Strain identifier

BacDive ID: 7050

Type strain: No

Species: Methanothrix thermoacetophila

Strain Designation: PT

Strain history: NBRC 101360 <-- Y. Kamagata PT.

NCBI tax ID(s): 2224 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 2522

BacDive-ID: 7050

DSM-Number: 6194

keywords: genome sequence, 16S sequence, Archaea, anaerobe, thermophilic

description: Methanothrix thermoacetophila PT is an anaerobe, thermophilic archaeon that was isolated from sludge, thermophilic anaerobic digester.

NCBI tax id

  • NCBI tax id: 2224
  • Matching level: species

strain history

@refhistory
2522<- Y. Kamagata, Fermentation Research Institute, Tsukuba, Ibaraki, Japan; PT <- Y. Kamagata and E. Mikami {1988}
67770NBRC 101360 <-- Y. Kamagata PT.

doi: 10.13145/bacdive7050.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanotrichaceae
  • genus: Methanothrix
  • species: Methanothrix thermoacetophila
  • full scientific name: Methanothrix thermoacetophila corrig. Nozhevnikova and Chudina 1988

  • synonyms

    @refsynonym
    20215Methanothrix thermophila
    20215Methanosaeta thermophila
    20215Methanothrix thermoacetophilia
    20215Methanosaeta thermoacetophila

@ref: 2522

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosaetaceae

genus: Methanothrix

species: Methanothrix thermoacetophila

full scientific name: Methanothrix thermoacetophila Nozhevnikova and Chudina 1988

strain designation: PT

type strain: no

Morphology

colony morphology

  • @ref: 2522
  • incubation period: 3-7 days

multimedia

  • @ref: 2522
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_6194.jpg
  • caption: Medium 334a with strain-specific modifications, 55°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

  • @ref: 2522
  • name: METHANOTHRIX SOEHNGENII MEDIUM (DSMZ Medium 334a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/334a
  • composition: Name: METHANOTHRIX SOEHNGENII MEDIUM (DSMZ Medium 334a; with strain-specific modifications) Composition: Na-acetate 6.8 g/l Na2CO3 1.25 g/l Na2HPO4 x 2 H2O 0.86 g/l Methanol 0.792 g/l Na2S x 9 H2O 0.5 g/l NaCl 0.3 g/l NH4Cl 0.3 g/l KH2PO4 0.22 g/l 2-Mercaptoethanesulfonate 0.14 g/l Nitrilotriacetic acid 0.128 g/l CaCl2 x 2 H2O 0.11 g/l MgCl2 x 6 H2O 0.1 g/l FeCl3 x 6 H2O 0.0135 g/l Butyl vinyl ether 0.005 g/l L-alpha-Phosphatidylcholine 0.005 g/l NiCl2 x 6 H2O 0.0012 g/l MnCl2 x 4 H2O 0.001 g/l ZnCl2 0.001 g/l Na2-EDTA 0.000999999 g/l Sodium resazurin 0.0005 g/l CuCl2 0.0003 g/l CoCl2 x 6 H2O 0.0003 g/l Na2MoO4 x 2 H2O 0.0003 g/l Na2SeO3 x 5 H2O 0.0003 g/l H3BO3 0.0001 g/l Pyridoxine hydrochloride 0.0001 g/l (DL)-alpha-Lipoic acid 5e-05 g/l Riboflavin 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l Nicotinic acid 5e-05 g/l Thiamine HCl 5e-05 g/l Biotin 2e-05 g/l Folic acid 2e-05 g/l Vitamin B12 1e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
2522positivegrowth55thermophilic
67770positivegrowth55thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 2522
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
2522sludge, thermophilic anaerobic digesterJapanJPNAsia
67770Digested sludge from a thermophilic anaerobic digestor

isolation source categories

Cat1Cat2Cat3
#Engineered#Biodegradation#Anaerobic digestor
#Environmental#Terrestrial#Mud (Sludge)
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_188479.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17997;96_69193;97_90295;98_122722;99_188479&stattab=map
  • Last taxonomy: Methanothrix thermoacetophila subclade
  • 16S sequence: AB071701
  • Sequence Identity:
  • Total samples: 58
  • soil counts: 1
  • aquatic counts: 54
  • animal counts: 3

Safety information

risk assessment

  • @ref: 2522
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Methanosaeta thermophila PT gene for 16S rRNA, partial sequence
  • accession: AB071701
  • length: 1403
  • database: ena
  • NCBI tax ID: 349307

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methanothrix thermoacetophila PTGCA_000014945completencbi349307
66792Methanothrix thermoacetophila PT639633038completeimg349307
66792Methanosaeta thermophila PT349307.15completepatric349307

GC content

@refGC-contentmethod
252252.7
6777052.7high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno90.742no
flagellatedno97.384no
gram-positiveno96.947no
anaerobicyes98.935no
halophileno87.39no
spore-formingno96.246no
glucose-utilyes73.829no
aerobicno95.812yes
thermophileyes92.806yes
glucose-fermentno63.452no

External links

@ref: 2522

culture collection no.: DSM 6194, JCM 14653, NBRC 101360

straininfo link

  • @ref: 76509
  • straininfo: 48117

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16403877Methanosaeta harundinacea sp. nov., a novel acetate-scavenging methanogen isolated from a UASB reactor.Ma K, Liu X, Dong XInt J Syst Evol Microbiol10.1099/ijs.0.63887-02006Acetates/metabolism, Anaerobiosis, Base Composition, Beer, *Bioreactors, China, Culture Media, DNA Restriction Enzymes/genetics, Hydrogen-Ion Concentration, Methane/metabolism, Methanosarcinaceae/chemistry/*classification/isolation & purification/physiology, Molecular Sequence Data, Oxidoreductases/genetics/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Sewage/*microbiology, Species SpecificityMetabolism
Phylogeny35709936Bioaugmentation with methanogens cultured in a micro-aerobic microbial community for overloaded anaerobic digestion recovery.Hua B, Cai Y, Cui Z, Wang XAnaerobe10.1016/j.anaerobe.2022.1026032022Anaerobiosis, Bioreactors, *Euryarchaeota/genetics, Methane, *Microbiota, RNA, Ribosomal, 16S/genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2522Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6194)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6194
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76509Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48117.1StrainInfo: A central database for resolving microbial strain identifiers