Name: Leuconostoc lactis CCUG 30064
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6818

Type strain:

Species: Leuconostoc lactis

Culture col. no.: CCUG 30064, NRRL B-3468, DSM 20202, ATCC 19256, NCDO 533, JCM 6123, BCRC 12261, CCM 4545, CECT 4173, CGMCC 1.2137, CIP 102422, HAMBI 2346, KCTC 3528, LMG 8894, NBRC 107866, NCIMB 13091, NRIC 1540, VTT E-98974

Strain history: CIP <- 1986, DSM <- NCDO <- E.I. Garvie, Reading, UK <- 1948, T. Gibson, Edinburgh, UK: strain L5, Streptococcus kefir

NCBI tax ID(s): 935294 (strain), 1246 (species)

SI-ID 3494

LMG 8894, ATCC 19256, CCUG 30064, DSM 20202, NCFB 533, NRRL B-3468, NCDO 533, CIP 102422, JCM 6123, NCIMB 13091, CCM4545, CECT 4173, CCRC 12261, KCTC 3528, AS 1.2137, BCRC 12261, CCT 0581, HAMBI 2346, CGMCC 1.2137, CGMCC 1.2451, VTT E-98974

Special Collections

Other strains from Leuconostoc lactis

Section

Leuconostoc lactis CCUG 30064 is an anaerobe, Gram-positive human pathogen that was isolated from milk.

Gram-positive
anaerobe
human pathogen
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Leuconostoc*
	Species	**Leuconostoc lactis**
	Full Scientific Name (LPSN)	Leuconostoc lactis Garvie 1960 (Approved Lists 1980)

Synonym

Leuconostoc argentinum

Information on morphological and physiological properties Morphology

[Ref.: #120529]	Gram stain	positive

[Ref.: #120529]	Cell shape	oval-shaped

[Ref.: #120529]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %92.5

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8473]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #8473]

Culture medium growth

[Ref.: #8473]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8473]

Culture medium composition

expand / minimize

[Ref.: #40418]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #40418]

Culture medium growth

[Ref.: #40418]

Culture medium composition

expand / minimize

[Ref.: #120529]

Culture medium

CIP Medium 40

[Ref.: #120529]

Culture medium growth

[Ref.: #120529]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #8473]		growth	30 °C
[Ref.: #40418]		growth	37 °C
[Ref.: #49883]		growth	37 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #120529]		growth	22-37 °C
[Ref.: #120529]	no	growth	10 °C
[Ref.: #120529]	no	growth	15 °C
[Ref.: #120529]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #49883]	Oxygen tolerance	anaerobe
[Ref.: #49883]	Oxygen tolerance	microaerophile
[Ref.: #120529]	Oxygen tolerance	facultative anaerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

[Ref.: #8473]	Murein short key	A11.05
[Ref.: #8473]	Murein types	A3alpha L-Lys-L-Ala₂

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #120529]	nitrate ChEBI	-	reduction
[Ref.: #120529]	nitrate ChEBI	+	respiration
[Ref.: #120529]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120529]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #120529]		acetoin ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120529]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120529]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120529]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120529]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120529]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120529]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #8473]	14559	-	-	-	-	-	-	-	-	-	-	+/-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	+	+	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #120529]	8966	not determinedn.d.	-	-	-	+	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120529]	7065	-	+	-	-	-	-	-	-	-	-	+	+	-	+	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8473]	Sample type/isolated from	milk

[Ref.: #67770]	Sample type/isolated from	Milk

[Ref.: #120529]	Sample type/isolated from	Food, Milk
* marker position based on {}

Isolation sources categories

#Host Body Product	#Fluids	#Milk
#Engineered	#Food production	#Dairy product

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC071838 (>99% sequence identity) for Leuconostoc from Microbeatlas

Aquatic Samples	3705
Soil Samples	2149
Animal Samples	23973
Plant Samples	1253
Total Samples	31080

