Methanoculleus bourgensis (DSM 4307, ATCC 43575, OCM 24)

Name: Methanoculleus bourgensis (DSM 4307, ATCC 43575, OCM 24)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7017

Type strain

Strain Designation LX1

Culture col. no. DSM 4307 ATCC 43575 OCM 24 LX1

NCBI tax ID(s) 83986

Special Collections DSMZ

History <- L. Xun, LX1

Links

Methanoculleus bourgensis LX1 is an anaerobe, mesophilic prokaryote that was isolated from propionate enrichment inoculated from mesophilic anaerobic digester.

anaerobe mesophilic

Name and taxonomic classification

SI-ID 47828

DSM 4307,ATCC 43575

@ref 20215

Last LPSN update 2026-02-09 11:19:22

Domain Archaea

Phylum Methanobacteriota

Class "Methanomicrobia"

Order Methanomicrobiales

Family Methanomicrobiaceae

Genus Methanoculleus

Species Methanoculleus bourgensis

Full scientific name Methanoculleus bourgensis corrig. (Ollivier et al. 1986) Maestrojuán et al. 1990

Synonyms (5)

Methanogenium bourgense
Methanogenium olentangyi
Methanoculleus oldenburgensis
Methanoculleus olentangyi
Methanoculleus bourgense

BacDive ID	Other strains from **Methanoculleus bourgensis** (4)	Type strain
7016	M. bourgensis MS2, DSM 3045, ATCC 43281, OCM 15 (type strain)
7015	M. bourgensis RC/ER, DSM 2772, ATCC 35293, OCM 52
7018	M. bourgensis CB 1, DSM 6216
7019	M. bourgensis EBac, DSM 8220

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1649	METHANOBACTERIUM MEDIUM (DSMZ Medium 119)		Medium recipe at MediaDive	Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
1649	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

1649

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Laboratory	#Lab enrichment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
1649	propionate enrichment inoculated from mesophilic anaerobic digester	California, Los Angeles	USA	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1649	1	Risk group (German classification) According to TRBA 466

Sequence information

GC content

1649

GC-content (mol%)59.5

Genome-based predictions

Literature

References

#1649	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4307
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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