Name: Methanothrix thermoacetophila PT
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 7050

Type strain:

Species: Methanothrix thermoacetophila

Strain Designation: PT

Culture col. no.: DSM 6194, JCM 14653, NBRC 101360

Strain history: NBRC 101360 <-- Y. Kamagata P_T.

NCBI tax ID(s): 2224 (species)

SI-ID 48117

DSM 6194, NBRC 101360, JCM 14653

Special Collections

DSMZ
JCM

Other strains from Methanothrix thermoacetophila

Section

Methanothrix thermoacetophila PT is an anaerobe, Gram-negative archaeon that was isolated from sludge, thermophilic anaerobic digester.

Gram-negative
anaerobe
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	"Methanomicrobia"
	Order	*Methanosarcinales*
	Family	*Methanotrichaceae*
	Genus	*Methanothrix*
	Species	**Methanothrix thermoacetophila**
	Full Scientific Name (LPSN)	Methanothrix thermoacetophila corrig. Nozhevnikova and Chudina 1988

	Synonym	Methanothrix thermophila
	Synonym	Methanosaeta thermophila
	Synonym	Methanothrix thermoacetophilia
	Synonym	Methanosaeta thermoacetophila

Information on morphological and physiological properties Morphology

[Ref.: #2522]

Incubation period

3-7 days

Multimedia content

[Ref.: #2522]

Caption:

Medium 334a with strain-specific modifications, 55°C

[Ref.: #2522]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2522]

Culture medium

METHANOTHRIX SOEHNGENII MEDIUM (DSMZ Medium 334a)

[Ref.: #2522]

Culture medium growth

[Ref.: #2522]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2522]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #2522]			growth	55 °C
[Ref.: #67770]			growth	55 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #2522]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %91.616

[Ref.: #69480]

Ability of spore formation

no Confidence in %95.136

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2522]	Sample type/isolated from	sludge, thermophilic anaerobic digester
[Ref.: #2522]	Country	Japan
[Ref.: #2522]	Country ISO 3 Code	JPN
[Ref.: #2522]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Digested sludge from a thermophilic anaerobic digestor
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Anaerobic digestor
#Environmental	#Terrestrial	#Mud (Sludge)
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB071701 (>99% sequence identity) for Methanothrix thermoacetophila subclade from Microbeatlas

Aquatic Samples	54
Soil Samples	1
Animal Samples	3
Plant Samples
Total Samples	58

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2522]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Methanosaeta thermophila PT gene for 16S rRNA, partial sequence	AB071701	1403	GenBank ENA	349307 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanothrix thermoacetophila PT	GCA_000014945	complete	GenBank ENA	349307 tax ID	*
[Ref.: #66792]	Methanothrix thermoacetophila PT	639633038	complete	JGI IMG/M	349307 tax ID	*
[Ref.: #66792]	Methanosaeta thermophila PT	349307.15	complete	PATRIC	349307 tax ID	*

[Ref.: #2522]	GC-content	52.7 mol%
[Ref.: #67770]	GC-content	52.7 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanothrix thermoacetophila PT PT NCBI: GCA_000014945.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	66.565	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	91.616	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	91.868	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	95.136	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	57.636	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	82.25	no

Availability in culture collections External links

[Ref.: #2522]

Culture collection no.

DSM 6194, JCM 14653, NBRC 101360

[Ref.: #76509]

SI-ID 48117

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanosaeta harundinacea sp. nov., a novel acetate-scavenging methanogen isolated from a UASB reactor.	Ma K, Liu X, Dong X	Int J Syst Evol Microbiol	10.1099/ijs.0.63887-0	2006	*
Phylogeny	Bioaugmentation with methanogens cultured in a micro-aerobic microbial community for overloaded anaerobic digestion recovery.	Hua B, Cai Y, Cui Z, Wang X	Anaerobe	10.1016/j.anaerobe.2022.102603	2022	*

References

#2522

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6194

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76509

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID48117.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Methanothrix thermoacetophila in external resources:
SILVA
BRENDA
PANGAEA
GBIF

Methanothrix thermoacetophila PT PT

Change proposal

Change proposal