Methanofollis formosanus (DSM 15483, OCM 789, ML15)

Name: Methanofollis formosanus (DSM 15483, OCM 789, ML15)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7013

Type strain

Strain Designation ML15, N2M9704

Culture col. no. DSM 15483 OCM 789 ML15 N2M9704

NCBI tax ID(s) 299308

Special Collections DSMZ

Links

Methanofollis formosanus ML15 is an anaerobe, mesophilic prokaryote that was isolated from aquaculture fish pond.

anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 138014

DSM 15483

@ref 20215

Last LPSN update 2025-12-16 09:48:28

Domain Methanobacteriati

Phylum Methanobacteriota

Class "Methanomicrobia"

Order Methanomicrobiales

Family Methanomicrobiaceae

Genus Methanofollis

Species Methanofollis formosanus

Full scientific name Methanofollis formosanus Wu et al. 2005

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
5952	METHANOFOLLIS MB MEDIUM (5 G/L NACL) (DSMZ Medium 924a)		Medium recipe at MediaDive	Name: METHANOFOLLIS MB MEDIUM (5 g/l NaCl) (DSMZ Medium 924a; with strain-specific modifications) Composition: Na-formate 6.72601 g/l NaHCO3 3.95648 g/l Trypticase peptone 1.97824 g/l Yeast extract 1.97824 g/l Na acetate 1.58259 g/l NH4Cl 0.98912 g/l MgCl2 x 6 H2O 0.98912 g/l KCl 0.49456 g/l K2HPO4 0.395648 g/l L-Cysteine HCl x H2O 0.24728 g/l Na2S x 9 H2O 0.24728 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l H3BO3 9.8912e-05 g/l CuSO4 x 5 H2O 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
5952	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

5952

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Aquaculture
#Environmental	#Aquatic	#Pond (small)
#Host	#Fishes	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
5952	aquaculture fish pond	near Wang-gong	Taiwan, Province of China	TWN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AY186542 (>99% sequence identity) for Methanofollis formosanus subclade from Microbeatlas

Aquatic Samples	39
Soil Samples	10
Animal Samples	30
Plant Samples	4
Total Samples	83

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5952	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1963374v1 assembly for Methanofollis formosanus ML15	chromosome	GCA_019633745	299308.3	2982334219	299308	86.01

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5952	Methanofollis formosanus 16S ribosomal RNA gene, partial sequence	AY186542	1287		299308

GC content

5952

GC-content (mol%)58.4

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanofollis formosanus strain ML15
PATRIC: 299308.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	71.25	no
125438	anaerobic	anaerobicⓘ	yes	88.96	yes
125438	spore-forming	spore-formingⓘ	no	87.03	no
125438	aerobic	aerobicⓘ	no	88.53	no
125438	thermophilic	thermophileⓘ	no	75.90	yes
125438	flagellated	motile2+ⓘ	no	83.31	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of Methanofollis formosanus DSM 15483(T), Isolated from an Aquaculture Fish Pond.	Chen SC, Wu CH, You YT, Wu SY, Liao CH, Wang X, Li H, Guo Y, You J, Qiu W	Microbiol Resour Announc	10.1128/mra.00068-22	2022
Phylogeny	Methanofollis formosanus sp. nov., isolated from a fish pond.	Wu SY, Chen SC, Lai MC	Int J Syst Evol Microbiol	10.1099/ijs.0.63475-0	2005

References

#5952	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15483
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanofollis formosanus (DSM 15483, OCM 789, ML15)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_019633745

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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