Name: Vibrio cholerae VL 3029
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 131086

Type strain:

Species: Vibrio cholerae

Strain Designation: VL 3029

Culture col. no.: DSM 101014, NCTC 11348, WDCM 00136, CCUG 67718, CIP 111241

Strain history: CIP <- 2016, CCUG <- NCTC <- A.L. Furniss, PHL Maidstone, Kent, England

NCBI tax ID(s): 666 (species)

Special Collections

Other strains from Vibrio cholerae

Section

Vibrio cholerae VL 3029 is an aerobe, Gram-negative, motile bacterium that was isolated from human faeces.

Gram-negative
motile
rod-shaped
aerobe
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	"Vibrionales"
	Family	*Vibrionaceae*
	Genus	*Vibrio*
	Species	**Vibrio cholerae**
	Full Scientific Name (LPSN)	Vibrio cholerae Pacini 1854 (Approved Lists 1980)

Synonym

Vibrio albensis

Information on morphological and physiological properties Morphology

[Ref.: #118252]	Gram stain	negative

[Ref.: #118252]	Cell shape	rod-shaped

[Ref.: #118252]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #22378]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #22378]

Culture medium growth

[Ref.: #22378]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22378]

Culture medium composition

expand / minimize

[Ref.: #22378]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #22378]

Culture medium growth

[Ref.: #22378]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22378]

Culture medium composition

expand / minimize

[Ref.: #118252]

Culture medium

CIP Medium 72

[Ref.: #118252]

Culture medium growth

[Ref.: #118252]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #22378]			growth	28 °C
[Ref.: #63431]			growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #63431]

Oxygen tolerance

aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE, API 20E, API ID32E
[Ref.: #68374]	cellobiose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68374]	D-arabitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	D-galacturonic acid ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	fermentation	* from API 20NE
[Ref.: #68374]	D-glucose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68374]	D-mannitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE, API 20E
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68374]	L-arabinose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68374]	L-arabitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	L-rhamnose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #68374]	lysine ChEBI	-	degradation	* from API ID32E
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68374]	malonate ChEBI	-	assimilation	* from API ID32E
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68374]	maltose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68374]	myo-inositol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E, API ID32E
[Ref.: #68374]	palatinose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	ribitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	sorbitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #68374]	sucrose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	trehalose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68369]	tryptophan ChEBI	+	energy source	* from API 20NE, API 20E, API ID32E
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE, API 20E, API ID32E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68368]		hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68374]		indole ChEBI	yes	* from API ID32E, API 20NE, API 20E

	Physiological tests	Metabolite	Indole test
[Ref.: #68374]		indole ChEBI	+	* from API ID32E, API 20NE, API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68374]	alpha-galactosidase	-	3.2.1.22	* from API ID32E
[Ref.: #68374]	alpha-glucosidase	-	3.2.1.20	* from API ID32E
[Ref.: #68374]	alpha-maltosidase	+		* from API ID32E
[Ref.: #68374]	arginine dihydrolase	-	3.5.3.6	* from API ID32E, API 20NE, API 20E
[Ref.: #68374]	beta-galactosidase	+	3.2.1.23	* from API ID32E, API 20E
[Ref.: #68374]	beta-glucosidase	-	3.2.1.21	* from API ID32E, API 20NE
[Ref.: #68374]	beta-glucuronidase	-	3.2.1.31	* from API ID32E
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE, API 20E
[Ref.: #68369]	gelatinase	+		* from API 20NE, API 20E
[Ref.: #68374]	L-aspartate arylamidase	+	3.4.11.21	* from API ID32E
[Ref.: #68374]	lipase	+		* from API ID32E
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68374]	lysine decarboxylase	-	4.1.1.18	* from API ID32E
[Ref.: #68374]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API ID32E
[Ref.: #68374]	ornithine decarboxylase	+	4.1.1.17	* from API ID32E, API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68374]	urease	-	3.5.1.5	* from API ID32E, API 20NE, API 20E
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #63431]