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8473]	Pathogenicity (human)	yes, in single cases
[Ref.: #8473]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120529]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Leuconostoc lactis gene for 16S ribosomal RNA, partial sequence, strain: JCM 6123	LC071838	1475	GenBank ENA	935294 tax ID
[Ref.: #20218]	Leuconostoc lactis gene for 16S ribosomal RNA, partial sequence	D31668	199	GenBank ENA	935294 tax ID	*
[Ref.: #20218]	Leuconostoc lactis DNA, 16S-23S rRNA intergenic spacer region, strain: NRIC 1540	AB290441	372	GenBank ENA	935294 tax ID	*
[Ref.: #20218]	L.lactis rrn operon, 16S-23S rRNA spacer, tRNA-Ala gene	Z75492	375	GenBank ENA	935294 tax ID	*
[Ref.: #20218]	Leuconostoc lactis gene for 16S ribosomal RNA	AB023968	1451	GenBank ENA	935294 tax ID	*
[Ref.: #20218]	Leuconostoc lactis gene for 16S rRNA, partial sequence, strain: JCM 6123	AB596941	1472	GenBank ENA	935294 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Leuconostoc lactis KCTC 3528 = DSM 20202 JCM 6123	GCA_014651235	contig	GenBank ENA	935294 tax ID	*
[Ref.: #66792]	Leuconostoc lactis KCTC 3528 = DSM 20202 JCM 6123	GCA_019656035	contig	GenBank ENA	935294 tax ID	*
[Ref.: #66792]	Leuconostoc lactis KCTC 3528 = DSM 20202	GCA_000185085	contig	GenBank ENA	935294 tax ID	*
[Ref.: #66792]	Leuconostoc lactis KCTC 3528 = DSM 20202	935294.3	wgs	PATRIC	935294 tax ID	*
[Ref.: #66792]	Leuconostoc lactis KCTC 3528 = DSM 20202 strain JCM 6123	935294.7	wgs	PATRIC	935294 tax ID	*
[Ref.: #66792]	Leuconostoc lactis KCTC 3528 = DSM 20202 strain JCM 6123	935294.6	wgs	PATRIC	935294 tax ID	*

[Ref.: #8473]	GC-content	43.4 mol%
[Ref.: #67770]	GC-content	45 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Leuconostoc lactis KCTC 3528 = DSM 20202 strain JCM 6123 PATRIC: 935294.6
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Leuconostoc lactis KCTC 3528 = DSM 20202 PATRIC: 935294.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	89.556	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	82.823	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	93.564	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	89.642	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96.125	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	92.5	no

Availability in culture collections External links

[Ref.: #8473]

Culture collection no.

CCUG 30064, NRRL B-3468, DSM 20202, ATCC 19256, NCDO 533, JCM 6123, BCRC 12261, CCM 4545, CECT 4173, CGMCC 1.2137, CIP 102422, HAMBI 2346, KCTC 3528, LMG 8894, NBRC 107866, NCIMB 13091, NRIC 1540, VTT E-98974

[Ref.: #76284]

SI-ID 3494

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Leuconostoc palmae sp. nov., a novel lactic acid bacterium isolated from palm wine.	Ehrmann MA, Freiding S, Vogel RF	Int J Syst Evol Microbiol	10.1099/ijs.0.005983-0	2009	*
Enzymology	Characterization of Leuconostoc lactis strains from human sources.	Barreau C, Wagener G	J Clin Microbiol	10.1128/jcm.28.8.1728-1733.1990	1990	*
Genetics	Genome sequence analysis of potential probiotic strain Leuconostoc lactis EFEL005 isolated from kimchi.	Moon JS, Choi HS, Shin SY, Noh SJ, Jeon CO, Han NS	J Microbiol	10.1007/s12275-015-5090-8	2015	*

References

#8473

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20202

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40418 ; Curators of the CIP;
#49883 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30064

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76284

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID3494.1 )

#120529 Collection of Institut Pasteur ; Curators of the CIP; CIP 102422
* These data were automatically processed and therefore are not curated

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Leuconostoc lactis KCTC 3528 = DSM 20202 strain JCM 6123

Leuconostoc lactis KCTC 3528 = DSM 20202

Change proposal

Change proposal