Methanothrix thermoacetophila (DSM 6194, PT, NBRC 101360)

Name: Methanothrix thermoacetophila (DSM 6194, PT, NBRC 101360)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7050

Type strain

Strain Designation PT

Culture col. no. DSM 6194 PT NBRC 101360 JCM 14653

NCBI tax ID(s) 2224

Special Collections DSMZ JCM

History <- Y. Kamagata, Fermentation Research Institute, Tsukuba, Ibaraki, Japan; PT <- Y. Kamagata and E. Mikami {1988} NBRC 101360 <-- Y. Kamagata P_T.

Links

Methanothrix thermoacetophila PT is an anaerobe, thermophilic prokaryote that was isolated from sludge, thermophilic anaerobic digester.

anaerobe thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 48117

DSM 6194,NBRC 101360,JCM 14653

@ref 20215

Last LPSN update 2026-02-09 11:19:22

Domain Archaea

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanosarcinales

Family Methanotrichaceae

Genus Methanothrix

Species Methanothrix thermoacetophila

Full scientific name Methanothrix thermoacetophila corrig. Nozhevnikova and Chudina 1988

Synonyms (4)

Methanothrix thermophila
Methanothrix thermoacetophilia
Methanosaeta thermophila
Methanosaeta thermoacetophila

BacDive ID	Other strains from **Methanothrix thermoacetophila** (2)	Type strain
7049	M. thermoacetophila Z-517, DSM 4774, VKM B-1831 (type strain)
7048	M. thermoacetophila CALS-1, DSM 3870

Morphology

Colony morphology

2522

Incubation period3-7 days

Multimedia

@ref:	2522
multimedia content:	DSM_6194.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_6194.jpg
caption:	Medium 334a with strain-specific modifications, 55°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
67770	positive	growth	55	thermophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
2522	anaerobe
125438	anaerobe	91.616

Spore formation

@ref	Spore formation	Confidence
125438		95.136

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Biodegradation	#Anaerobic digestor
#Environmental	#Terrestrial	#Mud (Sludge)
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
2522	sludge, thermophilic anaerobic digester	Japan	JPN	Asia
67770	Digested sludge from a thermophilic anaerobic digestor

Taxonmaps

69479

Global distribution of 16S sequence AB071701 (>99% sequence identity) for Methanothrix thermoacetophila subclade from Microbeatlas

Aquatic Samples	54
Soil Samples	1
Animal Samples	3
Plant Samples
Total Samples	58

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2522	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1494v1 assembly for Methanothrix thermoacetophila PT	complete	GCA_000014945	349307.15	639633038	349307	98.67

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Methanosaeta thermophila PT gene for 16S rRNA, partial sequence	AB071701	1403		349307

GC content

@ref	GC-content (mol%)	Method
2522	52.7
67770	52.7	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methanothrix thermoacetophila PT
IMG: 639633038

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	75.40	no
125439	motility	BacteriaNetⓘ	yes	79.90	no
125439	gram_stain	BacteriaNetⓘ	variable	65.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	86.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanothrix thermoacetophila PT PT
NCBI: GCA_000014945.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	66.57	no
125438	anaerobic	anaerobicⓘ	yes	91.62	yes
125438	aerobic	aerobicⓘ	no	91.87	yes
125438	spore-forming	spore-formingⓘ	no	95.14	no
125438	thermophilic	thermophileⓘ	no	57.64	no
125438	flagellated	motile2+ⓘ	no	82.25	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Methane production by Methanothrix thermoacetophila via direct interspecies electron transfer with Geobacter metallireducens.	Zhou J, Smith JA, Li M, Holmes DE.	mBio	10.1128/mbio.00360-23	2023
Phylogeny	Bioaugmentation with methanogens cultured in a micro-aerobic microbial community for overloaded anaerobic digestion recovery.	Hua B, Cai Y, Cui Z, Wang X	Anaerobe	10.1016/j.anaerobe.2022.102603	2022
Phylogeny	Characterization of three thermophilic strains of Methanothrix ("Methanosaeta") thermophila sp. nov. and rejection of Methanothrix ("Methanosaeta") thermoacetophila.	Kamagata Y, Kawasaki H, Oyaizu H, Nakamura K, Mikami E, Endo G, Koga Y, Yamasato K.	Int J Syst Bacteriol	10.1099/00207713-42-3-463	1992
Phylogeny	Methanosaeta harundinacea sp. nov., a novel acetate-scavenging methanogen isolated from a UASB reactor.	Ma K, Liu X, Dong X	Int J Syst Evol Microbiol	10.1099/ijs.0.63887-0	2006

References

#2522	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6194
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanothrix thermoacetophila (DSM 6194, PT, NBRC 101360)

Name and taxonomic classification

Morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000014945

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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