Cite this strain

Herpetosiphon sp. (Hp g212)

Name: Herpetosiphon sp. (Hp g212)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 21852

Type strain

Strain Designation Hp g212

Culture col. no. Hp g212

NCBI tax ID(s) 71864

Special Collections Reichenbach collection

Hp g212 is a prokaryote that was isolated from soil with plant residues.

Name and taxonomic classification

Culture Collection Numbers

Hp g212

@ref 20215

Last LPSN update 2025-12-16 09:52:15

Domain Bacillati

Phylum Chloroflexota

Class Chloroflexia

Order Herpetosiphonales

Family Herpetosiphonaceae

Genus Herpetosiphon

Full scientific name Herpetosiphon Holt and Lewin 1968 (Approved Lists 1980)

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Composition
18281	CHITIN 7 AGAR	Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates
18281	COLE AGAR	Ingredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells
18281	CY-AGAR	Ingredients: Casitone(Difco) 0.3% yeast extract 0.1% CaCl2 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 (KOH) autoclaved
18281	KAN 5 AGAR	Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamine B12 0.5 µg/ml Preparation / Remarks: pH 7.2 autoclaved Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration)
18281	Myx MEDIUM	Ingredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416.
18281	PROBION L AGAR	Ingredients: Probion L 600 (Hoechst) 0.8% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 autoclaved
18281	TOLI AGAR Kan4	Ingredients: Agar (Difco) 1.5% CaCl2 0.1% Cycloheximide 2.5 mg per 100 ml Preparation / Remarks: pH 7.2 (KOH) autoclaved w-agar with 2.5 mg of cycloheximide per 100 ml, the antibiotic is filter sterilized and added to the agar just before pouring Cycloheximide does not seem to affect growth of myxobakteria, whereas fungi and amoebae are often inhibited. Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) Pour the plates and streak autoclaved E.coli cells
18281	VY/2 AGAR	Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved

Physiology and metabolism

Observation

@ref	Observation
18281	No ability for chitin degradation
18281	No ability for protein degradation
18281	No ability for Yeast lysis
18281	Ability for E. coli lysis

Antibiotic resistance

@ref	ChEBI	Group ID	Metabolite	Resistance conc.
18281	27641	3	cycloheximide	25 µg/mL
18281	6104	3	kanamycin	250 µg/mL
18281	6104		kanamycin	250 µg/mL

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Host	#Plants	-

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent
18281	soil with plant residues	1990-03-01	southern Ceylon	Ceylon	LKA	Asia

Interaction and safety

Sequence information

Genome-based predictions

Literature

References

#18281	Reichenbach, H.: Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ . Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH:
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

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