Fatty acid	Percentage	ECL
C16:1 ω7c	35.9	15.819
C16:0	18	16
C18:1 ω7c /12t/9t	14.5	17.824
C14:0	6.5	14
C12:0 3OH	4.6	13.455
C14:0 3OH/C16:1 ISO I	3.3	15.485
C16:1 ω7c/C15:0 ISO 2OH	3	15.85
C15:0 ISO 3OH	2.7	16.135
Unidentified	2.6	16.303
Unidentified	2.2	18.15
C18:2 ω6,9c/C18:0 ANTE	1.5	17.724
C16:1 ω9c	0.9	15.774
C17:1 ω8c	0.8	16.792
C18:1 ω9c	0.7	17.769
C18:0	0.6	18
unknown 12.486	0.5	12.486
11 methyl 18:1 ω7c	0.5	18.081
C15:0	0.5	15
C13:0 ISO 2OH	0.4	13.814
unknown 15.273	0.4	15.273

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #22378]	17957	+	-	+	+	+	-	-	-	+	+	+	+/-	+/-	-	-	-	+	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #63431]	6300	+	-	+	+	+	-	-	-	+	-	+	+	+	-	-	-	+	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #22378]	24556	+	+	+	-	-	-	+	+	+	-	+	+	+	+	+	-	-	+	+	-	+
[Ref.: #22378]	23535	+	+	+	-	-	-	+	+	+	-	+	+	+	+	+	-	-	+	+	-	+

API® ID32E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Ornithine decarboxylaseODC	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Urease/urea hydrolysisURE	Acid from L-arabitolLARL	Acid from D-galacturonic acidGAT	Acid from 5-Ketogluconate5KG	LipaseLIP	Phenol red (Acidification)RP	beta-Glucosidasebeta GLU	Acid from D-mannitolMAN	Acid from maltoseMAL	Acid from D-adonitolADO	Acid from palatinosePLE	beta-Glucuronidasebeta GUR	MalonateMNT	Indole productionIND	N-Acetyl-beta-Glucosaminidasebeta NAG	beta-Galactosidasebeta GAL	Acid from D-glucoseGLU	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	alpha-Glucosidasealpha GLU	alpha-Galactosidasealpha GAL	Acid from trehaloseTRE	Acid from L-rhamnoseRHA	Acid from inositolINO	Acid from cellobioseCEL	Acid from sorbitolSOR	alpha-MaltosidasealphaMAL	L-aspartic acid arylamidaseAspA
[Ref.: #63431]	1986	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22378]	Sample type/isolated from	human faeces
[Ref.: #22378]	Country	United Kingdom
[Ref.: #22378]	Country ISO 3 Code	GBR
[Ref.: #22378]	Continent	Europe

[Ref.: #63431]	Sample type/isolated from	Human feces
[Ref.: #63431]	Sampling date	1980-01-01
[Ref.: #63431]	Geographic location (country and/or sea, region)	Maidstone,Kent
[Ref.: #63431]	Country	United Kingdom
[Ref.: #63431]	Country ISO 3 Code	GBR
[Ref.: #63431]	Continent	Europe

[Ref.: #118252]	Sample type/isolated from	Human, Faeces
[Ref.: #118252]	Sampling date	1980
[Ref.: #118252]	Geographic location (country and/or sea, region)	England
[Ref.: #118252]	Country	United Kingdom
[Ref.: #118252]	Country ISO 3 Code	GBR
[Ref.: #118252]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #22378]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #118252]	Biosafety level	2	Risk group (French classification)

Availability in culture collections External links

[Ref.: #22378]

Culture collection no.

DSM 101014, NCTC 11348, WDCM 00136, CCUG 67718, CIP 111241

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22378

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101014

#63431 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 67718

#68368

Automatically annotated from API 20E .

#68369

Automatically annotated from API 20NE .

#68374

Automatically annotated from API ID32E .

#118252 Collection of Institut Pasteur ; Curators of the CIP; CIP 111241
* These data were automatically processed and therefore are not curated

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