Methanoculleus bourgensis (DSM 6216, CB 1)

Name: Methanoculleus bourgensis (DSM 6216, CB 1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7018

Type strain

Strain Designation CB 1

Culture col. no. DSM 6216 CB 1

NCBI tax ID(s) 83986

Special Collections DSMZ

History <- K.-H. Blotevogel, Univ. Oldenburg, Germany; CB1 <- K.-H. Blotevogel {1989}

Links

Methanoculleus bourgensis CB 1 is an anaerobe, mesophilic prokaryote that was isolated from sediment.

anaerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 48126

DSM 6216

@ref 20215

Last LPSN update 2026-02-09 11:19:22

Domain Archaea

Phylum Methanobacteriota

Class "Methanomicrobia"

Order Methanomicrobiales

Family Methanomicrobiaceae

Genus Methanoculleus

Species Methanoculleus bourgensis

Full scientific name Methanoculleus bourgensis corrig. (Ollivier et al. 1986) Maestrojuán et al. 1990

Synonyms (5)

Methanogenium bourgense
Methanogenium olentangyi
Methanoculleus oldenburgensis
Methanoculleus olentangyi
Methanoculleus bourgense

BacDive ID	Other strains from **Methanoculleus bourgensis** (4)	Type strain
7016	M. bourgensis MS2, DSM 3045, ATCC 43281, OCM 15 (type strain)
7015	M. bourgensis RC/ER, DSM 2772, ATCC 35293, OCM 52
7017	M. bourgensis LX1, DSM 4307, ATCC 43575, OCM 24
7019	M. bourgensis EBac, DSM 8220

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
2541	METHANOBACTERIUM MEDIUM (DSMZ Medium 119)		Medium recipe at MediaDive	Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119; with strain-specific modifications) Composition: NaHCO3 3.98804 g/l Na-acetate 2.99103 g/l Na-formate 1.99402 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
2541	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

2541

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Sediment
#Environmental	#Terrestrial	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
2541	sediment	Oldenburg, Hunte river	Germany	DEU	Europe

Taxonmaps

69479

Global distribution of 16S sequence AB065298 (>99% sequence identity) for Methanoculleus from Microbeatlas

Aquatic Samples	34
Soil Samples	4
Animal Samples
Plant Samples	3
Total Samples	41

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2541	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Methanoculleus bourgensis gene for 16S rRNA	AB065298	1431		83986

GC content

2541

GC-content (mol%)48.5

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Characterization of Blf4, an Archaeal Lytic Virus Targeting a Member of the Methanomicrobiales.	Weidenbach K, Wolf S, Kupczok A, Kern T, Fischer MA, Reetz J, Urbanska N, Kunzel S, Schmitz RA, Rother M.	Viruses	10.3390/v13101934	2021

References

#2541	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6216
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanoculleus bourgensis (DSM 6216, CB 1)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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