natural Products from Bacillus velezensis by Genome Mining.	Wilson J, Cui J, Nakao T, Kwok H, Zhang Y, Kayrouz CM, Pham TM, Roodhouse H, Ju KS.	Appl Environ Microbiol	10.1128/aem.00338-23	2023
	Ent-homocyclopiamine B, a Prenylated Indole Alkaloid of Biogenetic Interest from the Endophytic Fungus Penicillium concentricum.	Ali T, Pham TM, Ju KS, Rakotondraibe HL.	Molecules	10.3390/molecules24020218	2019
Metabolism	A new genome-mining tool redefines the lasso peptide biosynthetic landscape.	Tietz JI, Schwalen CJ, Patel PS, Maxson T, Blair PM, Tai HC, Zakai UI, Mitchell DA.	Nat Chem Biol	10.1038/nchembio.2319	2017
Enzymology	Crystal structure and biochemical characterization of O-acetylhomoserine acetyltransferase from Mycobacterium smegmatis ATCC 19420.	Sagong HY, Hong J, Kim KJ	Biochem Biophys Res Commun	10.1016/j.bbrc.2019.07.117	2019
Pathogenicity	New quinolin-4-yl-1,2,3-triazoles carrying amides, sulphonamides and amidopiperazines as potential antitubercular agents.	Thomas KD, Adhikari AV, Chowdhury IH, Sumesh E, Pal NK	Eur J Med Chem	10.1016/j.ejmech.2011.03.039	2011
Genetics	A rifampin-hypersensitive mutant reveals differences between strains of Mycobacterium smegmatis and presence of a novel transposon, IS1623.	Alexander DC, Jones JR, Liu J	Antimicrob Agents Chemother	10.1128/AAC.47.10.3208-3213.2003	2003
Enzymology	Panniculitis, due to Mycobacterium smegmatis, in two Finnish cats.	Alander-Damsten YK, Brander EE, Paulin LG	J Feline Med Surg	10.1053/jfms.2001.0189	2003
Pathogenicity	Characterization of In53, a class 1 plasmid- and composite transposon-located integron of Escherichia coli which carries an unusual array of gene cassettes.	Naas T, Mikami Y, Imai T, Poirel L, Nordmann P	J Bacteriol	10.1128/JB.183.1.235-249.2001	2001
Metabolism	Structures of ADP-ribosylated rifampicin and its metabolite: intermediates of rifampicin-ribosylation by Mycobacterium smegmatis DSM43756.	Morisaki N, Hashimoto Y, Furihata K, Imai T, Watanabe K, Mikami Y, Yazawa K, Ando A, Nagata Y, Dabbs ER	J Antibiot (Tokyo)	10.7164/antibiotics.53.269	2000
Metabolism	Identification and characterization of a new intermediate in the ribosylative inactivation pathway of rifampin by Mycobacterium smegmatis.	Imai T, Watanabe K, Mikami Y, Yazawa K, Ando A, Nagata Y, Morisaki N, Hashimoto Y, Furihata K, Dabbs ER	Microb Drug Resist	10.1089/mdr.1999.5.259	1999
Enzymology	Pathogenicity of Mycobacterium fortuitum and Mycobacterium smegmatis to goldfish, Carassius auratus.	Talaat AM, Trucksis M, Kane AS, Reimschuessel R	Vet Microbiol	10.1016/s0378-1135(99)00002-4	1999
Metabolism	ADP-ribosylation as an intermediate step in inactivation of rifampin by a mycobacterial gene.	Quan S, Imai T, Mikami Y, Yazawa K, Dabbs ER, Morisaki N, Iwasaki S, Hashimoto Y, Furihata K	Antimicrob Agents Chemother	10.1128/AAC.43.1.181	1999
Genetics	Regulation of the inducible acetamidase gene of Mycobacterium smegmatis.	Parish T, Mahenthiralingam E, Draper P, Davis EO, Colston EO	Microbiology (Reading)	10.1099/00221287-143-7-2267	1997
Metabolism	Structure determination of ribosylated rifampicin and its derivative: new inactivated metabolites of rifampicin by mycobacterial strains.	Morisaki N, Kobayashi H, Iwasaki S, Furihata K, Dabbs ER, Yazawa K, Mikami Y	J Antibiot (Tokyo)	10.7164/antibiotics.48.1299	1995
Enzymology	Cloning and sequencing of the gene which encodes the highly inducible acetamidase of Mycobacterium smegmatis.	Mahenthiralingam E, Draper P, Davis EO, Colston MJ	J Gen Microbiol	10.1099/00221287-139-3-575	1993
Pathogenicity	Acanthamoeba polyphaga-enhanced growth of Mycobacterium smegmatis.	Lamrabet O, Mba Medie F, Drancourt M	PLoS One	10.1371/journal.pone.0029833	2012
Phylogeny	Mycobacterium appelbergii sp. nov., a Novel Species Isolated from a Drinking Water Fountain in a Rural Community.	Roxo IC, Alarico S, Fonseca A, Machado D, Maranha A, Tiago I, Duarte R, Empadinhas N.	Microorganisms	10.3390/microorganisms13061259	2025
Phylogeny	Mycobacterium wolinskyi sp. nov. and Mycobacterium goodii sp. nov., two new rapidly growing species related to Mycobacterium smegmatis and associated with human wound infections: a cooperative study from the International Working Group on Mycobacterial Taxonomy.	Brown BA, Springer B, Steingrube VA, Wilson RW, Pfyffer GE, Garcia MJ, Menendez MC, Rodriguez-Salgado B, Jost KC Jr, Chiu SH, Onyi GO, Bottger EC, Wallace RJ Jr	Int J Syst Bacteriol	10.1099/00207713-49-4-1493	1999

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#68382	Automatically annotated from API zym .
#122643	Collection of Institut Pasteur ; Curators of the CIP; CIP 104444
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mycobacterium smegmatis (DSM 43756, ATCC 19420, JCM 5866)

Name and taxonomic classification

Morphology

Cell morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001457595

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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