Strain identifier

BacDive ID: 131086

Type strain: No

Species: Vibrio cholerae

Strain Designation: VL 3029

Strain history: CIP <- 2016, CCUG <- NCTC <- A.L. Furniss, PHL Maidstone, Kent, England

NCBI tax ID(s): 666 (species)

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General

@ref: 22378

BacDive-ID: 131086

DSM-Number: 101014

keywords: Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Vibrio cholerae VL 3029 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from human faeces.

NCBI tax id

  • NCBI tax id: 666
  • Matching level: species

strain history

@refhistory
22378<- NCTC <- A. L. Furniss, PHL Maidstone, Kent, England
118252CIP <- 2016, CCUG <- NCTC <- A.L. Furniss, PHL Maidstone, Kent, England

doi: 10.13145/bacdive131086.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Vibrionales
  • family: Vibrionaceae
  • genus: Vibrio
  • species: Vibrio cholerae
  • full scientific name: Vibrio cholerae Pacini 1854 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Vibrio albensis

@ref: 22378

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Vibrionales, not validated

family: Vibrionaceae

genus: Vibrio

species: Vibrio cholerae

full scientific name: Vibrio cholerae Pacini 1854

strain designation: VL 3029

type strain: no

Morphology

cell morphology

  • @ref: 118252
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22378TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
22378COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
118252CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
22378positivegrowth28mesophilic
63431positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 63431
  • oxygen tolerance: aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose+fermentation
6836927897tryptophan+energy source
6836917632nitrate+reduction
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
683685291gelatin+hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis
6837418257ornithine+degradation
6837429016arginine-hydrolysis
6837425094lysine-degradation
6837416199urea-hydrolysis
6837418403L-arabitol-builds acid from
6837418024D-galacturonic acid-builds acid from
68374Potassium 5-ketogluconate-builds acid from
6837416899D-mannitol-builds acid from
6837417306maltose-builds acid from
6837415963ribitol-builds acid from
6837418394palatinose-builds acid from
6837415792malonate-assimilation
6837427897tryptophan+energy source
6837417634D-glucose-builds acid from
6837417992sucrose-builds acid from
6837430849L-arabinose-builds acid from
6837418333D-arabitol-builds acid from
6837427082trehalose-builds acid from
6837462345L-rhamnose-builds acid from
6837417268myo-inositol-builds acid from
6837417057cellobiose-builds acid from
6837430911sorbitol-builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
6837435581indoleyes
6836935581indoleyes
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetaboliteindole test
6837435581indole+
6836935581indole+
6836835581indole+

enzymes

@refvalueactivityec
68374L-aspartate arylamidase+3.4.11.21
68374alpha-maltosidase+
68374alpha-galactosidase-3.2.1.22
68374alpha-glucosidase-3.2.1.20
68374beta-galactosidase+3.2.1.23
68374N-acetyl-beta-glucosaminidase-3.2.1.52
68374beta-glucuronidase-3.2.1.31
68374beta-glucosidase-3.2.1.21
68374lipase+
68374urease-3.5.1.5
68374lysine decarboxylase-4.1.1.18
68374arginine dihydrolase-3.5.3.6
68374ornithine decarboxylase+4.1.1.17
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68368cytochrome oxidase+1.9.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    63431C14:06.514
    63431C15:00.515
    63431C16:01816
    63431C18:00.618
    6343111 methyl 18:1 ω7c0.518.081
    63431C12:0 3OH4.613.455
    63431C13:0 ISO 2OH0.413.814
    63431C14:0 3OH/C16:1 ISO I3.315.485
    63431C15:0 ISO 3OH2.716.135
    63431C16:1 ω7c35.915.819
    63431C16:1 ω7c/C15:0 ISO 2OH315.85
    63431C16:1 ω9c0.915.774
    63431C17:1 ω8c0.816.792
    63431C18:1 ω7c /12t/9t14.517.824
    63431C18:1 ω9c0.717.769
    63431C18:2 ω6,9c/C18:0 ANTE1.517.724
    63431Unidentified2.616.303
    63431Unidentified2.218.15
    63431unknown 12.4860.512.486
    63431unknown 15.2730.415.273
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
22378+-+++---++++/-+/----+---+
63431+-+++---+-+++---+---+

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
22378+++---+++-+++++--++-+
22378+++---+++-+++++--++-+

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
63431+------+--------+-+-----------++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic location
22378human faecesUnited KingdomGBREurope
63431Human fecesUnited KingdomGBREurope1980-01-01Maidstone,Kent
118252Human, FaecesUnited KingdomGBREurope1980England

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
223782Risk group (German classification)
1182522Risk group (French classification)

External links

@ref: 22378

culture collection no.: DSM 101014, NCTC 11348, WDCM 00136, CCUG 67718, CIP 111241

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22378Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-101014Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101014)
63431Curators of the CCUGhttps://www.ccug.se/strain?id=67718Culture Collection University of Gothenburg (CCUG) (CCUG 67718)
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE
68374Automatically annotated from API ID32E
118252Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20111241Collection of Institut Pasteur (CIP 111241)