[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g
Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g
Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g
Basal medium: Agar 1.5%
Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glycerine c) trace elements *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g
Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g
Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g
Basal medium: Agar 1.5%
Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% arabinose c) trace elements *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g
Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g
Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g
Basal medium: Agar 1.5%
Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% xylose c) trace elements *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g
Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g
Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g
Basal medium: Agar 1.5%
Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements *
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[Ref.: #18281] |
Culture medium |
|
[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g
Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g
Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g
Basal medium: Agar 1.5%
Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% galactose c) trace elements *
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[Ref.: #18281] |
Culture medium |
|
[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g
Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g
Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g
Basal medium: Agar 1.5%
Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% lactose c) trace elements *
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[Ref.: #18281] |
Culture medium |
|
[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g
Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g
Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g
Basal medium: Agar 1.5%
Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% saccharose c) trace elements *
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[Ref.: #18281] |
Culture medium |
|
[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g
Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g
Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g
Basal medium: Agar 1.5%
Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% fructose c) trace elements *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5%
Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml
Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Casitone(Difco) 0.3% yeast extract 0.1% CaCl2 0.1% Agar 1.5%
Preparation / Remarks: pH 7.2 (KOH) autoclaved *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Casitone (Difco) 0.1% Yeast extract (Difco) 0.05% CaCl2 x 2H2O 0.02% MgSO4 x 7H2O 0.05% Agar 1.5% KNO3 0.1% Preparation / Remarks: pH 7.2 after autoclaving add: glucose 0.2% (autoclaved separately) 50 mg/L Kanamycin-sulfate (sterilized by filtration) *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Casitone (Difco) 0.1% Yeast extract (Difco) 0.05% CaCl2 x 2H2O 0.02% MgSO4 x 7H2O 0.05% Agar 1.5% KNO3 0.1% Kanamycin-sulfate 100 mg/L
Preparation / Remarks: pH 7.2 after autoclaving add: Glucose 0.2% (autoclaved separately) 100 mg/L Kanamycin-sulfate (sterilized by filtration) *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Casitone (Difco) 0.1% Yeast extract (Difco) 0.05% CaCl2 x 2H2O 0.02% MgSO4 x 7H2O 0.05% Agar 1.5% KNO3 0.1%
Preparation / Remarks: pH 7.2 after autoclaving add: Glucose 0.2% (autoclaved separately) 250 mg/L Kanamycin-sulfate (sterilized by filtration) *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamine B12 0.5 µg/ml
Preparation / Remarks: pH 7.2 autoclaved Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416. *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Probion L 600 (Hoechst) 0.8% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% Agar 1.5%
Preparation / Remarks: pH 7.2 autoclaved *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml
Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on CYG2-plates. *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 %
Preparation / Remarks: pH 7.2 autoclaved *
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[Ref.: #18281] |
Culture medium |
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[Ref.: #18281] |
Culture medium growth |
* |
[Ref.: #18281] |
Culture medium composition |
expand / minimize * |
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Ingredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml
Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on CA2-plates. *
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