Name: Vibrio cholerae strain 3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130763

Type strain:

Species: Vibrio cholerae

Strain Designation: strain 3, J4308

Culture col. no.: CCUG 9118, DSM 100200, ATCC 14035, CCUG 9118, CIP 62.13, NCTC 8021, CECT 514

Strain history: <- CIP; CIP 62.13 <- NCTC; NCTC 8021 <- H.J. Bensted, PHLS - Colindale; strain 3

NCBI tax ID(s): 1408478 (strain), 666 (species)

Other strains from Vibrio cholerae

Section

Vibrio cholerae strain 3 is a mesophilic bacterium that has multiple antibiotic resistances.

antibiotic resistance
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	"Vibrionales"
	Family	*Vibrionaceae*
	Genus	*Vibrio*
	Species	**Vibrio cholerae**
	Full Scientific Name (PNU)	Vibrio cholerae Pacini 1854 (Approved Lists 1980)

Synonym

Vibrio albensis

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21977]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #21977]

Culture medium growth

[Ref.: #21977]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #21977]

Culture medium composition

expand / minimize

[Ref.: #21977]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #21977]

Culture medium growth

[Ref.: #21977]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #39747]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #39747]

Culture medium growth

[Ref.: #39747]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #21977]			growth	30 °C
[Ref.: #39747]			growth	30 °C

[Ref.: #21977]	Temperature range	mesophilic
[Ref.: #39747]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Reference

[Ref.: #21977]
Mueller-Hinton Agar
30

aerob
yes
Inhibition zone diameter in mm
16
resistant0
20
32
34-36
30
26
44
34
28
34-36
38
24
24
resistant0
22
8
42
46
12-14
36
28
resistant0
14-16
24-26
20-22
32
42
10
10
40
28
resistant0
14
resistant0
32

[Ref.: #21977]
Mueller-Hinton Agar
30

aerob
yes
Inhibition zone diameter in mm
16
resistant0
20
30
32-34
30
24
42
34
28
36
36
22
22
resistant0
20
resistant0
40
40
14
34
30
resistant0
16
24
20
34
38-40
10
12-14
36-38
30
12-14
16
10
34

[Ref.: #21977]
Mueller-Hinton Agar
30

aerob
yes
Inhibition zone diameter in mm
16
resistant0
20
30
32-34
30
24
42
34
28
36
36
22
22
resistant0
20
resistant0
40
40
14
34
30
resistant0
16
24
20
34
38-40
10
12-14
36-38
30
12-14
16
10
34

Only first 15 entries are displayed. Click here to see all.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE, API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	fermentation	* from API 20NE, API 20E
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE, API 20E
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #68369]	tryptophan ChEBI	+	energy source	* from API 20NE, API 20E
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE, API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite
[Ref.: #68368]	acetoin ChEBI	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	* from API 20E
[Ref.: #68369]	indole ChEBI	* from API 20NE, API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68369]		indole ChEBI		+	* from API 20NE, API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE, API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE, API 20E
[Ref.: #68369]	gelatinase	+		* from API 20NE, API 20E
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE, API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #21977]	15309	+	-	+	+	+	-	-	-	+	+	+	+	+	-	-	-	+	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #21977]	14143	+	-	+	+	+	-	-	-	+	+	+	+	+	-	-	-	+	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #21977]	14109	+	-	+	+	+	-	-	-	+	+	+	+	+	-	-	-	+	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #21977]	20904	+	+	+	-	-	-	+	+	+	-	+	+	+	+	+	-	-	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21977]	Geographic location (country and/or sea, region)	unknown?
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21977]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21977]	V.cholerae (CECT 514 T) 16S rRNA gene	X76337	1538	ENA	666 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Vibrio cholerae ATCC 14035 ATCC 14035	GCA_000621645	scaffold	GenBank	1408478 tax ID	*
[Ref.: #66792]	Vibrio cholerae CECT 514	GCA_013155105	complete	GenBank	666 tax ID	*
[Ref.: #66792]	Vibrio cholerae ATCC 14035	1408478.3	wgs	PATRIC	1408478 tax ID	*
[Ref.: #66792]	Vibrio cholerae strain CECT 514	666.5088	complete	PATRIC	666 tax ID	*
[Ref.: #66792]	Vibrio cholerae ATCC 14035	2556921658	draft	JGI IMG/M	1408478 tax ID	*

Availability in culture collections External links

[Ref.: #21977]

Culture collection no.

