[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Stock solution 1 (in 100ml): (NH4)2SO4 0.5g K2HPO4 7.5g
Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g
Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g
Basal medium: Agar 1.5%
Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g
Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g
Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g
Basal medium: Agar 1.5%
Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5%
Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml
Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Casitone(Difco) 0.3% yeast extract 0.1% CaCl2 0.1% Agar 1.5%
Preparation / Remarks: pH 7.2 (KOH) autoclaved *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 %
Preparation / Remarks: pH 7.2 autoclaved Combine with 1 mg/L Kanamycin-sulfate (sterilized by filtration) *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamine B12 0.5 µg/ml
Preparation / Remarks: pH 7.2 autoclaved Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416. *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Probion L 600 (Hoechst) 0.8% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% Agar 1.5%
Preparation / Remarks: pH 7.2 autoclaved *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml
Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on cy-plates. *
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[Ref.: #18281] |
Culture medium |
STAN 21 AGAR with Filter * | |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: 1. Agar 1% K2HPO4 0.1% Yeast extract (Difco) 0.002%
2. (NH4)2SO4 0.1% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% MnSO4 x H2O 0.01% FeCl3 x 6H2O 0.02% MnSO4 x 7H2O 0.01% FeCl3 0.02% Traceelement-solution 1ml to 100 ml
Preparation / Remarks: 1. in 2/3 of the volume after autoclaving 1 + 2 together and pour plates. Hang up a sterile-filter paper *
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[Ref.: #18281] |
Culture medium |
STAN 46 AGAR with Filter * | |
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[Ref.: #18281] |
Culture medium growth |
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[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: 1. Agar 1% K2HPO4 0.1% Yeast extract (Difco) 0.002% HEPES 25 mM
2. (NH4)2SO4 0.1% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% MnSO4 x H2O 0.01% FeCl3 x 6H2O 0.02% MnSO4 x 7H2O 0.01% FeCl3 0.02% Traceelement-solution 1ml to 100 ml
Preparation / Remarks: 1. in 2/3 of the volume after autoclaving 1 + 2 together and pour plates. Hang up a sterile-filter paper *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: CaCl2 x 2 H2O1.3g Agar (Difco)15.0g Distilled water1000.0ml
Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak autoclaved E.coli cells *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 %
Preparation / Remarks: pH 7.2 autoclaved *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Xylan (oat hulls 95%) 0.5% (NH4)2SO4 0.1% Agar 1%
Preparation / Remarks: pH 7.2 Xylan (Serva pure molecular weight 25000) Pour after autoclaving as a thin layer on standard cy-agar oat hulls (Fa. Roth, Karlsruhe) *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Xylan (birch) 0.5% (NH4)2SO4 0.1% Agar 1%
Preparation / Remarks: pH 7.2 Xylan (Serva pure molecular weight 25000) Pour after autoclaving as a thin layer on standard cy-agar birch (Fa. Roth, Karlsruhe) *
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