Methanoculleus bourgensis (DSM 2772, ATCC 35293, OCM 52)

Name: Methanoculleus bourgensis (DSM 2772, ATCC 35293, OCM 52)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7015

Type strain

Strain Designation RC/ER

Culture col. no. DSM 2772 ATCC 35293 OCM 52 RC/ER

NCBI tax ID(s) 83986

Special Collections DSMZ

Links

Methanoculleus bourgensis RC/ER is an anaerobe, mesophilic prokaryote that was isolated from freshwater sediment.

anaerobe mesophilic

Name and taxonomic classification

SI-ID 40978

ATCC 35293,DSM 2772

@ref 20215

Last LPSN update 2025-12-16 09:48:28

Domain Methanobacteriati

Phylum Methanobacteriota

Class "Methanomicrobia"

Order Methanomicrobiales

Family Methanomicrobiaceae

Genus Methanoculleus

Species Methanoculleus bourgensis

Full scientific name Methanoculleus bourgensis corrig. (Ollivier et al. 1986) Maestrojuán et al. 1990

Synonyms (5)

Methanogenium bourgense
Methanogenium olentangyi
Methanoculleus oldenburgensis
Methanoculleus olentangyi
Methanoculleus bourgense

BacDive ID	Other strains from **Methanoculleus bourgensis** (4)	Type strain
7016	M. bourgensis MS2, DSM 3045, ATCC 43281, OCM 15 (type strain)
7017	M. bourgensis LX1, DSM 4307, ATCC 43575, OCM 24
7018	M. bourgensis CB 1, DSM 6216
7019	M. bourgensis EBac, DSM 8220

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1218	METHANOCULLEUS CHIKUGOENSIS MEDIUM (DSMZ Medium 287)		Medium recipe at MediaDive	Name: METHANOCULLEUS CHIKUGOENSIS MEDIUM (DSMZ Medium 287) Composition: NaHCO3 4.9456 g/l NH4Cl 2.67062 g/l Na-acetate 2.4728 g/l Na2S x 9 H2O 0.296736 g/l KH2PO4 0.296736 g/l (NH4)2SO4 0.296736 g/l K2HPO4 0.296736 g/l L-Cysteine HCl x H2O 0.296736 g/l CaCl2 x H2O 0.138477 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l FeSO4 x 7 H2O 0.00098912 g/l CaCl2 x 2 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l CuSO4 x 5 H2O 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l H3BO3 9.8912e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Biotin 1.97824e-05 g/l Folic acid 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
1218	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

1218

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Sediment
#Environmental	#Aquatic	#Freshwater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
1218	freshwater sediment	Ohio, Olentangy river	USA	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1218	1	Risk group (German classification) According to TRBA 466

Sequence information

GC content

1218

GC-content (mol%)54.4

Genome-based predictions

Literature

References

#1218	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2772
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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