Name: Methanofollis formosanus ML15
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7013

Type strain:

Species: Methanofollis formosanus

Strain Designation: ML15, N2M9704

Culture col. no.: DSM 15483, OCM 789

Strain history: <- M.-C. Lai, National Chung Hsing Univ., Dept. of Life Scs., Taiwan; ML15 <- M.-C. Lai and S.-C. Chen; N2M9704 {2000}

NCBI tax ID(s): 299308 (species)

SI-ID 138014

DSM 15483

Section

Methanofollis formosanus ML15 is an anaerobe, mesophilic, Gram-negative archaeon that was isolated from aquaculture fish pond.

Gram-negative
anaerobe
mesophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	"Methanomicrobia"
	Order	*Methanomicrobiales*
	Family	*Methanomicrobiaceae*
	Genus	*Methanofollis*
	Species	**Methanofollis formosanus**
	Full Scientific Name (LPSN)	Methanofollis formosanus Wu et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.982

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5952]

Culture medium

METHANOFOLLIS MB MEDIUM (5 G/L NACL) (DSMZ Medium 924a)

[Ref.: #5952]

Culture medium growth

[Ref.: #5952]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5952]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #5952]			growth	37 °C

[Ref.: #5952]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5952]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.68

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.907

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5952]	Sample type/isolated from	aquaculture fish pond
[Ref.: #5952]	Geographic location (country and/or sea, region)	Taiwan, near Wang-gong
[Ref.: #5952]	Country	China
[Ref.: #5952]	Country ISO 3 Code	CHN
[Ref.: #5952]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Aquaculture
#Environmental	#Aquatic	#Pond (small)
#Host	#Fishes	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY186542 (>99% sequence identity) for Methanofollis formosanus subclade from Microbeatlas

Aquatic Samples	39
Soil Samples	10
Animal Samples	30
Plant Samples	4
Total Samples	83

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5952]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5952]	Methanofollis formosanus 16S ribosomal RNA gene, partial sequence	AY186542	1287	ENA	299308 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanofollis formosanus ML15	GCA_019633745	chromosome	GenBank	299308 tax ID	*
[Ref.: #66792]	Methanofollis formosanus strain ML15	299308.3	complete	PATRIC	299308 tax ID	*

[Ref.: #5952]

GC-content

58.4 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Methanofollis formosanus ML15 NCBI: GCA_019633745.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanofollis formosanus strain ML15 PATRIC: 299308.3
[Ref.: #69480]	gram-positive	no	97.758	no
[Ref.: #69480]	anaerobic	yes	99.163	yes
[Ref.: #69480]	halophile	no	79.63	no
[Ref.: #69480]	spore-forming	no	95.529	no
[Ref.: #69480]	glucose-util	yes	72.662	no
[Ref.: #69480]	motile	no	93.374	no
[Ref.: #69480]	aerobic	no	96.501	yes
[Ref.: #69480]	thermophile	no	85.557	no
[Ref.: #69480]	flagellated	no	98.18	no
[Ref.: #69480]	glucose-ferment	no	69.257	no

Availability in culture collections External links

[Ref.: #5952]

Culture collection no.

DSM 15483, OCM 789

[Ref.: #76474]

SI-ID 138014

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of Methanofollis formosanus DSM 15483(T), Isolated from an Aquaculture Fish Pond.	Chen SC, Wu CH, You YT, Wu SY, Liao CH, Wang X, Li H, Guo Y, You J, Qiu W	Microbiol Resour Announc	10.1128/mra.00068-22	2022	*
Phylogeny	Methanofollis formosanus sp. nov., isolated from a fish pond.	Wu SY, Chen SC, Lai MC	Int J Syst Evol Microbiol	10.1099/ijs.0.63475-0	2005	*

References

#5952

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15483

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76474

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID138014.1 )

* These data were automatically processed and therefore are not curated

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Methanofollis formosanus ML15

Methanofollis formosanus strain ML15

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