Strain identifier
BacDive ID: 21852
Type strain: 
Species: Herpetosiphon sp.
Strain Designation: Hp g212
NCBI tax ID(s): 71864 (species)
version 8.1 (current version)
General
@ref: 18281
BacDive-ID: 21852
keywords: Bacteria
description: Herpetosiphon sp. Hp g212 is a bacterium that was isolated from soil with plant residues.
NCBI tax id
- NCBI tax id: 71864
- Matching level: species
doi: 10.13145/bacdive21852.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/chloroflexota
- domain: Bacteria
- phylum: Chloroflexota
- class: Chloroflexia
- order: Herpetosiphonales
- family: Herpetosiphonaceae
- genus: Herpetosiphon
- species: Herpetosiphon sp.
- full scientific name: Herpetosiphon Holt and Lewin 1968 (Approved Lists 1980)
@ref: 18281
domain: Bacteria
phylum: Chloroflexi
class: Chloroflexi, not validated
order: Herpetosiphonales
family: Herpetosiphonaceae, not validated
genus: Herpetosiphon
species: Herpetosiphon sp.
full scientific name: Herpetosiphon sp.
strain designation: Hp g212
type strain: no
Culture and growth conditions
culture medium
| @ref | name | growth | composition |
|---|---|---|---|
| 18281 | CHITIN 7 AGAR | yes | Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates |
| 18281 | COLE AGAR | yes | Ingredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells |
| 18281 | CY-AGAR | yes | Ingredients: Casitone(Difco) 0.3% yeast extract 0.1% CaCl2 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 (KOH) autoclaved |
| 18281 | KAN 5 AGAR | yes | Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamine B12 0.5 µg/ml Preparation / Remarks: pH 7.2 autoclaved Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) |
| 18281 | Myx MEDIUM | yes | Ingredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416. |
| 18281 | PROBION L AGAR | yes | Ingredients: Probion L 600 (Hoechst) 0.8% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 autoclaved |
| 18281 | TOLI AGAR Kan4 | yes | Ingredients: Agar (Difco) 1.5% CaCl2 0.1% Cycloheximide 2.5 mg per 100 ml Preparation / Remarks: pH 7.2 (KOH) autoclaved w-agar with 2.5 mg of cycloheximide per 100 ml, the antibiotic is filter sterilized and added to the agar just before pouring Cycloheximide does not seem to affect growth of myxobakteria, whereas fungi and amoebae are often inhibited. Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) Pour the plates and streak autoclaved E.coli cells |
| 18281 | VY/2 AGAR | yes | Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved |
Physiology and metabolism
observation
| @ref | observation |
|---|---|
| 18281 | No ability for chitin degradation |
| 18281 | No ability for protein degradation |
| 18281 | No ability for Yeast lysis |
| 18281 | Ability for E. coli lysis |
antibiotic resistance
| @ref | ChEBI | group ID | metabolite | is antibiotic | is resistant | resistance conc. |
|---|---|---|---|---|---|---|
| 18281 | 6104 | 3 | kanamycin | yes | yes | 250 µg/mL |
| 18281 | 27641 | 3 | cycloheximide | yes | yes | 25 µg/mL |
| 18281 | 6104 | kanamycin | yes | yes | 250 µg/mL |
Isolation, sampling and environmental information
isolation
- @ref: 18281
- sample type: soil with plant residues
- sampling date: 1990-03-01
- geographic location: southern Ceylon
- country: Ceylon
- origin.country: LKA
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Soil |
| #Host | #Plants |
External links
@ref: 18281
Reference
| @id | authors | title | doi/url |
|---|---|---|---|
| 18281 | Reichenbach, H. | Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 |