Name: Clostridium sporogenes SR 5
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2712

Type strain:

Species: Clostridium sporogenes

Strain Designation: SR 5, SR5

Culture col. no.: DSM 1664, ATCC 19404, CIP 79.3, NCIB 532, NCTC 532, WDCM 00008, NCIMB 532, LMG 10143, CCM 4409, CECT 485, CMCC (B) 64941

Strain history: CIP <- 1979, ATCC <- NCTC 532 <- 1920, M. Robertson, Lister Inst., London, UK: strain SR5

NCBI tax ID(s): 1509 (species)

SI-ID 1974

LMG 10143, ATCC 19404, NCIB 532, NCTC 532, CCUG 18371, CCUG 7489, NCIMB 532, NCDO1710, CCM 4409, CIP 79.03, CIP 79.3, LMD 85.28, CECT 485, CCRC 11258, NCFB 1710, NCCB 85028, BCRC 11258, CCT 1448, NCAIM B.02002, NCAIM B.02007, NCIM 5125, ACM 5049, CNCTC 6194, CGMCC 1.2417, VTT E-96697, DSM 1664

Other strains from Clostridium sporogenes

Section

Clostridium sporogenes SR 5 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from gas gangrene.

motile
spore-forming
anaerobe
mesophilic
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Clostridium*
	Species	**Clostridium sporogenes**
	Full Scientific Name (LPSN)	Clostridium sporogenes (Metchnikoff 1908) Bergey et al. 1923 (Approved Lists 1980)

Synonym

"Bacillus sporogenes"

Information on morphological and physiological properties Morphology

[Ref.: #123273]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #123273]	Cell shape	rod-shaped

[Ref.: #123273]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %91.869

[Ref.: #123273]

Hemolysis ability

Information on culture and growth conditions Culture and growth conditions

[Ref.: #845]

Culture medium

CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)

[Ref.: #845]

Culture medium growth

[Ref.: #845]

Culture medium link

Medium recipe at MediaDive

[Ref.: #845]

Culture medium composition

expand / minimize

[Ref.: #845]

Culture medium

FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a)

[Ref.: #845]

Culture medium growth

[Ref.: #845]

Culture medium link

Medium recipe at MediaDive

[Ref.: #845]

Culture medium composition

expand / minimize

[Ref.: #36205]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #36205]

Culture medium growth

[Ref.: #36205]

Culture medium composition

expand / minimize

[Ref.: #845]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #845]

Culture medium growth

[Ref.: #845]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123273]

Culture medium

CIP Medium 20

[Ref.: #123273]

Culture medium growth

[Ref.: #123273]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #845]	growth	37 °C
[Ref.: #36205]	growth	37 °C
[Ref.: #123273]	growth	37 °C

[Ref.: #845]	Temperature range	mesophilic
[Ref.: #36205]	Temperature range	mesophilic
[Ref.: #123273]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #845]	Oxygen tolerance	anaerobe
[Ref.: #123273]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.998

[Ref.: #123273]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %95
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	+	hydrolysis	* from API rID32A
[Ref.: #68367]	cellobiose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-glucose ChEBI	+	builds acid from	* from API 20A
[Ref.: #68367]	D-mannitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-mannose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68367]	D-xylose ChEBI	-	builds acid from	* from API 20A
[Ref.: #123273]	esculin ChEBI	+	hydrolysis
[Ref.: #68367]	esculin ChEBI	+	hydrolysis	* from API 20A
[Ref.: #68367]	gelatin ChEBI	+	hydrolysis	* from API 20A
[Ref.: #68367]	glycerol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	L-arabinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68367]	L-rhamnose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	lactose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	maltose ChEBI	+	builds acid from	* from API 20A
[Ref.: #68367]	melezitose ChEBI	-	builds acid from	* from API 20A
[Ref.: #123273]	nitrate ChEBI	-	reduction
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #123273]	nitrite ChEBI	+	reduction
[Ref.: #68367]	raffinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68367]	salicin ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	sorbitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	sucrose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	trehalose ChEBI	+	builds acid from	* from API 20A
[Ref.: #68367]	tryptophan ChEBI	-	energy source	* from API 20A, API rID32A
[Ref.: #68367]	urea ChEBI	-	hydrolysis	* from API 20A, API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #123273]		indole ChEBI	no
[Ref.: #68367]		indole ChEBI	no	* from API 20A, API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A, API 20A

