Strain identifier
BacDive ID: 4360
Type strain:
Species: Enterobacter cloacae
Strain Designation: A2-4
Strain history: CIP <- 1992, DSM <- IFO <- Y. Nakanji: strain A2-4, Aerobacter cloacae
NCBI tax ID(s): 42895 (species)
General
@ref: 1333
BacDive-ID: 4360
DSM-Number: 3264
keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Enterobacter cloacae A2-4 is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Enterobacteriaceae.
NCBI tax id
- NCBI tax id: 42895
- Matching level: species
strain history
@ref | history |
---|---|
1333 | <- IFO <- Y. Nakanji, A2-4 (Aerobacter cloacae) |
118312 | CIP <- 1992, DSM <- IFO <- Y. Nakanji: strain A2-4, Aerobacter cloacae |
doi: 10.13145/bacdive4360.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Enterobacteriaceae
- genus: Enterobacter
- species: Enterobacter cloacae
- full scientific name: Enterobacter cloacae (Jordan 1890) Hormaeche and Edwards 1960 (Approved Lists 1980)
synonyms
@ref synonym 20215 Cloaca cloacae 20215 Bacillus cloacae 20215 Bacterium cloacae
@ref: 1333
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacteriales, not validated
family: Enterobacteriaceae
genus: Enterobacter
species: Enterobacter cloacae
full scientific name: Enterobacter sp.
strain designation: A2-4
type strain: no
Morphology
cell morphology
- @ref: 118312
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
1333 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
35553 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
118312 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
1333 | positive | growth | 30 | mesophilic |
35553 | positive | growth | 30 | mesophilic |
118312 | positive | growth | 5-41 |
Physiology and metabolism
oxygen tolerance
- @ref: 118312
- oxygen tolerance: facultative anaerobe
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | + | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | gentiobiose | + | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | raffinose | + | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | + | builds acid from | 27082 |
68371 | sucrose | + | builds acid from | 17992 |
68371 | melibiose | + | builds acid from | 28053 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | + | builds acid from | 17057 |
68371 | salicin | + | builds acid from | 17814 |
68371 | esculin | + | builds acid from | 4853 |
68371 | arbutin | + | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | + | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | + | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | + | builds acid from | 17924 |
68371 | D-mannitol | + | builds acid from | 16899 |
68371 | myo-inositol | + | builds acid from | 17268 |
68371 | galactitol | + | builds acid from | 16813 |
68371 | L-rhamnose | + | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | + | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | + | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | + | builds acid from | 65327 |
68371 | D-ribose | + | builds acid from | 16988 |
68371 | L-arabinose | + | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | + | builds acid from | 17754 |
118312 | mannitol | + | fermentation | 29864 |
118312 | citrate | + | carbon source | 16947 |
118312 | glucose | + | fermentation | 17234 |
118312 | lactose | + | fermentation | 17716 |
118312 | nitrate | + | reduction | 17632 |
118312 | nitrite | - | reduction | 16301 |
118312 | malonate | + | assimilation | 15792 |
118312 | sodium thiosulfate | - | builds gas from | 132112 |
118312 | glucose | + | degradation | 17234 |
antibiotic resistance
- @ref: 118312
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
- @ref: 118312
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
118312 | 15688 | acetoin | + | |
118312 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | alpha-galactosidase | + | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | + | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
118312 | oxidase | - | |
118312 | beta-galactosidase | + | 3.2.1.23 |
118312 | alcohol dehydrogenase | + | 1.1.1.1 |
118312 | catalase | + | 1.11.1.6 |
118312 | lysine decarboxylase | - | 4.1.1.18 |
118312 | ornithine decarboxylase | + | 4.1.1.17 |
118312 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
118312 | - | + | + | + | - | + | + | - | + | - | + | + | + | + | - | + | + | - | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
118312 | + | - | - | + | + | + | - | - | - | + | + | + | + | - | + | + | + | + | + | - | + | + | - | + | + | + | + | + | +/- | + | + | + | - | - | + | +/- | - | - | + | +/- | +/- | - | - | - | - | - | - | + | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
118312 | + | + | + | + | + | - | + | + | + | + | + | + | - | + | + | + | + | + | + | + | + | + | + | + | - | - | - | - | - | + | - | + | + | + | + | + | + | - | - | + | - | + | - | + | + | - | - | - | - | + | + | + | - | + | + | + | + | - | - | + | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | + | + | - | + | - | - | - | + | - | - | + | + | + | + | + | + | - | - | - | - |
Isolation, sampling and environmental information
taxonmaps
- @ref: 69479
- File name: preview.99_66.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_7;98_17;99_66&stattab=map
- Last taxonomy: Enterobacterales
- 16S sequence: AB680341
- Sequence Identity:
- Total samples: 14221
- soil counts: 575
- aquatic counts: 888
- animal counts: 11838
- plant counts: 920
Safety information
risk assessment
- @ref: 118312
- biosafety level: 2
- biosafety level comment: Risk group (French classification)
Sequence information
16S sequences
- @ref: 20218
- description: Enterobacter cloacae gene for 16S rRNA, partial sequence, strain: NBRC 12935
- accession: AB680341
- length: 1465
- database: ena
- NCBI tax ID: 550
External links
@ref: 1333
culture collection no.: DSM 3264, IFO 12935, NBRC 12935, CIP 103475
straininfo link
- @ref: 73851
- straininfo: 13974
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
1333 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3264) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3264 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
35553 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15276 | ||||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
73851 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID13974.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
118312 | Curators of the CIP | Collection of Institut Pasteur (CIP 103475) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103475 |