Enterobacter cloacae (DSM 3264, IFO 12935, NBRC 12935)

Name: Enterobacter cloacae (DSM 3264, IFO 12935, NBRC 12935)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 4360

Type strain

Strain Designation A2-4

Culture col. no. DSM 3264 IFO 12935 NBRC 12935 A2-4 CIP 103475

NCBI tax ID(s) 550

Special Collections DSMZ CIP

History DSMZ <- Institute For Fermentation, Osaka, Japan (IFO); see NBRC; IFO 12935 <- Y. Nakanji; A2-4 (Aerobacter cloacae) CIP <- 1992, DSM <- IFO <- Y. Nakanji: strain A2-4, Aerobacter cloacae

Links

Enterobacter cloacae A2-4 is a facultative anaerobe, Gram-negative, motile bacterium of the family Enterobacteriaceae.

Gram-negative motile rod-shaped facultative anaerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 13974

LMG 15556,CIP 103475,DSM 3264,IFO 12935,NBRC 12935

@ref 20215

Last LPSN update 2026-05-29 08:25:55

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Enterobacterales

Family Enterobacteriaceae

Genus Enterobacter

Species Enterobacter cloacae

Full scientific name Enterobacter cloacae (Jordan 1890) Hormaeche and Edwards 1960 (Approved Lists 1980)

Synonyms (3)

"Bacillus cloacae"
"Bacterium cloacae"
"Cloaca cloacae"

BacDive ID	Other strains from **Enterobacter cloacae** (62)	Type strain
4361	E. cloacae D 12, D12, DSM 6234, CCM 7931, CIP 104674
4363	E. cloacae A-8, DSM 26481, ATCC 23355, CCUG 33777, CECT ...
23915	E. cloacae WS 20940, DSM 30062
134563	E. cloacae B0025752, CIP 106422
134732	E. cloacae CIP 57.9
135801	E. cloacae P99, CIP 79.28, NCIMB 12091
136232	E. cloacae AmMS 204, CIP 103624, ATCC 49141
136527	E. cloacae 20/78, CIP 106471
136754	E. cloacae RIVM WR3, CIP 110806, NCTC 13168, WDCM 00200
136949	E. cloacae EN-117, CIP 108488
137003	E. cloacae CIP 60.22
138654	E. cloacae 1413B, CIP 105132
141188	E. cloacae CCUG 56
141652	E. cloacae CCUG 4426
144505	E. cloacae CCUG 25404
144506	E. cloacae CCUG 25407
144514	E. cloacae CCUG 25437
144515	E. cloacae CCUG 25442
144518	E. cloacae CCUG 25456
144520	E. cloacae CCUG 25464
144521	E. cloacae CCUG 25467
144863	E. cloacae CCUG 26899
145768	E. cloacae CCUG 29540, ATCC 29941
146596	E. cloacae CCUG 31552
146754	E. cloacae CCUG 32069
146836	E. cloacae CCUG 32203 B
146902	E. cloacae CCUG 32405
147301	E. cloacae CCUG 33336
147305	E. cloacae CCUG 33342
147579	E. cloacae CCUG 33830
147673	E. cloacae CCUG 33986, NCTC 11936
148432	E. cloacae CCUG 35438
148634	E. cloacae CCUG 35748
149083	E. cloacae CCUG 36835
149658	E. cloacae CCUG 38136
150146	E. cloacae CCUG 39095
150353	E. cloacae CCUG 39712
151275	E. cloacae CCUG 43598
151276	E. cloacae CCUG 43599
154273	E. cloacae CCUG 52947
155892	E. cloacae CCUG 59573
155911	E. cloacae CCUG 59627
157221	E. cloacae CCUG 70660, NCTC 13405
157222	E. cloacae CCUG 70661, NCTC 13406
157345	E. cloacae IMI 347357
157346	E. cloacae IMI 347358
157347	E. cloacae IMI 347359
157348	E. cloacae IMI 378896
157682	E. cloacae NRZ36863, DSM 106614
157683	E. cloacae ZW9, DSM 109592
157684	E. cloacae ZW32, DSM 109593
162811	E. cloacae JCM 20208, IAM 1562
162813	E. cloacae JCM 20225, IAM 1615
162814	E. cloacae JCM 20227, IAM 1624
172312	E. cloacae CIMB 04-7032, CRBIP17.69
172313	E. cloacae BM4439, CIP 111219
179112	E. cloacae IMET 10791, STI10791
177897	E. cloacae 1372, SF002772
178690	E. cloacae Hoechst 1321E, ST033630
178691	E. cloacae P99, ST033631
178692	E. cloacae Wiedemann IV3-25, ST033632
178693	E. cloacae Wiedemann IV3-26, ST033633

