Clostridium sporogenes (DSM 29422, 2PG-426-CC-2)

Name: Clostridium sporogenes (DSM 29422, 2PG-426-CC-2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 130932

Type strain

Strain Designation 2PG-426-CC-2

Culture col. no. DSM 29422 2PG-426-CC-2

NCBI tax ID(s) 1509

Special Collections DSMZ Mouse DZIF

History <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; 2PG-426-CC-2

Links

Clostridium sporogenes 2PG-426-CC-2 is an anaerobe bacterium that was isolated from caecal content; TNF^deltaARE/+ C57BL/6 mouse.

anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 397852

DSM 29422

@ref 20215

Last LPSN update 2026-02-09 11:31:20

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Clostridiaceae

Genus Clostridium

Species Clostridium sporogenes

Full scientific name Clostridium sporogenes (Metchnikoff 1908) Bergey et al. 1923 (Approved Lists 1980)

Synonyms (1)

"Bacillus sporogenes"

BacDive ID	Other strains from **Clostridium sporogenes** (26)	Type strain
2710	C. sporogenes McClung 2004, 388, DSM 795, ATCC 3584, IFO ... (type strain)
2707	C. sporogenes W28delta, DSM 633
2708	C. sporogenes H6j, DSM 634
2709	C. sporogenes McClung 2003, 3679, Cameron's putrefactive ...
2711	C. sporogenes McClung 2006, 2006, DSM 1446, ATCC 11437, ...
2712	C. sporogenes SR 5, SR5, DSM 1664, ATCC 19404, CIP 79.3, ...
2714	C. sporogenes DSM 46278, IMET 10860, SG 272
2715	C. sporogenes 15Rga, DSM 46279, IMET 10863
100269	C. sporogenes ST033277(HKI),
134401	C. sporogenes AU297, CIP 108177
136740	C. sporogenes GO 1, CIP 60.53
137136	C. sporogenes AU298, CIP 108178
138778	C. sporogenes 51, CIP 79.39
141391	C. sporogenes CCUG 1388
142431	C. sporogenes CCUG 12462
151851	C. sporogenes CCUG 45049
152722	C. sporogenes CCUG 47475
152930	C. sporogenes CCUG 48045
153019	C. sporogenes CCUG 48328
154094	C. sporogenes CCUG 52331
156952	C. sporogenes CCUG 67315
165625	C. sporogenes JCM 7831, ATCC 6015, IAM 19003, NCTC 6084
165628	C. sporogenes JCM 7835, IAM 19004
165629	C. sporogenes JCM 7836, IAM 19016
165634	C. sporogenes JCM 7841, IAM 19005
165635	C. sporogenes JCM 7849, IAM 19234

Morphology

Colony morphology

22224

Incubation period2-3 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
22224	FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203)		Medium recipe at MediaDive	Name: FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203) Composition: Horse blood 100.0 g/l Fastidious Anaerobe Agar 45.7 g/l Distilled water
22224	PYG MEDIUM (MODIFIED) (DSMZ Medium 104)		Medium recipe at MediaDive	Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
22224	positive	growth	37

Physiology and metabolism

Oxygen tolerance

22224

Oxygen toleranceanaerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68380	29016 ChEBI	arginine	+	hydrolysis	from API rID32A
68380	16024 ChEBI	D-mannose	-	fermentation	from API rID32A
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
68380	16634 ChEBI	raffinose	-	fermentation	from API rID32A
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A

Enzymes

@ref	Value	Activity	Ec
68380	alanine arylamidase	-	3.4.11.2	from API rID32A
68380	alkaline phosphatase	-	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68380	alpha-galactosidase	-	3.2.1.22	from API rID32A
68380	alpha-glucosidase	+	3.2.1.20	from API rID32A
68380	arginine dihydrolase	+	3.5.3.6	from API rID32A
68380	beta-galactosidase	-	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68380	beta-glucosidase	-	3.2.1.21	from API rID32A
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	-		from API rID32A
68380	histidine arylamidase	-		from API rID32A
68380	L-arginine arylamidase	-		from API rID32A
68380	leucine arylamidase	-	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
68380	phenylalanine arylamidase	-		from API rID32A
68380	proline-arylamidase	+	3.4.11.5	from API rID32A
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
68380	tyrosine arylamidase	-		from API rID32A
68380	urease	-	3.5.1.5	from API rID32A

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
22224	-	+	-	-	-	+	-	-	-	-	-	-	-	-	-	+/-	-	-	+	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Muridae (Mouse/Rat)
#Host Body-Site	#Gastrointestinal tract	#Large intestine
#Host Body Product	#Gastrointestinal tract	#Caecal content

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
22224	caecal content; TNF^deltaARE/+ C57BL/6 mouse	Freising	Germany	DEU	Europe

Taxonmaps

69479

Global distribution of 16S sequence KU196083 (>99% sequence identity) for Clostridium from Microbeatlas

Aquatic Samples	3962
Soil Samples	4923
Animal Samples	31978
Plant Samples	1109
Total Samples	41972

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
22224	2	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2462288v1 assembly for Clostridium sporogenes DSM 29422	contig	GCA_024622885	1509.260	8071637400	1509	50.43

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
22224	Clostridium sporogenes strain DSM 29422 16S ribosomal RNA gene, partial sequence	KU196083	1441		1509

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Clostridium sporogenes DSM 29422
NCBI: GCA_024622885.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	62.51	no
125438	anaerobic	anaerobicⓘ	yes	78.77	yes
125438	aerobic	aerobicⓘ	no	89.27	no
125438	spore-forming	spore-formingⓘ	yes	72.17	no
125438	thermophilic	thermophileⓘ	no	94.71	yes
125438	flagellated	motile2+ⓘ	yes	77.64	no

Literature

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#22224	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29422
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68380	Automatically annotated from API rID32A .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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