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #123273]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym, API rID32A
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym, API rID32A
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68380]	arginine dihydrolase	+	3.5.3.6	* from API rID32A
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68367]	beta-glucosidase	+	3.2.1.21	* from API 20A
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API rID32A
[Ref.: #123273]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68367]	gelatinase	+		* from API 20A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123273]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API rID32A
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #123273]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123273]	oxidase	-
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	+	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #123273]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A, API 20A
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Indole productionIND	Urease/urea hydrolysisURE	Acid from D-glucoseGLU	Acid from D-mannitolMAN	Acid from lactoseLAC	Acid from sucroseSAC	Acid from maltoseMAL	Acid from salicinSAL	Acid from D-xyloseXYL	Acid from L-arabinoseARA	Gelatinase/gelatin hydrolysisGEL	Esculin hydrolysis/beta-glucosidaseESC	Acid from glycerolGLY	Acid from cellobioseCEL	Acid from D-mannoseMNE	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from sorbitolSOR	Acid from L-rhamnoseRHA	Acid from trehaloseTRE	CatalaseCAT	Spores presentSPOR	GramGRAM	Morphology coccus="+" rod="-"COCC
[Ref.: #845]	2221	-	-	+	-	-	-	+	-	-	-	+	+	-	-	-	-	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #845]	13310	-	+	-	-	-	+/-	+/-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123273]	13422	-	-	+	+	-	+	-	-	+	-	-	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #845]	Sample type/isolated from	gas gangrene

[Ref.: #123273]	Sample type/isolated from	Gas gangrene
[Ref.: #123273]	Isolation date	1920
* marker position based on {}

Isolation sources categories

#Infection

#Disease

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #845]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #123273]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Clostridium sporogenes strain FDAARGOS_1470	1509.215	complete	PATRIC	1509 tax ID	*
[Ref.: #66792]	Clostridium sporogenes NCTC 532	GCA_011019475	contig	GenBank	1509 tax ID	*
[Ref.: #66792]	Clostridium sporogenes strain ATCC 19404	1509.40	wgs	PATRIC	1509 tax ID	*
[Ref.: #66792]	Clostridium sporogenes strain FDAARGOS_1470	1509.207	complete	PATRIC	1509 tax ID	*
[Ref.: #66792]	Clostridium sporogenes strain NCTC 532	1509.155	wgs	PATRIC	1509 tax ID	*
[Ref.: #66792]	Clostridium sporogenes ATCC 19404	2724679562	draft	JGI IMG/M	1509 tax ID	*

[Ref.: #845]

GC-content

27.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Clostridium sporogenes strain FDAARGOS_1470 strain Not applicable PATRIC: 1509.207
[Ref.: #69481]	spore-forming	yes	95	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Clostridium sporogenes strain FDAARGOS_1470 strain Not applicable PATRIC: 1509.207
[Ref.: #69480]	motile	yes	84.357	no
[Ref.: #69480]	gram-positive	yes	91.36	no
[Ref.: #69480]	anaerobic	yes	97.662	yes
[Ref.: #69480]	aerobic	no	97.318	no
[Ref.: #69480]	halophile	no	79.618	no
[Ref.: #69480]	spore-forming	yes	93.467	no
[Ref.: #69480]	thermophile	no	96.91	yes
[Ref.: #69480]	glucose-util	yes	86.491	no
[Ref.: #69480]	flagellated	yes	53.307	no
[Ref.: #69480]	glucose-ferment	yes	64.543	no

Availability in culture collections External links

[Ref.: #845]

Culture collection no.

DSM 1664, ATCC 19404, CIP 79.3, NCIB 532, NCTC 532, WDCM 00008, NCIMB 532, LMG 10143, CCM 4409, CECT 485, CMCC (B) 64941

[Ref.: #72240]

SI-ID 1974

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Feathery outbrusts of the colonies of Clostridium sporogenes containing chain forming mutants after ultraviolet irradiation.	Shaikh MR, Lominski I	Zentralbl Bakteriol Orig A		1975	*
Pathogenicity	Induction of chain formation in Clostridium sporogenes by suramin.	Rafi Shaikh M, Lominski I	Zentralbl Bakteriol Orig A		1975	*

References

#845	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1664

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#36205 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68367

Automatically annotated from API 20A .

#68380

Automatically annotated from API rID32A .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72240

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID1974.1 )

#123273 Collection of Institut Pasteur ; Curators of the CIP; CIP 79.3
* These data were automatically processed and therefore are not curated

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Clostridium sporogenes strain FDAARGOS_1470 strain Not applicable

Clostridium sporogenes strain FDAARGOS_1470 strain Not applicable

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