Covering agar (thin layer):
MgSO4 x 7H2O 0.1%
K2HPO4 0.02%
Agar 1.5%
Preparation / Remarks:
pH 7.2
After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates
Ingredients:
CaCl2 x 2 H2O1.3g
Agar (Difco)15.0g
Distilled water1000.0ml
Preparation / Remarks:
pH 7.2 (KOH)
autoclaved
Pour the plates and streak autoclaved E.coli cells
Reichenbach, H.: Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ .
Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH:
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When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
Isabel Schober, Julia Koblitz, Joaquim Sardà Carbasse, Christian Ebeling, Marvin Leon Schmidt, Adam Podstawka, Rohit Gupta, Vinodh Ilangovan, Javad Chamanara, Jörg Overmann, Lorenz Christian Reimer
Nucleic Acids Research; database issue 2025.