CCUG 9118, DSM 100200, ATCC 14035, CCUG 9118, CIP 62.13, NCTC 8021, CECT 514

[Ref.: #20218]

Associated Passport(s) in StrainInfo

97285, 156129, 234997, 97286

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	[Causative agent of the so-called "light disease of shrimps" is luminescent Vibrio cholerae non-O1].	Shimada T, Arakawa E, Itoh K, Kosako Y, Okitsu T, Yamai S, Nishino M, Nakajima T	Nihon Saikingaku Zasshi	10.3412/jsb.50.863	1995	*
Metabolism	Characterization of the melanogenic system in Vibrio cholerae, ATCC 14035.	Ruzafa C, Sanchez-Amat A, Solano F	Pigment Cell Res	10.1111/j.1600-0749.1995.tb00656.x	1995	*
Metabolism	Comparative tyrosine degradation in Vibrio cholerae strains. The strain ATCC 14035 as a prokaryotic melanogenic model of homogentisate-releasing cell.	Sanchez-Amat A, Ruzafa C, Solano F	Comp Biochem Physiol B Biochem Mol Biol	10.1016/s0305-0491(98)00028-5	1998	*
Enzymology	Role of surface proteins in Vibrio cholerae attachment to chitin.	Tarsi R, Pruzzo C	Appl Environ Microbiol	10.1128/AEM.65.3.1348-1351.1999	1999	*
Pathogenicity	Response of pathogenic Vibrio species to high hydrostatic pressure.	Berlin DL, Herson DS, Hicks DT, Hoover DG	Appl Environ Microbiol	10.1128/AEM.65.6.2776-2780.1999	1999	*
Enzymology	Comparison of two recombinant systems for expression of cholera toxin B subunit from Vibrio cholerae.	Boustanshenas M, Bakhshi B, Ghorbani M, Norouzian D	Indian J Med Microbiol	10.4103/0255-0857.108705	2013	*
Enzymology	A single point mutation within the coding sequence of cholera toxin B subunit will increase its expression yield.	Bakhshi B, Boustanshenas M, Ghorbani M	Iran Biomed J	10.6091/ibj.1165.2014	2014	*
Phylogeny	Streptomyces thermoviolaceus SRC3 strain as a novel source of the antibiotic adjuvant streptazolin: A statistical approach toward the optimized production.	Djinni I, Djoudi W, Souagui S, Rabia F, Rahmouni S, Mancini I, Kecha M	J Microbiol Methods	10.1016/j.mimet.2018.04.008	2018	*
Pathogenicity	Vibrio cholerae toxin coregulated pilus provokes inflammatory responses in Coculture model of Caco-2 and peripheral blood mononuclear cells (PBMC) leading to increased colonization.	Ghasemi M, Bakhshi B, Khashei R, Soudi S, Boustanshenas M	Microbiol Immunol	10.1111/1348-0421.12889	2021	*
Metabolism	Pufferfish (Tetraodon cutcutia) Sampled from a Freshwater River Serves as an Intermediate Reservoir of a Sucrose Nonfermenting Variant of Vibrio cholerae PS-4.	Das L, Deb S, Arakawa E, Yamasaki S, Das SK	Microbiol Spectr	10.1128/spectrum.01221-21	2022	*
	The hows and whys of constructing a native recombinant cholera vaccine.	Boustanshenas M, Bakhshi B	Bioengineered	10.4161/bioe.26420	2013	*
	Growth Inhibitory Effect of Lactocare on Vibrio cholerae.	Asadi M, Fazeli MR, Sabokbar A	Iran J Pathol		2018	*
	Capsaicin inhibitory effects on Vibrio cholerae toxin genes expression.	Erfanimanesh S, Eslami G, Taherpour A, Hashemi A	Avicenna J Phytomed		2019	*
	Genetic Diversity of ctxB Gene Among Classical O1 and El Tor Strains of Vibrio cholerae using High-Resolution Melting Curve Analysis.	Mahboobi M, Mirnejad R, Sedighian H, Piranfar V, Imani Fooladi AA	Iran J Pathol	10.30699/ijp.2020.127793.2393	2020	*
	Investigation of the Antibacterial and Antibiofilm Activity of Selenium Nanoparticles against Vibrio cholerae as a Potent Therapeutics.	Bagheri-Josheghani S, Bakhshi B	Can J Infect Dis Med Microbiol	10.1155/2022/3432235	2022	*
	Bacterial community structure and bacterial isolates having antimicrobial potential in shrimp pond aquaculture.	Ali S, Xie J, Zada S, Hu Z, Zhang Y, Cai R, Wang H	AMB Express	10.1186/s13568-022-01423-9	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#21977

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100200

#39747 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#68369

Automatically annotated from API 20NE .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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