Strain identifier

BacDive ID: 6818

Type strain: Yes

Species: Leuconostoc lactis

Strain history: CIP <- 1986, DSM <- NCDO <- E.I. Garvie, Reading, UK <- 1948, T. Gibson, Edinburgh, UK: strain L5, Streptococcus kefir

NCBI tax ID(s): 935294 (strain), 1246 (species)

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General

@ref: 8473

BacDive-ID: 6818

DSM-Number: 20202

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, Gram-positive, human pathogen

description: Leuconostoc lactis CCUG 30064 is an anaerobe, Gram-positive human pathogen that was isolated from milk.

NCBI tax id

NCBI tax idMatching level
1246species
935294strain

strain history

@refhistory
8473<- NCDO <- E.I. Garvie <- T. Gibson
67770ATCC 19256 <-- NCDO 533 <-- E. Garvie <-- T. Gibson (L5).
120529CIP <- 1986, DSM <- NCDO <- E.I. Garvie, Reading, UK <- 1948, T. Gibson, Edinburgh, UK: strain L5, Streptococcus kefir

doi: 10.13145/bacdive6818.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Leuconostoc
  • species: Leuconostoc lactis
  • full scientific name: Leuconostoc lactis Garvie 1960 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Leuconostoc argentinum

@ref: 8473

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Leuconostoc

species: Leuconostoc lactis

full scientific name: Leuconostoc lactis Garvie 1960

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
120529positiveoval-shapedno
69480no92.5

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8473MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
40418MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
120529CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
8473positivegrowth30
40418positivegrowth37
49883positivegrowth37
67770positivegrowth30
120529positivegrowth22-37
120529nogrowth10
120529nogrowth15
120529nogrowth45

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
49883anaerobe
49883microaerophile
120529facultative anaerobe

spore formation

  • @ref: 69481
  • spore formation: no
  • confidence: 100

murein

  • @ref: 8473
  • murein short key: A11.05
  • type: A3alpha L-Lys-L-Ala2

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120529nitrate-reduction17632
120529nitrite-reduction16301
120529nitrate+respiration17632

metabolite production

  • @ref: 120529
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 120529
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
120529oxidase-
120529alcohol dehydrogenase-1.1.1.1
120529catalase-1.11.1.6
120529lysine decarboxylase-4.1.1.18
120529ornithine decarboxylase-4.1.1.17
120529urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120529-+--------++-+------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8473----------+/-+-------------+/---++-+/-------------------
120529---+-----++----------------++-+------------------

Isolation, sampling and environmental information

isolation

@refsample type
8473milk
67770Milk
120529Food, Milk

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Fluids#Milk
#Engineered#Food production#Dairy product

taxonmaps

  • @ref: 69479
  • File name: preview.99_2892.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_22;96_35;97_348;98_2237;99_2892&stattab=map
  • Last taxonomy: Leuconostoc
  • 16S sequence: LC071838
  • Sequence Identity:
  • Total samples: 31080
  • soil counts: 2149
  • aquatic counts: 3705
  • animal counts: 23973
  • plant counts: 1253

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
8473yes, in single cases1Risk group (German classification)
1205291Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218L.lactis rrn operon, 16S-23S rRNA spacer, tRNA-Ala geneZ75492375nuccore935294
20218Leuconostoc lactis gene for 16S ribosomal RNAAB0239681451nuccore935294
20218Leuconostoc lactis gene for 16S rRNA, partial sequence, strain: JCM 6123AB5969411472nuccore935294
20218Leuconostoc lactis gene for 16S ribosomal RNA, partial sequenceD31668199nuccore935294
20218Leuconostoc lactis DNA, 16S-23S rRNA intergenic spacer region, strain: NRIC 1540AB290441372nuccore935294
67770Leuconostoc lactis gene for 16S ribosomal RNA, partial sequence, strain: JCM 6123LC0718381475nuccore935294

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Leuconostoc lactis KCTC 3528 = DSM 20202 JCM 6123GCA_014651235contigncbi935294
66792Leuconostoc lactis KCTC 3528 = DSM 20202 JCM 6123GCA_019656035contigncbi935294
66792Leuconostoc lactis KCTC 3528 = DSM 20202GCA_000185085contigncbi935294
66792Leuconostoc lactis KCTC 3528 = DSM 20202935294.3wgspatric935294
66792Leuconostoc lactis KCTC 3528 = DSM 20202 strain JCM 6123935294.7wgspatric935294
66792Leuconostoc lactis KCTC 3528 = DSM 20202 strain JCM 6123935294.6wgspatric935294
66792Leuconostoc lactis Gibson L5, KCTC 35282547132286draftimg935294

GC content

@refGC-contentmethod
847343.4
6777045thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes89.556no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no82.823no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no93.564yes
69480spore-formingspore-formingAbility to form endo- or exosporesno89.642no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno96.125yes
69480flagellatedmotile2+Ability to perform flagellated movementno92.5no

External links

@ref: 8473

culture collection no.: CCUG 30064, NRRL B-3468, DSM 20202, ATCC 19256, NCDO 533, JCM 6123, BCRC 12261, CCM 4545, CECT 4173, CGMCC 1.2137, CIP 102422, HAMBI 2346, KCTC 3528, LMG 8894, NBRC 107866, NCIMB 13091, NRIC 1540, VTT E-98974

straininfo link

  • @ref: 76284
  • straininfo: 3494

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology2394800Characterization of Leuconostoc lactis strains from human sources.Barreau C, Wagener GJ Clin Microbiol10.1128/jcm.28.8.1728-1733.19901990Amino Acids/analysis, Carbohydrates/analysis, Electrophoresis, Polyacrylamide Gel, Glycolipids/analysis, Humans, Infant, Newborn, Leuconostoc/*classification/growth & development, Peptidoglycan/analysisPathogenicity
Phylogeny19406772Leuconostoc palmae sp. nov., a novel lactic acid bacterium isolated from palm wine.Ehrmann MA, Freiding S, Vogel RFInt J Syst Evol Microbiol10.1099/ijs.0.005983-02009*Arecaceae, Bacterial Proteins/genetics, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fermentation, Genes, rRNA, Lactic Acid/metabolism, Leuconostoc/*classification/genetics/isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Senegal, Sequence Analysis, DNA, Species Specificity, Wine/*microbiologyGenetics
Genetics25935305Genome sequence analysis of potential probiotic strain Leuconostoc lactis EFEL005 isolated from kimchi.Moon JS, Choi HS, Shin SY, Noh SJ, Jeon CO, Han NSJ Microbiol10.1007/s12275-015-5090-82015Amino Acid Transport Systems/genetics, Bacteriocins/genetics, Base Composition, Base Sequence, Carbohydrate Metabolism/genetics, Carboxy-Lyases/genetics, Cation Transport Proteins/genetics, Fermentation, *Food Microbiology, *Genome, Bacterial, Leuconostoc/*genetics/*isolation & purification, Phylogeny, *Probiotics, *Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8473Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20202)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20202
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40418Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14107
49883Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30064)https://www.ccug.se/strain?id=30064
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76284Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3494.1StrainInfo: A central database for resolving microbial strain identifiers
120529Curators of the CIPCollection of Institut Pasteur (CIP 102422)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102422