General

@ref: 1218

BacDive-ID: 7015

DSM-Number: 2772

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanoculleus bourgensis RC/ER is an anaerobe, mesophilic archaeon that was isolated from freshwater sediment.

NCBI tax id

• NCBI tax id: 83986
• Matching level: species

strain history

• @ref: 1218
• history: <- J.I. Frea, strain RC/ER

doi: 10.13145/bacdive7015.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanomicrobia
• order: Methanomicrobiales
• family: Methanomicrobiaceae
• genus: Methanoculleus
• species: Methanoculleus bourgensis
• full scientific name: Methanoculleus bourgensis corrig. (Ollivier et al. 1986) Maestrojuán et al. 1990

• synonyms

  @ref	synonym
  20215	Methanogenium olentangyi
  20215	Methanoculleus oldenburgensis
  20215	Methanoculleus olentangyi
  20215	Methanoculleus bourgense
  20215	Methanogenium bourgense

@ref: 1218

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanomicrobiales

family: Methanomicrobiaceae

genus: Methanoculleus

species: Methanoculleus bourgensis

full scientific name: Methanoculleus bourgensis (Ollivier et al. 1986) Maestrojuán et al. 1990 emend. Asakawa and Nagaoka 2003

strain designation: RC/ER

type strain: no

Culture and growth conditions

culture medium

• @ref: 1218
• name: METHANOGENIUM OLENTANGYI MEDIUM (DSMZ Medium 287)
• growth: yes
• link: https://mediadive.dsmz.de/medium/287
• composition: Name: METHANOCULLEUS CHIKUGOENSIS MEDIUM (DSMZ Medium 287) Composition: NaHCO3 4.9456 g/l NH4Cl 2.67062 g/l Na-acetate 2.4728 g/l Na2S x 9 H2O 0.296736 g/l KH2PO4 0.296736 g/l (NH4)2SO4 0.296736 g/l K2HPO4 0.296736 g/l L-Cysteine HCl x H2O 0.296736 g/l CaCl2 x H2O 0.138477 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l FeSO4 x 7 H2O 0.00098912 g/l CaCl2 x 2 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l CuSO4 x 5 H2O 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l H3BO3 9.8912e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Biotin 1.97824e-05 g/l Folic acid 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

culture temp

• @ref: 1218
• growth: positive
• type: growth
• temperature: 37
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 1218
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 1218
• sample type: freshwater sediment
• geographic location: Ohio, Olentangy river
• country: USA
• origin.country: USA
• continent: North America

isolation source categories

Safety information

risk assessment

• @ref: 1218
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 20218
• description: Enterococcus faecalis clone OCM052 16S ribosomal RNA gene, partial sequence
• accession: GU417236
• length: 457
• database: ena
• NCBI tax ID: 1351

GC content

• @ref: 1218
• GC-content: 54.4

External links

@ref: 1218

culture collection no.: DSM 2772, ATCC 35293, OCM 52

straininfo link

• @ref: 76475
• straininfo: 40978

Reference