Strain identifier
BacDive ID: 21858
Type strain: 
Species: Herpetosiphon sp.
Strain Designation: Hp g225
NCBI tax ID(s): 71864 (species)
version 8.1 (current version)
General
@ref: 18281
BacDive-ID: 21858
keywords: Bacteria
description: Herpetosiphon sp. Hp g225 is a bacterium that was isolated from soil with plant residues.
NCBI tax id
- NCBI tax id: 71864
- Matching level: species
doi: 10.13145/bacdive21858.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/chloroflexota
- domain: Bacteria
- phylum: Chloroflexota
- class: Chloroflexia
- order: Herpetosiphonales
- family: Herpetosiphonaceae
- genus: Herpetosiphon
- species: Herpetosiphon sp.
- full scientific name: Herpetosiphon Holt and Lewin 1968 (Approved Lists 1980)
@ref: 18281
domain: Bacteria
phylum: Chloroflexi
class: Chloroflexi, not validated
order: Herpetosiphonales
family: Herpetosiphonaceae, not validated
genus: Herpetosiphon
species: Herpetosiphon sp.
full scientific name: Herpetosiphon sp.
strain designation: Hp g225
type strain: no
Culture and growth conditions
culture medium
| @ref | name | growth | composition |
|---|---|---|---|
| 18281 | CA13 AGAR | yes | Ingredients: Stock solution 1 (in 100ml): (NH4)2SO4 0.5g K2HPO4 7.5g Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g Basal medium: Agar 1.5% Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements |
| 18281 | CA2 AGAR | yes | Ingredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g Basal medium: Agar 1.5% Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements |
| 18281 | CHITIN 7 AGAR | yes | Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates |
| 18281 | COLE AGAR | yes | Ingredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells |
| 18281 | CY-AGAR | yes | Ingredients: Casitone(Difco) 0.3% yeast extract 0.1% CaCl2 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 (KOH) autoclaved |
| 18281 | KAN 14 AGAR | yes | Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved Combine with 1 mg/L Kanamycin-sulfate (sterilized by filtration) |
| 18281 | KAN 5 AGAR | yes | Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamine B12 0.5 µg/ml Preparation / Remarks: pH 7.2 autoclaved Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) |
| 18281 | Myx MEDIUM | yes | Ingredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416. |
| 18281 | PROBION L AGAR | yes | Ingredients: Probion L 600 (Hoechst) 0.8% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 autoclaved |
| 18281 | SKIM MILK 1 AGAR | yes | Ingredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on cy-plates. |
| 18281 | STAN 21 AGAR with Filter | yes | Ingredients: 1. Agar 1% K2HPO4 0.1% Yeast extract (Difco) 0.002% 2. (NH4)2SO4 0.1% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% MnSO4 x H2O 0.01% FeCl3 x 6H2O 0.02% MnSO4 x 7H2O 0.01% FeCl3 0.02% Traceelement-solution 1ml to 100 ml Preparation / Remarks: 1. in 2/3 of the volume after autoclaving 1 + 2 together and pour plates. Hang up a sterile-filter paper |
| 18281 | STAN 46 AGAR with Filter | yes | Ingredients: 1. Agar 1% K2HPO4 0.1% Yeast extract (Difco) 0.002% HEPES 25 mM 2. (NH4)2SO4 0.1% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% MnSO4 x H2O 0.01% FeCl3 x 6H2O 0.02% MnSO4 x 7H2O 0.01% FeCl3 0.02% Traceelement-solution 1ml to 100 ml Preparation / Remarks: 1. in 2/3 of the volume after autoclaving 1 + 2 together and pour plates. Hang up a sterile-filter paper |
| 18281 | TOLI AGAR | yes | Ingredients: CaCl2 x 2 H2O1.3g Agar (Difco)15.0g Distilled water1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak autoclaved E.coli cells |
| 18281 | VY/2 AGAR | yes | Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved |
| 18281 | XYLAN 4 AGAR | yes | Ingredients: Xylan (oat hulls 95%) 0.5% (NH4)2SO4 0.1% Agar 1% Preparation / Remarks: pH 7.2 Xylan (Serva pure molecular weight 25000) Pour after autoclaving as a thin layer on standard cy-agar oat hulls (Fa. Roth, Karlsruhe) |
| 18281 | XYLAN 5 AGAR | yes | Ingredients: Xylan (birch) 0.5% (NH4)2SO4 0.1% Agar 1% Preparation / Remarks: pH 7.2 Xylan (Serva pure molecular weight 25000) Pour after autoclaving as a thin layer on standard cy-agar birch (Fa. Roth, Karlsruhe) |
Physiology and metabolism
observation
| @ref | observation |
|---|---|
| 18281 | Ability for casein degradation |
| 18281 | Ability for chitin degradation |
| 18281 | Ability for protein degradation |
| 18281 | Ability for Yeast lysis |
| 18281 | Ability for E. coli lysis |
antibiotic resistance
| @ref | ChEBI | metabolite | is antibiotic | is resistant | resistance conc. |
|---|---|---|---|---|---|
| 18281 | 6104 | kanamycin | yes | yes | 250 µg/mL |
| 18281 | 6104 | kanamycin | yes | yes | 1 µg/mL |
Isolation, sampling and environmental information
isolation
- @ref: 18281
- sample type: soil with plant residues
- sampling date: 1991-03-01
- geographic location: near Santo Domingo
- country: Cuba
- origin.country: CUB
- continent: Middle and South America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Soil |
| #Host | #Plants |
External links
@ref: 18281
Reference
| @id | authors | title | doi/url |
|---|---|---|---|
| 18281 | Reichenbach, H. | Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 |