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
118312	negative	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
35553	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
118312	CIP Medium 3	Medium recipe at CIP
1333	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
1333	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

Culture temp

@ref	Growth	Type	Temperature (°C)
1333	positive	growth	30
35553	positive	growth	30
118312	positive	growth	5-41

Physiology and metabolism

Oxygen tolerance

118312

Oxygen tolerancefacultative anaerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
118312	16947 ChEBI	citrate	+	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	+	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	+	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
118312	17234 ChEBI	glucose	+	fermentation
118312	17234 ChEBI	glucose	+	degradation
68371	17754 ChEBI	glycerol	+	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	+	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
118312	17716 ChEBI	lactose	+	fermentation
118312	15792 ChEBI	malonate	+	assimilation
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
118312	29864 ChEBI	mannitol	+	fermentation
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	+	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	+	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
118312	17632 ChEBI	nitrate	+	reduction
118312	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	+	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
118312	132112 ChEBI	sodium thiosulfate	-	builds gas from
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
118312	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
118312	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
118312	15688 ChEBI	acetoin	+
118312	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
118312	alcohol dehydrogenase	+	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
118312	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
118312	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
118312	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
118312	ornithine decarboxylase	+	4.1.1.17
118312	oxidase	-
68382	trypsin	+	3.4.21.4	from API zym
118312	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
118312	-	+	+	+	-	+	+	-	+	-	+	+	+	+	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
118312	not determinedn.d.	+	-	-	+	+	+	-	-	-	+	+	+	+	-	+	+	+	+	+	-	+	+	-	+	+	+	+	+	+/-	+	+	+	-	-	+	+/-	-	-	+	+/-	+/-	-	-	-	-	-	-	+	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
118312	not determinedn.d.	+	+	+	+	+	-	+	+	+	+	+	+	-	+	+	+	+	+	+	+	+	+	+	+	-	-	-	-	-	+	-	+	+	+	+	+	+	-	-	+	-	+	-	+	+	-	-	-	-	+	+	+	-	+	+	+	+	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	-	+	-	-	-	+	-	-	+	+	+	+	+	+	-	-	-	-

Isolation, sampling and environmental information

Taxonmaps

69479

Global distribution of 16S sequence AB680341 (>99% sequence identity) for Enterobacterales from Microbeatlas

Aquatic Samples	888
Soil Samples	575
Animal Samples	11838
Plant Samples	920
Total Samples	14221

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
118312	2	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Enterobacter cloacae gene for 16S rRNA, partial sequence, strain: NBRC 12935	AB680341	1465		550

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Enterobacter cloacae from urinary tract infections: frequency, protein analysis, and antimicrobial resistance.	Elbehiry A, Al Shoaibi M, Alzahrani H, Ibrahem M, Moussa I, Alzaben F, Alsubki RA, Hemeg HA, Almutairi D, Althobaiti S, Alanazi F, Alotaibi SA, Almutairi H, Alzahrani A, Abu-Okail A.	AMB Express	10.1186/s13568-024-01675-7	2024
	mar Operon involved in multidrug resistance of Enterobacter aerogenes.	Chollet R, Bollet C, Chevalier J, Mallea M, Pages JM, Davin-Regli A.	Antimicrob Agents Chemother	10.1128/aac.46.4.1093-1097.2002	2002
Pathogenicity	Bactericidal activity of M14659 enhanced in low-iron environments.	Mochizuki H, Yamada H, Oikawa Y, Murakami K, Ishiguro J, Kosuzume H, Aizawa N, Mochida E.	Antimicrob Agents Chemother	10.1128/aac.32.11.1648	1988
	First Evidence of a Combination of Terpinen-4-ol and alpha-Terpineol as a Promising Tool against ESKAPE Pathogens.	Johansen B, Duval RE, Sergere JC.	Molecules	10.3390/molecules27217472	2022
	Synthesis and biological evaluation of new 2-azetidinones with sulfonamide structures.	Dragostin OM, Lupascu F, Vasile C, Mares M, Nastasa V, Moraru RF, Pieptu D, Profire L.	Molecules	10.3390/molecules18044140	2013

References

#1333	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3264
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#35553	; Curators of the CIP;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118312	Collection of Institut Pasteur ; Curators of the CIP; CIP 103475
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Enterobacter cloacae (DSM 3264, IFO 12935, NBRC 12935)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

API biotype100

Isolation, sampling and environmental information

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

Help

Social Media